一种不对称菁染料及其与不同结构DNA的相互作用

为考察小分子配基与不同核酸结构的结合机理,发展新的核酸探针分子,合成了一种新型一次甲基不对称菁染料(MTP).吸收光谱、荧光光谱及圆二色光谱研究结果表明,MTP可与平行和混合平行G-四链体DNA(如c-myc和22AGK+)较强地结合,并引起130~180倍的荧光增强;其与单/双链DNA作用较弱,导致40~60倍荧光增强;而与反平行G-四链体DNA(如TBA和22AGNa+)的作用最弱,只引起15~25倍荧光增强;以上结果表明MTP可作为荧光探针分子用于区别不同结构的核酸分子.结合机理研究表明,平行和混合平行G-四链体DNA优先通过沟槽结合模式结合1分子MTP,再通过末端堆积模式结合另1分子MTP.     

荧光标记染料

荧光标记染料在生命科学领域具有极大的应用前景,基因芯片的研究开发使荧光染料应用于药理研究、药物毒性、药物靶标研究、医学诊断等领域。本文对荧光素、若丹明、菁染料等核酸及蛋白质分析检测用荧光标记染料的结构、性能及其应用特点作了较为详细的介绍。     

Triphenylmethane dyes as fluorescent probes for G-quadruplex recognition

Triphenylmethane (TPM) dyes normally render rather weak fluorescence due to easy vibrational deexcitation. However, when they stack onto the two external G-quartets of a G-quadruplex (especially intramolecular G-quadruplex), such vibrations will be restricted, resulting in greatly enhanced fluorescence intensities. Thus, TPM dyes may be developed as sensitive G-quadruplex fluorescent probes. Here, fluorescence spectra and energy transfer spectra of five TPM dyes in the presence of G-quadruplexes, single- or double-stranded DNAs were compared. The results show that the fluorescence spectra of four TPM dyes can be used to discriminate intramolecular G-quadruplexes from intermolecular G-quadruplexes, single- and double-stranded DNAs. The energy transfer fluorescence spectra and energy transfer fluorescence titration can be used to distinguish G-quadruplexes (including intramolecular and intermolecular G-quadruplexes) from single- and double-stranded DNAs. Positive charges and substituent size in TPM dyes may be two important factors in influencing the binding stability of the dyes and G-quadruplexes.     

Templated self-assembly of wedge-shaped DNA arrays

We demonstrate the use of a one-dimensional template to control the shape of a two-dimensional array self-assembled from a minimal set of DNA tiles. A periodic single-stranded template seeds tile assembly. A unique vertex tile at the 5′ end of the template controls the positioning of edge and body tiles to create a wedge-shaped array. The vertex angle of the array is approximately 12°; edge lengths are of the order of 1 μm.     

Synthesis and post-synthetic derivatization of a cyanine-based amino acid. Application to the preparation of a novel water-soluble NIR dye

An efficient synthesis of a new fluorescent amino acid derived from a pentamethylene cyanine dye is described. Selective derivatization of its amino group with an original trisulfonated linker has led to a novel water-soluble NIR dye suitable for covalent labeling of biomolecules     

含碲唑杂环的不对称碳菁及方酸菁的合成与性质

以苯并碲唑季铵碘盐为原料，将其与３－乙基－２－（β－乙酰苯胺）乙烯工噻唑磺化季铵盐在无水吡啶中回流，得到４个含碲不对称碳菁染料，上述季铵盐与方酸的反应不 般杂环碱，只能发生１：１缩合反应，缩合产物与苯并噻唑碘盐在正丁醇／吡啶中反应即得含碲不对称方酸菁染料，该法避免了通常制备不对称方酸菁所带来的分离纯化的困难，从而提供了制备不对称方酸碲菁染料的通用方法，研究了不对称碲碳菁的“Ｂｒｏｏｋｅｒ偏差”，结     

Gemini型两亲分子超分子组装研究进展

综述了国内外对Gemini型两亲分子在超分子组装方面的研究进展。总结了Gemini分子的体相组装、界面组装以及近几年应用领域的研究进展;着重介绍了基因传递、纳米材料模板、降解剂、药物缓释以及胶凝剂的制备等方面的应用进展;对相关研究领域的主要研究成果做了一些探讨和分析;为更加深入探索和研究Gemini型两亲分子的超分子组装提供参考信息。     

