Glycolipids of the woody verdure ofLarix sibirica

The compositions of the glycolipids of the main fractions of the woody verdure of the Siberian larch — needles and shoots — have been studied. The dynamics of the amounts of individual glycolipids in the course of vegetation have been established. The qualitative and quantitativae compositions of the fatty acids of the total glycolipids of the needles and shoots have been determined.Siberian Technological Institute, 660049, Krasnoyarsk, Prospekt Mira, 82. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 183–187, March–April, 1994.     

Fractionation and composition of the carbohydrates ofFicus carica

The fractional compositions of the carbohydrates of the four most promising varieties ofFicus carica L. — Smena, Kadota, Chumlakuri mtsvane, and Turetskii korichnevyi — have been investigated. The qualitative and quantitative monosaccharide compositions of the carbohydrates isolated have been determined. The bulk of the dry matter of the fruit consists of ethanol-soluble oligosaccharides — up to 74.53%. The composition of the residue obtained after the fractionation of the carbohydrates has been studied. It contains ligin bound to mineral elements.N. V. Lomonosov Odessa Technological Institute of the Food Industry. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 673–677, November–December, 1986.     

Polar lipids of cotton seeds and the products of their processing

The compositions and amounts of polar lipids in cotton seeds and the products of their processing have been investigated. Six glycolipids have been identified, the main ones being monogalactosyldiglyceride (MGDGs), digalactosyldiglycerides (DGDGs), and sterol glycosides. The fatty acid compositions of the neutral lipids and of the phospho- and glycolipids have been determined. In the glycolipids, saturated fatty acids — palmitic and stearic — predominated.Institute of Chemistry of Plant Substances, Uzbekistan Republic Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 194–198, March–April, 1993.     

Lipids of the fruit of Citrus unshiu

The compositions and the amounts of various groups of liposoluble compounds in the component elements of the fruits ofCitrus unshiu Marc. [satsuna variety of mandarin orange] — the flavedo, the albedo, the membrane, and the flesh — have been determined by chromatographic and chemical methods. About 30 groups of lipid compounds belonging to the classes of neutral lipids and glyco- and phospholipids have been identified and characterized. The fatty-acid compositions of the main classes of lipids have been studied. About 30 different carotenoids have been determined in the various elements of the satsuma orange.M. V. Lomonosov Odessa Technological Institute of the Food Industry. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 342–348, May–June, 1989.     

Phospholipid and fatty acid composition of Echinodermata. I. ClassOphiuroidea

The phospholipid compositions of five species of marine ophiuroids belonging to the classOphiuroidea have been investigated. It has been shown that the phosphatidylethanolamines contain almost solely ether bonds in the first position of the glycerophospholipids. The amount of the plasmalogen form in the phosphatidylethanolamines averages 89.8% (82.3–96.2%) and the amount of the alkyl form 9.8% (3.8–17.7%). The compositions of the fatty acids, fatty aldehydes, and alkyl ethers in the two main classes of phospholipids — phosphatidylethanolamine and phosphatidylcholine — have been studied. The main acids found were eicosatetraenoic and eicosapentaenoic, the total amount of which was 53%. It has been shown that the ophiuroids can be used as promising objects for the investigation of the metabolism of alkoxylipids and for their preparative isolation.Volga Basin Ecological Institute of the USSR Academy of Sciences, Tol'yatti. Translated from Khimya Prirodnykh Soedinenii, No. 5, pp. 547–551, September–October, 1986.     

Lipids of cottonseed kernels

The free, bound, and strongly bound lipids of the seed kernels of the cotton plant of variety 108-F have been studied. It has been shown that the free and bound lipids include 97.3% and 47.6% of neutral lipids, respectively. The polar lipids consist mainly of phospholipids and glycolipids. Two groups of glycolipids — sterol glycosides and glycosylglycerides — have been isolated and their compositions have been determined.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 435–438, July–August, 1983.     

Polysaccharides of the cell walls of fungi pathogenic for the cotton plant

The dynamics of the secretion of polysaccharides by fungi pathogenic for the cotton plant — Verticillium dahliae and Fusarium oxysporum — have been studied. An alkaline hydrolysate of the fungal cell walls has been obtained. Total fractions of the polysaccharides have been separated on Sephadex G-50 and Acrilex P-4, and their monosaccharide compositions have been determined.Institute of Microbiology, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 41 71 29. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 198–201, March–April, 1995. Original article submitted October 19, 1994.     

