精制蝮蛇抗栓酶对人体内锌铜铁的影响

对126例脑血栓形成患者用精制蝮蛇抗栓酶（Svate-3)治疗,探讨了用药前后头发微量元素锌、铜、铁的变化。结果表明,用药后人体内Zn含量高地用药前,且有显著性差异（P＜0.01)。Zn/Cu比值降低。     

脑血栓形成病人用精制蝮蛇抗栓酶治疗前后微量元素和体液免疫的变化及临床疗效的观察

用精制蝮蛇抗栓酶(Svate-3)治疗了108例急性期脑血栓形成病人,治疗前后分别测定病人血清微量元素锌、铜、铬、锰、硒及体液免疫指标 IgG, IgA, IgM及循环免疫复合物(CIC), 同时设定对照组观察 Svate-3的临床疗效.观察结果表明,治疗组疗效优于对照组.测定结果表明,Svate-3能使脑血栓病人血清 Zn增加(P＜0.05),血清 Cu减少(P＜0.05 ), Zn/Cu比值增加(P＜0.05),Svate-3可使体液免疫 IgA增加(P＜0.01) .     

氨基酰化酶的固定化和苯丙氨酸消旋体的拆分

本文合成了一种功能高分子材料,利用其适宜的物理性质和匹配的功能基来固定氨基酰化酶,并进行苯丙氨酸消旋体的拆分,研究结果表明:这种载体对氮基酰化酶蛋白固定容量大,固定化酶比活力高,活力损失小,本文系统地研究了氮基酰化酶拆分苯丙氨酸消旋体的理化性质,包括温度、pH、离子浓度、热稳定性、激活离子、底物浓度以及蛋白变性剂等,并进行苯丙氨酸消旋体的连续拆分,对拆分产物采用离子交换法进行分离精制,真空浓缩得到L-苯丙氨酸的盐酸盐,产率高,对产品进行旋光检验,纯度高。     

球状苯基纤维素疏水性吸附树脂分离生物活性蛋白质的研究

本文考察了球状苯基纤维素疏水性吸附树脂对三种生物活性蛋白质：精制木瓜酶、中性蛋白酶和菠萝蛋白的吸附分离情况。研究表明,该树脂对菠蛋白酶的吸附分离情况较好,一步富集倍数和活性回收率高,活性回收率达１２８％,浓缩倍数达４．４,进一步盐析干燥可制得固体晶状菠萝酶。     

血液灌流用内毒素吸附材料研究

内毒素血症是败血症致死率极高的主要原因之一,迅速并有效地清除患者体内的内毒素,是临床医学面临的一个难题．用血液灌流的方法,选用有效的吸附剂,通过体外循环从血液中直接清除内毒素,受到了人们越来越多的关注．国内外研究者已开始研究利用该方法治疗内毒素血症,用活性炭     

肝硬化合并低氧血症31例临床研究

为探讨肝硬化患者的血氧变化与临床关系，以肝炎后肝硬化患者31例为观察组（A组），采用血气分析仪测定其动脉血氧分压（PaO2)及动脉血氧饱和度（SaO2)，并以正常人15例作对照组（B组）进行比较结果表明，A组的低氧血症发生率为41％，B组为0。肝硬化患者低氧血症的发生率高，其严重程度与Child-Pugh分级有关。早期发现低氧血症并予以治疗有助于改善肝硬化患者的预后。     

