共查询到20条相似文献,搜索用时 3 毫秒
1.
Synthesis of 3-Deoxy-d-threopentofuranose 5-Phosphate,a Substrate of Arabinose 5-Phosphate Isomerase
3-Deoxy-d-threopentofuranose 5-phosphate, a substrate of arabinose 5-phosphate isomerase, has been synthesised starting from d-arabinose. Selective protection of the hydroxyl groups at C-1, C-2, and C-5 allowed deoxygenation of position 3 by conversion into a thiocarbamate and radical reduction. Deprotection and phosphorylation of the primary hydroxyl group and final deprotection of the other hydroxyl groups afforded the desired compound. 相似文献
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Synthesis of Protein A Conjugates with Silver Nanoparticles 总被引:1,自引:0,他引:1
The single-stage synthesis of the conjugates of protein A with silver nanoparticles is performed. The dependence of the dispersity of the prepared sols on the composition of reaction mixture is investigated by optical spectroscopy and transmission electron microscopy. The relative stability of the obtained preparations with respect to the coagulation by NaCl is estimated. 相似文献
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蛋白质高分子结合体是蛋白质与高分子化合物以特定位置或方式结合的产物。其中,蛋白质(包括酶和多肽)分子中氨基酸残基上的氨基、巯基和羧基是常用的结合位点。本文主要对蛋白质高分子结合体的制备方法进行了综述。聚乙二醇是合成高分子中能够有效改善蛋白质性能的修饰剂,而多糖则是用于制备蛋白质高分子结合体较成功的天然高分子化合物。“点击化学”、活性聚合技术等技术已经被成功应用于蛋白质高分子结合体的制备。某些具有特异结合功能基团的化合物(如金属卟啉、生物素等)与高分子共价结合后也可制备蛋白质高分子结合体。在研究蛋白质高分子结合体制备方法的基础上,近年来开始了这类大分子的自组装行为研究,尤其是对巨型双亲性分子自组装行为的研究,这为设计和构筑先进功能材料提供了新的思路。与高分子化合物的结合是改善蛋白质性能和拓宽蛋白质应用范围的重要技术之一。蛋白质高分子结合体不但可用于生物医药领域,而且在纳米技术和材料科学等领域具有潜在的优势。 相似文献
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同位素标记及未标记的1-脱氧-D-木酮糖-5-磷酸和2-甲基-D-赤藓糖醇-4-磷酸的合成方法 总被引:1,自引:0,他引:1
萜类化合物构成了最大的一个天然产物家族,结构复杂多变,且有许多重要的生理活性.2-甲基-D-赤藓糖醇4-磷酸(MEP)途径是近年来发现并建立的一条萜类化合物的生物合成途径,其中所涉及到的酶均可作为靶标来进行新抗菌素的筛选.综述了以化学合成及酶催化合成方法制备MEP途径中关键中间体1-脱氧-D-木酮糖5-磷酸和2-甲基-D-赤藓糖醇4-磷酸的进展,并着重介绍了同位素标记的这两个化合物的制备方法. 相似文献
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Valino AL Palazzolo MA Iribarren AM Lewkowicz E 《Applied biochemistry and biotechnology》2012,166(2):300-308
2-Deoxyribose 5-phosphate (DR5P) is a key intermediate in the biocatalyzed preparation of deoxyribonucleosides. Therefore,
DR5P production by means of simpler, cleaner, and economic pathways becomes highly interesting. One strategy involves the
use of bacterial whole cells containing DR5P aldolase as biocatalyst for the aldol addition between acetaldehyde and d-glyceraldehyde 3-phosphate or glycolytic intermediates that in situ generate the acceptor substrate. In this work, diverse
microorganisms capable of synthesizing DR5P were selected by screening several bacteria genera. In particular, Erwinia carotovora ATCC 33260 was identified as a new biocatalyst that afforded 14.1-mM DR5P starting from a cheap raw material like glucose. 相似文献
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在生命活动中起重要作用的蛋白质和金属卟啉是典型的生物高分子和金属配合物,而且绝大部分蛋白质与金属卟啉是通过形成结合体共同发生作用的,因此,研究天然金属卟啉蛋白质结合体的结构与功能并进行人工模拟受到关注并取得了很大进展。本文在对蛋白质与金属卟啉的结构与类型进行简单介绍的基础上,综述了 金属卟啉与蛋白质的天然结合体,如细胞色素P-450、过氧化物酶、血红蛋白、肌红蛋白及脑红蛋白等。总结了金属卟啉与蛋白的人工结合体,如原卟啉、血卟啉及其衍生物与血清白蛋白的结合体;合成水溶性与不溶性金属卟啉与白蛋白的结合体。介绍了人工合成蛋白,即基因重组蛋白与合成金属卟啉的结合体,如用做人工合成血液的栏式铁卟啉白蛋白结合体。到目前为止,不但金属卟啉可以合成,而且白蛋白、血红蛋白也可以通过基因重组进行人工合成。金属卟啉蛋白质结合体已应用于制备人工血液、疾病检测、治疗,及光解水产氢等多个领域。 相似文献
