In situ synthesis of ceria nanoparticles in the ordered mesoporous carbon as a novel electrochemical sensor for the determination of hydrazine

A novel ceria (CeO₂)–ordered mesoporous carbon (OMC) modified electrode for the sensitive amperometric determination of hydrazine was reported. CeO₂–OMC composites were synthesized via a hydrothermal method at a relatively low temperature (180 °C) and characterized by scanning electron microscopy (SEM), transmission electron microcopy (TEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD). The CeO₂–OMC modified glassy carbon electrode was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) and indicated good electrocatalytic effect to the oxidation of hydrazine. Under the optimized conditions, the present sensor could be used to measure hydrazine in wide linear range from 40 nM to 192 μM (R² = 0.999) with a low detection limit of 12 nM (S/N = 3). Additionally, the sensor has been successfully applied to detect hydrazine in real water samples and the recoveries were between 98.2% and 105.6%. Eventually, the sensor exhibited an excellent stability and reproducibility as a promising method for determination of hydrazine.     

有序中孔炭的制备及电性能研究

采用微湿含浸法制备了一系列具有不同比表面积和孔径分布的超级电容器有序中孔炭材料,同时采用Al-SBA-15为模板剂制得具有六方排列的空心炭管CMK-5.所制得的有序中孔炭的BET比表面积随糠醇加入量的增加而减小.电化学性能测试结果表明,在1mA·cm-2的充放电电流密度下各中孔炭材料比电容的大小顺序与其BET比表面积的大小顺序相一致.在所有样品中AlSC-0.8由于具有最大的比表面积,因此其比电容最大,达87.8F·g-1.倍率性能测试结果表明,具有CMK-3结构的SC-2.0的倍率性能最好,在50mA·cm-2的放电电流密度下其放电比电容接近AlSC-0.8有序中孔炭的水平.     

有序介孔碳CMK-3负载金纳米微粒复合材料的制备和表征

分别采用等体积浸渍-甲醛还原、等体积浸渍-氢气还原及溶胶负载法在介孔碳CMK-3上负载金纳米微粒;利用透射电镜和粉末X射线衍射仪对比分析了采用3种方法得到的复合材料的微结构和相组成;并测定了采用溶胶负载法得到的不同金含量的复合材料的热稳定性.结果表明,所制备的金纳米微粒的尺寸因制备方法不同而呈现明显差异;负载于复合材料中的金纳米微粒具有很好的热稳定性.     

Palladium nanoparticle decorated carbon ionic liquid electrode for highly efficient electrocatalytic oxidation and determination of hydrazine

In this work arrays of palladium nanoparticles were synthesized on carbon ionic liquid electrode (CILE) (Pd/CILE), and the electrocatalytic oxidation of hydrazine was investigated using this electrode. Electrochemical oxidation of hydrazine in phosphate buffer (pH 7) was performed using cyclic voltammetry and square wave voltammetric techniques (SWV). Using the proposed electrode, a highly reproducible and well-defined peak was obtained for hydrazine at a very low potential of −0.02 V versus Ag/AgCl. A linear dynamic range of 5-800 μM with an experimental detection limit of 0.82 μM was obtained. These results show that the proposed electrode displays better electrocatalytic activity compared to the previously reported palladium modified electrodes towards oxidation of hydrazine.     

阿拉伯糖基有序介孔碳修饰电极检测盐酸奈福泮的研究

以阿拉伯糖为碳源,介孔硅(SBA-15)为模板剂,用硬模板法制备有序介孔碳材料,采用场发射扫描电镜(Scanning Electron Microscopy,SEM)、透射电子显微镜(Transmission Electron Microscope,TEM)、全自动比表面及孔隙度分析仪(Brunner Emmet Te...     

