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1.
Thermal analysis of soil amended with sewage sludge and biochar from sewage sludge pyrolysis 总被引:1,自引:0,他引:1
The main objective of the present study is to study the behaviour of sewage sludge and biochar from sewage sludge pyrolysis
after addition to soil in a context of a temperate agricultural soil. For this, an incubation experiment was designed during
200 days. Carbon mineralization of soil amended with sewage sludge and biochar at two different rates (4 and 8 wt%) was evaluated.
Differential thermal analysis, thermogravimetry and the first derivate of the TG were performed in oxidizing conditions on
soil samples before and after incubation. Biochar obtained from sewage sludge pyrolysis at 500 °C was more stable in soil
than original sewage sludge. After incubation experiment, the reduction of soil organic matter content was significantly lower
in soil amended with biochar than in soil amended with sewage sludge. The thermostability index WL3/WL2 decreases after incubation in soil amended with biochar, however it increases in case of soil treated with sewage sludge. 相似文献
2.
Ana Méndez Sandra Barriga Antonio Saa Gabriel Gascó 《Journal of Thermal Analysis and Calorimetry》2010,99(3):993-998
The objective of this work is to study the removal of malachite green (MG) from water by adsorbents obtained from pyrolysis
of two paper industry waste materials: one de-inking paper sludge (HP) and one organic sludge from virgin pulp mill (RT).
Both adsorbents showed elevated MG removal. Maximum adsorption Q
0 obtained by Langmuir equation was higher for the adsorbent from HP (HP-3, 982 mg/g) than RT (RT-3, 435 mg/g). However, K
L (Langmuir) and 1/n (Freundlich) indicated that affinity and intensity of adsorption is higher for the adsorbents from RT. Thermal analysis (TG,
DTG and DTA) of adsorbents before and after MG removal was performed in N2 atmosphere. 相似文献
3.
Emo Chiellini Andrea Corti Salvatore D'Antone Roberto Solaro 《Macromolecular Symposia》1999,144(1):127-139
Investigation of the biodegradability of water soluble poly(vinyl alcohol) (PVA) based blown films was carried out under different lab-scale environmental conditions. In particular respirometric tests were utilized in order to evaluate the biodegradability of PVA films in composting, in modified Sturm test and in soil burial simulation tests. Several microbial inocula present in river water, mature compost, forest and farm soils as well as sewage sludge from municipal and paper mill wastewater treatments plants were utilized for the relevant tests. A mixed PVA-degrading microbial culture was obtained by a common enrichment procedure by using sewage sludge from paper mill as inoculum; this culture was tentatively utilized for the isolation of single PVA-degrading microorganisms. As a first result we can stress that significant biodegradation extent in fairly low incubation time can be obtained only in the presence of acclimated microbial populations such as those deriving from paper mill sewage sludge, in liquid cultures. Nevertheless separation of single degrading microbial species was impossible most likely due to the establishment of symbiotic or commensal interactions between the single components of the PVA-degrading mixed cultures. On the other hand, limited mineralization rates were recorded in solid cultures in the presence of soil or compost. Finally, a mechanism of degradation of polymer chains unlike random or unzipping was suggested in the presence of either PVA-degrading mixed culture and its filtrate by means of viscometric determinations of molecular weight within the time. 相似文献
4.
Zusammenfassung Phosphinboran und Phosphoniumjodid reagieren mit NaBH4 unter H2-Entwicklung zu H2P(BH3)2–Na+(I), Phenylphosphinboran zuPhHP(BH3)2–Na+ (II). Methylphosphinboran, Phenylphosphin und die Anionen von I und II reagieren nicht mit NaBH4, da die Acidität des an Phosphor gebundenen Wasserstoffs zu gering ist. Auch (PhHP·BCl2)3 reagiert mit NaBH4 unter Wasserstoffentwicklung.
Reaction of phosphine borane, phenylphosphine borane and phosphonium iodide with sodium tetrahydridoborate
The reaction of phosphine borane and phosphonium iodide with NaBH4 yields H2P(BH3)2–Na+ (I), of phenylphosphine boranePhHP(BH3)2–Na+ (II) hydrogen being evolved in both reactions. Methylphosphine borane, phenylphosphine and the anions of I and II do not react with NaBH4 on account of the reduced acidity of the hydrogen atoms bound to phosphorus. Likewise hydrogen is evolved if (PhHP·BCl2)3 reacts with NaBH4.相似文献
5.
