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核酸适配体(简称适配体)是一类重要的"化学抗体"型功能性生物分子,基于适配体的质谱技术在提供分子质量及结构特征等基础上,兼具了针对靶分子的高选择性及亲和富集特点,从而提供出高特异、高灵敏信息。本文综述了近年来适配体在质谱分析中的应用进展,重点评述了适配体在质谱分子相互作用表征中的应用、适配体作为离线型和在线亲和材料用于质谱分析测定等现状,其中结合多种质谱新技术,通过创建或结合多种前处理或在线一体化信号增强方式,特别是适配体功能化纳米材料的应用,在相互作用表征、痕量靶分子选择性提取和高灵敏检测等质谱分析测定方面是研究的重点。最后,本文对适配体在质谱研究中的应用前景进行了展望。 相似文献
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目前,临床上使用的许多抗病毒药物均是通过与DNA,RNA发生相互作用破坏其结构,进而影响基因调控与表达的功能,表现出抗病毒活性。因此,核酸与药物分子相互作用的研究对阐述抗病毒药物的作用机理,以及对药物的体外筛选都具有重要的意义.电喷雾电离质谱作为一种软电离手段,可将溶液中生物分子与药物分子的非共价复合物转为气相进行分析,再现其生理状态,使其成为分子水平上进行药物筛选的最佳方法和在分子水平上筛选中药抗病毒活性成分的理想工具,本文选择合成了与SARS病毒相关的DNA片段作为抗病毒药物筛选的靶分子,用电喷雾质谱技术,通过对靶分子与5种生物碱的非共价复合作用,探讨了生物质谱方法用于药物筛选的可行性。 相似文献
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建立了检测农药小分子与生物大分子(BSA蛋白,SOD酶)相互作用的高分辨质谱分析方法.对相应结合产物进行质谱检测,结果表明,BSA蛋白与甲基对硫磷分子在开始相互作用30 min后达到饱和,且每个蛋白分子最多与5个甲基对硫磷分子结合;BSA蛋白与甲维盐不存在相互作用.通过对SOD酶与敌敌畏,阿维菌素及噻呋酰胺结合产物的质谱检测发现每个SOD酶最多结合2个敌敌畏分子,1个阿维菌素分子,且不与噻呋酰胺相互作用.此外,实验表明,SOD酶与阿维菌素分子作用30 min后达到平衡,与敌敌畏分子作用20 min后达到平衡.通过对两种蛋白结合农药小分子过程的时间分辨分析发现,两种生物大分子结合农药小分子的机理过程存在差异.本方法与相关标准方法(荧光光谱法,NBT试剂盒法)及分子模拟对接结果比对说明,本方法切实可靠.本方法具有耗样量少、检测速度快、可提供多方面信息等优点,在新农药的研发及其安全性评价方面具有实用价值. 相似文献
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用合适的手段表征生物分子的相互作用对于深刻理解生命过程的本质以及进行医药开发都具有重要意义。将微流控芯片和毛细管电泳相结合的微流控芯片电泳技术具有快速、高效、高通量、样品用量少和易于整合等诸多优势。本文对近年来进行生物分子间相互作用结合常数测定以及结合动力学研究的微流控芯片电泳分离模式、分析方法和芯片检测方法分别做了介绍;简单对比了微流控芯片技术和微阵列生物芯片生物分子间相互作用研究技术;最后分析了微流控芯片技术目前的不足,并对其未来的发展进行了展望。 相似文献
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蛋白质组学已经成为生命科学研究中最为活跃的领域之一。研究蛋白质的生物功能,不但需要高通量的鉴定蛋白质,还需要定量分析动态变化的蛋白质,即定量蛋白质组学研究。蛋白质的定量研究有助于发现新的生物功能,并可以用于疾病的预警和药物靶点的发现。现有的定量蛋白质组学研究主要利用同位素标记结合生物质谱(电喷雾电离质谱ESI-MS,基质辅助激光解吸电离质谱MALDI-MS)技术而实现。近年来电感耦合等离子体质谱(ICP-MS)作为ESI-MS和MALDI-MS的补充,越来越多地应用于蛋白质的定量分析,特别是蛋白质的绝对定量分析。ICP-MS是检测生物分子中痕量元素的理想工具,具有灵敏度高、动态范围广,不易受基体的影响等优点。本文将讨论基于ICP-MS的分析方法,及其在蛋白质定量分析和免疫分析中的部分成功应用。 相似文献
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荧光素与牛血清蛋白的作用及其分析应用 总被引:2,自引:0,他引:2
1引言根据染料与蛋白质的相互作用建立测定蛋白质的分析方法已有不少报道,但寻找简便、快速、准确的测定蛋白质的分析方法仍然是人们研究的热点。生物大分子与小分子配体相互作用的研究,目前多用透析,超滤或凝胶色谱等实验方法。当小分子配体与生物大分子结合前后的吸收光谱有一定差别时,用光度法在不经分离的情况下进行研究比上述方法要简便得多,且实验得信息较为可靠。本文正是利用荧光素与BSA相互作用形成复合物最大吸收峰的波长480nm,比试剂本身红移约9nm,提出的分析方法,线性范围宽,灵敏度高,方法简便、快速,干… 相似文献
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用溶胶-凝胶法以磷钼酸(MPA)的镍盐溶液水解钛酸四丁酯制备了NiPMo/TiO2催化剂.使用ICP、 XRD、 TG-DTA、 IR、 TPD-MS和微反应技术研究了催化剂的化学组成、热稳定性、化学吸附性质和催化反应性能.杂多钼酸盐与TiO2通过O2-在TiO2表面发生了键合.在623 K下,杂多阴离子仍保持原有的Keggin结构.CO2在Lewis酸位Ni(Ⅱ)和Lewis碱位Ni-O-Mo的桥氧协同作用下生成CO2卧式吸附态Ni(Ⅱ)←O-(CO)←(O--Ni).丙烯有多种吸附态在催化剂上吸附.在563 K、 1 MPa和空速1500 h-1的反应条件下,丙烯的摩尔转化率为3.2%,产物MAA选择性为95%. 相似文献
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Toward new camptothecins. Part 6: Synthesis of crucial ketones and their use in Friedländer reaction
Laurent Gavara Thomas Boisse Jean-Pierre Hénichart Adam Daïch Philippe Gautret 《Tetrahedron》2010,66(38):7544-5571
In the context of the preparation of camptothecin and luotonin A analogs, the synthesis of some key keto-precursors and their use in Friedländer condensation are described. This paper also focuses on the stability of these keto intermediates and emphasizes the major differences between indolizinones and pyrroloquinazolinones series. Noteworthy is also the report of some original structures isolated as by-products of some experiments. 相似文献
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The Langevin paramagnetic theory can’t describe the relation between magnetization of ferrofluids and applied magnetic field. The structuralization of ferrofluids, which is considered the main influence factor of the magnetization, is regarded. The part of magnetization works is deposited when the structure is forming. This action influences the magnetization of ferrofluids directly or indirectly. On the base of the “compressing” model, the Langevin function that usually describes the magnetization of ferrofluid is modified, and a well-fitted curve is obtained. An equation of the relation between the equivalent volume fraction after being “compressed” and the intensity of magnetic field is discovered, which approximately describes the process of magnetization. The relation between the approximate initial susceptibility and the volume fraction can be obtained from modified formula. 相似文献
