Device for filling of small diameter capillaries with stationary phase solutions in liquefied gases

This paper describes a high pressure device for filling small diameter capillaries with stationary phase solutions is described. A liquid is forced into the capillary column with the help of high pressure syringe whose needle (provided with a side opening) is tightened in a PTFE seal. The device allows use of liquefied gas as solvent. A detailed procedure is given for filling the capillary with stationary phase solution. The performance of the device was evaluated by filling 12 m × 15 μm i. d. glass capillary with 6.5 % (w/v) SE-54.     

Combining chromatography and chemometrics for the characterization and authentication of fats and oils from triacylglycerol compositional data—A review

The characterization and authentication of fats and oils is a subject of great importance for market and health aspects. Identification and quantification of triacylglycerols in fats and oils can be excellent tools for detecting changes in their composition due to the mixtures of these products. Most of the triacylglycerol species present in either fats or oils could be analyzed and identified by chromatographic methods. However, the natural variability of these samples and the possible presence of adulterants require the application of chemometric pattern recognition methods to facilitate the interpretation of the obtained data. In view of the growing interest in this topic, this paper reviews the literature of the application of exploratory and unsupervised/supervised chemometric methods on chromatographic data, using triacylglycerol composition for the characterization and authentication of several foodstuffs such as olive oil, vegetable oils, animal fats, fish oils, milk and dairy products, cocoa and coffee.