胆结石的FT－Raman光谱研究

胆结石的ＦＴ－Ｒａｍａｎ光谱研究徐智勇，李维红，王立波，许振华，吴瑾光（北京大学化学与分子工程学院，稀土材料化学与应用国家重点实验室北京１００８７１）ＴｈｅＩｄｅｎｔｉｆｉｃａｔｉｏｎｏｆＴｈｒｅｅＤｉｆｆｅｒｅｎｔＴｙｐｅｓｏｆＧａｌｌｓｔｏｎｅｓ...     

稀土离子与二棕榈酰磷脂酰胆碱相互作用的FT—IR研究

利用傅里叶变换红外光谱法研究了稀土离子Ｓｍ＾３＋，Ｇｄ＾３＋，Ｅｒ＾３＋和Ｙｂ＾３＋与二棕榈酰磷脂磷胆碱脂质体的相互作用，结果表明：稀土离子间磷脂极性头部的ＰＯ＾－２和Ｎ＾＋（ＣＨ３）３基闭间有较强的静电作用，而且这些作用随着稀土子摩尔比例的增大而加强，稀土离子与磷脂相互作用程度按稀土离子原子序数增大即Ｓｍ＾３＋＜Ｇｄ＾３＋＜Ｙｂ＾３＋的顺序排列。     

一些异核双金属有机化合物的FT－Raman光谱

一些异核双金属有机化合物的ＦＴ－Ｒａｍａｎ光谱郭建华，谭，聂崇实（中国科学院上海有机化学研究所上海市２０００３２）ＦＴ－ＲａｍａｎＳｐｅｃｔｒａｏｆＳｏｍｅＨｅｔｅｒｏｂｉｎｕｃｌｅａｒＭｅｔａｌｌｏｒｇａｎｉｃＣｏｍｐｏｕｎｄｓ￥ＪｉａｎＨｕａＧｕ...     

辣根过氧化物酶（HRP）水溶液体系的FT－Raman光谱研究

辣根过氧化物酶（ＨＲＰ）水溶液体系的ＦＴ－Ｒａｍａｎ光谱研究姜世梅，李正强（吉林大学分子光谱与分子结构开放实验室长春１３００２３）王顺光（中科院上海生物化学研究所上海２０００３１）刘伟（吉林大学酶工程国家重点实验室长春１３００２３）ＦＴ－ＲａｍａｎＳ...     

BR－PVA－Ag膜热稳定性的Raman光谱研究

本文研究了ＢＲ－ＰＶＡ－Ａｇ膜的Ｒａｍａｎ光谱随温度的变化，结果表明：这种组装膜在８０℃以下是非常稳定的，温度在８Ｏ－９５℃范围内它仍部分具有功能特性，温度超过１００℃，ＢＲ已经变性。     

Interaction of Scopolamine and Cholesterol with Sphingomyelin Bilayers by FT-Raman Spectroscopy

The interaction of scopolamine and cholesterol with sphingomyelin bilayers has been investigated by FT-Raman spectroscopy in head-group region (600-1000 cm^-1), the C-C stretching (1000-1200 cm^-1), CH₂ deformation (1400-1500 cm^-1) and the C-H stretching (2800-3000 cm^-1) mode regions. The results indicate that scopolamine and cholesterol do not change the conformation of O-C-C-N⁺ backbone in the choline group of sphingomyelin bilayers, the polar headgroup is still extending parallel to the bilayer surface and 0-C-C-N⁺ group is still in its gauche conformer. Scopolamine and cholesterol lower the order of the interface, the interchain, CH₂ crystal lattices and the lateral chain-chain packing, and increase their fluidity.     

新型镧配合物的合成与荧光性质

在氮气保护下,以无水乙醇为溶剂,制备了硝酸镧与4-氨基-1,2,4-三氮唑缩对二甲氨基苯甲醛(L)配合物,单晶结构分析确定其组成为LaL_3(NO_3)_3·(H_2O)_2·(C_2H_5OH)_2,配合物中La~(3+)分别与3个硝酸根、2个水分子和2个分子乙醇中的氧原子配位,配位数为10,并与4-氨基-1,2,4-三氮唑缩对二甲氨基苯甲醛以氢键形式结合形成立体结构分子.通过元素分析、红外光谱、固体荧光光谱等手段对合成的配体及配合物进行了表征.实验结果表明,4-氨基-1,2,4-三氮唑缩对二甲氨基苯甲醛是一种多晶粉末状的发光材料,固体荧光谱图显示荧光发射峰在451 nm处,为蓝色荧光,色纯度高,荧光量子效率高,而合成的配合物的荧光发射峰则红移至464 nm左右,同时荧光强度显著增强近一倍.     

诺氟沙星与DNA的拉曼光谱研究

报道了诺氟沙星(NFX)及诺氟沙星胶囊内容物的FT-Raman光谱和在银胶基底上的表面增强拉曼光谱(SERS),归属了各个振动;研究了诺氟沙星与DNA的相互作用的SERS,结果表明:胶囊内容物的拉曼光谱图与对照品的拉曼光谱图的特征振动峰:C-F键的伸缩振动,C=C伸缩振动,O-C-O的对称伸缩振动峰值未发生变化,发生变化主要是分子骨架振动峰,诺氟沙星胶囊的辅料对拉曼光谱无实质影响,可建立拉曼光谱法检测诺氟沙星药物的分析方法;诺氟沙星可以在没有金属离子的存在下与DNA直接作用,与DNA相互作用的主要键合模式是插入作用,NFX分子中的平面结构插入DNA的双螺旋碱基平面,为深入了解喹诺酮类抗生素的抗菌机理提供可靠依据.     