超分子聚合物凝胶

作为非常重要的软物质材料,超分子聚合物凝胶代表了一个全新的概念和更复杂的凝胶体系.这种新型的超分子体系的构建,是基于多种非共价相互作用协同的多层次组装.即小分子构筑基元首先组装成为超分子聚合物,而这些非共价聚合物的多层次组装形成凝胶的纳米结构.超分子聚合物凝胶无论是在结构上,还是在性能上都具有很多崭新的特点.因此,尽管有关超分子聚合物凝胶的研究开展的时间还很短,这一体系所表现出的独特性以及巨大潜力已经引起科学家们越来越广泛的关注.本文简要综述了这一领域的最新进展.主要论述基于多种非共价相互作用的超分子聚合物凝胶的构建以及对其力学性能的调控.     

pH-Responsive Cy5 dyes having nucleophilic substituents for molecular imaging

pH-Responsive fluorescent pentamethine cyanine (Cy5) derivatives having nucleophilic substituents were synthesized. Cy5 derivatives 1-O having mercaptopropyl, hydroxypropyl, and aminopropyl groups on an indole nitrogen atom showed pH-dependent equilibria between a fluorescent open-ring structure (1-O) and a non-fluorescent closed-ring structure (1-C) in pH ranges 3–7, 6–7.5, and 8–9, respectively. pH-Responsive dyes 1a-C having a 1,3-thiazinane structure were easily internalized into A549 cells and converted to open-ring structure 1a-O in response to the relatively low pH of acidic compartments in the cells.     

溶液中菁染料的状态对其在AgBr表面吸附效应的量热研究

用半导体精密量热计测得25±0.01℃时溴化银从DMF-H₂O溶液中吸附噻碳菁染料(Ⅰ)的吸附热。发现由于溶液中染料聚集状态的不同导致吸附热明显不同,但吸附于溴化银表面的染料的聚集态则基本相同。     

含有萘酰亚胺的菁染料太阳能电池敏化剂的合成

通过Click反应把萘酰亚胺化合物连接到含有醛基的吲哚上,再通过醛基和吲哚碘盐的Knoevenagel缩合反应合成出带有萘酰亚胺部分的菁染料,用NMR、MS、元素分析、UV-Vis等方法对其结构和性能进行了表征和测试。     

Step-growth polymerization of traptavidin-DNA conjugates for plasmonic nanostructures

Here,we use two important biomaterials,protein and DNA,to construct self-assembled linear nanostructures through Watson-Crick base-paring of DNAs.We apply a simple magnetic separation method to purify traptavidin-DNA co njugates,and demonstrate synthesis of linear arrays of traptavidinDNA conjugates via the step-growth polymerization approach with pre-determined DNA sequences.Using the traptavidin-DNA array as a template,we assemble gold nanoparticles to form linear plasmonic nanostructures in a programmable manner.The traptavidin-DNA conjugates thus provide a convenient platform for one-dimensional assembly of biotinylated nanomaterials for many biomedical applications from drug delivery to bio-sensing.     

超分子模板法合成层状中孔结构氧化铝

利用烷基硫酸钠作为模板剂在室温下合成了层状中孔结构氧化铝,考察了表面活性剂碳链长度和表面活性剂混合比例对层状氧化铝中孔相结构参数的影响.当使用单一表面活性剂作为模板剂时,层状中孔结构氧化铝的层间距d及其层状结构的长程有序度随着表面活性剂碳链长度的增加而增加;若使用C₈H₁₇SO₄Na与C₁₄H₂₉SO₄Na混合表面活性剂作为混合模板剂,当C₁₄H₂₉SO₄Na摩尔分数x处在0～0.5时,层间距基本恒定,接近于具有二者平均碳链长度(n=11)的单一表面活性剂作为模板剂时的情形;当x>0.5时,层间距随x的增大而逐渐增大.通过提出的混合表面活性剂在无机层中的分子排列结构模型对该结果做了解释.     

合成的近红外花菁染料测定痕量血清蛋白质

染料结合法测定蛋白质在生化及临床分析中已有广泛应用,常用的染料有溴甲酚绿、溴酚蓝、考马斯亮蓝及铬天青S等,这些染料的最大吸收波长均处于可见光区.近年来,近红外染料越来越引起人们的注意.