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1.
Visible (Vis) and near-infrared reflectance (NIR) spectroscopy combined with chemometrics was explored as a tool to trace muscles from autochthonous and crossbreed pigs from Uruguay. Muscles were sourced from two breeds, namely, the Pampa-Rocha (PR) and the Pampa-Rocha x Duroc (PRxD) crossbreed. Minced muscles were scanned in the Vis and NIR regions (400–2,500 nm) in a monochromator instrument in reflectance. Principal component analysis (PCA), discriminant partial least square regression (DPLS), linear discriminant analysis (LDA) based on PCA scores and soft independent modelling of class analogy (SIMCA) were used to identify the origin of the muscles based on Vis and NIR data. Full cross validation was used as validation method when classification models were developed. DPLS correctly classified 87% of PR and 78% of PRxD muscle samples. LDA calibration models correctly classified 87 and 67% of muscles as PR and PRxD, respectively. SIMCA correctly classified 100% of PR muscles. The results demonstrated the usefulness of Vis and NIR spectra combined with chemometrics as rapid method for authentication and identification of muscles according to the breed of pig.  相似文献   

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3.
A rapid headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) method has been developed for the determination of anethole in serum samples. Anethole is a characteristic marker for the consumption of aniseed spirits. This method enabled the detection of anethole with a limit of detection (LoD) of 3.6 ng/ml and a limit of quantification (LoQ) of 5.3 ng/ml in serum samples with a good degree of precision intraday (2.8%) and interday (4.5%). Experiments were conducted with one volunteer, in which the subject consumed the alcoholic drink ouzo on 3 different days under controlled conditions. At defined intervals, blood samples were taken from the subject. Using these blood samples, the concentration-time profiles for anethole were determined. In blood samples taken from 50 drivers who claimed to have consumed drinks containing anethole (ouzo, raki and the German aniseed liqueur "Küstennebel") before the taking of the blood sample, anethole was detected in the serum in concentrations of between 5.4 and 17.6 ng/ml in 10 cases. This is the first report describing the qualitative and quantitative determination of a beverage-characteristic aroma compound - in this case anethole - in serum samples after consumption of alcoholic beverages.  相似文献   

4.
Recently, much attention has been given to congener analysis, which can be used to check the possibility of postoffence drinking claims in forensic toxicology. In this type of analysis, the information given by the defendant regarding the type, quantity, and time of consumption of a specific alcoholic beverage is used to calculate theoretically expected congener concentration in the blood and this is compared with the analytically determined concentrations in the blood sample. Many alcoholic drinks aromatized with essential oils of plants and fruits contain a specific congener, for example, anethole in aniseed drinks. The present study describes the GC procedure of anethole analysis in human plasma using SPE as the sample preparation method. The procedure involves the protein precipitation process, which generally degrades the protein–analyte complex, and SPE isolation of anethole from the examined materials. This analytical approach is proposed as a method of choice for the estimation of anethole concentration in human fluids after the consumption of alcoholic beverages and other foods containing the substance. The described method is characterized by a low LOD (8.33 ng/g) and a very high recovery (average recovery 98.37%) of the analyte.  相似文献   

5.
The chemical components of both Syrian aniseed and fennel seeds collected from Idlib and Aleppo regions were identified using GC/MS spectrometry. Thirty-four component fractions representing an average of 98.5% of anise seed extract were characterized. The major average components were (E)-Anethole, Estragole, Limonene, Fenchone, Linalool, para-Anisaldehyde, (Z)-Anethole, and methyl chavicol. Also, thirty-one component fractions representing an average of 99.46% of fennel seed extract were identified. The major components were α-Pinene, α-Phellandrene, Fenchone, Methyl chavicol, Estragole, (Z)-Anethole, Limonene, 1,8-Cineole, Anisole-p-allyl, E,E-Farnesene, Estragole, (E)-Anethole, Anisaldehyde, and Carvacrol. The extract of aniseed was characterized by higher amounts of trans-anethole (52.2%) than fennel extract (38.3%). The integral antioxidant capacity (IAC) of two different extracts from both aniseed and fennel seeds have been determined using photochemiluminescence assay (PCL). The sum of antioxidant capacity (IAC) of hydrophilic and hydrophobic components in both aniseed and fennel seeds was determined, as a total equivalent per gram of dry material (nmolTE/gDM) at 166.69 ± 0.42 and 363.28 ± 1.11 nmol for hydrophilic and hydrophobic components, respectively. Eight major phenolic compounds known as possible antioxidant sources with their concentrations have been identified in both aniseed and fennel seeds by HPLC and GC–MS analysis.  相似文献   