含氮前驱体对氮杂有序介孔炭材料及其氧还原电催化性能的影响

燃料电池中贵金属铂的大量使用是阻碍其发展的关键因素,亟需探索高效廉价的替代型电催化剂.在目前的替代型非贵金属催化剂研究中,氮杂炭材料是一类氧还原反应催化活性最好、成本最低廉的催化剂,被认为是最有可能取代Pt催化剂而获得实际应用的催化剂.氮杂有序介孔炭材料因具有极高的比表面积和规整的孔道结构,可实现活性位点的密集组装与反应物料的快速传输,受到研究者的广泛关注.本文分别以苯胺、吡咯和邻菲罗啉为含氮前驱体,介孔分子筛SBA-15为硬模板,采用纳米浇铸法成功制备了具有高比表面积的氮杂有序介孔炭材料,系统研究了不同含氮前驱体对氮杂有序介孔炭材料的影响.采用氮气吸附-脱附等温线、透射电子显微镜(TEM)、X射线衍射(XRD)和X射线光电子能谱(XPS)等方法研究了氮杂有序介孔炭的组成与结构,采用循环伏安法(CV)以及线性扫描伏安法(LSV)等手段考察了其电化学行为与氧还原反应极化性能.氮气吸附-脱附等温线结果表明,采用三种不同含氮前驱体制备的氮杂炭材料都对应Ⅳ型吸脱附等温线以及H4型滞后环,表明所制备的氮杂炭材料具有介孔结构.由TEM可以清楚地观察到氮杂炭材料已经成功地反转了SBA-15模板的孔道结构.同时发现,含氮前驱体对氮杂炭材料的比表面积和孔结构产生较大影响:以吡咯和邻菲罗啉为前驱体制备的炭材料C-PY-900和C-Phen-900的比表面积较高,分别为765和746 m2/g,而以苯胺为前驱体制备的炭材料C-PA-900比表面积较小(569 m2/g);三种炭材料平均孔径大小顺序为C-Phen-900 (3.7 nm)＜ C-PY-900 (5.0 nm)＜ C-PA-900 (5.9 nm),这是由于不同含氮前驱体在高温焙烧过程中热分解行为不同所致.XRD结果发现,含氮前驱体对氮杂炭材料的晶型基本没有影响,均为无定形碳.XPS结果表明,采用苯胺、吡咯以及林菲啰啉为前驱体制备的氮杂炭材料中氮含量基本相同,分别为3.13 at％,3.32 at％和3.33 at％,说明在相同热解条件下材料中的氮含量基本不受前驱体的影响,但不同配位环境的氮含量以及氮活化碳原子的含量却有较大差异,其氮活化碳原子的相对含量分别为15.60％,19.87％和23.04％.电化学测试结果表明,三种氮杂介孔炭材料的氧还原反应电催化活性顺序为C-PA-900＜C-PY-900＜C-Phen-900,其H2O2产率低于30％,说明氧还原反应经历4电子转移路径.在碱性条件下,所制氮杂有序介孔炭材料C-PY-900和C-Phen-900表现出较商品Pt/C催化剂更加优异的氧还原反应电催化性能.综上可见,通过改变含氮前驱体的类型可以有效调变氮杂炭材料的比表面积、孔道结构以及N 1s与C 1s化学态,从而调控氧还原反应活性,本文不仅制备出高活性的非贵金属氧还原电催化剂,同时也为高活性炭基电催化剂的可控制备提供了思路.     

基于有序介孔碳的氯离子选择性电极的制备及其在手持传感系统中的应用

以有序介孔碳(OMC)球为离子-电子转换层,制备了固态氯离子选择性电极,构建了基于离子敏感的场效应晶体管(ISFET)的手持式传感系统,用于检测人体血清中的氯离子。优化了OMC前驱体的碳化温度,探究了OMC形貌结构对电极传感性能的影响;电极柔性化制备后考察了其在手持系统中对氯离子的检测效果。结果表明,最优条件下,电极在5.12×10^-4~1.02 mol/L的浓度范围呈现线性响应,响应斜率为60 mV/decade。该柔性电极在手持传感系统中展现出高灵敏度和重现性,可用于人体血清样品中氯离子的检测,其回收率为96.3%~104.9%。     

A sensitive amperometric sensor for hydrazine and hydrogen peroxide based on palladium nanoparticles/onion-like mesoporous carbon vesicle

