共查询到19条相似文献,搜索用时 62 毫秒
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随着生物无机化学的发展,配位体交换色谱用于生物体小分子的分离,形成了色谱化学中新兴而注目的研究领域。许多金属配位体络合物是具有手性的,当这种配合物同其它的光学活性配位体发生配位体交换反应时,往往显 相似文献
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手性配体交换色谱法直接拆分DL-氨基酸 总被引:2,自引:0,他引:2
手性配体交换色谱法在多种色谱拆分技术中占有重要地位。首先,手性配体交换色谱法往往具有较大的拆分因子,可进行半微量制备性拆分;其次,该技术可直接拆分氨基酸、羟基酸等极性化合物,不需要柱前衍生化。因此,自1970年Davankov及其同事首次用配体交换色谱拆分DL-脯氨酸以来,该技术受到人们的普遍重视。在七十年代,有关的研究报道几乎占色谱拆 相似文献
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手性配位体交换流动相添加剂法拆分对映体 总被引:7,自引:0,他引:7
综述了手性配合基交换色谱法通常采用三种手性相系统中的流动相添加剂方法。主要内容有:(A)手性配合基交换机制,给出了描述对映体对在色谱系统中的保留时间和分离选择性的公式,包括手性选择剂在固定相和流动相中的各种不同情况。公式表明整个色谱往系统的对映体选择性不同于溶液中所存在的选择剂与被选择物作用的情况;(B)影响配合交换的参数,讨论了金属离子、金属离子/配位体比率、金属离子络合物浓度、固定相、流动相pH、洗脱顺序、有机调节剂、离子对试剂、流动相离子强度、温度、立体选择性和手性交互识别;(C)应用。 相似文献
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采用配位体交换树脂对中性氨基酸(Gly,Ala,Val和Leu)进行了分离,并与一般阳离子交换树脂的分离性能进行了比较,对淋洗剂、柱温等分离条件进行了实验;研究了树脂载体结构、功能基及配位中心离子对分离性能的影响。 相似文献
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对映体选择性配体交换膜的制备和DL-氨基酸的拆分 总被引:8,自引:0,他引:8
制备了一种新型光学分离膜,即带有L-脯氨酸手性选择子的交联聚乙烯醇膜,考察了DL-酪氨酸,DL-苯丙氨酸和DL-色氨酸通过膜的对映体选择性渗透性能。发现L-氨基酸优先透过膜,消旋氨基酸透过膜的对映体拆分机理类似于手性配体交换色谱方法拆分消旋氨基酸的机理,在DL-氨基酸的对映体选择性膜透过中,对映体选择性吸附着重要的作用。 相似文献
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亲水性手性配体树脂对氨基酸的拆分 总被引:4,自引:0,他引:4
本文设计并合成了新型亲水性手性配体铜络合聚乙烯胺树脂,并以此树脂为高效液相色谱固定相,对一系列氨基酸进行了拆分研究,对拆分机理进行了探讨,并提出了合理的模型解释。 相似文献
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Four neutral amino acids (Gly, Ala,.Val and Leu) were separated with ligand exchange resins. The separation capacity of the ligand exchange resins is compared with that of common ion exchange resins. The effects of eluent, column temperature, and central metal ions of the support on the separation are studied. The relationship between matrix structure of resins and their separation capacity is analysed. 相似文献
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《液相色谱法及相关技术杂志》2012,35(4):701-712
Abstract A method for the direct separation of racemates by HPLC is described. A chiral stationary phase is synthesized, suitable for ligand exchange chromatography. L-proline is chemically bonded to silica gel via 3-glycidoxypropyltrimethoxysilane. The bonded support is loaded with Cu(II) ions as a complexing agent. Complete resolution of amino acid racemates can be obtained in less than ten minutes. α-values up to 3.5 are observed. 相似文献
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《液相色谱法及相关技术杂志》2012,35(4):501-513
Abstract Using novel class chiral ligand polymers which have different chemical environment in chiral cavity, we investigated their functional properties of chiral recognition for DL-amino acids and discussed ligand exchange chromatography of DL-amino acids on different chromatographic conditions in detail. 相似文献
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The paper discribes the resolution of dl-Phe, dl-Phser, dl-Tyr, dl-Tryp, dl-His etc. by ligand exchange chromatography using L-Pro grafted resins coordinated with copper (Ⅱ) as stationary phase. Except dl-His, the other amino acids mentioned above could be completely resolved and D-amino acids were eluated before L-amino acids. Through determination of IK spectra of L-Pro-PGMA-Cu resin, its possible coordinated model of structure of resin and mechanism of resolution are presented. 相似文献
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《Electroanalysis》2004,16(11):955-960
Enantioselective resolution is realized by combining potentiometry with ligand exchange (CE) in a new method called chiral ligand exchange potentiometry (CLEP). A chiral selector, N‐carbobenzoxy‐L ‐aspartic acid (N‐CBZ‐L‐Asp), preferentially recognizes D ‐aspartic acid (D‐Asp) and undergoes ligand exchange with the enantiomeric labile coordination complexes of [Cu(II)(D‐Asp)2] or [Cu(II)(L‐Asp)2] to form a diastereoisomeric complex [(D‐Asp)Cu(II)(N‐CBZ‐L‐Asp)] (a) or [(L‐Asp)Cu(II)(N‐CBZ‐L‐Asp)] (b). Considerable stereoselectivity occurs in the formation of these diastereoisomeric complexes, and their net charges were ?2 (a) and 0 (b), respectively, resulting in different Nernst factor (electrode slope), thus enabling chiral D‐Asp to be distinguished by potentiometry without any pre‐ or postseparation processes. 相似文献
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Yuanqi Gui Baian Ji Gaoyi Yi Xiuju Li Kailian Zhang Qifeng Fu 《Molecules (Basel, Switzerland)》2021,26(6)
Herein, a novel L-arginine (L-Arg)-modified polydopamine (PDA)-coated capillary (PDA/L-Arg@capillary) was firstly fabricated via the basic amino-acid-induced PDA co-deposition strategy and employed to constitute a new chiral ligand exchange capillary electrochromatography (CLE-CEC) method for the high-performance enantioseparation of D,L-amino acids (D,L-AAs) with L-Arg as the immobilized chiral ligand coordinating with the central metal ion Zn(II) as running buffer. Assisted by hydrothermal treatment, the robust immobilization of L-Arg on the capillary inner wall could be facilely achieved within 1 h, prominently improving the synthesis efficiency and simplifying the preparation procedure. The successful preparation of PDA/L-Arg coatings in the capillary was systematically characterized and confirmed using several methods. In comparison with bare and PDA-functionalized capillaries, the enantioseparation capability of the presented CLE-CEC system was significantly enhanced. Eight D,L-AAs were completely separated and three pairs were partially separated under the optimal conditions. The prepared PDA/L-Arg@capillary showed good repeatability and stability. The potential mechanism of the greatly enhanced enantioseparation performance obtained by PDA/L-Arg@capillary was also explored. Moreover, the proposed method was further utilized for studying the enzyme kinetics of L-glutamic dehydrogenase, exhibiting its promising prospects in enzyme assays and other related applications. 相似文献