6.
LC Determination of Anethole in Aniseed Drinks   总被引:1,自引:0,他引:1  
A method for the determination of anethole in aniseed drinks by high performance liquid chromatography (HPLC) is proposed. A C-18 column and a methanol-water (80:20, v/v) mobile phase with isocratic elution were used. UV detection at 257 nm was carried out. Validation was performed according to the EURACHEM guidelines, considering precision, trueness, robustness, linearity and sensitivity. The detection and quantification limits were 2.3 and 7.7 μg L−1, respectively. Recoveries of 95–101% were obtained. Repeatability, calculated as relative standard deviation was 0.9%. Reproducibility under intermediate precision conditions, expressed as relative standard deviation, was 1.6%. The method was compared with the gas chromatographic one, proposed by the European regulations.  相似文献   

7.
采用ab initio HF, MP2方法和密度泛函理论方法, 对具有D2h和D4d构型的膦配体稳定的过渡金属团簇[Au@Au8(PR3)8]3+(R=Me, OMe, H, F, Cl, CN)进行了几何结构、 电子结构及团簇稳定性等方面的研究. 计算表明, 与D2h构型相比, D4d构型更稳定, 两者能量相差约5~10 kJ/mol. SVWN局域泛函能够对团簇的几何结构给予较准确的描述, MP2方法对团簇的结构参数有所低估, 而离域和杂化泛函则过高地估计了团簇的结构参数. 电子结构分析表明, 中心Au原子与外围的Au原子之间通过 d 电子的成键作用构成团簇内核[Au@Au8]3+, [Au@Au8]3+与PR3配体则通过"σ给予/π反馈"模式成键. PR3配体与[Au@Au8]3+的结合能够加强内核-外围Au原子间的成键作用, 缩小外围Au原子在成键上的差异, 增大前线轨道能级间隙, 从而提高团簇的稳定性. PR3配体中R基团供、 吸电子能力的变化对[Au@Au8(PR3)8]3+结构影响较小, 但对[Au@Au8]3+-PR3结合能影响较大. 能量分析显示, 不同PR3配体与[Au@Au8]3+之间具有相近的轨道作用能, 与R基团供、 吸电子能力相关的非轨道作用能成为影响两者连接牢固程度的决定因素.  相似文献   

8.
Oral health problems may occur as a result of the ingestion of acid drinks. The objective of this in vitro study was to quantify and screen the concentration of potassium (K), phosphorus (P), calcium (Ca), magnesium (Mg), manganese (Mn), zinc (Zn), iron (Fe), copper (Cu), barium (Ba), lead (Pb), arsenic (As), cadmium (Cd), aluminum (Al), cobalt (Co), chromium (Cr), molybdenum (Mo), sodium (Na), nickel (Ni), selenium (Se), and vanadium (V) released from bovine incisors during an erosive challenge at different times of exposure when immersed in Coca-Cola™, orange juice, and grape juice. A total of 240 samples of bovine incisor teeth were used for the erosive challenge and allocated in groups. Digestion of drinks was performed using microwave-assisted digestion. The content in acidic drinks was monitored before and after the erosive challenge at exposure times of 1, 5, and 60 min using inductively coupled plasma optical emission spectrometry (ICP OES). The drinks’ pH varied slightly during the erosive challenge but remained below the critical value of pH 5 to cause tooth demineralization. The concentrations of elements released from the bovine incisors during the in vitro erosive challenge depend on exposure times when immersed in acidic beverages. For some elements such as Ca, Mn, Zn, Fe, Cu, Ba, Pb, As, and Cd, quantified in acidic drinks, grape juice had greater erosive potential than Coca-Cola™ and orange juice. Quantification and monitoring of chemical elements in bovine teeth can be performed considering a longer erosive time and other types of acidic drinks. Further analysis using human teeth is still not available and must be conducted. The demineralization of teeth not only occurs in acidic beverages; physical and chemical factors play other roles and should be investigated.  相似文献   

