人低密度脂蛋白~125Ⅰ固相标记──Iodogen法

本文报道了利用固相氧化剂１，３，４，６，—四氯—３ａ，６ａ，－二苯基甘脲（Ｉｏｄｏｇｅｎ）对人血清低密度脂蛋（ＬＤＬ）进行１２５Ⅰ标记的方法，结果表明：１２５Ｉ－ＬＤＬ的比放射性为１８５～５１８ｃｐｍ／ｎｇＬＤＬ，游离１２５Ⅰ含量在０．２７～１．７５％之间，脂质标记率＜１％，１２５Ｉ－ＬＤＬ浓度２５０～７５０μｇ／ｍｌ，１２５Ｉ－ＬＤＬ的生物活性经受体实验证实与天然脂蛋白一致．符合脂蛋白代谢研究的要求．本法具有反应条件温和，不易损伤脂蛋白，脂质标记率低．固相标记，不需要复杂设备等优点，为进行脂蛋白受体的研究提供了可靠的方法．     

人低密度脂蛋白^125I固相标记—Iodogen法

本文报道了利用固相氧化剂１，３，４，６，－四氯－３α、６α－二苯基甘脲（Ｉｏｄｏｇｅｎ）对人血清低密度脂蛋（ＬＤＬ）进行＾１２５Ｉ标记的方法，结果表明：＾１２５Ｉ－ＬＤＬ的比放射笥为１８５－５１８ｃｐｍ／ｎｇＬＤＬ，游离＾１２５Ｉ含量在０．２７－１．７５％之间，脂质记率＜１％，＾１２５Ｉ－ＬＤＬ浓度２５０－７５０μｇ／ｍｌ＾１２５Ｉ－ＬＤＬ的生物活性受体实验证实与天然与天然４脂蛋白一致，符合脂蛋白     

A NOVEL CYCLODIMERIZATION OF α，β－ UNSATURATED KETONES PROMOTED BY LOW－VALENT TITANIUM

Ａ ＮＯＶＥＬ ＣＹＣＬＯＤＩＭＥＲＩＺＡＴＩＯＮ ＯＦ α，β－ＵＮＳＡＴＵＲＡＴＥＤ ＫＥＴＯＮＥＳ ＰＲＯＭＯＴＥＤ ＢＹ ＬＯＷ－ＶＡＬＥＮＴ ＴＩＴＡＮＩＵＭ￥ＬｏｎｇＨｕＺＨＯＵ；ＧｕｉＹｕａｎＤＡＩ；ＤａＱｉｎｇＳＨＩ（Ｄｅｐａｒｔｍｅ...     

MALDI－TOF－MS法分析人血清白蛋白

用基质辅助激光解吸附飞行时间质谱（ＭＡＬＤＩ－ＴＯＦ－ＭＳ）法分析测定了人血清白蛋白。结果表明，全军血液制品研究中心应用Ｒｉｖａｎｏｌ和Ｃｏｈｎ＇ｓ两种方法分离提取的人血清白蛋白，其分子量与理论计算值相吻合，其纯度与市售标准人血清白蛋白相一致，用ＭＡＬＤＩ－ＴＯＦ－ＭＳ法分析鉴定人血清白蛋白，准确、简便和灵敏（仅需ＰＭ浓度样品），且重复性好，日内和日间差的变异系数均小于０．０３％，是ＳＤＳ－ＰＡＧＥ电泳法所无法比拟的。     

THEORETICAL STUDIES ON THE CYCLOADDITION REACTION OF FLUOROKETENE WITH ETHYLIDENIMINE

ＴＨＥＯＲＥＴＩＣＡＬＳＴＵＤＩＥＳＯＮＴＨＥＣＹＣＬＯＡＤＤＩＴＩＯＮＲＥＡＣＴＩＯＮＯＦＦＬＵＯＲＯＫＥＴＥＮＥＷＩＴＨＥＴＨＹＬＩＤＥＮＩＭＩＮＥＴＨＥＯＲＥＴＩＣＡＬＳＴＵＤＩＥＳＯＮＴＨＥＣＹＣＬＯＡＤＤＩＴＩＯＮＲＥＡＣＴＩＯＮＯＦＦＬＵ...     

