Digital particle holographic system for measurements of spray field characteristics

A digital particle holographic system for measurements of spray fields is presented. A double exposure hologram recording system with a synchronization system for time control is established, resulting in digital holograms that can be quickly recorded. To process recorded holograms, the correlation coefficient method is used for focal plane determination of particles. To remove noise and improve the quality of holograms and reconstructed images, a Wiener filter is adopted. The two-threshold and image segmentation methods are used for binary image transformation. For particle pairing, the match probability method is adopted. The proposed system is applied to a spray field, and three-dimensional velocities and sizes of spray droplets are measured. Measurement results from the digital holographic system are compared to those made by laser instruments, which prove the feasibility of the proposed in-line digital particle holographic system as a good measurement tool for spray droplets.     

数字全息显微术中重建物场波前的相位校正

数字全息显微术克服了传统光学显微术无法直接提取样品相位信息的缺点,可以对活体细胞组织等相位型生物样品进行定量测量和有效观察。但在数字全息显微成像过程中,像场弯曲会对再现像相位分布的测量和观察产生影响。提出一种采用相位相减来校正数字全息再现像像场弯曲的方法。通过在样品加入前后两次拍摄全息图,并对数值重建像分别进行去包裹运算再令其相减,即可实现对像场弯曲的有效校正,对蝉翼和大蒜表皮细胞等相位型物体进行测量,并采用数值校正和相位相减两种方法对像场弯曲进行校正。与现有的数值校正方法相比,利用相位相减获得样品三维相位信息的方法更为简单、可靠,是校正像数字全息再现场弯曲的有效方法。     

Securing complex and multi-plane data in a lens-less digital holographic information system that uses position-phase-shifting geometry

A digital holographic information system can process complex three-dimensional (3-D) object information. We demonstrate a scheme for securing complex and 3-D information in the context of in-line digital holography. Double random phase encoding in the free-space propagation domain of light is used to secure the complex information. Encrypted in-line digital holograms are recorded using the position-phase-shifting method. The encrypted complex image at the CCD recording plane is retrieved from the real-valued digital holograms, and is used for decryption. The robustness of the method has also been studied for various securing keys used in the method against blind decryption. A layer-by-layer information retrieval from the encrypted digital hologram is also discussed. The method can also be used to secure digital complex information in a virtual optics modality using holographic principles.     

Controlling image size as a function of distance and wavelength in Fresnel-transform reconstruction of digital holograms

A method for controlling the size of amplitude and phase images reconstructed from digital holograms by the Fresnel-transform method is proposed and demonstrated. The method can provide a constant reconstruction pixel width in the reconstructed image plane, independent of the recording and reconstruction distance. The proposed method makes it possible to maintain the size of an object for a sequence of digital holograms recorded at different distances and, therefore, to subtract phase maps for an object recorded at different distances. Furthermore, the method solves the problem of superimposition in multiwavelength digital holography for color display and holographic interferometry applications.     

Study on the simplified phase-shifting digital holographic microscopy

In this paper, a new simplified technique for effectively eliminating the zero order and the conjugate virtual image in digital holographic microcopy, which makes use of two-step phase-shifting method of just recording two holograms and an intensity image of object wave, is proposed. Meanwhile, combined with the principle of making full use of spatial bandwidth of the CCD sensor by in-line lens-less Fourier holographic recording geometry, the theory and experimental methods to increase the resolution of the reconstructed image in digital holography by using phase-shifting technique are detailedly analyzed. At end, the validity and availability of this technique has been demonstrated through the off-axis and in-line Fourier transform recording geometry. The study provides some theoretical and experimental guidance for the design and operation of a digital holographic microscopy system.     

数字显微全息中记录参数对颗粒测量影响的数值模拟

数字显微全息技术由于具有三维、非接触和实时测量微小空间内流场的能力, 已引起了国内外学者的广泛关注. 利用数字显微全息方法测量微通道流场时, 记录距离、颗粒尺寸、颗粒浓度、入射光波长、CCD分辨率等参数会对颗粒重建结果产生重要影响. 为了评估颗粒浓度和样本空间深度对重建结果的影响, 本文开展了数值模拟研究. 采用基于洛伦兹-米散射理论的程序产生不同浓度的颗粒全息图, 用小波变换重建算法对其进行重建. 结果表明: 在样本空间深度为24 μm 时, 颗粒浓度n_s在3.44×10⁵ mm^-3–13.77×10⁵ mm^-3 范围内时, 颗粒重建率E_p随着颗粒浓度n_s 的增大而迅速减小, 在13.77×10⁵ mm^-3–55.08×10⁵ mm^-3范围内时, 颗粒重建率E_p 随颗粒浓度n_s增大而缓慢减少. 在颗粒浓度n_s (13.77×10⁵ mm^-3) 保持不变时, 颗粒重建率E_p与样本空间深度满足单调递减的线性关系. 当阴影密度不变时, 重建率的变化呈现一定的规律性:当深度L较小时, 样本空间深度对颗粒重建的影响要比颗粒浓度的影响大; 当深度L较大时, 颗粒浓度对颗粒重建的影响较大. 关键词： 数字显微全息 颗粒浓度 粒径误差 位置误差     

