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LIUJia-ming YANGTian-long YANHai-gang SHENLi-shuang LILong-di 《高等学校化学研究》2003,19(4):395-398
A new highly sensitive solid substrate room temperature phosphorimetry for the determination of trace titanium is proposed based on the sensitization of Triton X-100 to the SS-RTP intensity of 4, 5-dibro-mophenylfluorone-titanium complex adsorbed on the filter paper substrate modified by gelatin. When Triton X-100 was added into the luminescence system, the RTP intensity was raised 3 times stronger than that of the system without Triton X-100. The linear dynamic range of the new method is 0. 64 ~ 3.2 fg/spot (0. 4 μL) with a detection limit of 12.8 ag/spot, and the regression equation of the working curve is △Ip = 482. 0 119.5mTi(Ⅳ) (fg/spot), the correlation coefficient r= 0. 9992, n = 6. The phosphorescence lifetime (r= 0. 85 ms) was also determined. The recoveriesCand RSD) for the determinations of titanium in human hair and tea samples were 101.0% (3. 0%) and 99. 97% (4. 2%), respectively. 相似文献
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本文提出间三联苯和对三联苯的二阶导数-固体基质室温燐光法(d2SS-RTP)。本法在λax=288nm,用448nm处正峰和460nm处负峰的峰峰高度值定量测定间三联苯,对三联苯不干扰测定.线性范围0.46~46ng.检出限为0.1ng/斑点.对三联苯用526nm处正峰和548nm处负峰的峰峰高度值测定.间三联苯不干扰.线性范围0.46~46ng.检出限0.07ng/斑点. 相似文献
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固体基质室温燐光分析法测定痕量银 总被引:2,自引:0,他引:2
基于AgCl·PVA·Ag+ 吸附FIn- 所形成的离子缔合物AgCl·PVA·Ag+ ·FIn- 能在滤纸基质上发射强而稳定的室温光信号的特性 ,建立了以滤纸为基质的固体基质室温光测定痕量银的新方法。该离子缔合物对应于聚乙烯醇 (PVA)存在下Fajans(法扬斯 )法的终点。在一定条件下 ,离子缔合物的光强度与吸附层的Ag+ 含量成正比 ,线性范围为 1.72~ 8.6× 10 - 1 2 g 斑 ,(0 .4 μL 斑 ) ,工作曲线对应的回归方程ΔIp =2 80 .81+35 4 5mAg+ (10 - 1 2 g 斑 ) ,n =6 ,相关系数r =0 .9995。该方法快速、灵敏、准确。用于人发、茶叶中银的测定 ,与AAS法基本相符。 相似文献
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Changqing Lin Lihong Zhang Zhiyong Zheng Xuan Lin Liping Lin Xinxing Wang Shaoqin Lin Jiaming Liu 《中国化学》2010,28(11):2233-2239
Under the conditions of reacting at 100°C for 8 min and irradiating with the infrared light for 15 min, methylene blue (MEB) could emit weak room temperature phosphorescence (RTP) on the cellulose acetate membrane (CAM) using Pb2+ as the ion perturber in the NH3‐NH4Cl (pH=9.80) solution. MEB was oxidized by H2O2, which caused the RTP of the system to enhance. Cu2+ could catalyze the reaction of H2O2 oxidizing MEB, which caused the RTP of the system to enhance sharply. The ΔIp (Ip0?Ip, the Ip0 and Ip were RTP intensity of the blank reagent and RTP intensity of the test reagent, respectively) of the catalytic system was 133.6, which was 4.1 times larger than that without infrared light irradiation (33.1). Its ΔIp was proportional to the content of Cu2+. Thus, a new catalytic solid substrate room temperature phosphorimetry (SSRTP) for the determination of trace copper has been established. The limit of quantization (LOQ) of this method was 0.12 ag·spot?1 (sample volume: 0.40 µL·spot?1, corresponding concentration: 3.0×10?16 g·mL?1), showing high sensitivity. This method not only has been successfully applied to the determination of trace copper in the tobacco, tea and human serum, but also could predict human diseases. The catalytic reaction was first order reaction, whose activation energy (E) was 18.17 kJ·mol?1 and rate constant (k) was 3.4×10?4 s?1. At the same time, the mechanism of catalytic SSRTP for the determination of trace copper was discussed. 相似文献
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Jia Ming LIU? Yun Xiong WU Shao Xian LIN Fei Ming LI Xiu Mei SHI Zhong Bin SHI Guo Hui ZHU Zhi Ming LI Xiao Mei HUANG Department of Chemistry Environmental Science Zhangzhou Normal College Zhangzhou Department of Food Chemical Engineering Zhangzhou Institute of Technology Zhangzhou 《中国化学快报》2006,17(12):1582-1584
There are some methods for the determination of trace Hg2 such as atomic fluorescence spectrometry (LD: 2.0×10-11 g mL-1)1, catalytic discoloration photometric method (LD: 1.2×10-12 g mL-1)2, bulk optode modified by dithizone (LD: 8.0×10-9 g mL-1)3, c… 相似文献
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基于痕量Se4+催化K2S2O8氧化靛红(Isa)的灵敏反应而导致体系的磷光剧烈猝灭的学术思想,建立了一种高灵敏(检出限(LD)为0.015 pg/mL)催化固体基质室温磷光法(SS RTP)测定痕量Se(IV)的新方法。方法的线性范围为4.00×10–8~4.00×10–6 μg/mL。工作曲线的回归方程为△IP=2.948+63.24CSe(IV)(pg/mL),n = 7。相关系数( r )为 0.9997。方法已成功用于水样中痕量Se(IV)的测定,结果与紫外分光光度法相吻合,并探讨了测定痕量Se(IV)的反应机理。 相似文献