Onion-like mesoporous carbon vesicle (MCV) with multilayer lamellar structure was synthesized by a simply aqueous emulsion co-assembly approach. Palladium (Pd) nanoparticles were deposited on the MCV matrix (Pd/MCV) by chemical reduction of H₂PdCl₄ with NaBH₄ in aqueous media. Pd(X)/MCV (X wt.% indicates the Pd loading amount) nanocomposites with different Pd loading amount were obtained by adjusting the ratio of precursors. The particular structure of the MCV results in efficient mass transport and the onion-like layers of MCV allows for the obtainment of highly dispersed Pd nanoparticles. The introduction of Pd nanoparticles on the MCV matrix facilitates hydrazine oxidation at more negative potential and delivers higher oxidation current in comparison with MCV. A linear range from 2.0 × 10⁻⁸ to 7.1 × 10⁻⁵ M and a low detection limit of 14.9 nM for hydrazine are obtained at Pd(25)/MCV nanocomposite modified glassy carbon (GC) electrode. A nonenzymatic amperometric sensor for hydrogen peroxide based on the Pd(25)/MCV nanocomposite modified GC electrode is also developed. Compared with MCV modified GC electrode, the Pd(25)/MCV nanocomposite modified GC electrode displays enhanced amperometric responses towards hydrogen peroxide and gives a linear range from 1.0 × 10⁻⁷ to 6.1 × 10⁻³ M. The Pd(25)/MCV nanocomposite modified GC electrode achieves 95% of the steady-current for hydrogen peroxide within 1 s. The combination of the unique properties of Pd nanoparticles and the porous mesostructure of MCV matrix guarantees the improved analytical performance for hydrazine and hydrogen peroxide.     

Arsenic sorption and redox transformation on iron‐impregnated ordered mesoporous carbon

Ordered mesoporous carbon has been actively investigated for its potential applications as catalyst supports, electrochemical materials and gas separation media. In this study, we tested an iron‐modified ordered mesoporous carbon (FeOMC) for its ability to adsorb arsenic from the aqueous phase. The FeOMC synthesis involved the preparation of an ordered silica template SBA‐15, in situ polymerization of acrylic acid in the template, carbonization and template removal to obtain the ordered mesoporous carbon, and iron impregnation. Batch experiments showed that the pH level of the solution had a major impact on arsenic sorption. Further, we found that the presence of anions (i.e. PO₄^3? and SiO₃^2?) could significantly decrease the sorption of both arsenate and arsenite. Arsenite oxidation to arsenate was observed in alkaline solutions, with or without anions being present. The oxidation of arsenite was attributed to both direct and catalytic reactions with the surface functional groups on the ordered mesoporous carbon. Adsorption of arsenic on FeOMC could be well explained by the surface complexation model. Copyright © 2007 John Wiley & Sons, Ltd.     

Synthesis of a new ordered mesoporous NiMoO4 complex oxide and its efficient catalytic performance for oxidative dehydrogenation of propane

Highly ordered mesoporous NiMoO4material was successfully synthesized using mesoporous silica KIT-6 as hard template via vacuum nanocasting method. The structure was characterized by means of XRD, TEM, N2adsorption-desorption, Raman and FT-IR. The mesoporous NiMoO4with the coexistence of α-NiMoO4and β-NiMoO4showed well-ordered mesoporous structure, a bimodal pore size distribution and crystalline framework. The catalytic performance of NiMoO4was investigated for oxidative dehydrogenation of propane. It is demonstrated that the mesoporous NiMoO4catalyst with more surface active oxygen species showed better catalytic performance in oxidative dehydrogenation of propane in comparison with bulk NiMoO4.     

Catalytic properties of Ru nanoparticles embedded on ordered mesoporous carbon with different pore size in Fischer-Tropsch synthesis

A series of 3 wt% Ru embedded on ordered mesoporous carbon (OMC) catalysts with different pore sizes were prepared by autoreduction between ruthenium precursors and carbon sources at 1123 K. Ru nanoparticles were embedded on the carbon walls of OMC. Characterization technologies including power X-ray diffraction (XRD), nitrogen adsorption-desorption, transmission electron microscopy (TEM), and hydrogen temperature-programmed reduction (H₂-TPR) were used to scrutinize the catalysts. The catalyst activity for Fischer-Tropsch synthesis (FTS) was measured in a fixed bed reactor. It was revealed that 3 wt% Ru-OMC catalysts exhibited highly ordered mesoporous structure and large surface area. Compared with the catalysts with smaller pores, the catalysts with larger pores were inclined to form larger Ru particles. These 3 wt% Ru-OMC catalysts with different pore sizes were more stable than 3 wt% Ru/AC catalyst during the FTS reactions because Ru particles were embedded on the carbon walls, suppressing particles aggregation, movement and oxidation. The catalytic activity and C₅₊ selectivity were found to increase with the increasing pore size, however, CH₄ selectivity showed the opposite trend. These changes may be explained in terms of the special environment of the active Ru sites and the diffusion of products in the pores of the catalysts, suggesting that the activity and hydrocarbon selectivity are more dependent on the pore size of OMC than on the Ru particle size.     