A new multiple-exposure scheme for full-color full-parallax holographic stereogram

We propose a new multiple-exposure scheme for full-color full-parallax holographic three-dimensional (3D) printer, in which two of three primary-color holograms are exposed at the same location on the recording medium. In this paper, the optimal combinations of colors for multiple exposure are experimentally derived. In addition, it is shown that the proposed method enables higher brightness, better color reproducibility, and high resolution through the experiment of recording and measurement of color-chart holographic stereogram.     

粒子场数字全息诊断中的再现算法研究

 粒子场的数字全息诊断中,良好的再现算法能够在较短的时间内给出高质量的再现像。利用标准粒子板模拟单层面的粒子场,使用大面阵CCD实现同轴数字全息记录,得到了大尺寸的数字全息图。针对4种数字全息再现算法,本文从再现图像的质量、再现全息图的大小和计算速度3个主要方面进行了比较研究,结果表明角谱算法(FFT-AS)具有再现图像背景均匀,再现结果中无物理图像压缩,可以再现大尺寸的全息图且具有较快计算速度的优点,适合于粒子场同轴数字全息图的再现计算。     

Tracking particles in four dimensions with in-line holographic microscopy

We describe a simple holographic method that has enabled us to capture as a single data set the trajectories of micrometer-sized objects suspended in water. By subtracting consecutive holograms of a particle suspension and then adding these difference holograms, we constructed a final data set that contains the time evolution of the particle trajectories free from spurious background interference effects. The method is illustrated by a recording of the motion of 5-10-microm diameter algae in water.     

An experimental investigation of three-dimensional particle aggregation using digital holographic microscopy

The tendency of particles to aggregate depends on particle-particle and particle-fluid interactions. These interactions can be characterized but it requires accurate 3D measurements of particle distributions. We introduce the application of an off-axis digital holographic microscopy for measuring distributions of dense micrometer (2 μm) particles in a liquid solution. We demonstrate that digital holographic microscopy is capable of recording the instantaneous 3D position of particles in a flow volume. A new reconstruction method that aids identification of particle images was used in this work. About 62% of the expected number of particles within the interrogated flow volume was detected. Based on the 3D position of individual particles, the tendency of particle to aggregate is investigated. Results show that relatively few particles (around 5–10 of a cohort of 1500) were aggregates. This number did not change significantly with time.     

Internal inspection of semi-transparent objects by digital holographic micro-tomography

The combined use of digital holographic microscopy and computer tomography, here named digital holographic micro-tomography, is used to examine the interior of transparent channels. The proposed method is used to identify internal obstacles inside of transparent troughs having slightly different refractive index. The method is based in the acquisition of a set of digital holograms of the specimen whereas it is axially rotated from 0° to 180°. The phase differences retrieved from the obtained holograms are the inputs to a computerised axial tomography procedure. The technique has been numerically modelled in order to find the optimal tomographic conditions and also to realise the minimum difference of refractive index the method could detect. The obtained results show the feasibility of the proposed method for the non-destructive evaluation of transparent micro-objects.     

Holographic particle image velocimetry: analysis using a conjugate reconstruction geometry

Holographic recording techniques have recently been studied as a means to extend two-component, planar particle image velocimetry (PIV) techniques for three-component, whole-field velocity measurements. In a similar manner to two-component PIV, three-component, holographic PIV (HPIV) uses correlation-based techniques to extract particle displacement fields from double-exposure holograms. Since a holographic image contains information concerning both the phase and the amplitude of the scattered field it is possible to correlate either the intensity or the complex amplitude. In previous work we have shown that optical methods to compute the autocorrelation of the complex amplitude are inherently more tolerant to aberrations introduced in the reconstruction process, Coupland, Halliwell, Proc. Roy. Soc. 453 (1960) (1997) 1066. In this paper we introduce a new method of holographic recording and reconstruction that allows a constant image shift to be introduced to the particle image displacement. The technique, which we call conjugate reconstruction, resolves directional ambiguity and extends the dynamic range of HPIV. The theory of this method is examined in detail and a relationship between the image and object displacement is derived. Experimental verification of the theory is presented.     

