左氧氟沙星-CdS-BSA体系荧光猝灭法测定左氧氟沙星

以六偏磷酸钠为稳定荆,巯基乙酸为修饰剂,水相合成了具有优异光学性质的CdS量子点.利用左氧氟沙星对CdS与牛血清白蛋白复合物的荧光有明显的猝灭作用,在536nm测定其荧光强度.结果表明,体系荧光猝灭程度与左氧氟沙星的浓度在0.2～20μg/mL范围内呈良好的线性关系,r=0.9994,检出限为0.0289μg/mL.方法已用于盐酸左氧氟沙星片剂和胶囊的测定,并与药典方法一致.     

左氧氟沙星-Al~(3+)-十二烷基硫酸钠体系胶束体系分光光度法测定左氧氟沙星

在B-R缓冲介质中,十二烷基硫酸钠(SDS)对左氧氟沙星(LVFX)-Al3+体系有显著的增敏作用,以此为基础建立了利用SDS增敏分光光度法测定LVFX的新方法。研究表明,体系吸光度与LVFX浓度在0.2~10μg/mL范围内呈良好的线性关系,相关系数r=0.9993,检出限为0.096μg/mL。将该方法用于盐酸左氧氟沙星片剂的测定,结果满意。     

刚果红-孔雀石绿-左氧氟沙星分光光度法测定左氧氟沙星的含量

在pH=3.55的NaAc-HAc缓冲溶液中,左氧氟沙星对刚果红(CR)和孔雀石绿(MG)反应生成的产物在可见光区有明显的作用,在618 nm波长处测定其吸收强度,结果表明体系的吸光度与左氧氟沙星的浓度在2～60μg.mL-1范围内呈现良好的线性关系,线性相关系数R=0.9905,检出限为0.053μg.mL-1。该方法可用于盐酸左氧氟沙星片剂、胶囊以及尿液中左氧氟沙星含量的测定。     

胶束增敏荧光法测定盐酸沙拉沙星

在pH 5.3的乙酸-乙酸钠缓冲溶液中,十二烷基苯磺酸钠能够敏化盐酸沙拉沙星的荧光,使λex=335 nnq、λem=450 nm的荧光增强.盐酸沙拉沙星质量浓度在0.10～6.99 mg/L范围内与荧光强度呈线性关系,方法检出限为9.16μg/L.6次平行测定方法回收率为91.8%～107.2%,相对标准偏差为1.6%～3.7%.常见金属离子及药物敷料对测定无干扰,不经分离直接用于兽药中盐酸沙拉沙星的测定.     

氧氟沙星在胶束体系中的荧光特性及应用

研究发现十二烷基硫酸钠胶束对氧氟沙星荧光有明显的增敏作用，据此建立了直接测定人体尿液中氧氟沙星的等波长差同步荧光光谱法（△λ=90nm）。经样品测定，其线性范围为0．12－3．6mg/L，检出限为0．12mg/L，回收率为92．2％－97．8％，相对标准偏差为1．2％－2．7％。     

替硝唑联合盐酸左氧氟沙星对慢性盆腔炎患者的临床治疗价值

目的研究分析替硝唑联合盐酸左氧氟沙星对慢性盆腔炎患者的临床治疗价值。方法选取2015年9月至2016年9月期间在江西省共青城市人民医院治疗的90例慢性盆腔炎患者作为研究对象,采用随机数字表法将90例患者分为观察组和对照组,每组各为45例,评价两组治疗效果,评价指标包括治疗有效率和不良反应发生率。结果观察组患者治疗有效率比对照组高,两组数据比较差异有统计学意义(P0.05);观察组患者不良反应发生率比对照组低,两组数据比较差异有统计学意义(P0.05)。结论替硝唑联合盐酸左氧氟沙星对慢性盆腔炎患者的临床治疗价值较高,值得推荐采纳。     

