共查询到20条相似文献,搜索用时 15 毫秒
1.
Tectorigenin and irigenin are biologically active isoflavones of Belamcanda chinensis (L.) DC. Previous studies indicated that both compounds could be metabolized in vivo; however, the kinetic parameters of enzymes involved in the metabolization of tectorigenin and irigenin have not been identified. The aim of this study was to investigate UGTs involved in the glucuronidation of tectorigenin and irigenin and determine enzyme kinetic parameters using pooled human liver microsomes (HLMs) and recombinant UGTs. Glucuronides of tectorigenin and irigenin were identified using high-performance liquid chromatography (HPLC) coupled with mass spectrometry and quantified by HPLC using a response factor method. The results showed that tectorigenin and irigenin were modified by glucuronidation in HLMs. One metabolite of tectorigenin (M) and two metabolites of irigenin (M1 and M2) were detected. Chemical inhibition and recombinant enzyme experiments revealed that several enzymes could catalyze tectorigenin and irigenin glucuronidation. Among them, UGT1A1 and UGT1A9 were the primary enzymes for both tectorigenin and irigenin; however, the former mostly produced irigenin glucuronide M1, while the latter mostly produced irigenin glucuronide M2. These findings suggest that UGT1A1 and UGT1A9 were the primary isoforms metabolizing tectorigenin and irigenin in HLMs, which could be involved in drug–drug interactions and, therefore, should be monitored in clinical practice. 相似文献
2.
3.
KBrO3—KBr紫外分光光度法测定痕量水杨酸 总被引:5,自引:0,他引:5
研究了HCl溶液中KBrO3-KBr紫外分光光度法测定水杨酸的条件,建立了测定痕量水杨酸的新方法。结果表明,在0.6mol/LHCl,3*10^-5mol/LKBrO3,5*10^-4mol/LKBr,6*10^-4mol/lKI溶中测定水杨酸,其线性范围为0.2-4.0mg/L,表观摩尔吸光系数为1.9*10^-4L.mol^-1.cm^-1,Sandell为7.3μg/cm^2。sg if e 相似文献
4.
血清中阿斯匹林和水杨酸浓度的快速高效液相色谱法测定 总被引:3,自引:0,他引:3
A fast method for determining concentrations of aspirin and salicylic acid in serum using reversedphase high performance liquid chromatography with Spherisorb C18 column,MeOH i H2O: n-BuOH : H3PO4 (300:200 :10 :0. 5,volume ratio) eluent and UV-237 detector was eatablished. The linear range of the method is 1-20μg/mL (r=0. 9996)for aspirin and 2 30μg/mL (r=0. 9981) for salicylic acid. The averagerecoveries are 96. 0 %-106. 0% and 92. 0%-119.5% ,respectively. 相似文献
5.
建立了原料乳中水杨酸的检测方法.样品经乙腈提取,采用超高效液相色谱进行测定.在ZORBAX Eclipse Plus C18反相柱上分离,梯度洗脱,流动相为磷酸水和磷酸甲醇,DAD检测.水杨酸的线性范围为0.5~20 μg/mL,相关系数平方(R2)大于0.995,检出限为0.5 mg/kg,定量限为1.25 mg/kg,3个不同水平的加标回收率为88.0%~118.9%,相对标准偏差不大于10%.方法具有操作简单、重现性好、灵敏度高、杂质干扰小等特点,可以用于原料乳中水杨酸的检测. 相似文献
6.
Kalbasi Roozbeh Javad Massah Ahmad Reza Zamani Farzad Javaherian Naghash Hamid 《中国化学》2010,28(3):397-403
TiO2‐ZrO2 (1/1)‐surf with Ti and Zr molar ratio of 1/1 was prepared with surfactant through a sol‐gel method. The optimum experimental condition was investigated for nitration of salicylic acid. Then, a number of nitration reactions were carried out with a variety of aromatic compounds in the optimum condition. The 25 wt% H3PO4/TiO2‐ZrO2 (1/1)‐surf catalyst showed good selectivity and yield in a short time for the nitration of salicylic acid and some other aromatic compounds. 相似文献
7.