Gd3+,Yb3+和Tm3+共掺杂氟化物纳米晶中Yb3+-Tm3+-Gd3+间的能量传递

用水热法合成了Y_0.8-x-yF₃∶Gd_x³⁺,Yb_0.2³⁺,Tm_y³⁺纳米晶的上转换发光材料。在典型的Y_0.595F₃∶Gd_0.200³⁺,Yb_0.200³⁺, Tm_0.005³⁺纳米微晶中,在980 nm激光激发下,观察到了Tm³⁺的紫外、紫色上转换发射明显增强和来自于Gd³⁺的⁶D_9/2、⁶I_J、⁶P_5/2及⁶P_7/2能级到基态⁸S_7/2能级的紫外发射。通过比较Y_0.8-x-yF₃∶Gd_x³⁺ ,Yb_0.2³⁺,Tm_y³⁺纳米晶样品的上转换发光性质以及Tm³⁺和Gd³⁺中一些激发态的能级寿命,借助于能级图描述了Yb³⁺-Tm³⁺-Ga³⁺之间的有效的能量传递过程。     

拉曼光谱研究乳铁蛋白及其肽段与DPPC、DPPG脂质体的相互作用

利用拉曼光谱研究了乳铁蛋白及其肽段Lactofcrrcin B4-14和Lactoferrampin与DPPC、DPPG脂质体的相互作用.通过分析作用前后两种磷脂拉曼光谱的变化,探讨了乳铁蛋白及其肽段与磷脂的作用模式,为阐明乳铁蛋白及其肽段抗菌机理提供了重要依据.拉曼光谱显示,乳铁蛋白及其肽段加入中性磷脂DPPC脂质体...     

Interaction of Cholesterol with Dimethyl and Diethyl Sulfoxides

The complexing of cholesterol with dimethyl and diethyl sulfoxides in carbon tetrachloride is investigated by the method of IR spectroscopy. It is established that the hydrogen bond is formed between the oxygen of the sulfoxide group of sulfoxides and the hydrogen of the hydroxyl group of cholesterol. The equilibrium constants (K _eq) of the complexes of cholesterol with dimethyl sulfoxide (K _eq = 93 liters/mole) and diethyl sulfoxide (K _eq = 98 liters/mole) at 23°C are determined.     

The Interaction of Serum Albumin with Cholesterol Containing Lipid Vesicles

In this paper, the interaction of both human blood serum (the primary fraction of which is serum albumin) and pure human serum albumin (HSA) with surface immobilised lipid vesicles was measured by combined Surface Plasmon Resonance (SPR) and Surface Plasmon enhanced Fluorescence (SPEFS), and fluorescence microscopy. It was found that both blood serum and HSA showed specific binding to vesicles which contained cholesterol, resulting in increased membrane permeability and release of encapsulated fluorescent dye. This effect was not seen with heat inactivated blood serum, heat inactivated HSA or in vesicles not containing cholesterol. These results suggest that HSA may have a physiological role over and beyond that of fatty acid carrier, possibly acting to regulate vascular endothelial cell cholesterol concentration.     

Eu~(3+)作探针研究铋铕共掺硼硅酸盐玻璃光学特性

利用高温烧结法制备了铕/铋铕共掺硼硅酸盐玻璃系列样品,测量了样品的激发光谱、发射光谱和声子边带谱,利用Eu3+离子作为探针进一步研究了敏化剂Bi3+的掺入对Eu3+发光的影响.结果表明:本文制作的玻璃样品的电子-声子耦合系数比以往报道过的硼铅等玻璃材料的值都要小,但在硼硅酸盐玻璃中掺入Bi3+会使Eu3+的无辐射跃迁概率增加;Bi3+对Eu3+有敏化作用,Bi3+的掺入使材料的共价性增强,对称性降低,这又使得Eu3+的发光整体变强.所以,在硼硅酸盐玻璃体系中,Eu3+发光的增强不仅仅是由于Bi3+对Eu3+的能量传递,而是以上各因素综合作用的结果.     

BLM成膜液中胆固醇与卵磷脂相互作用的光谱学研究

结合胆固醇与卵磷脂的拉曼光谱,分析了BLM成膜液中胆固醇与卵磷脂的相互作用。得出膜液中胆固醇与卵磷脂的最佳配比为1∶4。室温下,电化学实验的结果表明,以此种比例配置的膜液使膜的稳定性得到提高,导电率减小。     

NMR and FT-Raman Studies on the Interaction of Lanthanide Ions with Sphingomyelin Bilayers

The interactions of lanthanide ions with sphingomyelin bilayers have been studied by using 2D NOESY spectroscopy and FT-Raman spectroscopy methods. The results indicate that lanthanide ions, as well as divalent calcium, combine mainly to the phosphate group in the polar headgroup and do not change the conformation of O-C-C-N⁺ backbone in the choline group of sphingomyelin bilayers. The polar headgroup is still extending parallel to the bilayer surface and O-C-C-N⁺ group is still in its gauche conformer.