三维荧光光谱的应用研究

本文通过实验探讨了三维荧光光谱在罗丹明B研究中的应用,结果显示,罗丹明B(RhB)三维荧光光谱图较二维荧光光谱图能更详细地描述被测组分的部分性质,可为综合考查罗丹明B的结构特征提供参考。文中还对近年来三维荧光光谱分析技术在不同领域中的应用做了简要介绍。     

高效毛细管电泳—电荷耦合器件检测器联用技术研究

报道了用电荷耦合检测器等设备组成的高效毛细管电泳多波长荧光检测装置。以荧光素和罗丹明Ｂ对其性能进行了评价，得到了具有光谱和时间分辨的三维电泳图，与被测物标准荧光光谱对照，可以直接鉴定被分离的化合物     

1,8-萘酰亚胺修饰的卟啉及其作为F-识别化学传感器的研究

合成了新型的具有识别F-能力的1,8-萘酰亚胺修饰的卟啉化合物,在F-的作用下,紫外-可见光谱和荧光光谱都发生明显的变化,而且可以被肉眼识别,该传感器可以作为具有F-选择性的比色和荧光化学传感器。     

功能化碳纳米管与四苯基锌卟啉轴向配位组装(英文)

本文报道了一种基于四苯基锌卟啉与含吡啶基功能化多壁碳纳米管经轴向配位组装。它们被红外光谱、拉曼光谱和透射电镜所表征。荧光光谱研究表明,在该体系中功能化多壁碳纳米管能有效地淬灭卟啉的荧光。     

不对称酰胺基二茂铁卟啉的合成及其性能研究

合成了两种不对称酰胺基二茂铁卟啉化合物,利用质谱、核磁共振氢谱、紫外光谱和红外光谱等方法表征了其结构,并讨论了酰胺基二茂铁卟啉的荧光,电化学,拉曼光谱的影响和变化规律。光谱和电化学的研究表明,取代基(CH_3O)使酰胺基二茂铁卟啉发射光谱红移,荧光量子产率增大,对于拉曼光谱的苯环振动有很大的影响;电化学结果显示使卟啉更易失电子被氧化。研究结果表明通过改变卟啉周边的取代基可以调控二茂铁卟啉的光谱和电化学性能.     

新型含稠合外环的氟硼二吡咯类碱性环境下的pH荧光探针的合成和光谱性质

制备了2种新型中位分别为对羟基苯基或苯基取代的含稠合外环的氟硼二吡咯类荧光染料。 研究了它们在极性和非极性溶剂中的吸收光谱及稳态荧光光谱性质。 采用荧光光谱滴定方法研究了它们在CH3CN-H2O溶液中对碱性环境下pH值变化的响应能力。 研究结果表明,当增加染料1溶液的碱性强度时,其荧光被显著淬灭;若将三氟乙酸滴加到该碱性溶液时,被淬灭的荧光又被恢复。 染料1在CH3CN-H2O（体积比1∶1）溶液中,其激发波长为490 nm,发射波长为540 nm,可以作为比较灵敏的碱性环境中的pH荧光探针,其pKa值为10.23。     

激光诱导荧光光谱分析进展

本文对激光诱导荧光光谱分析的研究现状和发展趋势进行了综述。着重介绍了激光诱导原子荧光光谱、激光诱导分子荧光光谱、激光低温光谱,时间分辨光谱和激光诱导荧光光谱与其它分析技术的联用研究。     

长白山白眉蝮蛇蛇毒精氨酸酯酶1的研究Ⅱ.酶学性质和荧光光谱研究(续I)

测得了长白山白眉蝮蛇毒精氨酸酯酶１的最适反应的ＰＨ范围为７．０－８．０,且与酶反应底物对甲苯磺酰－Ｌ－精氨酸甲酯的反应无明显的最适应反应温度,荧光光谱的研究结果表明：该酶的酪氨酸残基的荧光被色氨酸残基的荧光所掩盖;同步荧光光谱结果表明：当发射波长与激素波长差△λ分别为２０ｎｍ和７５ｎｍ时,精氨酸酯酶１的荧光光谱分别由酪氨酸和色氨酸残基所贡献,且处于亲水性环境中;精氨酸酯酶１的荧光发射强度受溶液酸度     