基于特征肽的超高效液相色谱-串联质谱法检测矛头蝮蛇蛇毒种属来源及类凝血酶含量

蛇毒血凝酶类药物是以蝮蛇蛇毒为原料制备的止血药,主要活性成分为蛇毒类凝血酶(svTLEs)。不同蛇种来源的svTLEs结构不同,止血机制不同,药理作用也存在差异,因此准确鉴别蛇毒种属来源和svTLEs含量对于保障该类产品的质量至关重要。研究基于蛋白质组学技术,筛选出了具有种属特异性的矛头蝮蛇svTLE特征肽,并建立了基于特征肽的超高效液相色谱-串联质谱(UHPLC-MS/MS)检测矛头蝮蛇蛇毒种属来源及类凝血酶含量的方法。采用胰蛋白酶对纯化的矛头蝮蛇svTLE进行酶解,利用纳升液相色谱-四极杆/静电场轨道阱高分辨质谱(Nano LC-Q-Exactive-MS)和Proteome Discoverer 2.2软件分别进行多肽的检测和鉴定,通过BLAST搜索与Uniprot数据库对比分析,筛选出具有种属特异性的矛头蝮蛇svTLEs特征肽“EAYNGLPAK”。针对该特征肽对酶解温度、酶解时间和酶用量等样品前处理方法进行了优化,利用超高效液相色谱-串联质谱,以m/z 481.9>315.2和481.9>485.2作为检测离子对,采用ESI⁺模式进行了多反应...     

氨基酰化酶的固定化及其对D,L—蛋氨酸的光学拆分

本文用大孔网高分子载体对氨基酰化酶进行固定化,并系统地研究了温度、pH值、离子浓度、底物浓度、激活离子及蛋白质变性剂对固定化酶活力的影响。由于载体的物理性质和功能基较适宜,因而吸附蛋白质的容量大,使固定化酶的活力和活力回收率都高。用固定化酶柱对D,L—蛋氨酸进行连续拆分,酶柱使用周期长,稳定性高。拆分的产物用离子交换法进行分离与精制后,可制得纯度高,产率理想的L—蛋氨酸盐酸盐。     

高效蛋白质浓缩剂的制备及浓缩作用的研究

采用自由基水溶液聚合方法合成了超高分子量的丙烯酸（钠）聚合物。该聚合物具有高达几十倍至上千倍的吸水能力，通过适当控制聚合物的交联度使其成为具有一定孔径的网状物，可用于生物大分子，如：蛋白质溶液或其它生物提取液的浓缩。试验了用反透析法浓缩胰蛋白酶的方法，与直接加入浓缩法相比，能够更好地保持酶的活性。与传统的浓缩方法相比，用该方法浓缩蛋白质具有效率高，浓缩剂用量少的优点。     

HA—2型大孔吸附剂用于血液灌流／血液透析联合清除中分子物质、治疗流行性出血热并发急性肾衰的临床应用研究

本文研究了ＨＡ—２型大孔吸附剂用于血液灌流治疗流行性出血热并发急性肾衰，观察其血液相容性及疗效。     

Influence of parenteral fat emulsion Intralipos and citric acid on blood viscosity and erythrocyte morphology in vitro

In most studies fat emulsion had been administered by enteral route. Recently a parenteral soybean-oil emulsion has been developed. In this study we assessed the effects of a parenteral soybean-oil emulsion (Intralipos((R))) and citric acid on blood rheology and erythrocyte morphology in vitro. Porcine blood was incubated in vitro with increasing concentrations of fat emulsion Intralipos((R)) and citric acid at 37 degrees C for 1h. Viscosity of plasma and whole blood was measured using a FASCO-2050 digital viscometer. Red blood cell morphology was examined by light microscopy. The viscosity of whole blood represented an ascending dose-dependence for different Intralipos((R)) concentrations at high shear rate of 90 and 225s(-1), however, it decreased with citric acid concentrations. On the other hand, the whole blood viscosity also declined with citric acid concentrations in presence of 30% Intralipos((R)) (v/v), and there is a minimal viscosity at 0.67% citric acid (v/v). There are some thorns on the blood membrane at 40% Intralipos((R)) as compared with control (no Intralipos((R)) addition), which indicates the Intralipos((R)) compound may affect blood cell membranes, and resulted in whole blood viscosity increase. We concluded that the intravenous soybean-oil preparation Intralipos((R)) interacts with the erythrocyte membrane, and citric acid could alleviate the whole blood viscosity.     

