汞灯光源芯片毛细管电泳荧光电荷耦合器件检测系统

以宽谱带高压汞灯为光源的倒置荧光显微镜,配备电荷耦合器件(CCD)及微流控芯片,自组装芯片毛细管电泳荧光电荷耦合器件检测系统。选择不同激发波长(340~540 nm)荧光试剂,进行荧光检测,荧光素的检出限(S/N=3)为7.3×10-9mol/L,荧光素异硫氰酸酯(FITC)的检出限(S/N=3)为1.7×10-8mol/L,显示了该系统对荧光试剂选择范围宽、灵敏度高的特点。用该系统成功分离了FITC与荧光素、曙红与荧光素两种混合荧光试剂。实验表明,组装的芯片毛细管电泳CCD荧光检测系统是成功的。     

小型可连续进样微流控芯片分析的研制

报道了一种结构简单、可连续进样的小型控芯片分析仪的研制.顺序注射分析系统通过芯片上制作的接口将试样连续引入芯片,并采用自行设计的紧凑型光纤式激光诱导荧光检测器进行检测.该仪器用于芯片毛细管电泳分离实验室合成Cy5荧光染料,实现了连续进样和换样.峰高RSD为1.9%(n=11),试样通量35/h;相邻试样携出＜4%.     

一种连接石英毛细管的聚甲基丙烯酸甲酯电泳芯片的制作及其应用

利用模拟仿真软件模拟了死体积对于电泳分离的影响,并提出了一种实现聚甲基丙烯酸甲酯(Poly-methylmethacrylate,PMMA)电泳芯片与毛细管的最小死体积连接方法.将紫外吸收检测的检测窗口设计在石英毛细管上,使该PMMA芯片上的电泳分析可以直接采用紫外吸收检测器进行检测.利用该芯片对维生素B2进行了电泳分析,理论塔板数为73000/m;联磺甲氧苄啶片中的3种组分的分离度为4.5和1.9,3个峰迁移时间的RSD依次为1.0%,1.4%和1.0%,峰高的RSD依次为4.1%,3.0%和4.1%(n=5).     

顺序注射-液芯波导H-通道芯片联用系统DNA片段的毛细管电泳分离激光诱导荧光检测

毛细管电泳微流控芯片分离-激光诱导荧光(LIF)检测DNA片段是近年来微流控分析系统中研究得较为成功的领域,该方向的研究成果极大地促进了微流控分析系统的发展.在相关的报道中,待分析样品和系统运行溶液仍然主要使用手工操作.     

激光诱导荧光检测微流控芯片生化分析仪的研制

研制出一种集激光诱导荧光检测、微流控芯片电泳及控制系统于一体的生化分析仪。分析仪内采用可重复使用的玻璃基质微流控芯片,利用销式固定技术实现芯片的精确定位,定位精度达到±2μm。以四触点高电压系统控制芯片上的进样和电泳分离操作。激光诱导荧光检测系统采用正交光路模式,对Cy5染料的检出限达到1.0×10-10mol/L(S/N=3)。以羟乙基纤维素为筛分介质,初步进行了ΦΧ174-HaeШdigest DNAmarker限制性片段的毛细管电泳分离。     

毛细管电泳与芯片毛细管电泳的双检测技术

综述了毛细管电泳和芯片毛细管电泳的3种双检测技术,包括荧光-散射等光学双检测技术、安培-非接触电导等电化学双检测技术和荧光-非接触电导等光电联用双检测技术.介绍了3种双检测方法的仪器的检测原理及应用,并展望了双检测技术的发展前景.引用文献54篇.     

带有可更换半自准工作电极的集成化毛细管电泳安培检测芯片(Ⅱ)——实验参数对多巴胺半波电位的影响

采用带有可更换半自准式集束碳纤维圆盘工作电极的集成化毛细管电泳安培检测芯片,以多巴胺为模型化合物,通过考察分离电压、通道构型及电极与通道出口的间隙等参数对待测物多巴胺半波电位的影响,提出了芯片毛细管电泳-柱端安培检测系统中减小分离电压对安培检测系统干扰的措施.     

