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1引言环糊精及其电中性衍生物(二甲基-β-环糊精(DM-β-CD)、三甲基-β-环糊精(TM-β-CD)、羟丙基-β-环糊精(HP-β-CD))是毛细管电泳(HPCE)手性分离中最常采用的手性选择剂,带电环糊精是HPCE手性分离中另一类重要的手性选择剂,它的带电性质使其在手性拆分过程中起到非常重要的作用。本文分别考察了带电的磺丁基-β-环糊精(SBB-β-CD)与电中性的DM-β-CD、TM-β-CD以及HP-β-CD等3种双环糊精体系的手性拆分性能,研究了双环糊精在手性分离中所体现出的协同效应… 相似文献
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羧甲基—β—环糊精用于毛细管电泳中的手性分离 总被引:4,自引:0,他引:4
本文将水溶性良好的羧甲基-β-CD(CM-β-CD_用于分离氯霉素中间体,考察了CM-β-CD作为毛细管电泳手性选择剂的分离性能。 相似文献
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以β—环糊精及其衍生物为手性选择性毛细管电泳拆分几种药物的旋光… 总被引:6,自引:0,他引:6
在毛细管电泳中,采用β-环糊精(β-CD)及其衍生物为手性选择剂对扑尔敏、异丙嗪、二氧异丙嗪和氧氟沙星对映体进行了分离。优化了分离条件;讨论了环糊精空腔边缘与溶质分子作用的基团的种类和分离效果的联系,通过计算手性分离的热力学常数比较了不同手性选择剂对扑尔敏对映体的分离能力。最后,讨论了乙腈在手性分离中的作用。 相似文献
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毛细管电泳是一种分离手性化合物极为有效的工具。本文用β-环糊精作为手性选择剂在CZE的模式下对取代四氢β-咔啉类手性化合物进行了成功的拆分。 相似文献
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A novel water-soluble beta-CD-derivatized erythromycin (EM) was synthesized and used as an effective chiral selector for the resolution of chiral compounds in CZE. The purpose of substitution at the primary hydroxyl site of beta-CD with 1-oxygen-2,3-epoxypropane is to produce a compound having functions of both beta-CD and EM. beta-CD-derivatized EM exhibited excellent enantioselectivities compared with single beta-CD and EM for chiral separation in CE. We also investigated the influence of pH and concentration of BGE, concentration of chiral selector, applied potential, and organic modifier on chiral compounds' separation. 相似文献
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建立了毛细管电泳手性分离多沙唑嗪中间体对映体的方法,并同时分离了多沙唑嗪对映体。考察了不同种类季铵盐对电渗流及分离的影响,其中四甲基氢氧化铵(TMB)能有效控制电渗流并提高组分的分离度。实验还考察了其他因素,如pH值、分离电压和磷酸二氢钠浓度对分离的影响。所用的毛细管为40 cm(有效长度30 cm)×50 μm,缓冲液为12 mmol/L β-环糊精、30 mmol/L TMB、60 mmol/L 磷酸二氢钠(pH 2.2),分离电压为20 kV。在此条件下多沙唑嗪及其中间体的对映体均达到了基线分离。实验结果表明,一些用β-环糊精不能完全分离的对映体通过加入TMB控制电渗流能达到满意的分离效果。 相似文献
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Electrokinetic chromatography (EKC) was employed to achieve the enantiomeric separation of a group of chiral 1,4-dihydropyridines (DHPs) with pharmacological activity. Micelles of bile salts alone or mixed with neutral cyclodextrins, micelles of sodium dodecyl sulfate (SDS) mixed with neutral cyclodextrins, and anionic cyclodextrin derivatives, i.e., carboxymethyl-gamma-cyclodextrin (CM-gamma-CD), carboxymethyl-beta-cyclodextrin (CM-beta-CD), and succinylated beta-cyclodextrin (Succ-beta-CD), were employed as pseudostationary phases. The enantiomeric separation ability of these chiral selectors with respect to DHPs was studied in different experimental conditions. CM-beta-CD was shown to be the best chiral selector to perform the enantiomeric separation of DHPs by EKC. Next, the influence of the CM-beta-CD concentration, the pH and nature of the buffer, the temperature, and the applied voltage on the enantiomeric resolution of DHPs was studied. The use of a 50 mM ammonium acetate buffer, pH 6.7, 25 mM in CM-beta-CD together with an applied voltage of 15 or 20 kV, and a temperature of 15 degrees C enabled the individual enantiomeric separation of twelve DHPs, each one into its two enantiomers, and their separation in multicomponent mixtures of up to six DHPs into all their enantiomers. 相似文献
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The chiral separation of simendan enantiomers using capillary electrophoresis was studied with beta-cyclodextrin (beta-CD) as chiral selector. The influences of the concentration and pH of borate buffer solution, beta-CD concentration and methanol content in the background electrolyte were investigated. These factors were compared with those in an HPLC with beta-CD as chiral mobile phase additive (CMPA-HPLC). The quantification properties of the developed CE method were examined. A baseline separation of simendan enantiomers was achieved in the background electrolyte of 20 mmol/L borate buffer (pH 11.0) containing 12 mmol/L beta-CD-methanol (50:50 in volume ratio). The CE method is comparable with CMPA-HPLC in chiral resolution, although the optimal pH in CE (11.0) is much higher than that (6.0) in CMPA-HPLC. This chiral CE method is applicable to the quantitative ananlysis and enantiomeric excess value determination of L-simendan. 相似文献