Fabrication of ferrocene functionalised ionic liquid/carbon nanotube nanocomposite modified carbon-ceramic electrode: application to the determination of hydrazine

A novel ionic liquid, 1-(ferrocenyl butyl)-3-methylimidazolium tetrafluoroborate (Fc-IL), was synthesised. The nanocomposite of Fc-IL and multi-walled carbon nanotubes (MWCNTs) was constructed and used for surface modification of carbon-ceramic electrode. The modified electrode was applied to the determination of hydrazine. Operational parameters such as pH of the solution, ionic liquid volume and amount of carbon nanotubes, which affect the analytical performance of the modified electrode, were optimised. The linear range of the modified electrode toward hydrazine concentration was 0.96–106.10 μg L^–1 with a detection limit of 0.64 μg L^–1 (S/N = 3). The modified electrode displayed high repeatability, reproducibility, long-term life time and low response time (<3 s). The applicability of this method was further tested by analysing the hydrazine content in boiler-feed water samples containing different concentrations of hydrazine and the results were in good agreement with the spectrophotometry method.     

Self-assembling and size-selective synthesis of Ni and NiO nanoparticles embedded in ordered mesoporous carbon and polymer frameworks

We demonstrate the self-assembling and size-selective synthesis of uniform and highly dispersed Ni or NiO nanoparticles with diameters below 12 nm embedded in ordered mesoporous carbon or polymer frameworks. Self-assembly is induced by evaporation of the solvent from a mixture of metal-containing liquid crystalline (LC) mesophases of triblock copolymer and transition metal nitrate hydrate, and the carbon source is low-polymerized phenolic resol. Characterization by XRD, N(2) sorption isotherms, TEM, HRSEM, ICP-AES, TG, and XPS techniques has indicated an ordered 2D hexagonal mesostructure, high surface areas between 524 and 721 m(2) g(-1), uniform pore sizes of about 4.0 nm, large pore volumes ranging from 0.34 to 0.58 cm(3) g(-1), and metal contents ranging from 0.6 to 10.0 wt%. There is a high degree of dispersion, and a small size of nanoparticles throughout the whole framework, without aggregation outside of the pores due to the confinement effect of the mesoporous ordered matrix. The mesoporous solids show excellent adsorption properties for dyes and permit an easy magnetic separation procedure. This method is expected to be applicable to other mesoporous transition metal(oxide)-containing carbon catalysts.     

高热稳定性有序介孔TiO_2/活性炭制备及其孔-孔协同光催化性能

以十六烷基三甲基溴化铵(CTAB)表面活性剂和钛酸四丁酯分别为造孔模板和钛源,通过超声辅助溶剂挥发自组装技术制备有序介孔氧化钛(ordered mesoporous Ti O2,OMPT)及其活性炭负载体(ordered mesoporous Ti O2/AC,OMPTA).为探讨OMPTA结构与性能之间的关系,采用超声辅助溶胶-凝胶技术合成了无孔氧化钛/活性炭(nonporous Ti O2/AC,NPTA)负载体,利用热重-差热(TG-DTA)、X射线衍射(XRD)、氮气吸附-解吸、透射电子显微镜(TEM)和紫外漫反射(DRS)等手段对制备材料结构进行表征.以酸性红B(acid red B,ARB)的光催化降解为探针实验,评价OMPTA的光催化性能和使用寿命,提出了孔-孔协同光催化扩增机制,并探讨了催化条件(染料浓度、催化剂浓度和溶液p H)对协同扩增效果的影响.结果表明:相对于纯OMPT,OMPTA具有晶粒生长的高活化能、较小的粒径尺寸和对有序介孔结构的高热稳定性,这归功于活性炭的吸附力和非晶相层对晶粒生长的阻碍作用.由于孔-孔协同光催化扩增效应,导致OMPTA在NPTA、OMPT-AC、OMPT、P25和NPT中具有更高的催化活性.热处理温度强烈影响OMPTA的光催化活性,其中OMPTA-500具有最高的光催化活性,这归功于其具备完善的结晶性、相对高浓度的羟基和Ti3+离子.同时,OMPTA-500在重复使用过程中也具有很高的光催化性能.当使用OMPTA-500为催化剂对ARB降解时,最佳的催化条件为催化剂浓度1 g/L,ARB浓度15 mg/L,p H 5.     

