共查询到20条相似文献,搜索用时 15 毫秒
1.
Aristotelis B. Syropoulos Evangelos G. Sarantonis Antony C. Calorkerinos 《Analytica chimica acta》1990
The chemiluminescent oxidation of sulphite by bromate was investigated and compared with that by cerium(IV). The reaction is sensitized by various steroid hormones which can thus be determined in the ranges 0.50–20.0μg ml 1 for cortisone; 0.50–5.00 μg ml?1 for hydrocortisone and progesterone and 0.50–6.00 μg ml?1 for testosterone and corticosterone. 相似文献
2.
A simple, highly sensitive chemiluminescent method using flow injection is described for the determination of three substituted benzamides, namely: sulpiride, sultopride and tiapride. The method is based on the sensitizing effect of these drugs on the chemiluminometric oxidation of sulphite by cerium(IV). The different experimental parameters affecting the chemiluminescence intensity were carefully studied and incorporated into the procedure. The method permits the determination of 0.05-2.5 mug ml(-1) sulpiride, 0.01-2.5 mug ml(-1) sultopride hydrochloride and 0.01-1.5 mug ml(-1) tiapride hydrochloride with minimum detectability of 0.01 mug ml(-1). The method was applied to the determination of these benzamides in pharmaceutical preparations and biological fluids. 相似文献
3.
4.
5.
Flow injection analysis of ketoprofen based on the order transform second chemiluminescence reaction
Zhuang Y Cao G Ge C 《Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy》2012,85(1):139-144
This paper explores an order-transform-second-chemiluminescence (OTSCL) method combining the flow injection technique for the determination of ketoprofen. When ketoprofen solution was injected into the mixture after the end of the reaction of alkaline luminol and sodium periodate or sodium periodate solution was injected into the reaction mixture of ketoprofen and alkaline luminol, a new chemiluminescence (CL) reaction was initiated and strong CL signal was detected. A mechanism for the OTSCL has been proposed on the basis of the chemiluminescence kinetic characteristic, UV-visible absorption and chemiluminescent spectra. Under optimal experimental conditions, the CL response is proportional to the concentration of ketoprofen over the range of 2.0×10(-7) to 1.0×10(-5)mol/L with a correlation coefficient of 0.9950 and a detection limit of 8.0×10(-9)mol/L (3σ). The relative standard deviation for 11 repetitive determinations of 1.0×10(-6)mol/L ketoprofen is 2.9%. The utility of this method was demonstrated by determining ketoprofen in pharmaceutical formulations without interference from its potential impurities. 相似文献
6.
流动注射抑制化学发光法测定吲达帕胺 总被引:1,自引:0,他引:1
在碱性介质中,吲达帕胺对N 溴代琥珀酰亚胺(NBS) 荧光素发光体系具有很强的抑制作用,并且其抑制化学发光强度与吲达帕胺的质量浓度在一定范围内呈良好的线性关系,基于此,建立了测定痕量吲达帕胺的流动注射化学发光分析法。线性范围为0.01~5.0μg mL,检出限为(3σ)4.7ng mL,对0.1μg mL的吲达帕胺连续测定11次,相对标准偏差为2.6%。将本方法用于片剂中吲达帕胺的分析,并与药典标准方法测定结果一致。 相似文献
7.
A simple, rapid and sensitive method for the determination of two fluoquinolones (FQs), enoxacin (ENX) and ofloxacin (OFLX) is described by using flow injection analysis with potassium permanganate-sodium sulfite chemiluminescence detection. The calibration graphs for ENX and OFLX are linear in the range of 8.0×10−10-1.0×10−5 and 1.0×10−9-1.0×10−6 mol l−1, respectively. The 3σ limits of detection are 2.4×10−10 mol l−1 for ENX and 5.6×10−10 mol l−1 for OFLX. The method is applied satisfactorily to the determination of the two FQs in dosage form and urine sample. The possible mechanism is also proposed. 相似文献
8.
Chemiluminescent reagents for the determination of residual ozone were compared. Each method was automated using gas diffusion flow injection analysis. The luminol method gave a four order of magnitude working range with an LOD of 0.008 mg O(3)/l. The luminol method has better analytical characteristics than the standard colorimetric Indigo Blue methods. 相似文献
9.
流动注射化学发光法测定青霉素G钾 总被引:1,自引:0,他引:1
在甲醛的存在下,酸性KMnO4与青霉素G钾能够产生很强的化学发光,从而建立了KMnO4-甲醛-青霉素G钾化学发光体系来测定青霉素G钾.青霉素G钾的测定线性范围为2.0×10-7~1.0×10-5 g/mL,方法的检出限为1.4×10-7 g/mL,对4.0×10-7 g/mL的青霉素G钾进行11次平行测定,相对标准偏差为1.0%,用此法测定青霉素G钾取得了较好的结果. 相似文献
10.
盐酸头孢吡肟(Cefepime Hydrochloride)作为第四代头孢菌素类抗菌新药,具有高效、广谱、低毒、耐细菌β-内酰胺酶等特点。目前测定盐酸头孢吡肟的方法有液相色谱[1]、高效液相色谱法[2-7]、红外与X-射线[8]等。本文研究发现,在氢氧化钠介质中,盐酸头孢吡肟可被N-溴代琥珀酰亚胺(NBS)氧化产生微弱的化学发光,在荧光素共存时产生较强的化学发光,其发光强度与盐酸头孢吡肟的质量浓度在0·05mg·L-1~10mg·L-1范围内呈良好的线性关系。基于此,结合流动注射技术,建立了测定盐酸头孢吡肟的流动注射化学发光方法。该法的检出限(3σ)为29μg·L-… 相似文献
11.