热致液晶（LC70）／PET共混物的结构与性能

采用ＷＡＸＤ、ＤＳＣ、ＰＯＭ、ＳＥＭ和力学测试方法研究了ＬＣ７０／ＰＥＴ原位复合材料的结构与性能。结果表明,当ＬＣ７０／ＰＥＴ＜２０％时,ＬＣ７０对ＰＥＴ的结晶生长具有一定促进作用;当ＬＣ７０／ＰＥＴ＞３０％时,共混物结晶能力迅速下降,结晶放热和熔融焓明显降低。     

SYNTHESIS OF POLYSUBSTITUTED AROMATIC COMPOUNDS 3.THE DIELS-ALDER REACTION OF PHENYLSELENO SUBSTITUTED DIENES WITH DIMETHYL ACETYLENEDICARBOXYLATE

ＳＹＮＴＨＥＳＩＳ　ＯＦ　ＰＯＬＹＳＵＢＳＴＩＴＵＴＥＤ　ＡＲＯＭＡＴＩＣ　ＣＯＭＰＯＵＮＤＳ　３．ＴＨＥ　ＤＩＥＬＳ－ＡＬＤＥＲ　ＲＥＡＣＴＩＯＮ　ＯＦ　ＰＨＥＮＹＬＳＥＬＥＮＯ　ＳＵＢＳＴＩＴＵＴＥＤ　ＤＩＥＮＥＳ　ＷＩＴＨ　ＤＩＭＥＴＨＹＬ　Ａ...     

STUDY ON FINITE ELEMENT METHOD APPLIED TO HARTREE－FOCK－SLATER CALCULATIONS FOR LINEAR MOLECULES

ＳＴＵＤＹＯＮＦＩＮＩＴＥＥＬＥＭＥＮＴＭＥＴＨＯＤＡＰＰＬＩＥＤＴＯＨＡＲＴＲＥＥ－ＦＯＣＫ－ＳＬＡＴＥＲＣＡＬＣＵＬＡＴＩＯＮＳＦＯＲＬＩＮＥＡＲＭＯＬＥＣＵＬＥＳ￥ＦｅｉＷｕＣＨＥＮ；ＺｈｉＤａＣＨＥＮ；ＬｅＭｉｎＬＩ（Ｄｅｐａｒｔｍｅｎｔｏｆ...     

基质辅助激光解吸电离飞行时间质谱用于高分子端基修饰反应的监控

1　引　　言基质辅助激光解吸电离飞行时间 (ＭＡＬＤＩ ＴＯＦ)质谱是近年来发展起来的一种“软电离”质谱分析方法 ,具有样品分子不易碎裂 ,准分子离子峰强的优点 ,在多肽、蛋白质、ＤＮＡ等生物大分子及杯芳烃等重要的有机化合物的分析中得到广泛的应用。但实践证明 ,ＭＡＬＤＩ ＴＯＦ质谱还是高分子化合物的有效表征和结构鉴定手段。本文将ＭＡＬＤＩ ＴＯＦ质谱用于监控高分子化合物甲基丙烯酸丁酯末端酯基的水解反应的监控。2　实验部分2 .1　仪器和试剂　ＢｉｆｌｅｘＩＩＩ型ＭＡＬＤＩ ＴＯＦ质谱仪 (ＢｒｕｋｅｒＤａｌｔｏｎｉ…     

STUDY ON THE RELATIONSHIP BETWEEN MELTING POINT AND ACID DENATURING ACIDITY OF DNA BY ULTRAVIOLET SPECTROMETER

ＳＴＵＤＹ　ＯＮ　ＴＨＥ　ＲＥＬＡＴＩＯＮＳＨＩＰ　ＢＥＴＷＥＥＮ　ＭＥＬＴＩＮＧ　ＰＯＩＮＴ　ＡＮＤ　ＡＣＩＤ　ＤＥＮＡＴＵＲＩＮＧ　ＡＣＩＤＩＴＹ　ＯＦ　ＤＮＡ　ＢＹ　ＵＬＴＲＡＶＩＯＬＥＴ　ＳＰＥＣＴＲＯＭＥＴＥＲＳＴＵＤＹＯＮＴＨＥＲＥＬＡＴ...     

