一种基于双工作电极-双通道的毛细管电泳电化学检测系统

报道了一种双工作电极-双通道毛细管电泳电化学检测系统，实现电导和安培 同时检测或者安培与安培检测联用，使两种方法相互补充，发挥各自的优势。其中 ，工作电极与检测池的制作工艺简单，操作简便，通过不锈钢针管和毛细管作为套 管，无需三维微调装置即可简单实现双工作电极的准确放置及分离毛细管与工作电 极的准确对接，并根据分析体系的需要采用不同类型的工作电极和检测器；同时采 用复式滤波电路解决了不同检测器之间的电场叠加对输出信号的干扰问题。采用该 装置可以同时检测复杂体系中的电活性和惰性物质，或同时测定只能氧化或只能还 原的物质，还可以对具有氧化还原性质的物质进行纯度的确证。将该装置应用于实 际样品的测定，节约了分析时间，提高了分析速度，扩大了检测范围，结果令人满 意。     

毛细管电泳电化学检测

综述了毛细管电泳的电化学检测,包括电位法、电导法和安培法检测的研究进展,重点讨论了电化学检测与毛细管电泳的耦联,并对电化学检测的原理及其应用进行了较详细的叙述,引用文献72篇。     

毛细管电泳多道电化学检测器的研制及其应用

报道了毛细管电泳多道电化学检测器的研制及其应用，安培检测器和电导检测器并联在同一毛细管电泳检测系统中，在同一缓冲体系，同一工作电极下对同一复杂的分析体系同时进行电导和安培检测；整个装置综合了电导检测和安培检测两种检测器的优点，性能优良，造价低廉，对实际样品的检测取得了令人满意的结果。     

Carbon Fiber-gold/mercury Dual-electrode Detection for Capillary Electrophoresis

Capillary electrophoresis (CE) has become a useful and powerful separation technique and has many applications. Electrochemical detection (ED) has been shown to be one of the most sensitive detection techniques available for use in CE. Dual-electrode detection can expand the applicability of CE-ED1-5, the materials such as gold/mercury1,3, carbon fiber2, Pt4 and Au3,5 have been used for the two electrodes in dual-electrodes. As is well known, the electrochemical response of different c…     

一种新型毛细管电泳柱端喷壁安培检测池

提出一种新型毛细管电泳柱端喷壁安培检测池。它制作简单，操作容易，具有很好的重现性和灵敏度。用这种安培检测池，可以不使用微动平台很容易地将工作电极与分离毛细管出口对准。为评价该安培检测池的分析性能，用毛细管电泳柱端安培检测法分别检测了酚、儿茶酚胺和芳香胺。考察了电动进样时间、分离电压以及毛细管出口与电极的间距对响应电流和分离效能的影响。通过对酚和儿茶酚胺测定的重现性、浓度线性范围及检出限的考察，结果表明该安培池是精密可靠的。同时，用该安培池对标准加入于血浆样品中的多巴胺（ＤＡ）、去甲基肾上腺素（ＮＥ）和原儿茶醛（ＰＡ）进行了测定，结果令人满意。     

毛细管区带电泳电化学检测器

本文阐述了毛细管区带电泳探针式安培检测器的构造、工作原理、理论及其与电泳系统的联结技术;介绍了该检测器的应用情况。     

Assay of Histamine in Single Mast Cells by Capillary Zone Electrophoresis with Electrochemical Detection

Histamine is one of the chemical mediators in connection with allergies. Only Pihel et al.1 reported a method for detection of histamine in isolated mast cells by high- performance liquid chromatography with electrochemical detection. In this method, single cells were removed and transferred to 300-nL microvials. A part of the supernatant from individual vials was injected into the chromatography column. The analysis of histamine in individual rat peritoneal mast cells using CZE with amp…     

高效毛细管电泳安培检测的进展

本文对高效毛细管电泳电化学检测方法中的安培检测进行了评述，安培检测具有灵敏度高，选择的特点，安培检测根据毛细管内径的大小有离柱安培检测和柱端安培检测，近年来脉冲安培分析法、化学修饰电极也已被引入毛细管电泳电化学检测。     

高效毛细管电泳—电化学检测对废水中优先监测酚类的测定研究

高效毛细管电泳－电化学检测法对６种中国环境优先监测的酚类进行了测定研究，考察了各实验参数对分离、检测的影响。在选定的最佳实验条件下，２５ ｍｉｎ内基线分离了上述 ６种酚类。测得浓度检测限（ｍｇ／Ｌ）：苯酚 ０． ０１；间－甲酚 ０．０１；２，４－二氯酚 ０．０６； ２，４，６－三氯酚０．０８；五氯酚０．１２；对硝基酚０．０７。本方法对实际废水样品中酚的测定结果满意。     

A New Conductivity Detector for Capillary Electrophoresis

IntroducnonHighperformancecapillaryelectrophoresis(HPCE)isanewanalyticaltechniqueandhasbeenappliedinvariousfields.InthedetectionmodesforHPCE,conductivitydetectionisaratheruniversalone,includingon-column',end-columnZandsuppressedconductivity'detection.Inthefirsttwo,thereisayieldedpotentialbetweentwoconductiveelectrodes,whichisinterferedbytheseparationvoltage.Theyieldedpotentialmayeasilydestroythegeneralconductivitymeter.ForthedetectorwithsuppressoF,itisdifficultinmanufactureanditsdeadvolum…     

A New Method for Decreasing Mass Limit of Detection and Increasing Number of Theoretical Plates in Capillary Electrophoresis with Amperometric Detection