荧光和圆二色谱法用于低密度脂蛋白氧化的研究

氧化修饰低密度脂蛋白（oxidized low density lipoprotein,oxLDL）被认为是动脉硬化的关键致病因素。oxLDL的性能与其唯一的组成蛋白-载脂蛋白B-100（apoB-100）的二级结构即构象密切相关。荧光光谱和圆二色（circular dichroism,CD）光谱是研究蛋白质构象的有力手段,因此在oxLDL结构的研究中也有广泛的应用。本文综述了近年来荧光光谱和圆二色光谱在LDL氧化研究方面的应用, 并对今后的研究方向做了展望。     

基于氧化石墨烯的Ag~+与核酸中C-C碱基错配的相互作用研究

基于氧化石墨烯,以修饰了荧光分子的单链DNA为探针,利用荧光光谱和圆二色光谱研究了Ag+对双螺旋DNA中C-C碱基错配特异性识别,并建立了检测Ag+的荧光方法。荧光光谱表明Ag+能与带C-C错配碱基的双螺旋DNA作用,使原本被氧化石墨烯淬灭的标记在DNA上的荧光分子的荧光得到恢复。圆二色光谱表明Ag+能与双螺旋DNA中的C-C碱基错配相互作用,形成更稳定的C-Ag+-C结构。在最佳实验条件下,体系荧光强度的变化与Ag+的浓度在30.0~250.0 nmol/L之间呈良好的线性关系,方法检出限为19.1 nmol/L。为研究Ag+与核酸分子的相互作用及其在环境中对生物分子的影响等方面提供了重要依据。     

时间分辨激光荧光光谱分析技术研究——Ⅱ.微秒级时间分辨激光荧光光谱测量

本文主要以进行稀土元素分析为目的,在已建立的激光荧光寿命测量装置的基础上,建立了时间分辨激光荧光光谱测量装置。结合在此装置上取得的初步实验结果,对装置的原理、特点、使用范围、工作条件进行了讨论。为进行稀土元素的时间分辩激光荧光光谱分析建立了必要的手段,也为某些发光材料的发光动力学研究提供了一定的方法。     

X-ray fluorescence analysis in the ng region using total reflection of the primary beam

The article describes the experimental set-up for producing X-ray fluorescent spectra with an essentially reduced background. This is achieved by total reflection of X-rays at a plane, smooth surface of a suitable reflector material. Suprasil (quartz) and germanium are used as reflectors. Liquid samples (1–5 μl) are placed in the centre of the reflector and dried. The experimental facilities enabled the authors to attain detection limits in the ng region with energy dispersive X-ray fluorescence analysis.     

Atomic fluorescence and atomic emission measurements with an image dissector echelle spectrometer

This paper deals with the investigation of an image dissector echelle spectrometer as an analytical instrument for flame atomic fluorescence spectrometry and for flame atomic emission spectroscopy. The fluorescence was induced by high-pressure xenon arc lamps, which emitted continuum spectra and had higher power ratings, i.e. 1.6 and 2.5 kW, than those normally used for the same purpose. The experimental set-up included two different types of premix burners and one type of total consumption burner. A spherical reflector was applied to improve the utilization of the fluorescence radiation. Two different coatings were tested. None gave the expected enhancement.Detection limits and growth curves were measured for 8 different elements (Ca, Co, Cu, Fe, K, Mg, Na and Ni) in a non-separated air/acetylene flame. The attained detection limits were found to be equally good or somewhat better in flame atomic fluorescence excited with continuum sources than previously reported in the literature, i.e. using similar flames. In flame atomic emission spectroscopy better detection limits have been reported before.     

Detection of trace amounts of Ni by laser-induced fluorescence in graphite furnace with intensified charge coupled device