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Preet Kamal Kaur Jakka S. Supin S. Rashmi Sushma Singh 《Applied biochemistry and biotechnology》2014,173(7):1870-1884
Ribose-5-phosphate isomerase B (RpiB), a crucial enzyme of pentose phosphate pathway, was proposed to be a potential drug target for visceral leishmaniasis. In this study, we have analyzed the biophysical properties of Leishmania donovani RpiB (LdRpiB) enzyme to gain insight into its unfolding pathway under various chemical and thermal denaturation conditions by using fluorescence and CD spectroscopy. LdRpiB inactivation precedes the structural transition at lower concentrations of both urea and guanidine hydrochloride (GdHCl). 8-Anilinonapthalene 1-sulfonic (ANS) binding experiments revealed the presence of molten globule intermediate at 1.5 M GdHCl and a nonnative intermediate state at 6-M urea concentration. Acrylamide quenching experiments further validated the above findings, as solvent accessibility of tryptophan residues increased with increase in GdHCl and urea concentration. The recombinant LdRpiB was completely unfolded at 6 M GdHCl, whereas the enzyme molecule was resistant to complete unfolding even at 8-M urea concentration. The GdHCl- and urea-mediated unfolding involves a three-state transition process. Thermal-induced denaturation revealed complete loss of enzyme activity at 65 °C with only 20 % secondary structure loss. The formation of the well-ordered β-sheet structures of amyloid fibrils was observed after 55 °C which increased linearly till 85 °C as detected by thioflavin T dye. This study depicts the stability of the enzyme in the presence of chemical and thermal denaturants and stability-activity relationship of the enzyme. The presence of the intermediate states may have major implications in the way the enzyme binds to its natural ligand under various conditions. Also, the present study provides insights into the properties of intermediate entities of this important enzyme. 相似文献
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Peptidic model conjugates carrying a single 2-carboxy-4,6-dinitrophenyl haptenic group, and as carbohydrate moieties D -gluconoyl, β-D -mannopyranosyl, 2-deoxy-β-D -glucopyranos-2-yl, or lactobionoyl residues, including the pseudocarbohydrate residue 1,3,4,5-tetrahydroxycyclohexane-1-carbonyl, were synthesized. Conjugates carrying the lactobionoyl of the bis(2-deoxy-β-D -glucopyranos-2-yl) moiety were anaphylactogenic in the guinea pig, passively sensitized against 2-carboxy-4,6-dinitrophenyl antigen. 相似文献
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尿苷环甘油磷脂缀合物的合成 总被引:1,自引:0,他引:1
报道尿苷2-烷基(酰基)-1,3-甘油硫代环磷酸酯的合成,用碘活化的六乙基亚磷酰三胺作为磷酸化试剂,分离得到了硫代环磷酸酯的两个顺反异构体。初步生物活性测试结果表明,该化合物具有一定的细胞毒性。 相似文献
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报道了甘油脂与Germatranes缀合物的合成,合成中以DCC为缩合剂,以吡啶为催化剂,经缩合反应得到目标产物,对缩合方法和反应条件进行了探讨,化合物结构经HNMR、IR和元素分析确证。 相似文献
14.