JJG 1002-2005旋转黏度计计量检定规程存在的问题探讨

对JJG 1002-2005旋转黏度计计量检定规程存在的问题进行探讨,指出该规程不适合血液黏度计的检定,应重新制订血液黏度计计量检定规程或计量校准规范,以适应医学临床需要.     

Two-photon dual-channel fluorogenic probe for in situ imaging the mitochondrial H2S/viscosity in the brain of drosophila Parkinson’s disease model

H₂S is an essential gas signal molecule in cells, and viscosity is a key internal environmental parameter. Recent studies have shown that H₂S acts as a cytoarchitecture agent and gas transmitter in many tissues, e.g., as a regulator of neuroendocrine in the brain for mediating vascular tone in blood vessels. Mitochondrial viscosity is an important parameter for judging whether mitochondrial function is normal. It has been reported that oxidative stress and mitochondrial dysfunction are connected with Parkinson’s disease (PD), and the protective role of H₂S in PD models has been extensively demonstrated. Herein, Mito-HS, a new two-photon fluorescent probe was demonstrated to detect cross-talk between the two channels of mitochondrial viscosity and H₂S content. Moreover, this probe could detect the relative amount of and changes in mitochondrial H₂S in situ due to the reduced mitochondrial targeting ability after reaction with H₂S. The results show that H₂S in mitochondria is inversely related to viscosity. The PD model has a lower H₂S in mitochondria and a higher mitochondrial viscosity than did the normal. This result is important for our deep understanding of PD and its causes.     

Effects of Tao‐Hong‐Si‐Wu decoction on acute blood stasis in rats based on a LC‐Q/TOF‐MS metabolomics and network approach

A novel approach using metabolomics coupled with a metabolic network was used to investigate the effects of Tao‐Hong‐Si‐Wu decoction (THSWD) on the rat model of acute blood stasis syndrome. Acute blood stasis syndrome was induced by placing the rats in ice‐cold water following two injections with epinephrine. The hemorheological indicators [whole blood viscosity (WBV) and plasma viscosity (PV)] and the blood coagulation indicators [thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT) and fibrinogen (FIB)] were detected. The nonparametric univariate method and multivariate statistical analysis were performed for determining the potential biomarkers. A correlation map was structured between biochemical indicators and hub metabolites to explain the effects mechanism of THSWD. After the administration of THSWD, the levels of WBV, PV, TT, APTT and FIB returned to levels observed in the control group. According to metabolomics coupled with metabolic network analysis, the intervention of THSWD in rats with acute blood stasis syndrome induced substantial and characteristic changes in their metabolic profiles. Fifteen metabolites were screened, which mainly involved 10 pathways and five hub metabolites, namely, l ‐glutamate, l ‐phenylalanine, N‐acylsphingosine, arachidonic acid and phosphatidate. The biochemical indicators and hub metabolites could be adjusted to close to normal levels by THSWD. Therefore, combining metabolomics and metabolic network helped to evaluate the effects of THSWD on acute blood stasis.     

Effect of whole blood and plasma on the permeability of glucose through different cellulose and cellulose acetate membranes

Model experiments were made with a flow-through dialysis cell to study how glucose in whole blood, plasma and aqueous standards is transferred through different membranes. Detection was via a glucose dehydrogenase reaction and NADH monitoring with a chemically modified electrode. The detector response was linear with the glucose concentration in all cases. The non-Newtonian properties of whole blood seem to be of great importance for the response. Theoretical models are proposed both for the effect of plasma viscosity and the red cell volume fraction (haematocrit) dependence on the glucose transfer rate. The models could only partially explain the differences between measurements in aqueous solutions and body fluids. Better agreement and less haematocrit dependence were obtained with low-permeability membranes.     