单细胞分析的研究

单细胞分析是分析化学、生物学和医学之间渗透发展形成的跨学科前沿领域。近年来,毛细管电泳及微流控芯片用于单细胞分析已取得显著进展,特别表现在微流控芯片用于细胞的培养、分选、操纵、定位、分离及检测细胞的组分,实时监测细胞释放,及高通量阵列检测等方面。芯片的单元操作可根据需要灵活组合,显示出其独特的优点。本文重点介绍作者研究组的工作,并对近三年来国内外在毛细管电泳及芯片毛细管电泳用于单细胞分析的新进展进行评论。最后从毛细管电泳与微流控芯片、微流控芯片与细胞界面以及量子点用于探测活细胞等方面,展望了单细胞分析的发展前景。     

微流控芯片上的细胞分析研究进展

近年来,微流控分析系统(μTAS)在生物细胞分离领域的发展引起了广泛的关注。微流控芯片的微米级尺寸的通道适合于单细胞样品的引入、操控、反应、分离和检测,已经在微芯片上实现了上述功能,并将这些功能集成在具备毛细管电泳分离功能的微芯片上。     

微流控芯片毛细管电泳在蛋白质分离分析中的应用研究进展

重点介绍了近年来国内外在微流控芯片毛细管电泳法用于蛋白质分离分析方面的研究进展。按照分离模式的不同,综述了各种应用于蛋白质分离的微流控芯片毛细管电泳系统,讨论了抑制芯片中的蛋白吸附的各种方法,并展望了芯片毛细管电泳系统在蛋白质分离领域的发展前景。引用文献47篇。     

微流控芯片流动注射气体扩散分离光度测定系统的研究

提出了纳升级进样量的微流控芯片流动注射气体扩散分离光度检测系统. 制作三层结构微流控芯片, 在玻璃片上加工微反应通道, 用聚二甲基硅氧烷[Poly(dimethylsiloxane), PDMS]加工气体渗透膜和具有接收气体微通道的底片, 实现了生成气体的化学反应、气-液分离和检测在同一微芯片上的集成化. 采用缝管阵列纳升流动注射进样系统连续进样, 用吸光度法测定NH+4以验证系统性能. 结果表明， 该系统对NH+4的检出限为140 μmol/L(3σ), 峰高精度为3.7%(n=9). 在进样时间12 s、注入载流48 s和每次进样消耗200 nL试样条件下, 系统分析通量可达60样/h. 若加大样品量到800 nL, 使接收溶液停流1 min, 该系统对NH+4的检出限可达到35 μmol/L(3σ), 但分析通量降低到20样/h.     

带有可更换半自准工作电极的集成化毛细管电泳安培检测芯片

自Woolley等首次报道集成于玻璃芯片上的微型毛细管电泳-安培检测(Chip-based capillary electrophoresis with amperometric detection,CE-AD)系统以来,CE-AD以其高效、高速、高灵敏度以及易微型化集成化等特点引起研究者的关注．在芯片上实现柱端安培检测可用直接制作在芯片上的喷(镀)膜工作电极,或采用外置的壁喷式电极。前者集成化程度高,后者的工作电极可以更换,大大提高了芯片的重复利用率。     

集成毛细管电泳芯片系统的制作、测试及应用

使用标准光刻和化学湿法腐蚀技术,在玻璃板材上制作了由样样管道和分离管道内构成的集成毛细管网路系统,对影响芯片质量的一些因素进行了讨论,并进行了性能测试和评价。芯片上毛细管道散热良好。使用激光诱导荧光和CCD成像检测系统,以电渗作用为驱动力,对混合样品进行了进样、快速分离（20s以内）和监测,证明了自制集成毛细管电泳芯片及检测系统的可行性。比较了两种注样方式（float和pinched)的不同;证明了在分离时可以优化加电策略,防止拖尾,改善峰形。     

集成化液膜萃取-反萃取-毛细管电泳联用芯片

我们设计并制作了集成有支持液膜萃取-反萃取试样预处理的毛细管电泳(SLMEBE-CCE)微流控芯片. 分别以荧光素钠和丁基罗丹明B作为模型待测物和共存物, 在该芯片上进行了在线试样预处理与毛细管电泳联用的初步实验.     