Much attention has been focused recently on pushing down the limit of detection (LOD)1-3. LOD in terms of amount is a combination of concentration LOD and the volume of sample required. The detection of small amount of sample can be achieved by reducing the sample volume, lowering the sample concentration LOD or a combination. Sampling with capillary can reduce sample volume to nL-pL level. To lower the concentration LOD is related to improve the signal-to-noise ratio. In the prese…     

毛细管区带电泳碳纤维电极电化学检测氯丙嗪

用毛细管区带电泳／电化学检测器检了氯丙嗪。在最佳实验条件下，测定氯丙嗪的线性范围为５＊１０＾－７－１＊１０＾－４ｍｏｌ／Ｌ；检测限为１＊１０＾－７ｍｏｌ／Ｌ。此方法已应用于模拟尿样中氯丙嗪的测定。     

Determination of Active Ingredients of Origanum Vulgare L. and Its Medicinal Preparations by Capillary Electrophoresis with Electrochemical Detection

Abstract

A method based on capillary electrophoresis with electrochemical detection (CE‐ED) has been developed for the first time for the separation and determination of isovanillic acid, vanillic acid, quercetin, rosmarinic acid, caffeic acid, and protocatechuic acid in Origanum vulgare L. and its medicinal preparations. The effects of working electrode potential, pH level, concentration of running buffer, separation voltage, and injection time on CE‐ED were investigated. Under the optimum conditions, the analytes could be separated in a 50 mmol L^?1 borate buffer (pH 8.7) within 21 min. A 300‐µm diameter carbon disk electrode has a good response at +0.95 V (vs. SCE) for all analytes. The response was linear over three orders of magnitude with detection limits (S/N=3) ranging from 4×10^?8 g mL^?1 to 2×10^?7 g mL^?1 for the analytes. The method has been successfully applied to the analysis of real sample, with satisfactory results.     

Determination of Amino Acids in Single Human Lymphocytes after On-capillary Derivatization by Capillary Zone Electrophoresis with Electrochemical Detection

Sensitive and selective methods for the detection of amino acids (AAs) in single cells are of increasing importance. Capillary zone electrophoresis (CZE) with electrochemical detection (ED) is suitable for analysis of electroactive contents in single cells. However, most native AAs have no electroactivity. Derivatization with an electroactive tag is an attractive method. An on-capillary derivatization scheme was reported1. Naphtha- lene-2, 3-dicarboxaldehyde (NDA) can react with pri…     

毛细管电泳-柱端电化学检测池的设计

在射壁式检测器的基规上,设计了一种用于毛细管电泳-电化学检测装置的检测池,巧妙地利用因表面张力现象而留驻波滴曲面的放大作用,直接调整毛细管与电极之间的相对位置,无需显微镜-微调节器装置,具有空间体积小,操作更简便的优点。     

毛细管电泳电化学检测单糖的研究

采用毛细管区带电泳直立式安培电化学检测方法分离和测定单糖。考察了电解质溶液的ＰＨ和浓度对单糖分离的影响，适量加入氯化钠可改善分离条件。应用该法进行了乳糖的组成和人血中葡萄糖的测定。     

毛细管电泳-电化学检测法测定蜘蛛香中多元酚类化合物

采用毛细管电泳 电化学检测法(CE ED)同时测定了蜘蛛香根中香叶木素、山奈酚、芹菜素、绿原酸 和咖啡酸等5种主要生物活性成分的含量,考察了运行缓冲液酸度、浓度、分离电压、氧化电位和进样时间等 实验参数对分离、检测的影响。在最佳实验条件下,以直径300μm的碳圆盘电极为工作电极,检测电位为+ 950mV(vs.SCE),在50mmol/L的硼砂缓冲溶液(pH9.23)中,上述各组分在23min内能完全分离。5种组 分在两个数量级的范围内呈良好线性关系,检测下限(S/N=3)达1.7×10-4～1.8×10-5g/mL。该法已成功 地应用于蜘蛛香根中活性成分的分离检测,结果令人满意。     

毛细管电泳电导法快速检测饲料添加剂氯化胆碱

采用毛细管电泳电导法对饲料添加剂氯化胆碱进行了测定.实验优化了缓冲介质、分离电压、毛细管长度和内径等参数.结果表明:在优化条件下,氯化胆碱在4 min左右可以实现分离检测,线性范围在41.8～1.20 μg/mL,检出限为0.8 μg/mL;迁移时间和峰面积的相对标准偏差分别为0.6%、2.1%;样品加标回收率为97%～105%.将该法用于粉剂和饲料中氯化胆碱的检测,取得了满意的结果.     

A New Amperometric Detector Cell for Capillary Zone Electrophoresis

IntroductionSincethefirstamperometricelectrochemicaldetection(AED)forcapillaryelectrophoresis(CE)wasreportedbyWallingfordandEwing'inl987,AEDtechniquehasemergedasoneofthemostpowerfuldetectionmethodsforCEowingtoitsselectivity,sensitivity,lowcostanditsabilitytodetectlowlevelsofmanybioIogicallyrelevantmoleculeswithoutpriorderivatization2.However,twoproblemsassociatedwiththecombinationofAEDandCEhavehinderedthewidespreaduseofCE-AEDinroutineanalysis.Oneproblemistheinterferenceofhighvoltage(u…     

高效毛细管电泳中的激光光热检测技术

集中介绍了激光光热分析在高效毛细管电泳检测上的应用；分析了各种光热检测构型的特点，阐述了影响光热信号的各种因素；指出了激光光热检测技术在高效毛细管电泳中的应用前景。