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Rebecca J. Burton Mandy L. CrowtherNeal J. Fazakerley Shaun M. FilleryBarry M. Hayter Jason G. KettleCaroline A. McMillan Paula PerkinsPeter Robins Peter M. SmithEmma J. Williams Gail L. Wrigley 《Tetrahedron letters》2013
The highly regioselective Buchwald–Hartwig amination at C-2 of the cheap and readily accessible reagent, 2,4-dichloropyridine with a range of anilines and heterocyclic amines is described. This new methodology is robust and provides a facile access to 4-chloro-N-phenylpyridin-2-amines on 0.25 mol scale. These intermediates undergo a further Buchwald–Hartwig amination at higher temperature to enable rapid exploration of the chemical space at C-4 and to provide a library of 2,4-bisaminopyridines. 相似文献
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KMnO4-mediated oxidative CN bond cleavage of tertiary amines producing secondary amine was introduced, which was trapped by electrophiles (acyl chloride and sulfonyl chloride) to form amides and sulfonamides. The reaction could take place at mild condition, tolerating a wide range of function groups and affording products in moderate to excellent yields. 相似文献
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N. A. Nedolya 《Chemistry of Heterocyclic Compounds》2008,44(10):1165-1219
The review contains a concise historical account and information on the most significant researches undertaken by the staff
at the A. E. Favorsky Irkutsk Institute of Chemistry, Siberian Branch of the Russian Academy of Sciences on the Chemistry
of Heterocyclic Compounds.
Dedicated to Academician of the Russian Academy of Sciences B. A. Trofimov on his 70th jubilee.
Translated from Khimiya Geterotsiklicheskikh Soedinenii, No. 10, pp. 1443–1502, October, 2008. 相似文献
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Regiospecific and direct imidation of the methyl C(sp3)–H bond of thioanisoles is realized under mild and metal-free conditions with N-fluorobis(benzenesulfonyl)imide as an oxidant and nitrogen source. Proposed mechanism suggests that thionium ion intermediates and a Pummerer-type reaction are involved. The imidation has advantages such as high step-economy, excellent functionality tolerance, and regiospecificity, giving structurally diverse imidation products. 相似文献
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Different approaches for the synthesis of 1-benzyloxypyrazin-2(1H)-one derivatives from simple amino acids have been investigated. A library of 33 precursors for the preparation of N-hydroxy pyrazinones was obtained in moderate to good yields. 相似文献
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《Tetrahedron》2014,70(21):3377-3384
The Rh(II)-catalyzed reaction of 2-carbonyl-substituted 2H-azirines with ethyl 2-cyano-2-diazoacetate or 2-diazo-3,3,3-trifluoropropionate provides an easy access to 2H-1,3-oxazines and 1H-pyrrol-3(2H)-ones. These compounds can be selectively prepared from the same starting material using temperature as the only varied parameter. The 2-azabuta-1,3-diene intermediate, a common precursor for both heterocyclic products, isomerizes into 2H-1,3-oxazine under kinetic control, while 1H-pyrrol-3(2H)-one is the sole product of the reaction at elevated temperatures. According to DFT-calculations a one-atom oxazine ring contraction involving ring-opening to a 2-azabuta-1,3-diene intermediate, followed by a 1,5- and 1,2-prototropic shift leads to the consecutive formation of imidoylketene and azomethine ylide, which then further undergo cyclization to the pyrrole derivative. 相似文献