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1.
Direct conversion of isothermal to temperature programmed indices is not possible. In this work it is shown that linear temperature programmed retention indices can only be calculated from isothermal retention data if the temperature dependence of both the distribution coefficients and the column dead time are taken into account. Procedures are described which allow calculation of retention temperatures and from these, accurate programmed retention indices. Within certain limits the initial oven temperature and programming rate can be chosen freely. The prerequisite for this calculation is the availability of reliable isothermal retention data (retention times, retention factors, relative retention times, or retention indices) at two different temperatures for one column. The use of compiled isothermal retention indices at two different temperatures for the calculation of retention temperatures and thus temperature programmed indices is demonstrated. For the column for which programmed retention indices have to be determined, the isothermal retention times of the n-alkanes and the column dead time as a function of temperature have to be known in addition to the compiled data for a given stationary phase. Once the programmed retention indices have been calculated for a given column the concept allows the calculation of temperature programmed indices for columns with different specifications. The characteristics which can be varied are: column length, column inner diameter, phase-ratio, initial oven temperature, and programming rate. 相似文献
2.
从双多阶程升保留时间预测恒温保留指数可行性研究 总被引:12,自引:2,他引:10
由于程序升温保留值受多种操作条件的影响,缺乏数据的共用性,作者提出了根据组分双多阶程序升温保留时间预测其恒温保留指数的方法。以烃类化合物对象,从理论和实验两方面论证了该法的可行性。 相似文献
3.
Retention indices were determined for a homologous series of n-dialkyl sulphides on three stationary phases (SE-30, OV-17 and XE-60) under isothermal and linear temperature programming conditions. Under these two different GC conditions, equations were derived for each of the three stationary phases which showed the dependence of retention index on the number of carbon atoms and the boiling points for a homologous series of n-dialkyl sulphides. The equation for the correlation isothermal retention index was shown to be applicable to the identification of n-dialkyl sulphides using linear temperature programming. It was found that the GC behaviour of n-dialkyl sulphides makes these compounds suitable for use as a standard series instead of n-alkanes for the calculation of retention indices in GC analysis in which detectors insensitive to n-alkanes are employed. The use of the homologous series of n-dialkyl sulphides for the calculation of sulphide retention indices can be great practical importance in the microanalysis of natural compounds. We have used this method successfully in the analysis of pesticides containing S-atoms. 相似文献
4.
Conversion of Kováts retention indices on a given stationary phase into the thermodynamic parameters of compounds on a given column leads to a simplified method for retention simulation in isothermal, linear, and multi-ramp temperature programmed capillary gas chromatography. The influence of numerical methods used in the computation, the temperature coefficient of Kováts indices, and the experimental factors such as isothermal temperatures selected in the measurement of n-alkanes, column characterization and sample overloading, on the reproducibility and accuracy of simulation were discussed and examined. When the column used is properly characterized, the error between the simulated values and the experimental data is within ± 0.5 index unit or less than ± 1% of retention time. 相似文献
5.
Summary A method is presented for the calculation of retention indices in linear programmed-temperature gas chromatography (LPTGC)
on the basis of isothermal retention data and the operating conditions (initial temperature, programming rate, gas flow velocity)
of the run.
Part IV of a series; parts I, II and III see refs. [1], [2] and [3]. 相似文献
6.
A relationship between the Kováts and Lee retention indices was obtained for dimethylsilicone stationary phase and isothermal conditions. The temperature dependence of the retention indices was taken into account. Based on numerous experimental data for dimethylsilicone stationary phase, parameters of the linear temperature dependence of the Kováts indices were determined for the reference compounds of the Lee scale. 相似文献
7.
Summary Thermodynamic characteristics determined from retention data measured under isothermal conditions were used for the calculation of retention indices of a number of model compound. The minimum retention index difference required for the identification of two adijacent compounds as well the temperature dependency of retention index are discussed. The possibility of alculating thermodynamic characteristics from retention indices published in the literature was also investigated.Dedicated to Professro J. F. K. Huber on the occasion of his 60th birthday. 相似文献
8.
Programmed temperature retention indices (PTRIs) calculated according to the equations of Van den Dool and Kratz, Golovnya and Uraletz, and Erdey et al. (also referred to as Antoine's integrated equation) are used in this work. Precalculation of isotherm retention indices from the results of a linearly programmed temperature GC is also presented. Deviations between experimental and calculated isothermal retention indices are below 2 retention index units. A relative "volatility" retention index is defined, as a function of the "volatilities"of the solute and the bracket reference n-alkanes. The comparison of the "volatility" retention indices with the PTRIs obtained with the other above equations shows absolute deviations of up to 4 retention index units. Based on an earlier "equivalent" temperature concept and on Tekler's proviso, a novel way for the utilization of Sadtler's retention index database, which takes advantage of the 3 data supplied by the library, is proposed. 相似文献
9.
10.