Ru(bpy)_3~(2+)体系电化学发光法测定盐酸左氧氟沙星

基于盐酸左氧氟沙星对联吡啶钌(Ru(bpy)2+3)的电化学发光信号有较强的增敏作用,建立了一种检测盐酸左氧氟沙星的电化学发光分析新方法。结果表明,在0.1mol/L NaHPO4-KH2PO4介质中,以恒电位电解,Ru(bpy)2+3的浓度为1.0×10-5g/mL时,盐酸左氧氟沙星对Ru(bpy)2+3电化学发光信号的增敏效果最好。在此条件下,测定盐酸左氧氟沙星的线性范围为6~400×10-9g/mL,检出限为5.0×10-9g/mL,对8.0×10-9 g/mL盐酸左氟沙星平行测次11次,相对标准偏差(RSD)为1.9%。该方法已应用于人体尿样中盐酸左氧氟沙星的测定,并且用此方法对盐酸左氧氟沙星的国产及进口片剂进行了体外溶出度的测定。     

胶束增敏荧光法测定盐酸二氟沙星的含量

基于十六烷基三甲基溴化铵可明显增强盐酸二氟沙星的荧光强度,建立了胶束增敏荧光法测定盐酸二氟沙星含量的方法。结果表明盐酸二氟沙星浓度在2～10000 ng·mL^-1范围内,荧光强度与药物的浓度成线性关系,线性方程为F=0.85C+313.29,相关系数R=0.9920,检出限为0.074 ng·mL^-1,回收率在96.10%～109.40%之间。该方法灵敏度高、操作简便、准确度高,用于盐酸二氟沙星注射液含量和肉类食品中盐酸二氟沙星残留的测定,所得结果可靠。     

胶束增敏荧光光谱法测定恩诺沙星的含量

研究了恩诺沙星(EFLX)在胶束体系中的荧光特性,发现十二烷基硫酸钠(SDS)对EFLX有较强的增敏作用,据此建立了胶束增敏荧光光谱法测定恩诺沙星的新方法。方法的线性范围为0-0.64μg/mL,检出限为4.75 ng/mL。     

胶束增敏荧光光度法测定小檗碱的含量

在pH 10的磷酸盐缓冲介质中,十二烷基苯磺酸钠(SDBS)对小檗碱的荧光有明显的增敏作用,且其荧光的增强程度与小檗碱浓度在2.0×10-9～1.0×10-3mol·L-1之间呈线性关系.此反应对小檗碱的检出限(3S/N)为1.29×10-9mol·L-1.利用此反应作为测定小檗碱的方法并应用于小檗碱片剂的分析,测得结果与药典法测定结果相符,对药片的标示量而言,测定所得回收率的平均值为99.8%,测定结果的相对标准偏差(n=5)的平均值为0.17%.     

衍生荧光法测定盐酸西布曲明

通过衍生反应，建立了用荧光光度法测定盐酸西布曲明的新方法。并对衍生体系、反应条件、产物稳定性等进行了研究。实验确定丙二酸和乙酸酐混合试剂与盐酸西布曲明在75℃的水浴中加热15min，所得产物发射强荧光，最大激发波长λcx=456nm，最大发射波长λem=496nm。在上述波长下，荧光强度与盐酸西布曲明的质量浓度在0．11～9．5μg／mL范围内呈良好的线性关系，检出限为13．4ng／mL，标准加入回收率在95．4％～104．2％。用于曲美胶囊中盐酸西布曲明的测定。     

胶束增敏荧光光谱法测定吡哌酸

采用不同表面活性剂对吡哌酸在胶束体系中的荧光性质进行了研究，发现在酸性介质中十二烷基硫酸钠(SDS)对吡哌酸有较强的增敏作用，据此建立了胶束增敏荧光光谱法测定吡哌酸的新方法。方法线性范围为0．07-0．36μg／mL，检出限为0.04μg／mL，平均回收率为98．6％-101．3％，相对标准偏差为1．1％-1．2％。样品测定结果令人满意。     

十二烷基磺酸钠增敏电化学法超灵敏地测定尿液中拓扑替康盐酸盐

提出了在一次性电极(改进的石墨浸蜡电极)上,利用十二烷基磺酸钠增敏电化学法亚皮摩尔级测定拓扑替康盐酸盐的方法.考察了不同种类的表面活性剂对拓扑替康盐酸盐的电化学响应.结果表明,十二烷基磺酸钠能够显著地提高其电化学响应.在优化的实验条件下,氧化峰电流跟拓扑替康盐酸盐浓度的对数在2.0×10-12至1.0×10-11mol/L和8.0×10-11至8.0×10-10mol/L范围内成线性关系,检出限为6.4×10-13mol/L.该方法用于检测尿液中的拓扑替康盐酸盐.     