Chairattanamanokorn P Tapananont S Detjaroen S Sangkhatim J Anurakpongsatorn P Sirirote P 《Applied biochemistry and biotechnology》2012,166(2):389-401
Dark anaerobic fermentation is an interesting alternative method for producing biohydrogen (H2) as a renewable fuel because of its low cost and various usable organic substrates. Pulping sludge from wastewater treatment
containing plentiful cellulosic substrate could be feasibly utilized for H2 production by dark fermentation. The objective of this study was to investigate the optimal proportion of pulping sludge
to paper waste, the optimal initial pH, and the optimal ratio of carbon and nitrogen (C/N) for H2 production by anaerobic seed sludge pretreated with heat. The pulping sludge was pretreated with NaOH solution at high temperature
and further hydrolyzed with crude cellulase. Pretreatment of the pulping sludge with 3% NaOH solution under autoclave at 121 °C
for 2 h, hydrolysis with 5 FPU crude cellulase at 50 °C, and pH 4.8 for 24 h provided the highest reducing sugar production
yield (229.68 ± 2.09 mg/gTVS). An initial pH of 6 and a C/N ratio of 40 were optimal conditions for H2 production. Moreover, the supplement of paper waste in the pulping sludge enhanced the cumulative H2 production yield. The continuous hydrogen production was further conducted in a glass reactor with nylon pieces as supporting
media and the maximum hydrogen production yield was 151.70 ml/gTVS. 相似文献
6.
Kotseruba V. A. Reut S. I. Chikhichin D. G. Kamalov G. L. 《Theoretical and Experimental Chemistry》2001,37(1):28-35
Results are given for a kinetic study of the decomposition of the hydroperoxide (HP) of dibenzyl ether (DBE) to give oxidates of the latter in the presence of complexes Cr2MOPiv6·3L(M = Cr(III), Fe(III), Ni(II), and Co(II); L = PPy, -Pic, and H2O). The experimental data were satisfactorily described assuming that the formation of an HP–catalyst intermediate complexes is the rate-limiting step and the catalyst is deactivated by components of the DBE oxidate and taking account of the noncatalytic decomposition of the HP. 相似文献
7.
Model chemistry G3(MP2,CC)//B3LYP/6-311G(d,p) calculations of the potential energy surface for the reaction of phenyl radical (C6H5) with phenylacetylene (C8H6) have been carried out and combined with Rice-Ramsperger-Kassel-Marcus/Master Equation calculations of temperature- and pressure-dependent rate constants. The results showed that the reaction can serve as a viable source for the formation of phenanthrene via an indirect route involving a primary reaction of phenyl addition to the ortho carbon in the ring of phenylacetylene and H elimination producing 2-ethynylbiphenyl followed by secondary H-assisted isomerization of 2-ethynylbiphenyl to phenanthrene. In the secondary reaction, the H atom adds to the α carbon of the ethynyl side chain, then a six-member ring closure takes place followed by aromatization via an H loss. The channel of H addition to the side chain of 2-ethynylbiphenyl appears to be much faster than H addition to the ortho carbon in the ethynyl-substituted ring leading back to the initial C6H5 + C8H6 reactants. Rate constants for the primary C6H5 + C8H62-ethynylbiphenyl ( p1 ) + H and secondary p1 + Hphenanthrene ( p2 ) + H reactions have been computed in the temperature range of 500-2500 K at pressures of 30 Torr, 1, 10, and 100 atm and fitted to modified Arrhenius expressions. The suggested kinetic scheme and rate constants are proposed as a prototype for the modeling of the growth of polycyclic aromatic hydrocarbons via the phenyl addition-dehydrocyclization (PAC) mechanism involving an addition of a PAH radical to an ethynyl-substituted PAH molecule. 相似文献
8.
