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《分析试验室》2021,40(5):605-612
DNA电化学生物传感器是一类以DNA为敏感元件或检测对象,将核酸分子特异性识别过程中产生的信号通过换能器转化为电信号,从而实现对目标物定性或定量检测的传感器,具有响应速度快、操作简单、选择性好、灵敏度高、检测成本低等优点,实现了多领域中重金属、真菌毒素、核酸等的快速实时检测。介绍了DNA电化学生物传感器的组装单元、电化学指示剂类型,以DNA二级构型角度综述了DNA电化学生物传感器的四大类特殊结构,并汇总其在临床、中医药、生态环境保护及食品安全等领域中重金属的检测应用研究,对新型DNA电化学生物传感器的设计与其在更多领域的拓展应用提供借鉴价值。 相似文献
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纳米电化学生物传感器 总被引:4,自引:0,他引:4
杨海朋|陈仕国|李春辉|陈东成|戈早川 《化学进展》2009,21(1):210-216
纳米电化学生物传感器是将纳米材料作为一种新型的生物传感介质,与特异性分子识别物质如酶、抗原/抗体、DNA等相结合,并以电化学信号为检测信号的分析器件。本文简要介绍了生物传感器的分类和纳米材料在电化学生物传感器中的应用及其优势,综述了近年来各类纳米电化学生物传感器在生物检测方面的研究进展,包括纳米颗粒生物传感器,纳米管、纳米棒、纳米纤维与纳米线生物传感器,以及纳米片与纳米阵列生物传感器等。 相似文献
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纳米尺度上的生物分析化学是当今国际生物分析领域研究的前沿和热点.该文阐述了纳米粒子在电化学免疫传感器及电化学DNA传感器领域的应用,着重介绍了以纳米材料为载体设计新型的具有生物分子识别和电信号增强作用的纳米标记粒子在构建高灵敏电化学生物传感器以及多组分同时检测中的应用. 相似文献
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石墨烯(Gr)是一类由单层碳原子组成的二维碳质材料,利用它独特的结构和良好的物理、化学性能,可构筑出在电催化、电化学传感器和生物传感器等领域有着巨大应用潜力的新型Gr功能复合材料。基于Gr功能复合材料的DNA电化学传感器与常规DNA传感器相比,具有明显的特色和优势,已被应用于特异DNA靶序列的识别和传感领域。本文就基于Gr功能复合材料的DNA电化学传感器的近期进展作简要评述,包括Gr与Gr基金属、金属氧化物、高分子、生物分子复合材料的电化学性能及其在DNA电化学传感中的应用,并对该类DNA电化学传感器的发展方向和应用前景进行了展望。 相似文献
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黄曲霉毒素电化学生物传感器 总被引:1,自引:0,他引:1
黄曲霉毒素(aflatoxin,AF)是一种具有强烈毒性和强致癌性的生物毒素,对其进行快速而准确的分析是减小和避免黄曲霉毒素危害的最有效手段之一。电化学生物传感器因其快速、灵敏、特异性强、易于微型化等优势在黄曲霉毒素分析受到国内外研究者的广泛关注。目前,应用于黄曲霉毒素分析的电化学生物传感器主要有免疫传感器、酶传感器和DNA传感器。本文综述了不同传感器的研究现状,特别介绍了新材料新技术在黄曲霉毒素免疫分析中发挥的重要作用,并对黄曲霉毒素的电化学生物传感分析存在的问题和发展前景进行了探讨及展望。 相似文献
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《Electroanalysis》2006,18(5):456-464
An electrochemical DNA biosensor was developed by DNA immobilization at the electrode surface and its electrochemical behavior was studied in relation with different materials added in the paste. The aim was to study new materials for the development of new electrode surfaces, to be applied in the study of DNA – drug interactions. New electrochemical sensing materials using polymer multilayers were reported for the adsorption of DNA. These materials were prepared by mixing a polymer ion exchanger and graphite powder. The mixture was then used to render the modified carbon paste electrode (CPE), on the surface of which the dsDNA was adsorbed and studied by differential pulse voltammetry (DP voltammetry). The signal of guanine oxidation peak of DNA was followed. This modified biosensor was applied for the study of the interaction between DNA and the known intercalators Ethidium Bromide (EB) and Acridine Orange (AO). The established biosensor exhibited an improvement of its sensitivity and repeatability compared with the conventional CPE DNA biosensor. 相似文献
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在研究DNA与儿茶酚胺类分子之间相互作用的基础上, 以碳纤维电极(CFE)为基底, 制备了一种新型的DNA-过氧化聚吡咯(PPyox)生物复合膜传感器, 与单一的DNA或PPyox修饰层相比具有更高的灵敏度和选择性. 相似文献
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Carlos H. S. Mendes Joo G. S. Montenegro Nathalia L. Queiroz Thayane C. S. Moreira Valberes B. Nascimento Severino Carlos B. Oliveira 《Electroanalysis》2020,32(1):19-28
This work presents a study of the electrochemical oxidation of 7‐methylguanine (7‐mGua) in aqueous solution at glassy carbon electrode by cyclic voltammetry, differential pulse voltammetry, square wave voltammetry and electrochemical impedance spectrometry. The anodic behaviour of 7‐mGua was compared with the electro‐oxidation of guanine and 7‐methylguanosine. The results demonstrated that the methyl and ribose groups are not electroactive but strongly influence the oxidation mechanism of these species. The oxidation of 7‐mGua occurred in a single pH‐dependent step, with