A viscometric study of tuning micellar morphology by organic additives

The micellar morphology in aqueous 0.2 M sodium dodecyl sulfate (SDS) solutions has been studied in the simultaneous presence of organic salts (anilinium hydrochloride, AHC; ortho-toluidine hydrochloride, oTHC; para-toluidine hydrochloride, pTHC) and aliphatic alcohols (n-butanol, C₄OH; n-pentanol, C₅OH; n-hexanol, C₆OH; n-heptanol, C₇OH), aliphatic amines (n-butylamine, C₄NH₂; n-pentylamine, C₅NH₂; n-hexylamine, C₆NH₂; n-heptylamine, C₇NH₂), or hydrocarbons (n-hexane, C₆H; n-heptane, C₇H) by viscosity measurements under Newtonian flow conditions at 30 °C. Addition of alcohols and amines causes micellar growth which is found to be dependent upon chain length of the additive and nature of organic salt counterion. It is observed that amines are more effective in increasing the viscosity of the system if added in pure 0.2 M SDS solution, while SDS + pTHC system was found versatile when alcohols were added to this system. The increased viscosity or the micellar growth is explained in terms of the site of solubilization of the respective additive and the interaction of the additive with micellar surface and salt counterion present in the head group region. Hydrocarbons are nearly ineffective in inducing micellar growth and can be used as ‘micellar destroyer’ for the grown micelles. The additives used are found effective in tuning the environment of the micelle which is reflected in viscosity behavior. This work may find use in micellar ultrafiltration as well as in mimicking the natural cell, which has several aspects common with the micelle.     

Sodium dodecyl sulfate sensitized electrochemical method for sub-picomole level determination of topotecan hydrochloride at a novel disposable electrode

A sub-picomole level topotecan hydrochloride determination method was first proposed using sodium dodecyl sulfate (SDS) as a sensitized reagent at a novel disposable electrode (an improved wax-impregnated graphite electrode).The effects of different kinds of surfactants on the electrochemical response to topotecan hydrochloride were examined.The results indicate the electrochemical signal was apparently improved by SDS.At optimal conditions,the oxidative peak current increased linearly with the logarithm of...     

稳态荧光探针法测定临界胶束聚集数

以芘为荧光探针、二苯甲酮为猝灭剂,用稳态荧光探针法测定了SDS和AS的胶束聚集数(Nm).以芘的饱和水溶液为溶剂配制表面活性剂溶液,二苯甲酮的适宜浓度取小于1.00 mmol•L－1时,可以获得满意的实验结果.当表面活性剂溶液浓度为5～9倍cmc时,Nm随表面活性剂浓度增大而线性增大,而随温度的变化略有波动.cSAA=cmc时,Nm值为一本征值,定义为临界胶束聚集数[Nm].[Nm]值可从Nm－cSAA实验曲线外延得到.25℃时SDS的临界胶束聚集数[Nm]为57;40℃时SDS的[Nm]为49,AS的[Nm]为55.     

利尿剂的胶束色谱分析

用十二烷基硫酸钠（SDS）胶束溶液作为流动相，建立了11种利尿剂的胶束色谱系统分析方法；研究了SDS浓度、流动相的pH、有机改性剂及温度对色谱保留行为的影响，优化了色谱条件，并且不经化学处理直接进样测定了人尿中的烯睾丙内酯；该法适用于兴奋剂中利尿剂的常规分析。     

Rheology of viscoelastic anionic micellar solutions in the presence of a multivalent counterions

The growth and structure of anionic micelles sodium dodecyl trioxyethylene sulfate (SDES) in the presence of a multivalent counter-ion, Al³⁺, were investigated by means of rheological methods and the technique of freeze-fracture transmission electron microscopy. It was found that wormlike micelles and network structures could be formed in SDES/AlCl₃ aqueous micellar solutions, according to the measurements of the zero-shear viscosity, the complex viscosity and the dynamic moduli (storage modulus and loss modulus), and the application of the Cox–Merz rule and a Cole–Cole plot. The cyclic shear test, the plateau modulus and the relaxation time were also studied to express the rheological properties of the wormlike micellar solutions. The structure was of a character of a nonlinear viscoelastic fluid and departed from the simple Maxwell model. Received: 23 November/2000 Accepted: 31 January 2001