A new method based on probe sonication extraction (USP) prior to high performance liquid chromatography (HPLC) has been developed for the determination of linear alkylbenzene sulfonates (LAS) from sewage sludge. The optimized method was designed to be cost effective compared to existing extraction methods (ultrasonic assisted extraction, Soxhlet or pressurized liquid extraction) which may require large quantities of organic solvents, or costly instrumentation or equipment.The main factors affecting the extraction efficiency (extractant volume, ultrasounds power and extraction time) were optimized using compost sludge. The detection limit of total LAS in the sludge was 10 mg kg− 1. The extraction of C10-C13 homologues is carried out using an extraction time of 7 min with 10 mL of methanol. Liquid chromatography with fluorescence (FL) detector is used for determination of LAS homologues. A mobile phase acetonitrile-water containing 0.1 M NaClO4 (65:35) and isocratic elution was used. Compounds were eluted over 6 min at a flow rate of 1 mL/min. Polar interferences are eluted between 0 and 2 min and no purification of the samples is required prior to the final determination by high performance liquid chromatography (HPLC). The recoveries of LAS in spiked sewage sludge were between 84.0% and 97.0%, which reflect the efficiency of the method for extraction of these analytes from sewage sludge. Concentration levels found were between 11,858 mg kg− 1 for digested sludge and 2379 mg kg− 1 for compost sludge. 相似文献
9.
Mukesh Goel Jean-Marc Chovelon Corinne Ferronato Remy Bayard T.R. Sreekrishnan 《Journal of photochemistry and photobiology. B, Biology》2010,98(1):1-6
In this work, the performance of integrated photocatalytic and biological treatment was studied for the degradation of 4-chlorophenol (MCP) present in wastewaters. Photocatalysis was used as a pre-treatment to biological degradation. Pollutant removal efficiency was quantified using MCP removal and total organic carbon (TOC) removal. Both photocatalytic as well as biological treatments were carried out in batch reactors, using TiO2 as the photocatalyst. The inoculum for biological experiments was obtained from paper mill effluent treatment plant and was developed through a process of selection and acclimatization. Effect of TiO2 concentration on the photocatalytic degradation of MCP was studied along with the effect of the duration of photochemical oxidation and glucose concentrations (0 g/L, 1 g/L and 2 g/L) on the biodegradation of MCP. Integrated biological and photochemical degradation was found to be more effective in treating MCP, especially at higher concentrations (400 mg/L). An initial MCP concentration of 400 mg/L required 96 h for complete mineralization when treated with the process combination, whereas the treatment went on up to 264 h when biodegradation alone was employed. 相似文献
10.
Plastics are a major constituent of municipal solid waste that pose a growing disposal and environmental pollution problem due to their recalcitrant nature. To reduce their environmental impacts and allow them to be transformed during organic waste recycling processes, various materials have recently been introduced to improve the biodegradability of plastics. These include conventional plastics amended with additives that are meant to enhance their biodegradability, bio-based plastics and natural fiber composites. In this study, the rate and extent of mineralization of a wide range of commercially available plastic alternative materials were determined during composting, anaerobic digestion and soil incubation. The biodegradability was assessed by measuring the amount of carbon mineralized from these materials during incubation under conditions that simulate these three environments and by examination of the materials by scanning electron micrography (SEM). The results showed that during a 660 day soil incubation, substantial mineralization was observed for polyhydroxyalkanoate plastics, starch-based plastics and for materials made from compost. However, only a polyhydroxyalkanoate-based plastic biodegraded at a rate similar to the positive control (cellulose). No significant degradation was observed for polyethylene or polypropylene plastics or the same plastics amended with commercial additives meant to confer biodegradability. During anaerobic digestion for 50 days, 20–25% of the bio-based materials but less than 2% of the additive containing plastics were converted to biogas (CH4 + CO2). After 115 days of composting, 0.6% of an additive amended polypropylene, 50% of a plastarch material and 12% of a soy wax permeated paper pulp was converted to carbon dioxide. SEM analysis showed substantial disintegration of polyhydroxyalkanoate-based plastic, some surface changes for other bio-based plastics and coconut coir materials but no evidence of degradation of polypropylene or polypropylene containing additives. Although certain bio-based plastics and natural fibers biodegraded to an appreciable extent in the three environments, only a polyhydroxyalkanoate-based resin biodegraded to significant extents during the time scale of composting and anaerobic digestion processes used for solid waste management. 相似文献
11.
