水中六价铬分析方法研究进展

对近年来水中六价铬的检测方法进行了综述,包括分光光度法、原子吸收分光光度法、离子色谱及其联用法、液相色谱及其联用法。对几种检测方法进行分析,展望了六价铬测定方法的发展方向。     

饮用水中有机污染物检测方法研究进展

介绍水中有机污染物的富集方法如顶空法、萃取法、固相微萃取法、液相微萃取法、棒吸附萃取等,对饮用水中有机污染物的检测技术如气相色谱法、气相色谱–质谱联用法、液相色谱法、液相色谱–质联用法等进行了综述并对检测技术的发展趋势进行了展望。     

多维立体分离分析技术面临的挑战性问题及对应策略

高效液相色谱(HPLC)、毛细管电泳(CE)等高效分离技术的发展为蛋白质组、天然产物、环境样品等复杂体系的分离分析提供了新的手段。然而一维分离模式所能提供的分辨率和峰容量仍十分有限,难以满足人们对复杂样品进行高效分离与高灵敏检测的要求。多维分离是近年来发展起来的一种新型复合分离技术,与一维分离模式相比,这种技术可以极大地提高峰容量,便捷地调整分离选择性,因此已成为近期分析化学领域的重要研究热点。多维液相分离方法可以采用不同的HPLC模式(如排阻色谱、离子交换色谱、亲和色谱、反相色谱等)和CE模式(如毛细管区带…     

UltiMate 3000 DGLC双三元液相色谱的流动相在线除盐技术

近年来,LC-MS联用技术已被广泛应用于各个行业。液相色谱分离是联用技术的基础,然而现有的很多液相色谱分离方法为改善分离或检测经常会使用非挥发性缓冲盐流动相(如磷酸盐缓冲溶液或离子对试剂),这显然与质谱的ESI     

手性配体交换色谱的研究进展

手性配体交换色谱技术将配体交换原理与色谱技术相结合,拓宽了液相色谱、电泳以及逆流色谱的应用层面,为部分难分离手性物质提供了有效的分离与检测方法。该文参考了近年来国内外的相关文献,综述了手性配体交换技术在液相色谱、电泳与逆流色谱等领域中的应用进展;总结了手性配体交换色谱技术的基本原理、影响因素、优缺点等,并对手性配体交换色谱技术的趋势进行了展望。     

毛细管电色谱应用研究进展

对近年来毛细管电色谱(CEC)的研究现状以及实际应用进行归纳总结。以查阅国内外有关文献资料的方法,综述毛细管电色谱的研究现状以及在药物、食品、农残、化妆品、氨基酸以及环境检测等分析中的应用。毛细管电色谱在上述分析领域中获得了广泛的应用,同时对毛细管电色谱的未来发展方向(加压毛细管电色谱)进行了展望。毛细管电色谱拥有广阔的发展应用前景。     

三聚氰胺检测方法简述

三聚氰胺是一种三嗪类含氮杂环有机化合物,食用添加三聚氰胺的食品可导致结石生成,本文从气相色谱、液相色谱、电学化方法和可视化传感器四个方面综述了近年来三聚氰胺检测的若干方法,从实验方法、灵敏度、专一性三个角度对上述方法的优缺点进行对比,并对上述方法用于实际样品中三聚氰胺的检测进行可行性分析,提出了新的检测三聚氰胺的思路和方法。     

毛细管电色谱-激光诱导荧光联用技术进展

作为一种新兴的微分离技术,毛细管电色谱(CEC)结合了毛细管电泳的高效性和高效液相色谱高选择性的优点,近年来备受关注。本文简单介绍了CEC的基本原理、常用色谱柱和检测器。重点针对毛细管电色谱-激光诱导荧光检测联用技术进行了综述,包括该方法在生物、医药、环境等诸多领域的应用。     

