胶束电动毛细管色谱法测定穿心莲中穿心莲内酯和脱水穿心莲内酯

采用胶束电动毛细管色谱法分离测定了中药穿心莲中穿心莲内酯和脱水穿心莲内酯。电泳条件 :以2 0mmol L磷酸缓冲溶液 1 0mmol LSDS(含 5 %甲醇 ,V/V ,pH 6.8)为电泳介质 ,未涂层石英毛细管 (75 μmi.d.× 5 0cm ,有效分离长度 4 2 .2cm)为分离通道 ,压力进样 (2 5 0kPa·s) ,2 0kV恒压电泳 (2 5℃ )分离 ,检测波长 2 4 0nm。在 1 0 .4～93 .5mg/L ,和 1 0 3～ 92 .7mg L范围内 ,对两种内酯分别进行了定量分析。加样回收率穿心莲内酯为 1 0 2 % ,脱水穿心莲内酯为 1 0 2 %。     

胶束毛细管电泳在线扫集技术同时分离测定穿心莲内酯和脱水穿心莲内酯的研究

采用胶束毛细管电泳在线扫集技术分离测定了中药穿心莲中脱水穿心莲内酯和穿心莲内酯.电泳条件:以20 mmol/L H3BO310 mmol/L NaH2PO4-50mmol/L SDS(含体积分数20%甲醇,pH 2.4)为电泳运行缓冲溶液,未涂层石英毛细管(58 cm×50 μm i.d.,有效长度为41.2 cm)为分离通道,重力进样,进样高度为11 cm,-20 kV恒压,检测波长为246 nm.富集倍数可以达到200倍以上.在5.70～91.20 mg/L,和3.96～31.68 mg/L范围内呈良好的线性关系,对两种内酯分别进行了定量分析.加标平均回收率脱水穿心莲内酯为100.80%,穿心莲内酯为98.06%.     

在线扫集-胶束毛细管电动色谱法测定金感胶囊中的绿原酸、脱水穿心莲内酯和马来酸氯苯那敏

建立了在线扫集-胶束毛细管电动色谱法(Sweeping-MEKC)测定金感胶囊中的绿原酸、脱水穿心莲内酯和马来酸氯苯那敏的新方法.考察了pH值、四硼酸钠浓度、硼酸浓度、十二烷基硫酸钠(SDS)浓度、电压、有机溶剂和进样时间对分离效果的影响.以12 mmol/L四硼酸钠-50 mmol/L硼酸-50 mmol/L SDS...     

微乳液组成对毛细管微乳液电动色谱的影响

研究了一组电中性芳香族化合物的毛细管微孔液电动色谱分离，考察分离介质微乳液的组成对电动色谱保留时间的影响，实验结果表明，各芳香化俣物的保留时间均随表面活性剂２增加而增长；内相和助表面活性剂对有，无亲水基团入香化合物的保留时间有不同的影响规律，选用微乳液组成为８０ｍｍｏｌ／Ｌ正庚烷－１２０ｍｍｏｌ／ＬＳＤＳ－９００ｍｍｏｌ／Ｌ正丁醇－１０ｍｍｏｌ／Ｌ硼砂，样品在１４ｍｉｎ内达到完全分离，理论板数为３     

蛋白质的微乳液毛细管电动色谱分离研究

考察了用微乳液毛细管电动色谱(MEEKC)分离蛋白质时微乳液组成等不同因素对分离的影响,并与胶束电动色谱进行对比,探讨了其分离机理,为蛋白质的分离鉴定提供了一种有力的工具.     

微乳液毛细管电动色谱应用进展

微乳液毛细管电动色谱（MEEKC）是在胶束电动色谱基础上发展起来的一种电动色谱新技术。近几年来,MEEKC在各个领域的应用又有了长足的进步,尤其是在维生素、药物、天然产物、手性分离、生物分子、环境分析,以及药物疏水性评价上呈现出强势的发展,本文针对这些领域的MEEKC方法进行了评述。     

微乳液毛细管电动色谱烷基苯的保留行为

以阴离子表面活性剂十二烷基硫酸纳（SDS）和阳离子表面活性剂十六烷基三甲基溴化铵（CTMAB）组成的微乳液体系为分离介质,研究一组烷基苯同系物的毛细管电动色谱分离,较系统地考察了多种微乳液条件下溶质的保留时间,容量因子和电动色谱时间窗。结果表明,在SDS组成的微乳液体系中,烷基苯同系物的logk′与烷基链碳数(C=0～5)呈良好线性关系,logk′=aC+b,r＞0.9921。微乳液组成的变化对两种体系的电动分离有不同的影响规律,并对作用机理进行了探讨。     

微乳液毛细管电动色谱法测定大鼠滑膜细胞中雷公藤甲素的含量

建立微乳液毛细管电动色谱法快速测定大鼠滑膜细胞内外液中雷公藤甲素含量的方法.在雷公藤甲素不同给药时间孵育条件下,将滑膜细胞分为空白对照组和给药组,测定滑膜细胞内外液中雷公藤甲素含量,并比较了细胞外液中雷公藤甲素在不同给药时间的含量变化.毛细管电泳运行缓冲液组成:1％(w/V)SDS,3％(V/V)正丁醇,1％(V/V)乙酸乙酯,96％(V/V)5 mmol/L硼砂-10 mmol/L磷酸盐溶液,pH 9.0;运行电压:25 kV;压力进样:0.5 psi×6 s;电泳操作温度:25℃;检测波长:214 nm.结果表明,细胞内外液中的雷公藤甲素所呈现的线性关系良好,相关系数分别为0.9995和0.9991.当给药24h时,细胞内外液中的雷公藤甲素浓度分别为0.736和20.745 μg/mL.本方法简单准确、灵敏度高、精密度好,可用于滑膜细胞内外液中雷公藤甲素浓度的动态变化规律研究,为进一步研究中药有效成分对滑膜细胞功能和活性的影响提供方法学基础.     