A pilot-scale microwave heating apparatus was constructed for the production of bio-oil from sewage sludge, and the effects of important microwave processing parameters and chemical additives on the quality and yield of bio-oils were investigated. It was found that bio-oil was mainly formed at the pyrolysis temperature range of 200–400 °C. A higher heating rate (faster pyrolysis) not only increased the yield of bio-oil, but also improved the quality of bio-oil according to the elemental composition and calorific values. The maximum bio-oil yield was 30.4% of organic fraction, obtained from the pyrolysis of original sewage sludge at microwave radiation power of 8.8 kW and final pyrolysis temperature of 500 °C. All of five simple additives (KOH, H2SO4, H3BO3, ZnCl2, and FeSO4) reduced the bio-oil yield, but the composition and property of bio-oil varied with the additive types greatly. KOH, H2SO4, H3BO3 and FeSO4 were found to improve the quality of bio-oils remarkably according to the calorific value, density, viscosity and carbon content of bio-oils, but ZnCl2 treatment went against that. GC–MS analysis of the bio-oils showed that, alkali treatment promoted the formation of alkanes and monoaromatics, while acid treatment favored the formation of heterocyclics, ketones, alcohols and nitriles. Compared with sulfate slat FeSO4, chloride salt ZnCl2 was a better catalyst for selective catalytic pyrolysis of sewage sludge. The addition of ZnCl2 only promoted the formation reactions of a few kinds of nitriles and ketones remarkably. It is technologically feasible to produce bio-oil form microwave-induced pyrolysis of sewage sludge by optimizing pyrolysis conditions and selecting appropriate additives. 相似文献
12.
Chengfeng Li Ning Hua Chenyun Wang Xueya Kang Tuerdi Wumair Ying Han 《Journal of Solid State Electrochemistry》2011,15(9):1971-1976
LiFePO4/C composite cathode material is prepared by ball milling with the assistance of EDTA chelation with using water as the media
of ball mill procedure. FePO4 and LiOH are used as starting materials; a certain amount of glucose is used as carbon sources and reduction agent. The structure
and morphology of the composite are characterized by X-ray diffraction and scanning electron microscopy. Cyclic voltammetry,
AC impedance measurements, and galvanostatic charge–discharge and cycling performances are used to characterize its electrochemical
properties. The results indicate that the performances of composites prepared by chelation-assisted method are much better
than common ball milling method which using alcohol or acetone as the media of ball mill procedure. The stable discharge capacity
of the prepared composite is 150 and 105 mAh g−1 at 1 and 10 C rate, respectively. 相似文献
13.
Paula Guerra Ethel Eljarrat Damià Barceló 《Analytical and bioanalytical chemistry》2010,397(7):2817-2824
This paper describes the development of a methodology for the simultaneous determination and quantification of hexabromocyclododecane
(HBCD), tetrabromobisphenol A (TBBPA), and related compounds (bisphenol A, monobromobisphenol A, dibromobisphenol A, and tribromobisphenol
A) in sludge and sediment samples. The selected method is based on an extraction with dichloromethane: methanol followed by
purification via SPE C18 cartridges. Instrumental determination was carried out by liquid chromatography–quadrupole linear ion trap mass spectrometry
(LC-QqLIT-MS), with quantification based on isotopic dilution method. Analyte recoveries were in the range of 39–120% and
88–126% for spiked sewage and sediment, respectively. Repeatability of replicate extractions was better than 13% relative
standard deviation. Linearity was checked in the range of 0.05 and 25 injected nanograms. Limits of detection (LODs) and limits
of quantification (LOQs) were in the range of 0.6 and 2.7 ng/g and 1.4 and 66 ng/g for sediment and sludge samples, respectively.
The developed method was applied to sewage sludge and sediment samples collected along the Ebro River and Cinca River, one
of its tributaries (northeast of Spain). TBBPA levels in sewage sludge ranged from not quantified to 1,329 ng/g dw, whereas
levels in sediment samples were lower, between not detected and 15 ng/g dw. As regards HBCD, concentrations were between not
detected and 375 ng/g for sludge samples and 0.8 and 1850 ng/g for sediments. 相似文献
14.
The pretreatment of waste-activated sludge (WAS) by electron beam irradiation was studied in order to improve anaerobic sludge
digestion. The irradiation dose of the electron beam was varied from 0.5 to 10 kGy. Batch and continuous-flow stirred tank
reactors (CFSTRs) were operated to evaluate the effect of the electron beam pretreatment on anaerobic sludge digestion. Approximately
30–52% of the total chemical oxygen demand (COD) content of the WAS was solubilized within 24 h after electron beam irradiation.
A large quantity of soluble COD, protein, and carbohydrates leached out from cell ruptures caused by the electron beam irradiation.