A laser-induced fluorescence in graphite furnace (LIF-GF) set-up has been equipped with an intensified CCD detector (ICCD) that enables simultaneous multichannel detection of large wavelength regions. The main advantages of such a system in comparison with conventional photomultiplier detection are: simultaneous detection of several fluorescence wavelengths for easy characterization of excitation and fluorescence spectra and for an increase of the absolute sensitivity and spectral selectivity; simultaneous monitoring of background signals, such as those due to matrix interferences, blackbody radiation and scattered laser light; decrease of the susceptibility to radio-frequency pick-ups emitted from the pump laser due to the delayed read-out procedure; time-resolved studies of fluorescence spectra for improved elemental selectivity or for studies of atomization processes, and a possibility to perform two-dimensional imaging of height distributions of atomization and, in combination with an imaging spectrometer, diffusion processes in the furnace. The first work on LIF-GF with ICCD detection has been performed on Ni. The most sensitive and versatile excitation and detection wavelengths have been identified. Detection limits in water solutions by the LIF-GF technique have been improved by two orders of magnitude and are found to be 0.015 pg with ICCD and 0.01 pg using a photomultiplier at the most sensitive excitation and detection wavelengths. Nickel in concentrations has been detected in aqueous standard reference samples with sodium concentrations ranging from to % (riverine water and estuarine water) with good accuracy and precision. The Ni contents of standard riverine and estuarine water were determined to 1.00 ± 0.11 and 0.75 ± 0.07 ng/ml, respectively. The certified concentrations are 1.03 ± 0.10 and 0.743 ± 0.078 .     

Detection of gaseous organic compounds by their infra-red emission stimulated by a laser beam

Excitation by a carbon dioxide laser has been used to obtain the infra-red emission spectra of a number of gaseous aliphatic hydrocarbons. The experimental set-up is described and the spectra obtained are correlated with bond vibrations and are shown to be similar to infra-red absorption spectra. The future requirements of this technique are discussed and its analytical possibilities are indicated.     

Fluorescence lifetime measurements of pseudoazulenes using picosecond-resolved single photon counting

The very rapidly decaying fluorescence of three different pseudoazulenes was measured, enabling to check simultaneously the time resolution and accuracy of the set-up with mode-locked and synchronously pumped lasers and time correlated single photon counting. Instrumental details are given to reach optimum time resolution and accuracy. The fluorescence decay of a widely used fluorescent standard was used in the deconvolution procedure.     

Instrumentation for ac four-probe measurements of large impedances

An ac four-probe set-up was constructed to measure impedance spectra between 1 Hz and 100 kHz on solid electrolytes up to impedance values of 10 GΩ. The influence of the system on the measurement was analysed for the previous and the new set-up. A solution to minimise the source of errors by pre-amplifiers for current and voltage signals is presented. A new sample holder was constructed for best results in conjunction with the new pre-amplifiers. The holder is designed for measurements on solid electrolytes over a wide temperature range. The performance of the improved set-up and the residual error sources are discussed. Measurements of impedance spectra on YSZ and BICUVOX.10 in a four-probe arrangement are given as an example.     

Performance of fluorescence correlation spectroscopy for measuring diffusion and concentration.

Fluorescence correlation spectroscopy (FCS) has become an important tool for measuring diffusion, concentration, and molecular interactions of cellular components. The interpretation of FCS data critically depends on the measurement set-up. Here, we present a rigorous theory of FCS based on exact wave-optical calculations. Six of the most important optical and photophysical factors that influence FCS are studied: fluorescence anisotropy, cover-slide thickness, refractive index of the sample, laser-beam geometry, optical saturation, and pinhole adjustment. Our theoretical framework represents a general attempt to link all relevant parameters of the experimental set-up with the measured correlation function.     

高效毛细管电泳-电荷耦合器件检测器联用技术研究Ⅱ.混合荧光染料分离

使用电荷耦合器件荧光检测毛细管电泳装置，在13min内基线分离了混合荧光染料的5个组份。详细研究了pH、电泳电压、曝光时间等多种因素对混合物电泳分离和荧光信号强度的影响。从具有光谱和时间分辨的三维电泳图上，可以直接鉴定和测量被分离的化合物。     

铜(Ⅱ)离子与神经红蛋白的相互作用

利用紫外可见吸收光谱、荧光光谱、同步荧光光谱及圆二色(CD)光谱研究了铜髤离子与神经红蛋白(NGB)的相互作用。结果表明,Cu2+离子使NGB在280nm处的紫外吸收增强,说明Cu2+与NGB发生了相互作用;Cu2+使NGB内源性荧光发生猝灭,其猝灭机制为静态猝灭;同步荧光光谱表明,Cu2+使色氨酸微环境的疏水性有所降低,Cu2+对NGB的作用位点更接近于色氨酸;CD光谱显示Cu2+没有引起NGB二级结构明显的变化。