9.
采用从头计算MP2方法和密度泛函理论方法,对过渡金属团簇[PdAu8(PR3)8]2+(R=Me,OMe,H,F,Cl,CN)的几何结构、电子结构以及团簇各组成部分之间的结合能进行了研究.MP2方法和SVWN局域泛函能够对团簇的结构给予准确的描述,而离域泛函BP86,PBE,BLYP和杂化泛函B3LYP则过高地估计了团簇的几何结构参数.电子结构研究表明Pd,Au原子通过d电子的成键作用构成团簇内核[PdAu8]2+,[PdAu8]2+与PR3配体则通过"σ给予/π反馈"模式成键.PR3配体与[PdAu8]2+的结合能够加强Pd-Au之间的成键作用,增大前线轨道能级间隙,从而提高团簇的稳定性.PR3配体中 R 基团供、吸电能力的变化对[PdAu8(PR3)8]2+结构的影响较小,但对[PdAu8]2+ -pR3结合能的影响较大.能量分析显示不同PR3与[PdAu8]2+之间具有相近的轨道作用能,与R基团供、吸电能力相关的非轨道作用能成为影响两者连接牢同程度的决定因素.  相似文献   

10.
Three different polyhedral oligomeric silsesquioxanes (POSS), trisilanolphenyl polyhedral oligomeric silsesquioxane (T‐POSS), octaaminophenyl polyhedral oligomeric silsesquioxanes (OAPS), and octaphenyl polyhedral oligomeric silsesquioxanes (OPS) were incorporated into phenolic resin (PR), respectively; PR/POSS composites were successfully prepared, and the properties of PR/POSS composites were studied. The limiting oxygen index (LOI), cone calorimeter, and thermal gravimetric analysis (TGA) were used for the estimation of flame retardancy and thermal stability. Oxyacetylene flame test and flexural strength test were used to study the ablative and mechanical properties of the PR/POSS composites. The results indicated that T‐POSS was more effective in improving the flame retardancy of PR than OAPS or OPS. Meanwhile, compared with pure PR, the second line ablation rates of PR/4% T‐POSS, PR/4% OAPS, and PR/4% OPS were significantly reduced by 53.3%, 61.9%, and 40.0%, respectively. In addition, the thermal stability and flexural strength of PR/4% T‐POSS were significantly higher than that of all other PR composites.  相似文献   

11.
Physochlainae Radix (PR) is an essential herbal medicine that has been generally applied for treating cough and asthma. In this study, a comprehensive strategy for quality evaluation of PR from different origins was established by integrating qualitative identification, quantitative analysis, and chemometric methods. A total of 58 chemical components were identified by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS), and a sensitive and rapid UHPLC-QqQ-MS/MS method was established for the simultaneous determination of 12 compounds. In addition, multivariate statistical analysis was applied for discriminant analysis to compare the differences among 30 batches of PR samples. The results showed that the 30 batches of PR collected from four provinces could be clustered into three categories, in which scoparone, protocatechuic acid, tropic acid, and scopolin were important components to distinguish the primary and non-primary producing areas, as well as superior and inferior products of PR. Chemometric results were consistent and validated each other, and systematically explained the intrinsic quality characteristics of PR. This study first demonstrated that LC-MS combined with multivariate statistical analysis, provided a comprehensive and effective means for quality evaluation of PR.  相似文献   

12.
《Analytica chimica acta》2002,455(2):253-265
Human scalp hair samples of drug-free subjects and drug abusers (heroin and cocaine-heroin abusers) were analysed for trace metals by flame atomic absorption spectrometry (FAAS), flame atomic emission spectrometry (FAES) and electrothermal atomic absorption spectrometry (ETAAS). The classification of drug-free subjects and drug abuses groups with four multivariate methods using the metal contents in hair samples as discriminant variables has been discussed. Principal component analysis (PCA), cluster analysis (CA), linear discriminant analysis (LDA) and soft independent modelling of class analogy (SIMCA) allow distinguishing the two groups correctly. However, predictions by SIMCA are less satisfactory. Thirteen elements (Ag, Al, Ca, Cd, Cr, Cu, K, Mg, Mn, Na, Ni, Pb, and Zn) were determined by FAAS/FAES/ETAAS in 53 hair samples (16 samples of drug-free people and 37 samples of drug abusers). Human hair samples were prepared as aqueous slurries as sample pre-treatment and they were analysed using the slurry sampling technique. The half-range central value transformation was novelty used as data pre-treatment to homogenise the data. Grouping in the samples (drug-free people and drug abusers) were observed by using PCA and CA (squared Euclidean distance between objects and Ward method as clustering procedure). The application of LDA gave a correct recognition assignation percentage of 91.7 and 100.0% for the drug-free people and drug abusers, respectively, at a significance of 5%, while SIMCA offered recognition percentages of 83.3 and 91.3% for drug-free people and drug abusers, respectively, also at 5%. Finally, some studies were developed to classify heroin abusers and polidrug abusers (cocaine-heroin abusers) by the cited multivariate statistical methods. Recognition percentages of 90.9 and 100.0% were reached for heroin abusers and polidrug abusers groups, respectively, after LDA, while these percentages decreased to percentages lower than 90.0% when SIMCA was applied.  相似文献   