the withdrawal of two electrons and two protons of C8, to form 8‐oxo‐7‐methylguanine, while the electro‐oxidation of 7‐methylguanosine also occurred in a single pH‐dependent step, however, with the withdrawal of one electron and one proton of C8 to form a hydroxylated product, since its oxidation to 8‐oxo‐7‐methylguanosine is hindered by the presence of the pendant groups. In addition, the oxidation of 7‐mGua was investigated in the presence of DNA and DNA‐bases, leading to the conclusion that the formation of 7‐mGua, from an interaction of DNA with an alkylating agent, would cause an increase on the deoxyguanosine peak current of the DNA‐biosensor, with no interference of any free DNA bases, which demonstrated that DNA‐electrochemical biosensors find application on detecting DNA methylation, opening a new avenue for applications of DNA biosensors. 相似文献
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《Analytical letters》2012,45(13):2309-2331
Abstract A report on the status of the electrochemical DNA probes for the detection of a DNA sequence for environmental and clinical studies is presented. The literature on the electrochemistry of DNA in the last ten years is reviewed. Results obtained in this laboratory using an electroactive hybridization indicator for constructing an electrochemical DNA probe are reported. 相似文献
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A novel electrochemical biosensor is described for detection of breakpoint cluster region gene and a cellular abl (BCR/ABL) fusion gene in chronic myelogenous leukemia (CML) by using thiolated-hairpin locked nucleic acids (LNA) as the capture probe. The hairpin LNA probe was immobilized on the nanogold (NG)/poly-eriochrome black T (EBT) film-modified glassy carbon electrode (GCE). The immobilized LNA probe could selectively hybridize with its target DNA on LNA/NG/EBT/GCE surface. The immobilization and hybridization of the LNA probe were characterized with cyclic voltammetry and electrochemical impedance spectroscopy. The hybridization of the immobilized LNA probe with the target DNA was detected by differential pulse voltammetry with the electroactive methylene blue as an indicator. The results indicated this new method has excellent specificity for single-base mismatch and complementary after hybridization, and a high sensitivity. This novel electrochemical biosensor has been used for assay of PCR real sample with satisfactory result. 相似文献
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The discovery of carbon nanotubes (CNTs) about a decade ago has brought fascinating evolutions in electronics, material industry,
as well as bio-techniques for DNA analysis, gene therapy, drug delivery etc. It has also dramatically promoted the development
of DNA biosensing techniques, especially electrochemical DNA biosensor. The application of CNTs in electrochemical DNA biosensors
includes two main aspects: on one hand, using CNTs as a novel substrate not only enables immobilization of DNA molecules but
also serves as a powerful amplifier to amplify signal transduction event of DNA hybridization. On the other hand, CNTs can
also be employed as a powerful carrier to pre-concentrate enzymes or electroactive molecules for electrochemical sensing of
DNA hybridization as a novel indicator. In this review, we place emphasis on recent studies of CNTs-based electrochemical
DNA biosensors based on these two aspects, with advantages and disadvantages of each aspect introduced herein. 相似文献
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特定序列脱氧核糖核酸电化学生物传感器进展 总被引:16,自引:0,他引:16
对当今电分析化学中的研究热点之一-脱氧核糖核酸(DNA)的电化学生物传感技术的最新进展进行了综述,评述了其发展前景。 相似文献