12.
流动注射化学发光增强法测定巯嘌呤的应用研究 总被引:4,自引:0,他引:4
试验发现6-巯基嘌呤对Luminol-H2O2-OH-体系的化学发光有较强的增敏作用。据此建立了一种流动注射化学发光增强法测定6-巯基嘌呤的新方法。该法测定6-巯基嘌呤的检出限为7.5×10-9mol/L,线性范围为2.0×10-8~2.5×10-6mol/L,对6.0×10-7mol/L的6-巯基嘌呤测定的相对标准偏差为1.6%(n=11),已用于合成样中6-巯基嘌呤的测定。 相似文献
13.
A flow injection system coupled with two simple and sensitive chemiluminescence (CL) methods is described for the determination
of some phenolic compounds. The methods are based on the inhibition effects of the investigated phenols on the CL signal intensities
of N-chlorosuccinimide-KI-luminol (NCS-KI-luminol) and NCS-luminol systems. The influences of the chemical and hydrodynamic parameters
on the decrease in CL signal intensities of NCS-KI-luminol and NCS-luminol systems for hydroquinone, catechol, and resorcinol,
serving as the model compounds of analyte, were studied in the flow injection mode of analysis. Under the selected conditions,
the proposed CL systems were used for the determination of some phenolic compound and analytical characteristics of the systems
including calibration equation, correlation coefficient, linear dynamic range, limit of detection, and sample throughput.
The limits of detection for hydroquinone, catechol, and resorcinol were 0.002, 0.01, and 0.3 μM using the NCS-KI-luminol system;
for the NCS-luminol system these were 0.01, 0.17, and 1.6 μM, respectively. The relative standard deviation for 10 repeated
measurements of 0.04, 0.06, and 1 μM of hydroquinone, catechol, and resorcinol were 1.9, 1.4, and 2.0%, respectively, with
the NCS-KI-luminol system; for 0.2, 0.5, and 4 μM of hydroquinone, catechol, and resorcinol these were 2.6, 2.2, and 3.7%,
respectively, using the NCS-luminol system. The method was applied to the determination of catechol in known environmental
water samples with a relative error of less than 6%. A possible reaction mechanism of the proposed CL system is discussed
briefly.
相似文献
14.
15.
A simple chemiluminometric method using flow injection has been developed for the determination of paracetamol (acetaminophen), based on the chemiluminescence produced by the reduction of tris(2,2′-bipyridyl)ruthenium(III). The latter is obtained by oxidation of tris(2,2′-bipyridyl)ruthenium(II) by potassium permanganate in dilute sulphuric acid in the presence of paracetamol. A standard or sample solution was injected into the ruthenium(II) stream (flow rate 1.5 ml min−1) which was then merged with potassium permanganate in dilute sulphuric acid stream (flow rate 0.5 ml min−1). The chemiluminescence intensity is enhanced by the presence of manganese(II) ions. Under the optimum conditions, a linear calibration graph was obtained over the range of 0.3-50.0 μg ml−1 and the detection limit was 0.2 μg ml−1 (s/n = 3). The relative standard deviation of the proposed method calculated from 20 replicate injections of 5.0 μg ml−1 paracetamol was 1.1%. The sample throughput was 90 h−1. The method was successfully applied to the determination of paracetamol in commercial pharmaceutical formulations. 相似文献
16.
17.
18.
Based on the chemiluminescence (CL) emission generated from the oxidation of ceftriaxone sodium alkali hydrolysate by potassium permanganate in polyphosphoric acid (PPA), a novel determination method for ceftriaxone sodium was developed by using a flow-injection technique. The calibration curve appears to be linear in the range between 0.05 and 100 microg mL(-1) with a detection limit (3sigma) of 25 ng mL(-1), and a relative standard deviation (RSD) of 0.6% for eleven replicate determinations of 5.0 microg mL(-1) ceftriaxone sodium. The proposed method has been successfully utilized for the determination of ceftriaxone sodium in pharmaceutical formulations, while the chemiluminescence reaction mechanisms were investigated. 相似文献
19.
A photochemical method for the determination of lactate using a flow-injection system is proposed. The method is based on the decomposition of lactate in the presence of UO2(2+) and Fe3+ upon irradiation with UV or visible light. The Fe2+ produced in the photochemical process was monitored by measuring the absorbance after complexation with ferrozine (lambda max = 562 nm) or the chemiluminescence (CL) intensity in a luminol system without added oxidant. The range of measurements depended on the length of the irradiation time and the detection system used. The detection limits using CL and photometric detection were 2 ng ml-1 and 50 ng ml-1, respectively. The sample throughput was 45 samples h-1. The usefulness of the method was demonstrated by determining lactate levels in blood serum, milk, yoghurt, beer and pharmaceutical preparations. 相似文献
20.
在酸性介质中,木犀草素在KMnO4-HCHO体系中产生化学发光,据此建立了一种简便、快速测定木犀草素的化学发光新方法。化学发光强度与木犀草素的浓度在5.0×10-8~1.5×10-5g/mL范围内呈现出良好的线性关系。其检测限(3σ)为3.0×10-8g/mL,对8.0×10-7g/mL的木犀草素溶液进行11次平行测定,相对标准偏差为2.8%。方法可应用于实际样品和合成样品中木犀草素的测定。并探讨了反应的机理。 相似文献