咪唑键合硅胶固定相微柱液相色谱分离酚类和胺类化合物

由于微柱液相色谱(μ-LC)具有高检测灵敏度、低溶剂消耗、可以与质谱等多种检测器联用的优点,近年来受到广泛关注。将咪唑键合硅胶固定相填充到毛细管中,在自制的微柱液相色谱系统下利用此键合相具有的弱疏水作用,采用不同的流动相对酚类和胺类化合物进行了分离。结果表明,流动相中只需添加少量的有机溶剂就可以实现对一些有机化合物的分离,甚至可以只用纯水作流动相就能分离一些弱疏水性化合物,如酚类。微柱液相色谱的流动相用量少,避免或大大减少了对环境的污染。自制微柱液相色谱系统为下一步微柱液相色谱-质谱联用奠定了一定的基础。     

碳点在抗生素分析检测中的应用

抗生素的过度使用对环境造成了极大破坏,对其进行监测控制刻不容缓.常用的分析检测技术,如高效液相色谱(HPLC)、气相色谱(GC)、高效液相色谱-串联质谱(HPLC-MS/MS)等具有高效快速、重现性好、可自动化操作等优点.但对环境样品中抗生素的检测存在样品前处理过程繁琐、检测灵敏度低、实验成本高等问题.结合现有的检测技...     

食品农药残留分析进展

对食品中农药残留分析技术及其进展进行了综述。样品前处理中,除固相萃取外,超临界流体萃取和基质固相分散得到了飞速发展和广泛应用。原子激发检测器在气相色谱中发展较快,超临界流体色谱和免疫分析技术开发应用于食品农药残留分析中。并对农药残留分析的发展趋势和要求进行了讨论。     

高分辨质谱技术在农药残留检测中的应用

农药残留检测是农产品中有害物质控制的重要组成部分,随着农药残留限量标准体系的发展完善,农药残留检测方法也在不断进步。近年来质谱技术发展迅速,已被广泛应用于农药残留检测领域,高分辨质谱由于具有较高的分辨率和质量精确度,在复杂基质的农药多残留高通量检测中发挥着越来越重要的作用。本文从高分辨质谱与液相色谱、气相色谱及其他分离模式联用等方面出发,简述了近5年来高分辨质谱在农药残留检测中的应用,对目前高分辨质谱在农药残留检测应用中发现的问题进行了讨论,并对其未来发展趋势进行展望。     

Methods of sample preparation for determination of pesticide residues in food matrices by chromatography–mass spectrometry-based techniques: a review

Much progress has been made in pesticide analysis over the past decade, during which time hyphenated techniques involving highly efficient separation and sensitive detection have become the techniques of choice. Among these, methods based on chromatographic separation with mass spectrometric detection have resulted in greater likelihood of identification and are acknowledged to be extremely useful and authoritative methods for determination of pesticide residues. Even with such powerful instrumental techniques, however, the risk of interference increases with the complexity of the matrix studied, so sample preparation before instrumental analysis is still mandatory in many applications, for example food analysis. This article summarizes the analytical characteristics of the different methods of sample-preparation for determination of pesticide residues in a variety of food matrices, and surveys their recent applications in combination with chromatographic mass spectrometric analysis. We discuss the advantages and the disadvantages of the different methods, address instrumental aspects, and summarize conclusions and perspectives for the future.     

高效毛细管电泳在食品安全检测中的应用进展

近年来,食品安全问题频发,对人们的健康和社会发展造成了严重的危害,食品安全已成为人们关注的焦点问题之一。食品成分的复杂性、多样性对食品分析技术和方法提出了很高的要求。毛细管电泳(CE)由于分离模式多、分离效率高、分析速度快、试剂和样品用量少、对环境污染小等优点,在食品安全分析方面的应用日趋广泛。本综述对2009年以来CE在食品中非食用添加剂、农药残留、兽药残留、重金属离子污染、食品毒素以及食品包装材料中双酚A和塑化剂的检测方面的应用进行了总结,并对毛细管电泳在食品安全检测领域的主要发展方向进行了展望。共收录文献63篇。     

分子印迹聚合物在极性农药残留检测中的应用进展

极性农药包括杀菌剂、除草剂、杀虫剂等,种类丰富,成本低廉,在农业中应用广泛,其滥用易导致水资源和土壤等环境污染,人类通过间接接触动植物源性食品和环境中的极性农药残留也增加了农药暴露风险.极性农药的物理化学性质差异大,通常痕量存在于食品和环境样品等复杂基质中,这对其准确检测分析带来了挑战.分子印迹聚合物(MIPs)作为一...     

