米托蒽醌在离子注入修饰电极上的伏安行为及其应用

在0.1mol/LNH₃-NH₄Cl缓冲溶液(pH_9.5)中,米托蒽醌在镍离子注入修饰电极上有一灵敏的伏安还原峰,峰电位为-0.85V(vs.SCE).峰电流与米托蒽醌的浓度成线性关系.检出限为1.8×10^-8mol/L.研究了其伏安行为,并将该波用于尿样的测定.结果表明,还原过程为具有吸附性和催化性的准可逆过程.AES和XPS实验表明,Ni已注入到玻碳电极的表面,使电催化活性提高.     

柔红霉素在钴离子注入修饰玻碳电极上与DNA相互作用

用钴离子注入修饰电极研究了柔红霉素与DNA的相互作用.柔红霉素以嵌入方式与DNA发生作用,形成非电活性的结合物.加入DNA后,柔红霉素的电化学行为没有改变,仍为扩散控制.用非线性拟合得到柔红霉素与DNA的结合常数K=1.09×10⁸cm³/mol,结合数s≈4.DNA分子结构中的1个螺旋结合2个柔红霉素.     

阿霉素在钴离子注入修饰电极上的电化学行为及其应用

阿霉素在０．１ｍｏｌ／Ｌ ＨＡｃ－ＮａＡｃ（ｐＨ４．６２）缓冲溶液中用注入钴的修饰玻碳电极为工作电极进行伏安测定,得到一良好的还原峰;Ｅｐ＝－０．５２２Ｖ（ｖｓ．ＳＣＥ）。峰电流与阿霉素浓度在１．４×１０＾－６－１．４×１０＾－６ｍｏｌ／Ｌ和１．４×１０＾－６－５．６×１０＾－５ｍｏｌ／Ｌ范围内呈线性关系,。     

马兜铃酸在钴离子注入修饰碳纤维电极上的电化学行为及其应用

马兜铃酸在 0 0 8mol LNa2 HPO4 KH2 PO4 溶液 (pH 6 82 )中 ,于钴离子注入修饰碳纤维电极 (Co CFUE)上 ,产生一个具有电催化性质的还原波。还原波峰电位为 - 0 80V(vs.SCE)。在此电极上 ,马兜铃酸的电化学反应过程是准可逆的。用扫描电子显微镜 (SEM)和俄歇电子能谱 (AES)两种表面技术对钴离子注入碳纤维和普通碳纤维表面进行表征 ,证明由于Co离子的注入 ,改变了碳纤维基体的性质 ,催化了马兜铃酸的还原。该波的一阶导数峰电流与马兜铃酸的浓度在 1 6× 1 0 -7～ 1 4× 1 0 -4 mol L范围内呈线性关系 ,检出限为 7 6× 1 0 -8mol L。用于马兜铃生药的测定 ,回收率在 97 5 %～ 1 0 3 3%之间。     

马兜铃酸在钴离子注入修饰电极上的电化学行为及其应用

马兜铃酸在 0 .2mol/LHAc -NaAc缓冲溶液 (pH 4.6)中 ,用钴离子注入修饰电极为工作电极进行伏安测定 ,得到一良好的还原峰 ,峰电位为 -0 .71V(vs .SCE) ,峰电流与马兜铃酸的浓度在 1.2× 10 - 7～ 3 .7×10 - 5mol/L之间呈线性关系 ;检出限为 8.6× 10 - 8mol/L。用于人工合成试样的测定 ,结果满意。用线性扫描、循环伏安法研究了体系的性质。结果表明 :马兜铃酸的电极过程是具有吸附性的不可逆过程。用AES和XPS表面分析技术对注入电极表面和未注的玻碳电极表面进行了表征 ,探讨了离子注入电极的催化性质     

氨甲蝶呤在Co/GC离子注入修饰电极上电化学行为及其应用研究

氨甲蝶呤在0.1mol/LHAc-NaAc(pH=4.96)缓冲溶液中,用Co/GC离子注入修饰电极进行伏安测定,得到一良好的还原峰,峰电位为-0.95V(vs.SCE).峰电流Ip与氨甲蝶呤的浓度在2.2×10^-7～8.8×10^-6mol/L范围内呈线性关系。检出限为1.1×10^-8mol/L.建立了测定氨甲蝶呤的新方法,可用于实际样品的测定,回收率在98.9%～106.2%之间。用线性扫描和循环伏安法研究了体系的电化学行为及电极反应机理。实验表明,氨甲蝶呤的还原为不可逆吸附过程,并伴随两个电子和两个氢离子参与电极反应。用俄歇电子能谱(AES)和X射线光电子能谱(XPS)等表面分析技术对Co离子注入修饰电极的表面元素组成及深度分布等进行测定,表明Co离子确被注入到玻碳表面。扫速对催化效率的影响实验证明体系存在催化作用。     