The effect of Lycium barbarum polysaccharide on alcohol-induced oxidative stress in rats

The aim of this study was to investigate the effects of Lycium barbarum polysaccharide (LBP) on alcohol-induced liver damage in rats. A total of 36 rats were divided into control, ethanol and ethanol + LBP groups. Rats in the ethanol group were fed 7 g ethanol/kg body weight by gastric infusion, three times a day, for 30 consecutive days, while rats in the control group received the same volume of physiological saline instead of ethanol, and rats in ethanol + LBP group were fed both ethanol (7 g/kg body weight) and LBP (300 mg/kg body weight/day). Alcoholic liver injury was examined by serum ALT and AST activities, alcoholic fatty liver was assessed by lipid levels, and oxidative stress was evaluated by SOD, CAT, GSH-Px, GSH and MDA assays. In the ethanol group, a significant elevation of enzymes and lipid in serum, increased MDA level and depletion of SOD, CAT, GSH-Px and GSH in liver were observed. LBP administration significantly ameliorated liver injury, prevented the progression of alcohol-induced fatty liver, and improved the antioxidant functions when compared with the ethanol group. Histopathological examination of rat liver revealed that LBP administration protected liver cells from the damage induced by ethanol. The results suggest that LBP is a promising agent to protect the liver from hepatotoxicity and fatty liver induced by ethanol intake.     

老年髋部骨折患者血清中宏量及微量元素含量分析

为探讨微量元素与骨质疏松症及髋部骨折的关系,采用原子吸收分光光度计等方法分别测定了40例老年髋部骨折患者血清中微量元素铜(Cu)、锌(Zn)、硒(Se)、铁(Fe)、锰(Mn)及宏量元素钙(Ca)、磷(P)、镁(Mg)、钾(K)、钠(Na)的含量,并与30例青壮年髋部骨折患者对照。结果表明,老年髋部骨折患者血清中的Ca含量明显升高(P<0.01)、Cu、Mn有显著提高(P<0.05),其它元素有升高趋势,但无显著性差异,Zn的含量明显降低(P<0.01)。测定血清中宏量及微量元素含量可能对预测髋部骨折及骨质疏松症的疗效评价有一定临床价值。     

Antioxidant activities of protein-enriched fraction from the larvae of housefly, Musca domestica

The protein-enriched fraction (PEF) was isolated and purified from the larvae of housefly, Musca domestica. This study was designed to investigate amino acid compositions, antioxidative effects and protective effects of PEF on red blood cell (RBC) hemolysis, lipid peroxidation. The effects of PEF treatment were studied on aged mice liver lipid peroxidation and antioxidant enzyme activities, which included superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Results: PEF not only inhibited H(2)O(2) stimulated oxidative hemolysis of erythrocytes of mice, but also depressed malondialdehyde (MDA) production in mice liver homogenate by auto-oxidation and hepatic mitochondria expanded induced by Fe(2+)-ascorbic acid system. Compared to control group, treatments of PEF significantly increases SOD and GSH-Px activity of serum and liver homogenate in aged mice. MDA level of serum and liver homogenate decreased significantly in aged mice. In conclusion, this study demonstrates that PEF possesses antioxidative activity and might be a valuable source of natural antioxidative agents.     

菀葆牌硒蜜康对酒精性肝损伤保护作用的实验研究

为研究菀葆牌硒蜜康对小鼠酒精性肝损伤的保护作用,将动物随机分成溶剂对照组、溶剂模型组、蜂蜜对照组、蜂蜜模型组及低、中、高3个样品剂量组,共7组,每组10只,3个样品剂量组每天给予硒蜜康1667、3333、6667mg／（kg·d）,连续给予小鼠灌胃30d后,模型对照组和样品剂量组最后一次给予受试物后20min,按体质量计一次灌胃给予50％乙醇10mL／kg,对照组给双蒸水,禁食12h后处死,取动物肝脏,测定肝匀浆液MDA、GSH、TG含量;肝脏冰冻切片,进行病理组织学检查。结果表明,与蜂蜜模型组比较,中、低剂量组的GSH含量升高,各剂量组的TG含量降低,各剂量组冰冻病理学组织切片观察到脂肪变性评分低于模型对照组,差异均有显著性。可见菀葆牌硒蜜康对酒精性肝损伤具有辅助保护作用。     

肝硬化患者血清微量元素与免疫功能的相关性研究

为探讨肝硬化患者血清中微量元素含量与细胞免疫功能的相关性,将70例肝硬化患者根据代偿期及失代偿期分为两组,30例健康体检者为对照组,用日立7170A型全自动生化分析仪检测了血清中的镁、铁、铜、锌含量,流式细胞技术检查了外周血CD3+、CD4+、CD8+、NK细胞。结果表明,肝硬化患者血清镁、铁、锌水平显著低于对照组;血清铜水平显著高于对照组;CD3+、CD4+、NK细胞低于正常对照组,以失代偿期改变明显。肝硬化患者血清铜水平与CD3+、CD4+、NK细胞呈负相关,血清锌水平与CD3+、CD4+、NK细胞呈正相关。提示肝硬化患者微量元素代谢异常与细胞免疫功能下降有一定的联系。     