The seed oil of Catalpa. I

Summary 1. The fatty-acid compositions of the seed oils of catalpas cultivated in the Soviet Union have been studied.2. The oil content of the seeds is 21.2–36.7%, the refractive index 1.4905–1.5400, and the iodine number 184.8–201.7.3. The following fatty acids have been found in catalpa oil (%): palmitic — 1.3–4.3; stearic — 1.1–2.5; heneicosanoic — 0.5–5.2; oleic — 5.5–9.8; linoleic — 39.6–50.3; linolenic — 0.4–1.8; and eleostearic — 32.8–46.2.4. The most accurate results for characterizing the degree of unsaturation of the fatty acids of the catalpa seed oils are given by Woburn's method of determining iodine numbers. The iodine numbers obtained experimentally agree with those calculated and consequently the addition of halogen to the system of double bonds takes place at all the double bonds.5. The degree of unsaturation of the fatty acids of catalpa oil characterized by the iodine numbers and refractive indices increases in the sequence of species southern, northern, teas, Chinese and has a tendency to rise on passing to more northerly zones, which is one more piece of evidence confirming the correctness of S. L. Ivanov's climatic theory of the structure of fats.Kalinin Polytechnic Institute. Moscow Branch of the All-Union Scientific-Research Institute of Fats. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 331–337, May–June, 1977.     

The seed oils of some species of the family Cruciferae

Summary The physicochemical properties of the oils of the seeds of seven plants of the family Cruciferae —Cakile maritima, Sisymbrium altissimum, Sisymbrium Loeselii, Descurainia Sophia, Erysimum cheiranthoides, Brassica amoracioides andCrambe pontica — have been studied and their fatty acid compositions have been determined. The results obtained showed no marked differences from those given in the literature.Zaporozhe Medical Institute. Ukrainian Scientific-Research Institute of the Oils and Fats Industry. Zaporozhe Oils and Fats Combine. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 15–18, January–February, 1973.     

Comparative investigation of the molecular structures of the species-specific proteins of the seeds ofGossypium hirsutum andG. barbadense

The N-terminal amino acids and the amino acid compositions of four species-specific proteins of two species of the cotton plant —Gossypium hirsutum andG. barbadense — have been determined. Peptide maps of tryptic hydrolysates of these proteins have been obtained and compared. Individual tryptic peptides have been isolated and purified. The N-ends, amino acid compositions, and amino acid sequences of individual peptides have been determined. The N-terminal sections of the proteins ofG. hirsutum andG. barbadense as far as the 16th amino acid residue have been sequenced. A comparative analysis has been made of the peptides and proteins of these cottonplant species.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 40 64 75. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 858–864, November–December, 1997.     

Polyprenols and dolichols from the needles, needle-free shoots, and bark ofPinus sibirica

The presence of bioregulators of animal organisms — dolichols — has been established in the needles, needle-free shoots, and bark of the Siberian stone pine. The qualitative and quantitative compositions of the mixtures of the isoprenologues and polyprenols have been established by the HPLC method. In addition, known diterpene alcohols ((E,E,E)-geranylgeraniol and labd-8(17),13E-dien-15-ol) esterified with higher fatty acids have been identified in the bark.Novosibirsk Institute of Organic Chemistry, Siberian Division of the Russian Academy of Sciences. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 729–733, November–December, 1994.     

An investigation of cottonseed globulins. XXI. The 11S globulins of two varieties of the cotton plant

The 11S globulins of two varieties of the cotton plant — 108-F and Tashkent-1 — have been studied. It has been shown that the 11S globulins do not differ in quaternary structure and each consists of three subunits A, B, and C. It has been found that the subunits of these varieties differ in their amino acid compositions and the peptide maps of tryptic and chymotryptic hydrolysates.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 680–684, September–October, 1979.     

Neutral diterpenoids of oleoresins of five species of conifers of Transcarpathia

The compositions of the neutral diterpenoids of the oleoresins of five species of conifers growing in the Transcarpathia have been studied. It has been found that the oleoresins ofAbies alba M.,Larix decidua M., andPicea excelsa L. contain more than 50% of neutral diterpenoids. The group and qualitative compositions of the oxygen-containing diterpenoids have been determined. In the oleoresins ofAbies alba,Picea excelsa, andPicea abies tertiary alcohols — cis-abienol and isocembrol — predominate, while inLarix decidua the main component is the hydroxy ester larixyl acetate. Primary alcohols related to the resin acids have been found in all the oleoresins investigated. Features of the distribution of diterpenoids according to the species of conifers have been revealed. The results obtained are necessary for the chemotaxonomy of conifers of the family Pinceae.Novosibirsk Institute of Organic Chemistry, Siberian Branch, Academy of Sciences of the USSR. Translated from Khimiya Prirodynkh Soedinenii, No. 6, pp. 812–816, November–December, 1988.     

Neutral lipids of freon extracts of the fruit ofHippophaë rhamnoides

The composition of Freon extracts of the fruit of two samples of sea buckthorn has been studied — from the Western Pamir and from Biisk. The presence of 16 classes of lipids has been shown. The distribution of the fatty acid residues between the 2- and 1,3-positions of the triacyl glycerols of these extracts have been studied by the method of lipase hydrolysis, and their position-species compositions have been established.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 231–234, March–April, 1987.     