Stein SE Babushok VI Brown RL Linstrom PJ 《Journal of chemical information and modeling》2007,47(3):975-980
We have constructed a group contribution method for estimating Kováts retention indices by using observed data from a set of diverse organic compounds. Our database contains observed retention indices for over 35,000 different molecules. These were measured on capillary or packed columns with polar and nonpolar (or slightly polar) stationary phases under isothermal or nonisothermal conditions. We neglected any dependence of index values on these factors by averaging observations. Using 84 groups, we determined two sets of increment values, one for nonpolar and the other for polar column data. For nonpolar column data, the median absolute prediction error was 46 (3.2%). For data on polar columns, the median absolute error was 65 (3.9%). While accuracy is insufficient for identification based solely on retention, it is suitable for the rejection of certain classes of false identifications made by gas chromatography/mass spectrometry. 相似文献
11.
The procedure for calculating linear temperature programmed indices as described in part 1 has been evaluated using five different nonpolar columns, with OV-1 as the stationary phase. For fourty-three different solutes covering five different classes of components, including n-alkanes and alkyl-aromatic compounds, both isothermal and temperature programmed indices were determined. The isothermal information was used to calculate temperature programmed indices. For several linear programmed conditions accuracies better than 0.51T-units were usually obtained. The results are compared with published procedures. It is demonstrated that isothermal retention information obtained on one column can be transferred to another column with the same stationary phase but different column dimensions and/or phase ratio. The temperature programmed indices calculated in this way also have an accuracy better than 0.51T-u. The temperature accuracy and precision of the GC-instrumentation used was of the order of 0.1°C. All calculations can be run with a Basic-programmed microcomputer. 相似文献
12.
Retention indices of methylbenzenes and chlorobenzenes on two fused silica capillary columns, HP-5 (diphenylsiloxane 5% diphenyldimethylsiloxane) and ZB-WAX (polyethylene glycol), have been calculated at various isothermal temperatures and compared with literature data. The retention index temperature effect was studied for each solute, finding greater retention index the higher the column temperature. A comparison between the straight line fit and the fit to the recently proposed equation I = A + B/T +C ln T was carried out. The effect of the stationary phase polarity on the retention index was checked. In general, a greater retention index was found for the more polar stationary phase. The retention indices of the chlorobenzenes are greater than the retention indices of the methylbenzenes, irrespective of the stationary phase and the column temperature. In addition, the influence of the methyl/chlorine substitution on the benzene molecule was investigated at each temperature. The retention indices increased as the number of substituents (methyl/chlorine) increased. The retention index increments of methyl and chloro derivatives are also discussed, which permits to compare the effect of both, methyl or chlorine, chemical functions, for a fixed substituent number in the benzene molecule. 相似文献
13.
GUAN Ya-Feng ZHOU Liang-Mo LI Ling-EDalian Institute of chemical physics Chinese Academy of Sciences P.O.Box Dalian Liaoning China 《中国化学》1994,12(3):265-269
A live retention database for compound identification in isothermal and any step temperature programmed capillary gas chromatography has been developed. The database utilizes the Kovats retention indices of compounds on a given stationary phase and the retention time of n-alkanes measured at isothermal conditions on the column to be used, together with the programming parameters. Identification is performed by search operation that compares the calculated results with the retention values of unknown peaks. Cross-reference of the search results of different operating conditions is performed automatically by the database in order to increase the reliability of the identification. The error of the database conversion is ≤± 0.5 index unit, or ≤± 1% on retention time. This paper describes the principle and the structure of the database in detail. The experimental results for different calsses of compounds tested at divers operating conditions will be presented in Part Ⅱ. 相似文献
14.
Pietrogrande MC Tellini I Pasti L Dondi F Szopa C Sternberg R Vidal-Madjar C 《Journal of chromatography. A》2003,1002(1-2):179-192
A chemometric approach, based on the study of the autocovariance function, is described to study isothermal GC chromatograms of multicomponent mixtures: isothermal GC analysis is the method of choice in space missions since it is, to date, the only method compatible with flight constraints. Isothermal GC chromatograms look inhomogeneous and disordered with peak density decreasing at higher retention times: a time axis transformation is proposed to make retention an homogeneous process so that CH2 addition in terms of an homologous series yields a constant retention increment. The time axis is transformed into a new scale based on the retention times of n-alkanes, as they are the basis of the universal Kovats indices procedure. The order introduced into the chromatogram by retention time linearization can be simply singled out by the experimental autocorrelation function (EACF) plot: if constant inter-distances are repeated in different regions of the chromatogram, well-shaped peaks are evident in the EACF plot. By comparison, with a standard mixture it is possible to identify peaks diagnostic of specific molecular structures: study of the EACF plot provides information on sample chemical composition. The procedure was applied to standard mixtures containing compounds representative of the planetary atmospheres that will be investigated in the near future: in particular, those related to Titan's atmosphere (Cassini-Huygens mission) and cometary's nucleus (Rosetta mission). The employed experimental conditions simulated those applied to GC instruments installed on space probes and landers in space missions. The method was applied to two specific investigations related to space research, i.e., a comparison of retention selectivity of different GC columns and identification of the chemical composition of an unknown mixture. 相似文献
15.