N,O共修饰的Ni纳米颗粒/氮掺杂介孔碳CO2高效电还原催化剂

利用可再生电力能源将CO2电还原(CO2RR)为高附加值燃料和化学品(CO、甲酸盐和碳氢化合物等)是一种高效、绿色的CO2资源化利用新技术.然而,由于CO2分子中双键难以活化,且存在析氢竞争反应,即使对于CO2电还原为CO这一简单反应,除少数贵金属(Au、Ag和Pd及其合金)外,当前大多数电催化剂对产物CO的选择性和活性仍较低.因此,开发高效、稳定且廉价的CO2RR催化剂具有重要意义.过渡金属Ni储量高、成本低,是潜在的CO2RR催化剂.然而,受限于Ni对*H及*CO等中间物种相对强的吸附能力,Ni基催化剂催化生成产物CO的活性和选择性较低.近年来研究表明,通过对Ni基材料进行表面修饰,可以调控Ni表面与中间物种的吸附强度,从而有效提升Ni基催化剂对CO2RR反应的活性和选择性.鉴于此,本文通过N,O共调控的策略对负载于N掺杂介孔碳上的Ni纳米颗粒进行表面修饰,制得的N,O-Ni/CMK3催化剂能够高效、高选择性地将CO2电还原为CO.X射线衍射、高角度环形暗场扫描透射电子显微镜和X射线光电子能谱等表征结果表明,N,O-Ni/CMK3中的Ni纳米颗粒由金属Ni核和N掺杂的NiO壳组成,即Ni纳米颗粒表面被N,O共调控,这种独特的表面使其表现出与金属Ni不同的CO2RR催化性能.电化学测试结果表明,在0.5 M KHCO3电解液中,N,O-Ni/CMK3催化剂表现出较好的选择性(生成CO法拉第效率达97％)、活性(CO分电流密度为13.01 mA cm?1)和转换频率(4.25 s?1).表征结果表明,N,O共同调控的Ni是该催化反应的活性中心.此外,得益于N,O共调控的Ni表面,N,O-Ni/CMK3催化剂比O调控的Ni催化剂具有更好的电化学稳定性.本文通过调节Ni催化剂的表面化学环境来调控催化剂与反应中间物种的吸附强度,显著提高了Ni基催化剂对CO2RR反应的催化活性和CO选择性,为开发高活性、高选择性的过渡金属催化剂提供了新思路.     

Synthesis of a new ordered mesoporous NiMo04 complex oxide and its efficient catalytic performance for oxidative dehydrogenation of propane

Highly ordered mesoporous NiMoO4material was successfully synthesized using mesoporous silica KIT-6 as hard template via vacuum nanocasting method. The structure was characterized by means of XRD, TEM, N2adsorption-desorption, Raman and FT-IR. The mesoporous NiMoO4with the coexistence of α-NiMoO4and β-NiMoO4showed well-ordered mesoporous structure, a bimodal pore size distribution and crystalline framework. The catalytic performance of NiMoO4was investigated for oxidative dehydrogenation of propane. It is demonstrated that the mesoporous NiMoO4catalyst with more surface active oxygen species showed better catalytic performance in oxidative dehydrogenation of propane in comparison with bulk NiMoO4.     

Ordered mesoporous carbon film as an effective solid-phase microextraction coating for determination of benzene series from aqueous media

The present work reports preparation of ordered mesoporous carbon (OMC) film supported on a graphite fiber as a new type of solid-phase microextraction (SPME) fiber for determination of benzene series from aqueous media. The strategy for the supported OMC film preparation was combined dip-coating technology with solvent evaporation-induced self-assembly (EISA) approach. A graphite fiber was immersed in an ethanol solution containing phenolic resin and Pluronic triblock copolymer. Upon solvent evaporation and subsequent pyrolysis under 700 °C, the phenolic resin and the surfactant self-assembled on the surface of the graphite fiber to form smooth OMC film. X-ray diffraction (XRD), transmission electron microscopy (TEM) and nitrogen isothermal adsorption results indicate that the resultant OMC film possesses well-ordered two dimensional hexagonal mesostructure with pore diameters of 4.5 nm and BET surfaces of 630 m²/g. Scanning electron microscopy (SEM) studies show the supported OMC film with thickness at 8.5 μm is continuous and defect-free. The SPME efficiency of the OMC fiber was evaluated by analysis of five benzene series (benzene, toluene, ethylbenzene, p-xylene and m-xylene) from water samples by gas chromatography-flame ionization detection (GC-FID). The analysis results indicate that the prepared OMC fiber has wide linear ranges (0.5–500 μg/L), low detection limits (0.01–0.05 μg/L) and good repeatabilities (4.0–5.8% for one fiber, 2.9–8.7% for fiber-to-fiber). Compared with commercial counterparts, the OMC fiber exhibits improved extraction efficiency for benzene series and PAHs.     