Electrochemical Detection Using an Engraved Microchip – Capillary Electrophoresis Platform

The present study describes a simple strategy to integrate electrochemical detection with an assembled microchip‐capillary electrophoresis platform. The electrochemical cell was integrated with a microfluidic device consisting of five plastic squares interconnected with fused silica capillaries, forming a four‐way injection cross between the separation channel and three side‐arms (each of 15 mm in length) acting as buffer/sample reservoirs. The performance of the system was evaluated using electrodes made with either carbon ink, carbon nanotubes, or gold and under different experimental conditions of pH, capillary length, and injection time. Using this system it was possible to separate the neurotransmitters dopamine and cathecol and to quantify phenol from a real sample using a linear calibration curve with a calculated LOD of 0.7 µM. A similar concept was applied to determine glucose, by including a pre‐reactor filled with beads modified with glucose oxidase (GOx). The latter system was used to determine glucose in a commercial sample, with a recovery of 95.2 %. Overall, the presented approach represents a simple, inexpensive, and versatile approach to integrate electrochemical detection with CE separations without requiring access to microfabrication facilities.     

A capillary electrophoresis chip with hydrodynamic sample injection for measurements from a continuous sample flow

A microchip-based capillary electrophoresis device supported by a microfluidic network made of poly(dimethylsiloxane), used for measuring target analytes from a continuous sample flow, is presented. The microsystem was fabricated by means of replica molding in combination with standard microfabrication technologies, resulting in microfluidic components and an electrochemical detector. A new hydrodynamic sample injection procedure is introduced, and the maximum number of consecutive measurements that can be made with a poly(dimethylsiloxane) capillary electrophoresis chip with amperometric detection is investigated with respect to reproducibility. The device features a high degree of functional integration, so the benefits associated with miniaturized analysis systems apply to it.     

Small-angle optical deflection from collinear configuration for sensitive detection in microfluidic systems

This paper describes a novel detection system based on small-angle optical deflection from the collinear configuration of a microfluidic chip. In this system, the incident light beam was focused on the microchannel through the edge of a lens, resulting in a small deflection angle that deviated 20° from the collinear configuration. The emitted fluorescence was collected through the center of the same lens and delivered to a photomultiplier tube in the vertical direction; the reflection light of the chip plate was kept away from the detector. In contrast to traditional confocal and nonconfocal laser-induced fluorescence detection systems, background levels resulting from scattered excitation light, reflection and refraction from the microchip was significantly eliminated. Significant enhancement of the signal-to-noise ratio was obtained by shaping a laser beam that combined an attenuator with a spectral filter to optimize laser power and the dimensions of the laser beam. FITC and FITC-labeled amino acid were used as model analytes to demonstrate the performance sensitivity, separation efficiency, and reproducibility of this detection system by using a hybrid polydimethylsiloxane/glass microfluidic device. The limit of detection of FITC was estimated to be 2 pM (0.55 zmol) (S/N = 3). Furthermore, the single cell analysis for the determination of intracellular glutathione in a single 3T3 mouse fibroblast cell was demonstrated. The results suggest that the proposed optical arrangements will be promising for development of sensitive, low-cost microfluidic systems.     

Parallel analysis of biomolecules on a microfabricated capillary array chip

This paper focused on a self-developed microfluidic array system with microfabricated capillary array electrophoresis (mu-CAE) chip for parallel chip electrophoresis of biomolecules. The microfluidic array layout consists of two common reservoirs coupled to four separation channels connected to sample injection channel on the soda-lime glass substrate. The excitation scheme for distributing a 20 mW laser beam to separation channels in an array is achieved. Under the control of program, the sample injection and separation in multichannel can be achieved through six high-voltage modules' output. A CCD camera was used to monitor electrophoretic separations simultaneously in four channels with LIF detection, and the electropherograms can be plotted directly without reconstruction by additional software. Parallel multichannel electrophoresis of series biomolecules including amino acids, proteins, and nucleic acids was performed on this system and the results showed fine reproducibility.     

芯片毛细管电泳中组分的迁移行为及其特征

在自组装的芯片毛细管电泳-激光诱导荧光检测装置上,以单个染料和一组荧光素异硫氰酸酯(FITC)标记的氨基酸为对象,研究了芯片毛细管电泳与传统毛细管电泳之间的差别,考察了玻璃芯片上微通道内的伏安特性以及抑制电压、进样方式和检测点的位置等对芯片毛细管电泳分离分析的影响,特别注意到了其有别于传统毛细管电泳的各种行为特征.     

超高速平板通道毛细管电泳

超高速平板通道毛细管电泳是９０年代发展的一种秒级分离的新颖技术。应用现代微电子光刻技术将化学反应。进样、分离和检测等组合在数厘米玻片上。实现分离分析的小型化、集成化、一体化和自动化。