共查询到19条相似文献,搜索用时 66 毫秒
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偏振共焦扫描显微镜的成像特性研究 总被引:3,自引:0,他引:3
讨论了共焦扫描激光显微镜的纵向分辨率和信噪比对三成像的影响,给出了纵向分辨主极限判据,提出了旨在提高信噪比进而获取强表面反或弱散射物体以及偏振物体的显微结构图象的偏振共振扫描激光显微镜。 相似文献
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激光共焦扫描显微镜的分辨率及像差补偿 总被引:1,自引:0,他引:1
从激光共焦扫描显微镜的基本原理出发,深入讨论激光共焦扫描显微镜的深度分辨率和横向分辨率,同时研究三维成像的像差补偿问题。 相似文献
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In order to further improve the performance of a confocal microscope (CM) used for measurement of surface profiles and 3D microstructures, a shaped annular beam heterodyne confocal measurement method based on annular pupil filter technique and reflection confocal microscopy, is proposed to expand the measurement range and to improve the defocused property of CM. The approach proposed uses a confocal dual-receiving light path arrangement and a heterodyne subtraction of two signals received from detectors with axial offset to enable CM to be used for bipolar absolute measurement and to improve the defocused property of CM, and it uses the annular pupil filter technique to produce a binary optical shaped annular beam, which expands the measurement range by expanding the full-width at half-maximum of intensity curve received from two detectors in a heterodyne confocal microscopy system. Theoretical analyses and experimental results indicate that a shaped annular beam heterodyne microscope has a measurement range expanded from 4 to 14 μm, achieved an axial resolution of 2 nm and improved the defocused property, when ε=0.5 and NA=0.65. It can be therefore concluded that the shaped annular beam heterodyne confocal measuring method proposed is a new approach to ultraprecision measurement of surface profiles and 3D microstructures. 相似文献
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一种提高共聚焦显微镜信噪比算法的研究 总被引:1,自引:1,他引:0
基于共焦显微镜的成像特点,建立了Kalman滤波算法的理论模型,把Kalman滤波方法引入到系统中,提出一种基于图像像素的Kalman滤波算法,并实现了实时化的Kalman滤波器。实验结果表明:该算法能够有效地提高共焦显微镜信噪比,但是以牺牲时间为代价,提高系统分辨率的根本方法还是要着重考虑优化成像系统光路和探测电路。 相似文献
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差动共焦式纳米级光聚焦探测系统的研究 总被引:5,自引:0,他引:5
为解决纳米级大范围的非接触测量问题 ,提出了基于差动式共焦显微技术的光聚焦探测系统。介绍了系统的工作原理和结构装置 ,对系统中的关键技术进行了优化设计 ,通过对共焦光路的差动设计 ,可以有效地抑制光源的噪声和漂移对测量结果的影响。初步实验结果表明 ,该系统的轴向分辨率可达 2 nm 相似文献
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Although confocal fluorescence microscopes are widely used in biology and have been proven to be promising diagnostic tools in dermatologic diagnostics, they are at present uncommon in medical practice. This is mainly due to high costs of acquisition and their large and complex outline. With the integration of a MEMS scanner we present a demonstration system of a confocal fluorescence laser scanning microscope which is affordable and portable. It has a field of view of 500 μm × 500 μm and is mainly composed of off-the-shelf components. 相似文献
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扫描探针显微镜主要包括扫描隧道显微镜和原子力显微镜,其利用尖锐的针尖逐点扫描样品,可在原子和分子尺度上获取表面的形貌和丰富的物性,改变了人们对物质的研究范式和基础认知。近年来,qPlus型高品质因子力传感器的出现将扫描探针显微镜的分辨率和灵敏度推向了一个新的水平,为化学结构、电荷态、电子态、自旋态等多自由度的精密探测和操控提供了前所未有的机会。文章首先简要介绍原子力显微镜的发展历史和基本工作原理,然后重点描述qPlus型原子力显微镜技术的优势及其在单原子、单分子和低维材料体系中的应用,最后展望该技术的未来发展趋势和潜在应用。 相似文献
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激光扫描声学显微镜光学系统的设计 总被引:1,自引:0,他引:1
半导体激光器具有半导体和固体器件的许多优点:结构紧凑、效率高、价格便宜、长寿命,在许多方面优于气体激光器,因此在一些测量领域有较好的应用前景。本文基于棱镜对光的一维压缩及新型半导体激光器,对激光扫描声学显微镜的光学系统进行了新的设计,给出了相关数据及具体技术指标,实验证实其光学性能满足要求。整个光学系统具有结构紧凑、可靠性高、使用方便等特点,现已用于激光扫描声学显微镜定型设计。 相似文献