Lipids of the leaf vegetables Spinacea oleracea,Latuca sativa,and Rumex acetosa

The class compositions of the lipids of leaf vegetables —Spinacea oleracea (variety Ispolinskii),Latuca sativa (variety Kucheryavets Odesskii), andRumex acetosa (variety Odesskii-17) have been studied. The compositions of their NLs (17 groups of individual compounds were identified), GLs (nine groups of compounds were found), and PhLs (seven groups of compounds have been found) have been determined by physicochemical methods of analysis. The maximum amount of the total lipids and also of the PhLs was possessed by the spinach. Among the FAs of the leaf vegetables, USFAs predominated.M. V. Lomonosov Technological Institute of the Food Industry, Odessa. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 199–205, March–April, 1993.     

Production of lipid preparations. I. Isolation of natural phosphatidylcholines

The phospholipid compositions (qualitative and quantitative) are given of a wide range of natural sources — organs (brain, heart, liver, kidneys, spleen, lungs, pancreas) of two vertebrate animals (ox and pig) and 27 species of marine invertebrates of five types (coelenterates, arthropods, molluscs, echinoderms, and tunicates). Methods of isolating phosphatidylcholines from six sources — porcine brain and kidneys, a marine ascidian, a mussel, a starfish, and soybean phosphatides — are described and the compositions of the fatty acids of these phosphatidylcholines are given.Far Eastern State University, Vladivostok. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 214–220, March–April, 1987.     

Oils of the seeds of nine species of the genusCrambe

Summary The physicochemical properties of the oils of nine species of the genusCrambe have been studied and their fatty-acid compositions have been determined. The fatty acids of the oils contain not less than 20% of erucic acid. Its highest amounts are found in the oils ofC. abyssinica andC. pinnatifida — 57.01 and 47.37%, respectively.Zaporozh'e Medical Institute. Ukrainian Branch of the All-Union Scientific-Research Institute of the Oil and Fats Industry, Zaporozh'e. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 18–20, January–February, 1977.     

Extraction of C60 from fullerene-containing carbon soots

Extraction of fullerene-containing carbon soots with various organic solvents has been studied at room temperature. Yields and compositions of toluene-soluble carbon soot fractions have been determined. Extracts of different carbon soot samples treated according to the toluene—trichlorobenzene—nitrobenzene scheme are studied.Translated fromIzvestiya Akademii Nauk. Seriya Khimicheskaya, No. 7, pp. 1223–1225, July, 1995.The authors are grateful to E. B. Yagubskii and I. S. Krainskii for helpful discussion and help in the work.The work was financially supported by the Russian Foundation for Basic Research (Project No. 93-03-18705).     

Composition of the phospholipids of the cotton plant Gossipium barbadense

Summary 1. The fatty-acid compositions of the oil, the total phospholipids, and the main groups of phospholipids of the seed kernels of the fine-fibered cotton plant of variety 5904-I have been determined. The complete qualitative identity of the acids present in them and quantitative similarity of the acids in the oil and in the total phospholipids have been shown: in the degree of increasing saturation the phospholipids form the following sequence: phosphatidylcholines, phosphatidylethanolamines, phosphatidylinositols.2. It has been established by enzymatic hydrolysis with phospholipase A₂ of kufi venom that the unsaturated fatty acids are present in position of the PCh, PE, and PI molecules — to the extent of 90.6%, 96.5%, and 85.4%, respectively.3. From the position distribution of the fatty acids in the phospholipids, their possible molecular compositions have been calculated.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 693–697, November–December, 1975.     

Chemical synthesis and enzymatic assembly of fragments of the DNA coding immunodominant epitopes of human immunodeficiency virus

More than twenty 13- to 55-membered oligo(poly)nucleotides have been synthesized by the H-phosphonate solid-phase method in the manual variant using an original procedure. From the oligonucleotides obtained, seven DNA duplexes coding immunodominant epitopes of HIV-1 proteins have been formed: 598–609 and 737–748 gp41, 91–115 and 105–115 gag, and 940–951 pol and a number of its mutants. The ligase assembly and polymerization of the DNA duplexes obtained has been carried out. The efficiency of ligation amounted to from 60 to 90%. The possibility of their directed polymerization was determined by the flanking of the duplexes by partially completed half-sites of restrictases BamHI and XhoI.Abbreviations adopted: HIV-1 — human immunodeficiency virus, type 1; (+)-chain — the coding chain — and (–)-chain the chain complementary to the coding chain of the DNA duplex; CPG — controlled-pore glass; PAAG — polyacrylamide gel; Py — pyridine.D. I. Ivanovskii Institute of Virology, USSR Academy of Medical Sciences, Moscow. V. A. Engel'gardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 393–399, May–June, 1990.