不锈钢支撑双层类脂膜离子传感器的电化学研究与应用

C60;分子器件;不锈钢支撑双层类脂膜离子传感器的电化学研究与应用     

双层类脂膜及其在电化学生物传感器中的应用

详细评述了各种双层类脂膜包括传统的双层类脂膜（ＢＬＭ）、固体载体支撑的自组双层类脂膜（ｓ－ＢＬＭ）、固体载体支撑的混合双层类脂膜（ｅ－ＢＬＭ）的制备方法和特性,比较了其优缺点。介绍了双层类脂膜在电化学生物传感器中的应用,并展望了发展前景。     

5－（4－乙酰氧基苯基），10，15，20－三苯基卟啉金属配合物修饰的铂支撑双层脂膜的IV性质

合成并表征了５－（４－乙酰氧基苯基），１０，１５，２０－三苯基卟啉及其金属配合物，将它们作为修饰物，制得铂支撑的双层脂膜，用循环伏安法研究了抗坏血酸的氧化还原反应，并对其机理作了探讨。     

细胞色素C551在ITO电极上的直接电化学

在不加任何电子转移促进剂的条件下,用循环伏安法(CV)和微分脉冲伏安法(DPV)观察到细胞色素C551在ITO导电玻璃上的直接电化学行为.结果显示电极反应过程为准可逆性质,计算得到细胞色素C551的扩散系数、式电位和异相电子转移标准速率常数,并对其在ITO导电玻璃电极上的电子转移机制进行了初步分析.     

细胞色素C在离子液体修饰电极上的电化学行为

分别合成了疏水和亲水性咪唑类离子液体,并制备了相应的两种离子液体修饰的玻碳电极。循环伏安法测量结果显示,细胞色素C在离子液体修饰的玻碳电极上的电子传递过程为一扩散控制的准可逆反应,表明咪唑类离子液体也可用作细胞色素C电子传递的有效促进剂。电化学交流阻抗谱的测量结果得到了与循环伏安相同的结论。     

双层类脂膜成膜过程的电化学方法研究

应用循环伏安法(CV),计时电流法(I＿T)和开路电压法(V＿T)研究了导电玻璃支撑的双层类脂膜(ITO/s＿BLM)的成膜动力学过程.实验表明,导电玻璃(ITO)是一种理想的成膜支撑基底,ITO/S＿BLM膜具有成膜快稳定时间长的特点.与循环伏安法相比,计时电路法和开路电路法能更为详细地给出膜系统组装的瞬时信息.ITO/s＿BLM体系的膜电压实验值约为0.1V.     

细胞色素C的薄层光谱电化学研究

细胞色素C(Cyt. C)在电极界面上的电子传递十分缓慢,只有在适当的电子迁移中介体(Mediator)或促进剂(Promoter)参与下才能以较快的速度进行反应,本文报道了以4,4′-二硫基联吡啶(PySSPy)作电极反应促进剂,用薄层光谱电化学技术研究细胞色素C在金微网栅薄层透光电极界面上的电化学过程,测定了电极反应的热力学参数E~(o′)及n,并与循环伏     

细胞色素C在单糖修饰金电极上的直接电化学

Hill等发现在4,4′-联吡啶存在时,细胞色素C在金电极上能进行准可逆的电化学反应。在研究细胞色素C的直接电化学过程中,人们又发现一些生物小分子如氨基酸、嘌呤等对细胞色素C的电化学反应有促进作用,但迄今未见有关糖类分子对细胞色素C电化学反应促进作用研究的报道。本文研究了5种单糖对细胞色素C电化学反应的促进作用。     

基于纳米结构双层类脂膜的雌激素传感器

采用铂盘电极支撑的纳米金修饰的双层类脂膜(Pt/BLM/Au)来固定雌激素受体(ER),制备Pt/BLM/Au/ER用于检测雌激素的传感器.电极不同修饰状态分别用循环伏安法和交流阻抗法进行表征.以交流阻抗法测得电极结合雌激素后阻抗的改变与17β-雌二醇(E2)的浓度在5～150 ng/L范围呈良好线性关系,相关系数r=...     

槲皮素修饰的双层脂膜的伏安研究

槲皮素修饰的从层脂膜作为研究抗病毒药物的一种模型体系,用循环伏安法测定了槲皮素在脂膜界面上的氧化还原性质和配位性质。在电位扫描过程中,微量铜离子能催化槲皮素的氧化,当双层脂膜的两侧存在着合适的氧化还原偶时,就会产生跨膜的电子传递。     

Single-channel recordings of gramicidin at agarose-supported bilayer lipid membranes formed by the tip-dip and painting methods.

Agarose-supported BLMs were prepared by the tip-dip and painting methods, and single-channel recordings of gramicidin were examined for the development of an ion-channel sensor. The supported BLMs formed by the tip-dip method had an electric resistance of >1.0 x 10(11) omega and a longer lifetime as compared with unsupported ones, which enabled single-channel recordings of gramicidin. The supported BLMs formed by the painting method also enabled single-channel recordings, but the lifetime was shorter than that of unsupported planar BLMs formed by the monolayer folding method.     