微乳毛细管电动色谱快速分析脂溶性维生素

建立了一种新的微乳体系,成功地应用于微乳毛细管电动色谱(MEEKC)快速分析脂溶性维生素VA、VD3和VE.本微乳液的组成为:1.2%(m/m)十二烷基硫酸钠(SDS)-21%(V/V)正丁醇-18%(V/V )乙腈-0.8%(m/m)正己烷-20 mmol/L H3BO3-Na2B4O7缓冲液( pH 8.4 ).该微乳体系中,助表面活性剂正丁醇和有机溶剂乙腈对脂溶性维生素的分离起到了重要的作用.当分离电压为25 kV,柱温为25 ℃时,VA、VD3和VE在13 min内达到基线分离.3种脂溶性维生素的迁移时间和峰面积的RSD(n= 5)<2.5%和4.5%;VA、VD3和VE分别在20～1000、5～1000和5～1000 mg/L范围内与峰面积呈线性关系;检出限(S/N=3)分别为12、 0.72和0.29 mg/L.本体系应用于市售VE胶囊的测定,结果与标示值相符.     

微乳液毛细管电动色谱快速分析解热镇痛药中的有效成分

建立了微乳液毛细管电动色谱快速测定解热镇痛药中非那西丁、氨基比林和咖啡因的新方法。采用由乙酸乙酯-十二烷基硫酸钠(SDS)-正丁醇-硼砂缓冲液组成的微乳液体系,以氯霉素为内标,3种有效成分在2.5 min内完成分离,峰面积相对标准偏差(RSD)在1.2%～1.6%之间,回收率在95.6%～104.0%之间。实验考察了缓冲溶液的浓度、pH值、SDS浓度以及助表面活性剂的种类、含量对分离测定的影响。该法可用于实际样品分析。     

Separation of Felted Explosives by Pressurized Microemulsion Electrokinetic Chromatography

A new MEEKC method assisted with pressure-driven mobile phase was presented for the separation of felted explosives. Microemulsion solution was composed of 80 mmol/L heptane-120 mmol/L SDS (sodium dodecyl sulphate)-900 mmol/L butanol-10 mmol/L borate at pH 9.4 and a pressure-driven flow of 0.020 mL/min under 1.3 MPa was employed to manipulate the separation. Explosives HMX (1,3,5,7-tetranitro-1,3,5,7-tetrazacyclooctane) and TATB(triamino-trinitrobenzene), which were felted on fluorine rubber F2311 (polytrifluorochlorethylene and polyvinylidene fluoride 1/1 co-polymerization) and F2314 (polytrifluorochlorethylene and polyvinylidene fluoride 4/1 co-polymerization) were well separated with very good peak shapes.     

Chiral Separations by Ligand-Exchange Micellar Electrokinetic

The separation of enantiomers is of great importance in biology, pharmaceutics, agriculture and environment. The different separation modes ( i. e. capillary zone electrophoresis ( CZE ), micellar electrokinetic chromatography ( MEKC ), capillary electrochromatography ( CEC ), etc )     

毛细管微乳液电动色谱的原理及应用

综述了近年来毛细管微乳液电动色谱(MEEKC)的研究进展。对MEEKC的分离原理进行了阐述;对微乳液的组成和其他影响MEEKC分离的因素进行了总结;对MEEKC在各个领域的应用作以分类评述;并将MEEKC和胶束电动毛细管色谱(MEKC)予以比较。     

Optimized Separation of Nine Xanthones by Microemulsion Electrokinetic Chromatography

A microemulsion electrokinetic chromatography method has been firstly used for the separation of the therapeutically important xanthones from Securidaca inappendiculata. The separation of the nine xanthones was systematically optimized with respect to pH, composition of microemulsion,addition of cyclodextrins, applied voltage and column temperature. Baseline separation was successfully achieved for the nine xanthones, which was also compared with that by micellar electrokinetic chromtography.     

Separation of Six Pyridoncarboylxic Acid Derivatives by Micellar and Microemulsion Electrokinetic Chromatography

Micellar electrokinetic chromatography (MEKC) and microemulsion electrokinetic chromatography (MEEKC) are two kinds of electrokinetic capillary chromatography (EKC), which are characterized of high solubilization capacity and separation efficiency. In our previous work, some polar organic compounds and hydrophobic neutral compounds were separated successfully by EKC1-3. In this paper, these methods were used for separating six pyridoncarboylxic acid derivatives with similar structures. T…     

Simultaneous Determination of Heroin, Amphetamine and their Basic Impurities and Adulterants Using Microemulsion Electrokinetic Chromatography

Heroin and amphetamine are two most abused drugs that can be highly complex, containing various impurities, byproducts, adulterants and diluents due to differences in the agricultural and manufacturing procedures. Therefore, the comprehensive analysis of above illicit drugs seized or purchased undercover by law enforcement authorities is important for legal and intelligence gathering purposes, and clinical and pharmaceutical purposes as well1. In last decade, micellar electrokinetic chromatogr…