表面张力对PEO稀溶液粘度行为的影响

测定了不同分子量聚氧化乙烯(PEO)在水和苯溶剂中的粘度,发现在低浓度区PEO水溶液的比浓粘度出现负偏离, PEO苯溶液比浓粘度与浓度之间依旧满足线性关系.表面张力测定结果表明, PEO分子显著降低了水的表面张力,而苯的表面张力则不受影响.PEO水溶液和纯溶剂水表面张力的不同干扰了高分子溶液和溶剂在粘度计中流过时间的测量,导致低浓度区PEO水溶液比浓粘度出现负偏离.利用PEO水溶液和水表面张力测定结果,结合乌式粘度计的几何尺寸,定量分析了PEO水溶液和纯溶剂水表面张力的差异对粘度测量结果的影响,计算结果与实验结果基本相符.如果用PEO水溶液流过时间对浓度作图的外推值t0*计算相对粘度,可以完全消除PEO水溶液和水表面张力差异对粘度测量的影响.     

Two-photon dual-channel fluorogenic probe for in situ imaging the mitochondrial H2S/viscosity in the brain of drosophila Parkinson’s disease model

H₂S is an essential gas signal molecule in cells, and viscosity is a key internal environmental parameter. Recent studies have shown that H₂S acts as a cytoarchitecture agent and gas transmitter in many tissues, e.g., as a regulator of neuroendocrine in the brain for mediating vascular tone in blood vessels. Mitochondrial viscosity is an important parameter for judging whether mitochondrial function is normal. It has been reported that oxidative stress and mitochondrial dysfunction are connected with Parkinson’s disease (PD), and the protective role of H₂S in PD models has been extensively demonstrated. Herein, Mito-HS, a new two-photon fluorescent probe was demonstrated to detect cross-talk between the two channels of mitochondrial viscosity and H₂S content. Moreover, this probe could detect the relative amount of and changes in mitochondrial H₂S in situ due to the reduced mitochondrial targeting ability after reaction with H₂S. The results show that H₂S in mitochondria is inversely related to viscosity. The PD model has a lower H₂S in mitochondria and a higher mitochondrial viscosity than did the normal. This result is important for our deep understanding of PD and its causes.     

LW-1型增效剂对聚丙烯酰胺水溶液增粘的实验研究

研究了LW 1型增效剂对聚丙烯酰胺水溶液的增粘效果 ,考察了增效剂含量、溶液中金属离子浓度、剪切强度、聚合物浓度、pH值对增粘效果的影响 .结果表明 ,增效剂能起到显著的增粘效果 ,对提高原油采收率具有重大意义 .此外 ,初步分析了LW 1型增效剂对聚丙烯酰胺水溶液增粘的可能机理     

氟碳改性聚丙烯酰胺共聚物的溶液性质

研究了氟碳链改性的聚丙烯酰胺水溶液性质及其对盐浓度的依赖性，重点考察 了氟碳疏水组分含量、盐浓度对特性粘数的影响，以及粘度随剪切速率和温度的变 化关系。结果表明聚合物溶液的粘度随盐的引入而增大，但在两种溶液中最小值对 应的共聚单体含量一致。随温度的增加，共聚物溶液粘度依次出现了最小和最大值 。所有实验结果均归于溶液中存在的疏水和氢键两种相反作用的综合结果。     

Span 80 effect on the solvent extraction for heavy oil recovery

In this study, we used the Span 80 to enhance solvent extraction process, and we explored the mechanism. The results indicated different solvents would obtain different oil recovery, and toluene showed the optimal oil recovery, and the n-heptane showed the lowest oil recovery. The complex solvents could improve oil recovery. Toluene could make the heavy oil show the lowest viscosity (89.6 Pa.S), and n-heptane make the heavy oil show the highest viscosity (176.3 Pa.S). Complex solvents could decrease the heavy oil viscosities. The higher C/H was, the higher heavy oil recovery was, and when the asphaltene and resins content increase, the C/H would increase. The C/H showed the highest value (9.09, by toluene) and the lowest value (8.15). In this study, Span 80 could increase heavy oil C/H ratio, decrease heavy oil viscosity. Span 80 could make the sands surface more hydrophilic, and then the solvent loss would decrease. The oil recovery was high after 10 times recycle use.