Nonenzymatic amperometric sensor of hydrogen peroxide and glucose based on Pt nanoparticles/ordered mesoporous carbon nanocomposite

A simple and facile synthetic method to incorporate Pt nanoparticles inside the mesopores of ordered mesoporous carbons (OMCs) is reported. The Pt/OMCs nanocomposite was characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy, and nitrogen adsorption-desorption. The results show that the incorporation of Pt nanoparticles inside the pores of OMCs does not change the highly ordered two-dimensional hexagonal mesostructure of OMCs matrix. Nonenzymatic amperometric sensor of hydrogen peroxide and glucose based on the Pt/OMCs nanocomposite-modified glassy carbon (GC) electrode is developed. Compared with the original OMCs-modified electrode, the Pt/OMCs-modified electrode displays improved current response towards hydrogen peroxide and gives linear range from 2 to 4212 μM. At an applied potential of −0.08 V, the Pt/OMCs nanocomposite gives linearity in the range of 0.5-4.5 mM glucose in neutral buffered saline solution. This glucose sensor also exhibits good ability of anti-interference to electroactive molecules. The combination the unique properties of Pt nanoparticles and the ordered mesostructure of OMCs matrix guarantees the enhanced response for hydrogen peroxide and glucose.     

Electrochemical behavior of electrodeposited rutin film on a multi-wall carbon nanotubes modified glassy carbon electrode. Improvement of the electrochemical reversibility and its application as a hydrazine sensor

By immobilizing rutin at the surface of a glassy carbon electrode (GCE) modified with multi-wall carbon nanotubes (MWCNT), a new modified electrode has been fabricated and its electrochemical behavior was investigated by cyclic voltammetry. Cyclic voltammograms of the resulting modified electrode show stable and a well defined redox couple with surface confined characteristics. The results show that the reversibility of rutin is significantly improved at a MWCNT modified GCE in comparison with GCE alone. The charge transfer coefficient, α, was calculated to be 0.4, and charge transfer rate constant, k_s, was 46.7 s⁻¹ in pH 8, indicating great facilitation of the electron transfer between rutin and MWCNT deposited on the electrode surface. The rutin MWCNT (RMWCNT) modified GCE showed excellent mediation of hydrazine oxidation: a decrease in the overvoltage of hydrazine electrooxidation was observed as well as a dramatic increase in the peak current compared to that seen at a rutin modified GCE (RMGCE), activated GCE or bare GCE. Hydrazine was determined amperometrically at the surface of RMWCNT modified GCE in pH 8. Under the optimized conditions the calibration curve is linear in the concentration range 2.0–190.0 μM hydrazine. The detection limit and sensitivity are 0.61 μM and 0.0656 μA μM⁻¹, respectively. Finally the kinetic parameters of the electron transfer coefficient, α, the heterogeneous rate constant of dependent to different potentials, k′(E), and the standard heterogeneous rate constant, k⁰, for oxidation of hydrazine at the RMWCNT surface were determined using various electrochemical methods. The advantages of this modified electrode for hydrazine determination are high sensitivity, excellent catalytic activity, short response time, wide linear range, and high exchange current density.     

基于介孔碳的电化学酪氨酸酶生物传感器法测定水体中的苯酚及高效液相色谱法评价

采用新型的介孔碳材料作为固载酪氨酸酶的检测平台构建生物传感器,应用于水体环境中苯酚污染物的检测,并通过高效液相色谱法对电化学酪氨酸酶生物传感器法的准确性进行了评价。研究表明,介孔碳的"空间限制效应"能够防止酪氨酸酶(三维尺寸为6.5 nm×9.8 nm×5.5 nm)体外去折叠失活。基于介孔碳材料构建的电化学酪氨酸酶生物传感器在苯酚污染物检测方面显示了优良的性能,其重现性、灵敏度、稳定性、选择性以及检出限均比较令人满意。基于介孔碳的电化学酪氨酸酶生物传感器对苯酚污染物的检出限达到20 nmol/L,线性范围为0.1~10 μmol/L。采用基于介孔碳的电化学酪氨酸酶生物传感器和高效液相色谱法对实际水样品进行测定结果比对,结果表明该生物传感器方法检测结果准确、有效,适合于苯酚污染物突发污染事件的应急检测。