Liu Hua XU Hu ZHENG Ling Ling WENG 《中国化学快报》2005,16(11):1434-1436
Bone tumor, as a common disease, is treated by surgical resection, radiotherapy and chemotherapy. Chemotherapy is one of the most important treatment, however, a major problem of chemotherapy is lack of selectivity of cytotoxic drugs. Although many attempts have been made to increase the selectivity of therapeutic drugs for the bone diseases, such as osteoporosis, paget’s, hypercalcemia and bone metastases by conjugating them with targeting carriers1-4, there are still no bone-targeting agent… 相似文献
15.
《Analytical letters》2012,45(12):2025-2038
Abstract A capacitive immunosensor for the detection of the interaction between benzo[a]pyrene-bovine serum albumin (BaP-BSA) and a monoclonal antibody (designated as MAb10c10) specific to BaP has been developed. MAb10c10 was immobilized on a gold electrode through a self-assembled monolayer of cystamine. Linear sweep voltammetry showed that the charging current decreased with BaP-BSA binding to the antibody on the electrode, and calibration curves were constructed for BaP-BSA and Pyrene-BSA in the range of 0.01 μM-6.00 μM. Selectivity over structurally related analytes is observed. 相似文献
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以4,4’-二羟基二苯甲酮为原料, 通过McMurry反应合成了三种环芬尼类化合物. 优化条件实现两个酚羟基选择性生成单ω-溴代烷基醚, 通过亲核取代反应连接三齿螯合剂, 得到了一系列雌激素受体β分子探针前体. 用[Et4N]2[Re(CO)3Br3]与之配位合成了其冷标记配合物, 为SPECT (single photon emission computed tomography)显像雌激素受体β从而评估乳腺癌的恶化程度奠定了基础. 产物通过IR, 1H NMR, 13C NMR, HRMS或元素分析进行了表征. 相似文献
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YiChunZHANG YingXiaLI HuaShiGUAN 《中国化学快报》2005,16(2):179-182
Two series of indomethacin conjugates with D-glucosamine were prepared with the objectives of reducing ulcerogenic potency, increasing the bioavailability of indomethacin and exerting the coordinative effects on osteoarthritis. The structures of the conjugates were identified by ^1H NMR and ^13C NMR. The ester conjugates inhibited edema as potent as indomethacin. 相似文献
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本文合成了两种三联吡啶修饰的萘酰亚胺化合物NPI1和NPI2,并利用紫外-可见吸收光谱(UV-Vis)、圆二色光谱(CD)、荧光共振能量转移(FRET)等方法研究了它们与双链CT DNA和Htelo G-四链体DNA的相互作用。实验结果表明,化合物NPI1和NPI2对G-四链体DNA具有很好的结合能力和选择性,溶液中的碱金属离子种类和萘酰亚胺基团上的取代基对NPI1和NPI2与DNA的作用有很大的影响。在含K+的缓冲液中,NPI2与G-四链体的结合常数达到1.06×108 L/mol,是与双链CT DNA结合常数的268倍。圆二色谱结果表明在不含碱金属离子的溶液中,NPI1和NPI2可诱导Htelo DNA形成反平行结构G-四链体。Autodock分子对接模拟表明NPI1和NPI2可以通过堆积作用、静电作用、氢键等作用方式与G-四链体结合,使得它们对G-四链体具有很高亲和性(Ka>107 L/mol)。 相似文献
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以碘活化的六乙基亚磷酰三胺为磷酰化及环化试剂,经一锅法合成并分离得到硫代环磷酸酯的两个非对映异构体,并对这两个非对映异构体的性质进行了研究. 相似文献