13.
Suliman FE 《Talanta》2002,56(1):175-183
An off-line optimization technique based on soft modeling methods has been used to determine the optimum conditions for the separation of eight carbonyl-2,4-dinitrophenylhydrazones by gas chromatography equipped with a nitrogen-phosphorous detector. In this method theoretical simulations of peak retention time and peak width at base-line are coupled to a factorial design to map the chromatographic response surface. The calculated retention times from isothermal data have been used to calculate the chromatographic parameters. A full 3(3) factorial design was used to locate the optimum conditions for the temperature program, utilizing initial oven temperature, T(i), the programming rate for the first ramp, PR(1), and the programming rate for the second ramp, PR(2) as independent variables. The optimum conditions obtained for these parameters were: T(i), 140 degrees C; PR(1), 12 degrees Cmin(-1); PR(2), 8 degrees Cmin(-1). The maximum analysis times achieved by this optimum was 10.6 min.  相似文献   

14.
Brominated flame retardants (BFRs) are the chemicals of high importance within the REAch framework. In addition to polybrominated diphenyl ethers (PBDEs), hexabromocyclododecane (HBCD) and tetrabromobisphenol A (TBBPA), other BFRs such as bromophenols, intermediates in FR formulation like bromoanilines, and their brominated and non-brominated by-products such as bromoanisoles, bromotoluenes, bromoalkanes and 1,5,9-cyclododecatriene, respectively should be monitored and controlled because of their toxicity and their very low odour and taste thresholds, below sub-nanogram-per liter levels. In the present study several analytical methods for the simultaneous determination, i.e., combining one single sample treatment and one analysis step, of these compounds in water have been developed, optimized and evaluated. The methods involve a (pre-concentration)-extraction technique, such as liquid-liquid (LLE), solid-phase (SPE), headspace (HS) extraction or solid-phase microextraction (SPME), followed by gas chromatography (GC)-mass spectrometry (MS) analysis with either electron capture negative ionization (ECNI) or electron impact (EI) as ionization techniques. ECNI is more sensitive than EI for analytes with more than one bromine atom. HS and SPME were previously optimized by means of a multifactorial experimental design. Extraction temperature and the liquid/headspace volume ratio were the most significant factors in HS extraction. In SPME, the variables studied were the nature of the fiber, the mode of extraction and the extraction temperature. Polydimethylsiloxane (PDMS) fibers appeared to be more suitable than carboxen-polydimethylsiloxane (CAR-PDMS) for the analysis of the target compounds with more than one bromine atom. The extraction of 2,4-dibromoaniline was only achieved in a direct immersion mode, in which the optimal extraction temperature was 60 degrees C. The methods LLE-GC-(ECNI)MS, LLE-GC-(EI)MS, SPE-GC-(ECNI)MS, SPE-GC-(EI)MS, HS-GC-(EI)MS and SPME-GC-(EI)MS were evaluated in terms of linearity, precision, detection limits and trueness. All methods, with the exception of HS-GC-(EI)MS, were linear in a range of at least two orders of magnitude, giving recoveries above 75% and detection limits at the low ng/L level for most of the target analytes. SPE-GC-(ECNI)MS is the most sensitive and reliable method for the determination of most of the bromine compounds, whereas SPE-GC-(EI)MS is the most suitable to quantify the three isomers of 1,5,9-cyclododecatriene. Both methods together with SPME-GC-(EI)MS (for qualitative confirmation) were applied to water samples from the Western Scheldt (The Netherlands), where 2,6-dibromophenol and 2,4,6-tribromoanisole could be detected at levels higher than their respective odour thresholds.  相似文献   