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A Triton X-100-4.0G-D (4.0G-D refers to a 4.0-generation dendrimer) was brought forward as a new phosphorescence labeling reagent. Two types of specific affinity adsorption (AA) reactions (direct method and sandwich method) were carried out between the labeling product of Triton X-100-4:0G-D-Wheat germ agglutinin (WGA) and alkaline phosphatase (ALP), the product of AA reaction preserved the good characteristics of room temperature phosphorescence (RTP) of 4.0G-D and △Ip of the product was proportional to the content of ALP. According to the fact stated above, a new method for the determination of trace ALP by affinity adsorption solid substrate-room temperature phosphorimetry (AA-SS-RTP) was established on the basis of WGA labeled with the Triton X-100-4.0G-D. The detection limits were 0.20 ag·spot^-1 (corresponding concentration: 5.0×10^-16 g·mL^-1, namely 5.0×10^-18 mol·L^-1) for a direct method and 0.14 ag·spot^-1 (corresponding concentration: 3.5×10^-16 g·mL^-1, namely 3.5×10^-18 mol·L^-1) for a sandwich method, respectively. For their high sensitivity, good repeatability and high accuracy, the direct method and sandwich method have been successfully appfied to determine the content of ALP in human serum, and the results were coincided with the clinical detection results of the enzyme-linked immunosorbent assay method by the Zhangzhou Hospital of Traditional Chinese Medicine. Meanwhile, the mechanism for the determination of trace ALP by AA-SS-RTP was discussed. 相似文献
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A novel peroxyoxalate chemiluminescence system has been designed for the determination of Triton X‐100 (TX‐100), in which a hydrophobic fluorescent conjugated polymer, poly[2,5‐bisnonyloxy‐1,4‐phenylene‐ethynylene‐9,10‐anthrylene] (PPEA) was employed as a fluorophor. A strong enhanced intensity of chemiluminescence (CL) was observed in the presence of TX‐100, due to the improved emission efficiency of PPEA in the presence of TX‐100. Under optimum conditions, the detection range of Triton X‐100 is between 1.0×10?7 and 1.0× 10?4 mol·L?1, with a detection limit at 6.0×10?8 mol·L?1. The relative standard deviation is 2.4% (n=6) for 1.0×10?6 mol·L?1 Triton X‐100. This method provides satisfying results in the detection of TX‐100 in nature water and biological samples with high sensitivity and wide linear range. 相似文献
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同步扫描-微乳状液增稳室温磷光法同时测定痕量多环芳烃的研究 总被引:1,自引:0,他引:1
同步扫描-微乳状液增稳室温磷光法同时测定痕量多环芳烃的研究杨欣,董川,魏雁声,晋卫军,刘长松(山西大学化学系,太原,030006)关键词同步扫描-微乳状液增稳室温磷光法;化学除氧技术;多环芳烃1978年Vo-Dinh等[1]将固定波长同步扫描技术用于... 相似文献
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亮黄-曲通X-100体系共振瑞利散射法测定蛋白质 总被引:15,自引:0,他引:15
基于在TrtionX-100存在下,蛋白质对有机染料亮黄瑞利光散射的增强作用。拟定了测定蛋白质的瑞利光散射光,由于添加了TritonX-100,对不同的蛋白质,测定的灵敏度提高了5-11.8倍,线性范围在0-5.0mg/L之间,最低检测限为10.2μg/L。 相似文献
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基于硼砂缓冲液中聚丙烯酰胺(PAM)存在下,邻苯三酚红(PGR)于滤纸固体基质上能发射强而稳定的室温燐光,PAM可活化钴离子催化H2O2氧化PGR反应,使PGR的室温燐光猝灭,从而建立了测定痕量钴的新方法。该方法的线性范围为4.8~960(×10-18g/斑)Co2+(浓度范围0.012~2.40×10-9g/L,0.4μL/斑),工作曲线的回归方程ΔIp=12.76+0.1248 mCo2+(×10-18g/斑),n=7,相关系数r=0.9993;检出限为1.2×10-18g/斑(对应浓度为3.0×10-12g/L)。对4.8和960(×10-18g/斑)Co2+重复测定11次(0.4μL点样量),RSD分别为3.6%和4.7%。用PAM比不加PAM时ΔIp增加4.6倍。本方法灵敏、准确、简便快速、重现性好,用于党参、当归、川芎、金银花和VB12中的痕量钴测定,结果满意。 相似文献
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LIU Jia-Ming LIU Zhen-Bo LI Zhi-Ming HE Hang-Xia LIN Wei-Nü HUANG Ya-Hong WANG Fang-Mei 《中国化学》2008,26(9):1635-1640
In the presence of heavy atom perturber Pb2+, silicon dioxide nanoparticle containing fluorescein isothiocyanate (FITC-SiO2) could emit a strong and stable room temperature phosphorescence (RTP) signal on the surface of acetyl cellulose membrane (ACM). It was found in the research that a quantitative specific affinity adsorption (AA) reaction between triticum vulgare lectin (WGA) labeled with luminescent nanoparticle and glucose (G) could be carried on the surface of ACM. The product (WGA-G-WGA-FITC-SiO2) of the reaction could emit a stronger RTP signal, and the ΔIp had linear correlation to the content of G. According to the facts above, a new method to determine G by affinity adsorption solid substrate room temperature phosphorimetry (AA-SS-RTP) was established, based on WGA labeled with FITC-SiO2. The detection limit (LD) of this method calculated by 3Sb/k was 0.47 pg•spot-1 (corresponding to a concentration value 1.2×10-9 g•mL-1, namely 5.3×10-9 mol•L-1), the sensitivity was high. Meanwhile, the mechanism for the determination of G by AA-SS-RTP was discussed. 相似文献
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