Holography and micro-holography of particle fields: A numerical standard

Holography is an ‘old’ technique for studying the behavior of clouds of droplets which finds a new interest with CCD cameras and real-time numerical reconstruction. Furthermore, the continued progress in camera characteristics (sensitivity, pixel number, digitalization level, and so on) opens the way to more accurate recording of the interference field. To gain a deep understanding of the technique, as well as an evaluation of the performance and limitations of digital holographic particle measurements under various conditions, standard holograms are required. In this paper, a general numerical standard of holograms of fields of particles based on rigorous near-field Lorenz–Mie scattering theory is presented. This theory makes possible the computation of holograms of fields of particles with an arbitrary number of particles of arbitrary size, arbitrary refractive index, arbitrary recording distance (near-field or far-field), and an arbitrary collecting angle (forward, off-axis, or backward scattering light). Several calculation examples are also given for the code validation and possible applications, including a new possible way to simultaneously measure the size, location, and refractive index of particles.     

Polarization phase shifting in digital holographic microscopy

In the present work we have made use of polarization phase shifting in digital holographic microscopy (DHM) for three dimensional phase profiling of transmissive and reflecting microscopic samples. The Mach–Zehnder arrangement with proper polarizing elements (polarizer-masked cube beam splitter, quarter wave plate and a linear polarizer) is used for recording the phase-shifted digital holograms. The suggested procedure is simple and accurate and obviates the need of piezo devices for phase shifting. The phase profile of the specimen is reconstructed from the holograms by using standard phase shifting algorithms.     

Correction of quadratic phase error in two-wavelength digital holographic interferometry using laser diodes

We present the correction of a quadratic phase error in two-wavelength digital holographic interferometry using laser diodes. This phase error arises from numerical reconstructions of wavefronts from digital holograms based on the Fresnel diffraction integral. To correct the quadratic phase error, it is numerically produced by computer on the basis of the theoretical prediction and is subtracted from the phase difference map in two-wavelength digital holographic interferometry. Experimental results show that the method of correction in this paper is useful for two-wavelength digital holographic interferometry using laser diodes.     

State of the art of color interferometry at ONERA

At ONERA-Lille center a lot of studies have been conducted to characterize complex flows using an optical method based on differential interferometry with Wollaston prism and white polarized light source. Several applications are presented in two-dimensional and axisymmetric flows and in a gaseous mixture where the two gases interface is submitted to acceleration. Then, real-time color holographic interferometry (RCHI) has been developed to obtain the refractive index itself in two dimensional wake flow. The last improvements concern the extension of this method for analyzing three dimensional flows. The authors present a specific setup defined in a single sight direction, the aim being to reproduce the same optical setup along several sight directions, each shifted by a given angle. This optical technique uses reflection holograms where the diffraction efficiency of plates is strongly influenced by the variations in the gelatin thickness produced during the holograms treatment. Problems are discussed and solutions are proposed to control the gelatin shrinkage for two different types of used holograms. The results obtained in a one sight direction make it possible to build in the future an optical setup allowing several simultaneous line-of-sight optical measurements.     

Stereophotogrammetric 3D shape measurement by holographic methods using structured speckle illumination combined with interferometry

We present a unique combination of the numerical three-dimensional (3D) reconstruction of the shape of an object with interferometric deformation measurements. Two cameras record several holograms of an object that is illuminated by structured illumination. This illumination is realized by speckle patterns. To improve the image quality, an inplace speckle reduction technique is combined with the structured illumination to reduce the effect of disturbing subjective speckles which appear in the reconstructed images. Stereophotogrammetric methods are applied to extract the 3D surface information of the object out of the reconstructed images. Since the recording is done by holography and because stereophotogrammetry enables a pointwise correlation between the two views, it is possible to combine other holographic techniques with the reconstructed 3D shape. This is demonstrated by an interferometric deformation measurement of an object cooling down. The resulting interferometric fringes are mapped onto the reconstructed 3D surface. Hence, the proposed method enables automatic and dense matching of interferometric fringe-maps recorded by spatially separated holograms onto the surface of the object, which has not yet been realized by existing techniques.     

A review of aspects relating to the improvement of holographic memory technology

Results of studying a holographic memory to write/read digital data pages are presented. The research has been carried out in Novosibirsk, Russia. Great attention was paid to methods of improving recording density and the reliability of data reading, the development of ‘dry’ photopolymers that provide recording of superimposed three-dimensional phase holograms, and the designing of parallel optic input large-scale integration (LSI) for reading and logical processing of data arriving from the holographic memory.     

Optical noise reduction for dc-removed coaxial holographic data storage

A method of reconstructing positive and negative images from Fourier holograms recorded without the dc components is demonstrated by use of a coaxial holographic storage system. Reconstructed images are obtained by adding a phase-modulated dc component of the signal beam on reading. Contrast reversal of the reconstructed images can be achieved by reversing the readout reference pattern. This method can realize not only optical noise reduction but also less consumption of the dynamic range of the recording medium, potentially contributing to increasing the number of multiplexed holograms.