米托蒽醌在金电极上的电化学研究及其应用

米托蒽醌(MTX)在0.2 mol/L B-R缓冲溶液(pH=1.5)中循环伏安扫描时,在金电极上会产生一个灵敏的氧化峰P1(峰电位为1.087 V)和两个灵敏的还原峰P2(峰电位为0.817 V)、P3(峰电位为0.764 V)。P1峰电流值与MTX浓度在1.0×10-9~1.0×10-6mol/L范围内呈良好的线性关系;其检出限可达5.6×10-10mol/L。本研究优化了测定米托蒽醌的最佳实验条件,建立了可灵敏测定米托蒽醌的新方法;同时对米托蒽醌在金电极上的电化学行为进行了较为详细的研究。     

辅酶I在钴/玻碳离子注入修饰电极上的电化学行为及其应用研究

辅酶I(NAD)在 0 .0 0 5mol/LTris 0 .0 1mol/LNaCl(pH =7.0 )缓冲溶液中 ,在Co/GC离子注入电极上有一良好的伏安还原峰 ,峰电位Ep=- 1 .0 7V (vs .SCE)。其峰电流与辅酶I (NAD)的浓度在 5 .0× 1 0 -5～ 1 .0×l0 -3 mol/L范围内呈线性关系 ;检出限为 2 .5× 1 0 -5mol/L ;回收率在 98.8%～ 1 0 1 .9%之间。用线性扫描和循环伏安法等方法研究该体系的电化学行为 ,实验表明 :该体系具有吸附性和催化性的不可逆过程。根据Laviron理论 ,求得电极表面反应速率常数Ks=0 .0 77/s;电子转移系数α =0 .4 35。     

Electrochemical Studies of Pirarubicin and Its Interaction with DNA at a Co／GC Ion Implantation Modified Electrode

IntroductionIon implantation is a new material surfacemodification technique.It has been also applied tostudying the electrochemical behaviors of organicdrugs and biological materials as well as their de-terminations. This method offers good stability,reproductivity and catalytic activity[1] .Pirarubicin( THP) is an active highly effective and new antitu-moral anthracycline antibiotic,which has gainedwidespread clinical use in the chemotherapeutictreatment of a variety of human cancers.The d…     

Electrochemical Behavior of Adriamycin at Ni/GC Ion Implantation Modified Electrode

Ionimplantationisakindofnewmaterialsurfacemodificationtechnique.ltsapplicationoffersthefollowingn1arkedadvantagest(l)ItcanimpIantdifferentionsintodifferentmaterialsurfacesaccordingtodifferentdesiresandrequirementstomakemodifiedelectrodeswithcatalyticactivity;(2)TheimpIantedsurfacecausesdefectandpartialdislocationtoformmanyactivecenters,itscatalyticactivityismuchhigherthanthatoftherawmaterial;(3)TheionimplantationmodifiedelectrodehasgoodstabilityandreproducibiIity.Adriamycin(ADM)isthemostpro…     

Electrochemical Behavior of Mitoxantrone and Its Determination at a Pt／C Implanted Modified Microelectrode

In a 0.02 mol/L Na2HPO4-KH2PO4(PBS) buffer solution(pH=6.82), the electrochemical behavior of mitoxantrone was studied by linear-sweep voltammetry and cyclic voltammetry at a Pt/C ion implantation modified microelectrode. A sensitive reduction peak was observed. The peak potential was -0.72 V(vs.SCE), the peak current was proportional to the concentration of mitoxantrone within the ranges of 7.0×10-8-9.0×10-7 mol/L and 1.0×10-6-2.4×10-5 mol/L, with a detection limit of 4.0×10-8 mol/L. The linear correlation coefficients were 0.9994 and 0.9992, respectively. This method has been applied to the direct determination of mitoxantrone in simulated urine. The recoveries were in the range from 96.2% to 105.9%. The reduction process was a quasi-reversible one with adsorptive characteristics at the Pt/C microelectrode. The electrode reaction rate constant ks and the electron transfer coefficient α of the system were determined to be 4.5 and 0.65 s-1, respectively. The experiments showed that Pt element had surely been implanted into the surface of the carbon fiber, and the atomic Pt improved the electrocatalytic activity. The Pt/C microelectrode had a good stability and reproducibility.     