Volatile fatty acids production from the irradiated sludge was approx 90% higher than that of the unirradiated sludge. The
degradation of irradiated sewage sludge was described by two distinct first-order decay rates (k
1 and k
2). Most initial decay reaction accelerated within 10 d, with an average k
1 of 0.06/d for sewage sludge irradiated at all dosages. The mean values for the long-term batch first-order decay coefficient
(k
2) were 0.025/d for irradiated sewage sludge and 0.007/d for unirradiated sludge. Volatile solids removal efficiency of the
control reactor fed with unirradiated sewage sludge at a hydraulic retention time (HRT) of 20 d was almost the same as that
of the CFSTRs fed with irradiated sludge at an HRT of 10 d. Therefore, disintegration of sewage sludge cells using electron
beam pretreatment could reduce the reactor solid retention time by half. 相似文献
15.
A. Mndez J.M. Fidalgo F. Guerrero G. Gasc 《Journal of Analytical and Applied Pyrolysis》2009,86(1):66-73
Paper industry generates a considerable amount of wastes. Their composition mainly depends on the type of paper produced and the origin of cellulose fibres. Nowadays, in Spain, 40% of solid wastes generated by the paper and pulp industry are deposited directly in landfill, 25% are used in the agriculture, 13% in the ceramic industry and 7% in the concrete production. In the last years, thermal treatment methods like combustion, pyrolysis and gasification have been widely study as alternative techniques for the valorization of different organic waste materials. The main objective of the present work is to study the pyrolysis behaviour of different paper mill waste materials. For this reason, a wide characterization of eight paper mill waste materials from different origins was performed using SEM, FTIR, DRX and thermogravimetric techniques. Paper mill sludges from recycled paper, mainly wastes obtained from deinking process, showed high CaCO3 and clays contents. Compared with the elevated total organic matter content (TOM) of paper mill waste materials their low organic carbon content determined by Cr2O72− oxidation reveals the elevated chemical stability of organic matter, due to high content on cellulose fibres. Analysis of samples by SEM indicates that successive recycled processes of paper leads to paper mill waste materials with more degraded fibres. XRD analyses show as crystalline cellulose was present in reject and primary sludge from paper mills that produced paper from virgin wood. However, crystalline cellulose content significantly decreased in waste materials from recycled paper. Finally, thermogravimetric analysis indicates that presence or mineral matter and degradation of cellulose significantly influences their pyrolysis behaviour. In general, weight loss of paper mill waste materials started at lower temperatures than pure cellulose. In waste materials from recycled paper weight loss continues at temperatures highest than 500 °C due to kaolinite dehydration and carbonates decomposition. 相似文献
16.
造纸污泥添加剂对麦秆灰烧结熔融特性的影响 《燃料化学学报》2017,45(11):1323-1331
利用灰熔点测试仪、XRD及XRF等仪器,对比研究了造纸污泥(脱墨污泥、造纸废水污泥)、城市废水污泥作为添加剂对麦秆灰熔融特性的影响,考察了烧结和熔融过程中的组分变化,分析了污泥添加剂对麦秆灰的作用机理;进一步将污泥添加剂与常规添加剂进行灰熔融特性对比研究。研究发现,添加比例控制为3%-10%,造纸污泥(脱墨污泥、造纸废水污泥)软化温度提升效果均优于城市废水污泥;在添加比例控制为5%时,造纸废水污泥对麦秆灰软化温度提升效果最好;增大添加比例过程中,造纸废水污泥Al_2O_3修饰骨架作用明显,但灰中长石类物质逐渐增多使得软化温度提升效果下降;在不同温度下,脱墨污泥主要是通过形成硅铝榴石使得灰熔点提升,造纸废水污泥则主要是通过生成高熔点物质CaSiO_3抑制低熔点硅酸盐形成,城市废水污泥升温中存在明显SiO_2晶态转变过程;使用污泥添加剂作为抗结渣添加剂具有良好应用前景。 相似文献
17.
Tien Thanh Nguyen Huu Hao Ngo Wenshan Guo Jianxin Li Andrzej Listowski 《Applied biochemistry and biotechnology》2012,167(6):1678-1687
The performance of a novel sponge-submerged membrane bioreactor (SSMBR) was evaluated to treat primary treated sewage effluent at three different activated sludge concentrations. Polyurethane sponge cubes with size of 1?×?1?×?1?cm were used as attached growth media in the bioreactor. The results indicated the successful removal of organic carbon and phosphorous with the efficiency higher than 98% at all conditions. Acclimatised sponge MBR showed about 5% better ammonia nitrogen removal at 5 and 10?g/L sludge concentration as compared to the new sponge system. The respiration test revealed that the specific oxygen uptake rate was around 1.0?C3.5?mgO2/gVSS.h and likely more stable at 10?g/L sludge concentration. The sludge volume index of less than 100?mL/g during the operation indicated the good settling property of the sludge. The low mixed liquor suspended solid increase indicated that SSMBR could control the sludge production. This SSMBR was also successful in reducing membrane fouling with significant lower transmembrane pressure (e.g. only 0.5?kPa/day) compared to the conventional MBR system. Further study will be conducted to optimise other operating conditions. 相似文献
18.