Temperature-programmed retention indices for eleven cyclic organic peroxides were determined by gas chromatography on slightly
polar 5% biphenyl 95% dimethylpolysiloxane columns (DB-5 and Rtx-5MS) at three heating rates (5, 10, and 20° min−1) from 60 to 300°C, using different chromatographs. Cyclic organic diperoxides and triperoxides had nearly constant retention
indices when different heating rates and a short isothermal hold time (5 min) before the programmed increase in temperature
were used. The usefulness of temperature-programmed retention indices was shown by using the data to predict the retention
times and structures of unknown diperoxides or triperoxides derived from ketones. This is the first step in the identification
of unknown cyclic organic peroxides, a process would otherwise require the availability of reference compounds.
Revised: 7 and 17 November 2005 相似文献
16.
Rimma V. Golovnya Lyuba A. Syomina Alexei L. Samusenko 《Journal of separation science》1997,20(11):611-614
It has been demonstated that the considaerable difference in temperature increments of sorption parameters of n-alkanes under isothermal conditions is the main reason for nonlinear dependence of sorption parameters on molecular mass of homolog in temperaturre programmed gas chromatography (TPGC). A new nonlinear 4th parameter equation has been given for calculation of the retention indices. Coefficients of the equation are calculated from n-alkanes. The equation allows extrapolation and interpolation calculations of retention indices under TPGC conditions with experimental precision. The results obtained; for fatty acidkl methyl esters demonstrate the advantage of ovr equation in comparison with van den Dool and Kratz's equation. 相似文献
17.
A systematic approach was utilized to deduce the relation between the programmed and isothermal retention indices in chromatography. The relation was given in the form of a chart from which the equivalent isothermal temperature Te is plotted vs ΔT′ with IP as the parameter. ΔT′ is a function of both the inlet and outlet temperatures during the temperature programmed run and Te is the temperature at which the isothermal index is equal to the programmed index IP. 相似文献
18.
Summary The influence of the isothermal temperature, program rate, initial temperature and flow rate on retention indices was studied. The methods of Kováts, Van Den Dool and Local Lagrange Interpolation are compared. Ten experimental measurements were carried out on a capillary column coated with OV-101 stationary phase. 相似文献
19.
Zhao Y Zhang J Wang B Kim SH Fang A Bogdanov B Zhou Z McClain C Zhang X 《Journal of chromatography. A》2011,1218(18):2577-2583
A method was developed to calculate the second dimension retention index of comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC×GC/TOF-MS) data using n-alkanes as reference compounds. The retention times of the C(7)-C(31) alkanes acquired during 24 isothermal experiments cover the 0-6s retention time area in the second dimension retention time space, which makes it possible to calculate the retention indices of target compounds from the corresponding retention time values without the extension of the retention space of the reference compounds. An empirical function was proposed to show the relationship among the second dimension retention time, the temperature of the second dimension column, and the carbon number of the n-alkanes. The proposed function is able to extend the second dimension retention time beyond the reference n-alkanes by increasing the carbon number. The extension of carbon numbers in reference n-alkanes up to two more carbon atoms introduces <10 retention index units (iu) of deviation. The effectiveness of using the proposed method was demonstrated by analyzing a mixture of compound standards in temperature programmed experiments using 6 different initial column temperatures. The standard deviation of the calculated retention index values of the compound standards fluctuated from 1 to 12 iu with a mean standard deviation of 5 iu. 相似文献
20.
Linear-elution strength theory and temperature-programmed gas chromatography is evaluated as a rapid method for predicting isothermal retention factors and column selectivity. Retention times for a wide range of compounds are determined at the program rates of 3 and 12 °C/min for the temperature range 60 to 160 °C on three open-tubular columns (DB-1701, DB-210 and EC-Wax) and used to predict isothermal retention factors for each column over the temperature range 60 to 140 °C. The temperature-program predicted isothermal retention factors are compared with experimental values using linear regression and the solvation parameter model. It is shown that isothermal retention factors predicted by the linear-elution-strength model only approximately represents the experimental data. The model fails to predict the slight curvature that exists in most plots of the experimental retention factor (log k) as a function of temperature. In addition, regression of the temperature-program predicted isothermal retention factors against the experimental values indicates that the slopes and intercepts deviate significantly from their target values of one and zero, respectively, in a manner which is temperature dependent. The temperature-program predicted isothermal retention factors result in system constants for the solvation parameter model that are different to those obtained from the experimental retention factors. These results are interpreted as indicating that linear-elution-strength theory predicts retention factors that fail to accurately model stationary phase interactions over a wide temperature range. It is concluded that temperature-program methods using linear-elution-strength theory are unsuitable for constructing system maps for isothermal separations. 相似文献