多层螺旋CT灌注成像评价介入性热化疗治疗中晚期肝癌的价值

为探讨多层螺旋CT灌注成像(CT perfusion imaging,CTPI)在评价介入性热化疗对中晚期肝癌疗效方面的应用价值,本文分析了72例采用介入性热化疗的中晚期肝癌患者在治疗前后的CTPI灌注参数和血清肿瘤学标志物水平。通过与治疗前数据比较,发现治疗后癌灶区CTPI灌注参数明显改善,血清肿瘤标志物明显降低。CTPI灌注参数、血清肿瘤学标志物在碘油完全沉积和部分沉积组、客观缓解和未缓解组均表现出显著的统计学差异。Pearson相关分析结果显示CTPI灌注参数与肝癌血清肿瘤标志物之间存在显著相关性。由此可见,CTPI灌注参数可有效反映介入性热化疗前后晚期肝癌患者的动脉血供变化,为临床疗效评估提供有价值的参考。     

内蒙古牧区老年冠心病患者血清Zn,Cu,Ca,Mg的测定及临床意义

用薄样Ｘ射线荧光光谱法测定较区９１老年冠心病患者及６３名老年健康人血清Ｚｎ、Ｃｕ、Ｃａ、Ｍｇ、四种元素的含量。结果表明：牧区老年冠心病患者血清中Ｚｎ、Ｃｕ、含量与健康组比较无显著差异，但两者的比值高于对照组，可能与牧区牧民饮食习惯有关；老年冠心病组血清Ｃａ、Ｍｇ含量显著低于对照组。     

NAFLD患者TE成像特点与其病情严重程度及胰岛素抵抗的关系研究

本研究旨在分析NAFLD(非酒精性脂肪性肝病)患者TE(瞬时弹性成像技术)成像特点与其病情严重程度及胰岛素抵抗的关系。通过选择我院NAFLD患者112例,健康体检的患者108例,对比两组患者基线资料,将NAFLD患者根据病情分组并对比相关资料。研究表明NAFLD组患者肝功能、血清胰岛素、HOMA-IR(胰岛素抵抗指数)显著高于对照组,差异显著(P<0.05)。随着脂肪肝严重程度增加,患者普遍年龄增高,男性多于女性,肝脏硬度、肝功能、胰岛素相关指标均明显上升,差异显著(P<0.05)。ROC曲线分析表明CAP(受控衰减参数)对轻度、中度和重度NAFLD均有较高的诊断价值,灵敏度分别为0.865、0.861、0.931,特异度分别为0.614、0.819、0.822。随着NAFLD病情严重程度的加重,CAP与HOMA-IR值均升高,呈显著正相关(r=0.536,r=0.479)。据此可推断NAFLD患者的TE成像特点与不同程度NAFLD密切相关,胰岛素抵抗可临床辅助诊断NAFLD。     

老年慢性支气管炎急性发作期病人头发微量元素的测定与分析

为观察老年慢性支气管炎急性发作期头发中微量元素的变化,测定了50例老年慢性支气管炎急性发作期患者和40例正常对照组头发中锌、铁、铜、钙、锰、铅6种元素含量。结果表明,慢性支气管炎急性发作期患者发中锌、铁、铜、钙含量明显低正常对照组（P＜0．01）。提示头发中微量元素减少与慢性支气管炎易反复感染有一定的关系。     

补中益气汤对肝硬化患者机体微量元素的影响

为探讨补中益气汤对肝硬化患者机体微量元素水平的影响,采用全自动生化分析仪检测了30例服用补中益气汤肝硬化患者及30例肝硬化对照组患者血清中镁、铁、铜、锌的水平。结果表明,服用补中益气汤患者血清镁、铁、锌水平均显著高于对照组患者(P0.05),服用补中益气汤患者血清铜水平均显著低于对照组患者(P0.05)。提示了补中益气汤能显著提高肝硬化患者机体微量元素水平,对改善患者肝脏功能具有一定的作用。