建立测定阿司匹林合成过程中水杨酸、阿司匹林含量和转化率的分析模型。采用紫外多波长扫描乙醇溶液中阿司匹林和水杨酸的紫外光谱,建立水杨酸和阿司匹林紫外光谱的向量长度与其质量浓度的标准曲线,通过斜投影算法分离出待测样本中阿司匹林和水杨酸的紫外光谱。计算光谱向量长度,代入标准曲线得到待测样本中阿司匹林和水杨酸的含量。实验结果表明,水杨酸、阿司匹林的质量浓度与其紫外光谱的向量长度呈良好的线性关系,线性范围分别为2.00~40.00,10.00~200.00μg/m L。水杨酸和阿司匹林测定结果的相对标准偏差为0.16%~2.19%(n=5),加标回收率在93.3%~106.9%之间。该方法快速简便、准确可靠,可满足阿司匹林和水杨酸的同时测定及反应过程中水杨酸转化率监测要求。 相似文献
8.
高效液相色谱法测定皮炎宁酊中间苯二酚和水杨酸的含量 总被引:16,自引:0,他引:16
采用高效液相色谱法测定了皮炎宁酊中间苯二酚和水杨酸的含量。色谱柱为HypersilODS柱,流动相为V(甲醇)∶V(水)∶V(乙酸)=50∶50∶0.9。检测波长为285nm。在此条件下,间苯二酚和水杨酸可与其降解产物及杂质完全分开。 相似文献
9.
10.
高效液相法测定注射用赖氨匹林中的阿司匹林及游离水杨酸 总被引:11,自引:0,他引:11
建立了高效液相法 (HPLC)同时测定注射用赖氨匹林中阿司匹林和游离水杨酸含量的方法。采用的柱为HypersilBDSC18柱 ,流动相为甲醇 水 冰醋酸 (体积比为 35∶6 5∶3) ,检测波长为 2 80nm。阿司匹林和水杨酸的质量浓度分别为 0 0 2 8g/L~ 0 14 1g/L和 0 77mg/L~ 3 85mg/L时线性关系良好 ,其线性相关系数分别为0 9999和 0 9998;加样回收率分别为 99 2 7% (RSD =0 8% )及 99 6 1% (RSD =1 3% )。 相似文献
11.
12.
Fatemeh F.BAMOHARRAM Majid M.HERAVI Javad EBRAHIMI Ali AHMADPOUR Mojtaba ZEBARJAD 《催化学报》2011,32(5):782-788
An efficient and environmentally benign procedure for the catalytic esterification of salicylic acid with aliphatic alcohols, CnH2n+1OH(n=1-5)and benzylic alcohols,RC6H4CH2OH(R=H,NO2,OCH3,Br,Cl,Me)was developed using nano-SiO2-supported Preyssler heteropolyacid both under thermal conditions and microwave irradiation.Silica nanostructures were obtained through a sol-gel method and were characterized by transmission electron microscopy and powder X-ray diffraction.The effects of various parameters such as solvent type,molar ratio of substrates,Preyssler heteropolyacid loading on silica,catalyst amount,temperature,and reaction time were studied and the optimum conditions were obtained.It has been found that the catalyst with 30 wt%loading is highly active and shows high yields in esterification reactions.The use of nano-SiO2-supported Preyssler heteropolyacid coupled with microwave irradiation allows a solvent-free,rapid(3 min),and high-yielding reaction.This catalyst can be easily recovered and reused for many times without a significant loss in its activity. 相似文献
13.
Tomonobu Uchino Yuichi Tozuka Toshio Oguchi Keiji Yamamoto 《Journal of inclusion phenomena and macrocyclic chemistry》2002,43(1-2):31-36
The inclusion compounds of linear amylose and salicylic acid analogues preparedby sealed-heating were evaluated by using powder X-ray diffraction and infraredspectroscopy. Sealed-heating of amylose and either salicylamide or benzoic acidinduced the amylose structural change from the 61- to the 71-helix structure, while sealed-heating with o-toluic acid, o-chlorobenzoic acid and o-nitrobenzoic acid induced the change from the 61- to the 81-helix structure. In contrast to theresults in the salicylic acid system, the amylose helix type of sealed-heated samplewas not varied by the load of the guest compound, but was fixed only by the guestspecies. No change of the helical structure of amylose depending on the amylose molecular weight was observed. From the comparison of inclusion compound formation among different guest systems, it was found that the higher vapor pressure at 100 °C of the guest resulted in faster inclusion compound formation. The vapor pressure of the guest compound would be an important factor affecting the progress of inclusion compound formation. 相似文献
14.