A review of sample preparation methods for the pesticide residue analysis in foods

The pesticide residues in foods have received increasing attention as one of the most important food safety issues. Therefore, more strict regulations on the maximum residue limits (MRLs) for pesticides in foods have been established in many countries and health organizations, based on the sensitive and reliable analysis methods of pesticide residues. However, the analysis of pesticide residues is a continuing challenge mainly because of the small quantities of analytes as well as the large amounts of interfering substances which can be co-extracted with them, often leading to experimental errors and damage to the analytical instruments. Thus, extensive sample preparation is often required for the pesticide residue analysis for the effective extraction of the analytes and removal of the interferences. This paper focuses on reviewing the recent development in the sample preparation methods for the pesticide residue analysis in foods since 2006. The methods include: liquid-liquid extraction (LLE), supercritical-fluid extraction (SFE), pressurized-liquid extraction (PLE), microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), gel permeation chromatography (GPC), solid-phase extraction (SPE), molecularly imprinted polymers (MIPs), matrix solid-phase dispersion (MSPD), solid-phase micro-extraction (SPME), QuEChERS, cloud point extraction (CPE) and liquid phase micro-extraction (LPME), etc. Particularly their advantages, disadvantages and future perspectives will be discussed.     

Sample preparation methods in the analysis of pesticide residues in baby food with subsequent chromatographic determination

Pesticides are widely utilized at various stages of cultivation and during postharvest storage to protect plants against a range of pests and/or to provide quality preservation. Reliable confirmatory methods are required to monitor pesticide residues in baby foods and to ensure the safety of baby food supply. This review covers methods in which pesticide residues have been determined in baby food by the use of a wide range of chromatographic techniques after various sample preparation steps. The main attention is paid to the evaluation and improvement of sample extraction and clean-up methods (liquid extraction, solid-phase extraction (SPE), dispersive SPE (DSPE), microextraction procedures, matrix solid-phase dispersion (MSPD) and supercritical fluid extraction (SFE)) considering low concentration levels of pesticide residues in baby food resulting from stringent European Union (EU) legislation. Instrumental aspects together with the matrix effects significantly contributing to the most important parameters considered in pesticide residues analysis of baby food--limits of detection (LODs) and limits of quantification (LOQs) were included within the scope of this overview. Paper involves also monitoring studies.     

Fast gas chromatography for pesticide residues analysis

The importance of method development in the area of pesticide residues analysis is apparent from legislative requirements continuously decreasing the maximum acceptable concentration levels in food and water. This covers also contribution in the science in the field of ultra-trace analysis of organic pollutants in complex mixtures. Analysis time is one of the most important aspects that should be considered in the choice of analytical methods for routine application. With this fact, fast gas chromatography (GC) has acquired a real importance in the pesticide residue analysis. This paper provides an overview of fast GC methods for analysis of pesticide residues in variety of matrices at ultra-trace concentration levels. Emphasis is put on the development in the last 6 years.     

Chromatographic methods for the determination of pesticides in foods.

Chromatography is the most important technique available to the analyst dealing with the determination of pesticide residues in food, feed and environmental samples. Numerous methods for pesticide residues in foods have been developed in the past few years, and this paper reviews some of the most important procedures. A great variety of chromatographic methods, such as solid-phase extractions, column chromatographic clean-up methods, thin-layer, gas, high-performance liquid and supercritical fluid chromatography, and their coupling with sensitive and selective detection methods are surveyed.     

气相色谱-负化学离子源质谱法在食品安全分析中的应用

气相色谱-负化学源质谱法(GC-NCI-MS)技术被广泛应用于环境、人体组织、食品等样品中污染物的定性分析和定量测定.综述了近年来气相色谱-负化学源质谱技术在食品安全分析如农药残留、兽药残留和污染物测定中的应用.