Studies on Electrochemical Behavior of Bleomycin and Its Interaction with DNA at a Co／GC Ion Implantation Modified Electrode

ＩｎｔｒｏｄｕｃｔｉｏｎＩｏｎｉｍｐｌａｎｔａｔｉｏｎｉｓａｋｉｎｄｏｆｎｅｗｍａｔｅｒｉａｌｓｕｒｆａｃｅｍｏｄｉｆｉｃａｔｉｏｎｔｅｃｈｎｉｑｕｅ .Ｒｅｃｅｎｔｌｙ ,Ｌｉｅｔａｌ .1ｈａｖｅｌｅｄｔｈｉｓｔｅｃｈｎｉｑｕｅｉｎｔｏａｎａｌｙｔｉｃａｌｃｈｅｍｉｓｔｒｙａｎｄｍａｄｅｍｏｄｉ ｆｉｅｄｅｌｅｃｔｒｏｄｅｗｉｔｈｐａｒｔｉｃｕｌａｒｆｕｎｃｔｉｏｎ .Ｉｔｈａｓｂｅｅｎａｐ ｐｌｉｅｄｔｏｓｔｕｄｙｔｈｅｅｌｅｃｔｒｏｃｈｅｍｉｃａｌｂｅｈａｖｉｏｒｓａｎｄｔｈｅｄｅ ｔｅｒｍｉｎａｔ…     

Studies on the Electrochemical Behavior of Bleomycin at a Co/GC Ion Implantation Modified Electrode and Its Application

In a 0. 10 mol/L HAc-NaAc buffer solution (pH = 4. 59), a sensitive reduction peak of bleomycin was observed by linear sweep voltammetry at a Co/GC ion implantation modified electrode. The peak potential was-0. 73 V(iw. SCE). The peak current was proportional to the concentration of bleomycin over the range of 5.0 × 10-8-1.0× 10-6 and 1.0× 10-6-1. 0 × 10-5 mol/L with a detection limit of 2.0 × 10-8 mol/L. The electrochemical behavior of the reduction peak of bleomycin at the Co/GC modified electrode was studied by linear sweep and cyclic voltammetry and applied to the determination of bleomycin in urine. This method is simple, rapid and reliable. The reduction process is quasi-reversible. The experiments of AES and XPS showed that Co was surely implanted into the surface of GCE and the depth distribution of Co was in good agreement with Gooses normal distribution; the implanted Co at GCE improved the electrocatalytic activity.     

Electrochemical Studies of Adriamycin Interaction with Dna and Determination of Dna at a Ni/Gc Ion Implantation Modified Electrode

ABSTRACT

The electrochemical behavior of the reduction of adriamycin at Ni/GC modified electrode has been studied by linear sweep and cyclic voltammetry. The reaction of DNA with ADM formed an electrochemically nonactive complex, which resulted in a decrease in the ADM equilibrium concentration and its reduction current. The decrease in peak current was proportional to DNA concentration and can be used to determination DNA concentration. The experiments of AES and XPS showed that Ni is surely implanted into the surface of GCE and the depth distribution of Ni was in good agreement with Gooses normal distribution; the implanted Ni at GCE improved the electrocatalytic activity.     

吡柔比星在钴离子注入修饰微电极上电化学行为及其应用

吡柔比星在0.1mol/LHAc-NaAc(pH=5.05)缓冲溶液中,用Co离子注入修饰碳纤维微电极进行研究,得到一良好的还原峰,峰电位Ep=-0.520V(vs.SCE).峰电流与吡柔比星浓度在1.0×10^-7～6.0×10^-6mol/L范围内成线性关系,检出限为5.0×10^-8mol/L.用于病人尿样测定,回收率在91.6%～106.7%之间.吡柔比星的还原为可逆吸附过程,伴随着两个电子、两个氢离子参加电极反应.用扫描电子显微镜(SEM)和俄歇电子能谱(AES)两种表面分析技术对Co离子注入修饰微电极的表面状况、表面元素组成及深度分布进行测定.实验表明,Co离子确实被注入到碳纤维表面上.扫速对催化效率的影响实验证明体系存在催化作用.