利用水杨酸、阿司匹林、乙酸酐和乙酸的多波长紫外光谱作为本底光谱库和样本光谱库,建立一种基于子空间夹角判据的快速定量分析阿司匹林合成体系中水杨酸和阿司匹林的方法。水杨酸、阿司匹林质量浓度范围分别在1.16~34.684,9.84~188.928μg/mL内,所测得水杨酸和阿司匹林检验集的R分别为0.999 7和0.999 1,RMSE分别为0.509 5,2.624 0μg/mL,效果优于普通波峰点最小二乘法和偏最小二乘法。水杨酸和阿司匹林的加标回收率在94.01%~106.44%之间,测定结果的相对标准偏差分别为3.45%和4.80%(n=7)。该方法可用于监测阿司匹林合成体系中的水杨酸和阿司匹林。 相似文献
15.
Ledy De-la-Cruz-Martínez Constanza Duran-Becerra Martin Gonzlez-Andrade Jos C. Pez-Franco Juan Manuel Germn-Acacio Julio Espinosa-Chvez J. Martin Torres-Valencia Jaime Prez-Villanueva Juan Francisco Palacios-Espinosa Olivia Soria-Arteche Francisco Corts-Benítez 《Molecules (Basel, Switzerland)》2021,26(14)
Regulating insulin and leptin levels using a protein tyrosine phosphatase 1B (PTP1B) inhibitor is an attractive strategy to treat diabetes and obesity. Glycyrrhetinic acid (GA), a triterpenoid, may weakly inhibit this enzyme. Nonetheless, semisynthetic derivatives of GA have not been developed as PTP1B inhibitors to date. Herein we describe the synthesis and evaluation of two series of indole- and N-phenylpyrazole-GA derivatives (4a–f and 5a–f). We measured their inhibitory activity and enzyme kinetics against PTP1B using p-nitrophenylphosphate (pNPP) assay. GA derivatives bearing substituted indoles or N-phenylpyrazoles fused to their A-ring showed a 50% inhibitory concentration for PTP1B in a range from 2.5 to 10.1 µM. The trifluoromethyl derivative of indole-GA (4f) exhibited non-competitive inhibition of PTP1B as well as higher potency (IC50 = 2.5 µM) than that of positive controls ursolic acid (IC50 = 5.6 µM), claramine (IC50 = 13.7 µM) and suramin (IC50 = 4.1 µM). Finally, docking and molecular dynamics simulations provided the theoretical basis for the favorable activity of the designed compounds. 相似文献
16.
17.
The reaction between salicylic acid and dialkyl phosphite was traced by electrospray ionization mass spectrometry and ^31P NMR. All reactants, unstable intermediates and products were detected. The mechanism was proposed based on ESI-MS results and ^31P NMR profiles. 相似文献
18.
A rapid and simple high performance liquid chromatographic method coupled with tandem mass spectrometry (LC–MS–MS) via electrospray
ionization (ESI) has been developed and validated to separate and simultaneously quantify sodium ferulate (SF), salicylic
acid (SA), cinnarizine (CIN) and vitamin B1 (VB1) in human plasma. Gemfibrozil (GEM) was used as the internal standard (IS)
for SF and SA, whereas lomerizine (LOM) was used as the IS for CIN and VB1. The plasma samples were prepared by one-step protein
precipitation followed by an isocratic elution with 10 mM ammonium acetate buffer (pH = 5.0): acetonitrile (35:65, v/v,) on an Agilent Zorbax SB-CN column (150 mm × 2.0 mm ID, 5 μm). The precursor and product ions of these drugs were monitored
on a triple quadrupole mass spectrometer, operating in the selected reaction monitoring mode (SRM) with polarity switch, in
the negative-ion mode for SF, SA and GEM, in the positive-ion mode for CIN, VB1 and LOM. The method was validated over the
concentration range of 1.5–1,000 ng mL−1 for SF, 20–5,000 ng mL−1 for SA, 2–500 ng mL−1 for CIN, 1–30 ng mL−1 for VB1. The intra- and inter-batch precisions were less than 15% of the relative standard deviation. The recoveries for
analytes and IS achieved from spiked plasma samples were consistent and reproducible. The validated LC–MS–MS method has been
successfully applied to the pharmacokinetic study of sodium ferulate and aspirin capsule in healthy volunteers. 相似文献
19.