Self-assembled BLMs: biomembrane models and biosensor applications

In the last few years, there have been a number of research papers on self-assemblies of molecules as ‘advanced’ or ‘smart’ materials. The inspiration for this exciting research, without question, comes from the biological world, where, for example, the lipid bilayer of the cell membrane is the most important self-assembling system. Although the first report on self-assembled bilayer lipid membranes (BLMs) in vitro was published in 1962, interface science, including surface and colloid science, has been dealing with these interfacial self-assemblies of amphiphilic molecules since Robert Hooke's time (1672). BLMs have been used in a number of applications, ranging from basic membrane biophysics studies to the conversion of solar energy via water photolysis, and to biosensor development using supported bilayer lipid membranes (s-BLMs and sb-BLMs). This paper briefly summarizes the past research on the use of BLMs as models of biological membranes and describes some details of our current work on supported BLMs as practical biosensors. Additionally, experiments carried out in close collaboration with others on s-BLMs and sb-BLMs are presented.     

Biophysical aspects of agar-gel supported bilayer lipid nembranes: a new method for forming and studying planar bilayer lipid membranes

Conventional bilayer lipid membranes (BLMs), formed by either the painting method or the Langmuir-Blodgett technique, lack the desired stability. This paper presents a simple method for forming long-lived BLMs on agar-gel supports. The supported BLM reported has a greatly improved mechanical stability and also has desirable dynamic properties. These self-assembled BLMs are of significant interest, in view of their similarity of biological membranes, their molecular dimension and their spontaneous formation.     

The lipid bilayer concept and its experimental realization: from soap bubbles,kitchen sink,to bilayer lipid membranes

The inspiration for lipid bilayer research, without question, comes from the biological world. Although self-assembled bilayer lipid membranes (BLMs) in vitro, were first reported in 1961, experimental scientists have been dealing with BLM-type interfacial adsorption phenomena since Robert Hooke’s time (1672). BLMs (of planar lipid bilayers) have been used in a number of applications ranging from basic membrane biophysics including transport, practical AIDS research, and ‘microchips’ studies, to the conversion of solar energy via water photolysis, to biosensor development using supported bilayer lipid membranes (s-BLMs), and to photobiology comprising apoptosis and photodynamic therapy. This paper presents an overview of the origin of the lipid bilayer concept and its experimental realization, as well as the studies of our laboratory and recent research of others on the use of BLMs as models of certain biomembranes. In addition, we describe briefly our present work on supported BLMs as biosensors and molecular devices; the experiments carried out in close collaboration with colleagues on s-BLMs are delineated.     

The 40th anniversary of bilayer lipid membrane research

This paper presents a historic perspective on the origin of the lipid bilayer concept and its experimental realization. Additionally, current studies in close collaboration with our colleagues on the use of supported BLMs as biosensors and molecular devices are delineated. Further, recent research of others on BLMs (planar lipid bilayers) is referenced.     

Molecular simulations suggest protein salt bridges are uniquely suited to life at high temperatures

A series of explicit solvent molecular dynamics simulations has been performed to investigate the temperature dependence of salt bridge interactions between two freely diffusing amino acids. The simulations, performed at 25, 50, 75, and 100 degrees C, allow a large number of distinct association and dissociation events to be directly observed, without the imposition of additional forces to drive association. Analysis of contact frequencies for atom pairs demonstrates that the number of salt bridge contacts between the two molecules is unaffected by temperature, whereas the numbers of hydrophobic and polar contacts are greatly diminished. A second, independent set of simulations-using rigid, prototypical molecule types-allows the differing temperature dependences of hydrophobic, polar, and salt bridge interactions to be unambiguously examined. In the prototype molecule simulations, the salt bridge interaction is found to substantially increase in stability at 100 degrees C relative to 25 degrees C. This difference in behavior between flexible amino acids and rigid prototype molecules is perhaps a direct manifestation of the effects of conformational entropy on association thermodynamics. Overall, the results demonstrate that salt bridge interactions are extremely resilient to temperature increases and, as such, are uniquely suited to promoting protein stability at high temperatures.     

细胞色素C在4,5-二氮芴-9-酮修饰玻碳电极上的直接电化学研究

采用循环伏安法探讨了细胞色素C(Cyt C)在4, 5-二氮芴-9-酮(dafo)修饰玻碳电极表面的电化学行为.结果表明,Cyt C在dafo修饰电极上呈现一对峰形较好且准可逆的氧化还原峰,其式电位(E0′)为13 mV,峰电流与扫描速度呈线性关系,该电极过程是表面控制过程,电化学反应效率常数(ks)为0.89/s.固定在dafo上的Cyt C能促进H2O2的催化还原,响应快速而灵敏(<10 s),峰电流与H2O2浓度在5.0×10-6～3.0×10-4 mol/L范围内呈线性关系;检出限2.5×10-6 mol/L;米氏常数为1.07 mmol/L,显示出较好的亲和力.     

Supramolecular polymer gels from polystyrene bearing cyclic amidine Group and acrylic acid/n‐butyl acrylate copolymers

We have prepared supramolecular polymer gels by mixing solutions of a polystyrene bearing cyclic amidine pendant groups (Poly‐A) and copolymers of acrylic acid and n‐butyl acrylate (Poly‐C), followed by evaporation. FT‐IR analysis indicated that the gels were formed through three‐dimensional network of the amidinium‐carboxylate salt bridge. DSC study showed that the Poly‐A and the Poly‐C were miscible when the salt bridge content was high. On the other hand, the mixtures with small salt bridge content showed phase separation. Dynamic shear measurements showed that the gel prepared from Poly‐A and Poly‐C with acrylic acid unit content of 15% had G' higher than G″ over a temperature range of ?22 °C to 32 °C, in which the G' value reached almost 1 MPa. The gel had a crossover point of G' and G″ at 32 °C, very close to room temperature, which suggested facile processability. © 2015 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2016 , 54, 765–770