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Au nanoparticlesAuNPswere synthesized by chemical reduction methodand its surface was chiral modified with serine to prepare Ser-AuNPs. The synthesis effect and particle size distribution of AuNPs and Ser-AuNPs were characterized by UV-Vis spectrophotometry and laser particle size analyzer. The Ser-AuNPs was used as a chiral selector for the enantiomeric separation of three adrenaline drugs by capillary zone electrophoresis. The influences of the binding concentration of serinebuffer pHseparation voltage and capillary temperature on the enantioseparation were investigated. The results showed that the enantiomeric separation of isoprenaline adrenaline and noradrenaline could achieve baseline separation at the optimal separation conditions. The corresponding resolutions were 3.192.85 and 3.77 for isoprenalineadrenaline and noradrenalinerespectively. And this method possessed good reproducibility and precision. © 2022, Youke Publishing Co.,Ltd. All rights reserved. 相似文献
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A capillary zone electrophoresis (CZE) method with direct ultraviolet (UV)-absorbance detection is presented for the simultaneous enantiomeric separation of warfarin and its main metabolites, including warfarin alcohols, 4'-, 6-, and 7-hydroxywarfarin, using highly sulfated beta-cyclodextrin (HS-beta-CD) as the chiral selector. This chiral separation method was optimized in terms of the electrophoretic parameters, which included the concentration of HS-beta-CD used, the type and composition of organic modifier added to the background electrolyte (BGE) buffer, and the BGE buffer pH. Chiral separation of warfarin and its major metabolites was achieved with high resolution, selectivity, efficiency, repeatability, and reproducibility. This optimized chiral analysis of warfarin along with its metabolites was completed within a satisfactory electrophoresis time of 20 min. 相似文献
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A new capillary electrophoretic method was developed for enantiomeric separation and optical impurity analysis of three synthetic 2,3-dihydroxy-3-phenylpropionate compounds using native beta-cyclodextrin (beta-CD) as chiral selector and borate as a background electrolyte. The separation was carried out in uncoated capillary (58.5 cm x 75 microm I.D., effective length 48.5 cm). The results showed that beta-CD as the chiral selector exhibited good enantioselectivity and the baseline separation was obtained at pH 9.8, 200 mM borate buffer containing 1.7% beta-CD at applied voltage 15 kV and capillary temperature 20 degrees C within 15 min. The precision of each tested compound was less than 1.0% at migration time and 5.0% in corrected peak area and the accuracy of the method was in the range of 98.7-105%. Furthermore, the developed method was successfully applied to the determination of the undesirable trace (2S,3R)-(+)-form impurity in the synthetic (2R,3S)-(-)-2,3-dihydroxy-3-phenylpropionate samples. 相似文献
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Separations of basic drug enantiomers have been investigated using glucuronyl glucosyl beta-cyclodextrin (GUG beta-CD) as a chiral selector in the background electrolyte by capillary zone electrophoresis. The effects of GUG beta-CD concentration and running buffer pH on the migration times and resolution of 16 basic drug enantiomers were precisely examined using a linear polyacrylamide-coated capillary. High resolution of 16 basic drug enantiomers was generally attained with a running buffer pH 2.5 or 3.5 containing 10 mM GUG beta-CD. Next, we compared the chiral resolution abilities of GUG beta-CD with those of beta-CD and maltosyl beta-CD (G2 beta-CD). GUG beta-CD showed higher resolution for basic drug enantiomers tested than beta-CD and G2 beta-CD. This could be due to that hydrogen bonding or ionic interactions of uncharged and charged glucuronyl glucosyl groups of GUG beta-CD with an analyte could stabilize the inclusion complex. 相似文献