This study was conducted to investigate the effects of gamma ray irradiation on the solubilization of waste sewage sludge. The recovery of an organic carbon source from sewage sludge by gamma ray irradiation was also studied. The gamma ray irradiation showed effective sludge solubilization efficiencies. Both soluble chemical oxygen demand (SCOD) and biochemical oxygen demand (BOD5) increased by gamma ray irradiation. The feasibility of the solubilized sludge carbon source for a biological nitrogen removal was also investigated. A modified continuous bioreactor (MLE process) for a denitrification was operated for 20 days by using synthetic wastewater. It can be concluded that the gamma ray irradiation was useful for the solubilization of sludge and the recovery of carbon source from the waste sewage sludge for biological nitrogen removal. 相似文献
19.
Electrosorption of uranium ions on activated carbon fibers 总被引:1,自引:0,他引:1
Chong-Hun Jung Hye-Young Lee Jei-Kwon Moon Hui-Jun Won Yong-Gun Shul 《Journal of Radioanalytical and Nuclear Chemistry》2011,287(3):833-839
A study on the electrosorption of uranium (U(VI)) ions onto a porous activated carbon fiber was performed to treat lagoon
sludge containing 100 mg/L uranium and high concentration of chemical salts composed 3.8% NaNO3, 19.8% NH4NO3, 1.9% Ca(NO3)2. The applied negative potential increased the adsorption kinetics and capacity in comparison to the open-circuit potential
adsorption for uranium ions. When applying potential at −0.9 V (vs. Ag/AgCl) and pH 4, above 99% of the uranium is selectively
removed from the 100 mg/L influent by electrosorption, and the cumulative amount of uranium for 50 h is about 600 mguranium/gACF. The high selectivity of elctrosorption process for uranium was probably caused by the difference of charge density of cations.
More than 99% of adsorbed uranium ions was desorbed at a potential of +1.2 V and pH 3. The electrosorption of uranium onto
the porous activated carbon fiber electrode is due to an ion exchange type reaction between the uranium ions and surface acid
groups on carbon surface. Cyclic electrosorption test consisting of adsorption and desorption step shows that the activated
carbon fiber electrode is easily regenerated in situ, indicating it is a reversible process. 相似文献
20.
Chattopadhyay S Srivastava AK Bisaria VS 《Applied biochemistry and biotechnology》2002,102(1-6):381-393
The root explants of the germinated seedlings of Podophyllum hexandrum were grown in MS medium supplemented with indole acetic acid (IAA) (2 mg/L) and activated charcoal (0.5%), and healthy callus
culture was obtained after incubation for 3 wk at 20°C. The cultivation of plant cells in shake flask was associated with
problems such as clumping of cells and browning of media, which were solved by the addition of pectinase and polyvinylpyrrolidone.
The effect of major media components and carbon source was studied on the growth and podophyllotoxin production in suspension
culture. It was found that glucose was a better carbon source than sucrose and that NH4
+:NO3
− ratio (total nitrogen concentration of 60 mM) and PO4
3− did not have much effect on the growth and product formation. The relative effect of culture parameters (inoculum level,
pH, IAA, glucose, NH4
+:NO3
− ratio, and PO4
3−) on the overall growth and product response of the plant cell suspension culture was further investigated by Plackett-Burman
design. This indicated that inoculum level, glucose, IAA, and pH had significant effects on growth and production of podophyllotoxin.
To identify the exact optimum concentrations of these parameters on culture growth and podophyllotoxin production, central
composite design experiments were formulated. The overall response equations with respect to growth and podophyllotoxin production
as a function of these culture parameters were developed and used to determine the optimum concentrations of these parameters,
which were pH 6.0, 1.25 mg/L of IAA, 72 g/L of glucose, and inoculum level of 8 g/L. 相似文献