15.
The quality of foods has led researchers to use various analytical methods to determine the amounts of principal food constituents; some of them are the NMR techniques with a multivariate statistical analysis (NMR-MSA). The present work introduces a set of NMR-MSA novelties. First, the use of a double pulsed-field-gradient echo (DPFGE) experiment with a refocusing band-selective uniform response pure-phase selective pulse for the selective excitation of a 5–10-ppm range of wine samples reveals novel broad 1H resonances. Second, an NMR-MSA foodomics approach to discriminate between wine samples produced from the same Cabernet Sauvignon variety fermented with different yeast strains proposed for large-scale alcohol reductions. Third a comparative study between a nonsupervised Principal Component Analysis (PCA), supervised standard partial (PLS-DA), and sparse (sPLS-DA) least squares discriminant analysis, as well as orthogonal projections to a latent structures discriminant analysis (OPLS-DA), for obtaining holistic fingerprints. The MSA discriminated between different Cabernet Sauvignon fermentation schemes and juice varieties (apple, apricot, and orange) or juice authentications (puree, nectar, concentrated, and commercial juice fruit drinks). The new pulse sequence DPFGE demonstrated an enhanced sensitivity in the aromatic zone of wine samples, allowing a better application of different unsupervised and supervised multivariate statistical analysis approaches.  相似文献   

16.
Analysis of geological materials requires the use of matrix specific reference materials (RMs). Phosphate rocks (PR), the basic ingredient of phosphate fertilizers, are very important in agriculture. A project was undertaken to study the PRs of the Hazara Deposits of Pakistan. Due to the unavailability of phosphate rock reference materials (PR-RMs) with a large number of certified elements, validation of the results was problematic when carrying out elemental analysis of PR samples and their derived products. Therefore a parallel study was performed to determine the composition of three phosphate based RMs; BCR-RM-032 (Natural Moroccan Phosphorite), IAEA-434 (Phosphogypsum) and PRH (a local PR-RM from the Hazara Deposits of Pakistan). Instrumental Neutron Activation Analysis was used for this purpose and more than 30 elements including rare earth elements were determined in the three PR samples.  相似文献   

17.
As a new approach to rapid small-molecule analysis, surfactant-mediated matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI–TOF-MS) was successfully used in the analysis of caffeine and the vitamins riboflavin, nicotinamide, and pyridoxine in various energy drinks. Of five common MALDI matrices tested (α-cyano-4-hydroxycinnamic acid, sinapinic acid, 2,5-dihydroxybenzoic acid, dithranol, and 2′,4′,6′-trihydroxyacetophenone), α-cyano-4-hydroxycinnamic acid was found to be most suitable for analysis of high-sugar-containing energy drinks. Cetyltrimethylammonium bromide (CTAB) surfactant was used as a matrix-ion suppressor, at a matrix:surfactant mole ratio of approximately 500:1. The resulting mass spectra show very few matrix-related ions, while analyte signals were clearly observed. For comparative purposes the same analytes were identified and quantified in energy drinks by LC–ESI–MS with UV detection. Quantitatively the calibration curves of all four analytes showed a marked improvement when the surfactant-mediated method was used compared with traditional MALDI–TOF-MS; correlation coefficients of 0.989 (nicotinamide), 0.991 (pyridoxine), 0.983(caffeine) and 0.987 (riboflavin) were obtained. It was found that in quantitation of the energy drink analytes the surfactant-mediated MALDI–TOF-MS results were comparable with those from LC analysis. In reproducibility experiments RSD values ranged from 9.7 to 18.1%. The work has demonstrated that this mass spectrometric approach can be used as a rapid screening technique for fortified drinks.  相似文献   

18.
Neutron activation analysis (NAA), along with multivariate statistical methods, were employed as a proposed method to measure concentrations of some 30 elements in filtered air particulates and to estimate the fraction of particulate matter originating from crustal sources. The new method may provide a means for certain agricultural industries, in particular cattle feedlots, to take advantage of the proposed crustal credit in emission regulations, if such a credit is implemented by the US Environment Protection Agency (EPA). Chemical composition of particulate source materials as well as environmental air particulate samples were determined and results compared using computer modelling techniques to determine contribution from each potential source.  相似文献   

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20.
A liquid chromatographic method for the separation of promethazine (PR) and its positional isomer isopromethazine (IPR) is described.PR is an N-substituted phenothiazine with the actions and the uses of the antihistamines (H1-receptor antagonists). IPR is an impurity in the pharmaceutical preparations of PR and must be controlled at a level below 1%. The liquid chromatographic behaviour of PR and IPR on a hydrolytically stable β-cyclodextrin (β-CD) column with respect to mobile phase composition, pH, ionic strength and the nature of the organic modifier was also investigated. Based on the results, conditions were chosen for the isocratic separation of the two isomers. The proposed separation method is simple and rapid and permits the simultaneous determination of PR and IPR. The separation selectivity of a cyclodextrin bonded-phase column was examined. Special attention was devoted to modelling the inclusion complexes of PR and IPR with β-CD in order to predict their optimum orientation within the β-CD cavity.  相似文献   

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