共查询到20条相似文献,搜索用时 15 毫秒
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Dr. Vittorio Limongelli Stefano De Tito Dr. Linda Cerofolini Dr. Marco Fragai Dr. Bruno Pagano Dr. Roberta Trotta Dr. Sandro Cosconati Dr. Luciana Marinelli Prof. Ettore Novellino Prof. Ivano Bertini Prof. Antonio Randazzo Prof. Claudio Luchinat Prof. Michele Parrinello 《Angewandte Chemie (International ed. in English)》2013,52(8):2269-2273
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Dr. Vee Vee Cheong Dr. Christopher Jacques Lech Dr. Brahim Heddi Prof. Dr. Anh Tuân Phan 《Angewandte Chemie (International ed. in English)》2016,55(1):160-163
G‐quadruplexes are four‐stranded nucleic acid structures that are built from consecutively stacked guanine tetrad (G‐tetrad) assemblies. The simultaneous incorporation of two guanine base lesions, xanthine (X) and 8‐oxoguanine (O), within a single G‐tetrad of a G‐quadruplex was recently shown to lead to the formation of a stable G?G?X?O tetrad. Herein, a judicious introduction of X and O into a human telomeric G‐quadruplex‐forming sequence is shown to reverse the hydrogen‐bond polarity of the modified G‐tetrad while preserving the original folding topology. The control exerted over G‐tetrad polarity by joint X?O modification will be valuable for the design and programming of G‐quadruplex structures and their properties. 相似文献
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Direct and Single‐Molecule Visualization of the Solution‐State Structures of G‐Hairpin and G‐Triplex Intermediates 下载免费PDF全文
Dr. Arivazhagan Rajendran Prof. Dr. Masayuki Endo Kumi Hidaka Prof. Dr. Hiroshi Sugiyama 《Angewandte Chemie (International ed. in English)》2014,53(16):4107-4112
We present the direct and single‐molecule visualization of the in‐pathway intermediates of the G‐quadruplex folding that have been inaccessible by any experimental method employed to date. Using DNA origami as a novel tool for the structural control and high‐speed atomic force microscopy (HS‐AFM) for direct visualization, we captured images of the unprecedented solution‐state structures of a tetramolecular antiparallel and (3+1)‐type G‐quadruplex intermediates, such as G‐hairpin and G‐triplex, with nanometer precision. No such structural information was reported previously with any direct or indirect technique, solution or solid‐state, single‐molecule or bulk studies, and at any resolution. Based on our results, we proposed a folding mechanism of these G‐quadruplexes. 相似文献
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Miguel Martín‐Arroyo Anselmo del Prado Raquel Chamorro Nerea Bilbao David Gonzlez‐Rodríguez 《Angewandte Chemie (International ed. in English)》2020,59(23):9041-9046
In analogy to covalent reactions, the understanding of noncovalent association pathways is fundamental to influence and control any supramolecular process. Following an approach that is reminiscent of covalent methodologies, we study here, for the first time, the mechanism of G‐quadruplex formation in organic solvents. Our results support a reaction pathway in which the cation shifts the equilibrium towards a G‐quartet transient intermediate, which then acts as a template in the formation of the G‐quadruplex product. 相似文献
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Tao Li Erkang Wang Prof. Dr. Shaojun Dong Prof. 《Chemistry (Weinheim an der Bergstrasse, Germany)》2009,15(9):2059-2063
Base pairs, magic hands : An additional base‐pairing duplex is utilized to control the folding topologies of a bimolecular G‐quadruplex formed by two G‐rich single‐stranded DNAs (see picture), which is dependent on the position of base pairs. This study clearly reveals an important intrinsic role of additional base pairs in the G‐quadruplex structure, and also provides a clue to the formation mechanism of the G‐quadruplex‐based DNAzyme.
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This review deals with recent progress in the synthesis and evaluation of our telomestatin‐inspired macrocyclic polyoxazoles as G‐quadruplex (G4) ligands. The hexaoxazole derivatives (6OTDs) interact with and stabilize G4‐forming oligonucleotides, depending upon the character of the side chain functional groups. Cationic functional groups are particularly effective due to their secondary interaction with phosphate in the DNA backbone. On the other hand, heptaoxazole derivatives (7OTDs) showed potent G4‐binding and stabilization activity regardless of the functional groups on the side chain. A caged G4 ligand, Y2Nv2‐6OTD ( 7 ), and a fluorescent G4 ligand, L1BOD‐7OTD ( 13 ), have been synthesized. 相似文献
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Dr. Daniela Verga Dr. Florian Hamon Dr. Florent Poyer Dr. Sophie Bombard Dr. Marie‐Paule Teulade‐Fichou 《Angewandte Chemie (International ed. in English)》2014,53(4):994-998
We have developed a straightforward synthetic pathway to a set of six photoactivatable G‐quadruplex ligands with a validated G4‐binding motif (the bisquinolinium pyridodicarboxamide PDC‐360A) tethered through various spacers to two different photo‐cross‐linking groups: benzophenone and an aryl azide. The high quadruplex‐versus‐duplex selectivity of the PDC core was retained in the new derivatives and resulted in selective alkylation of two well‐known G‐quadruplexes (human telomeric G4 and oncogene promoter c‐myc G4) under conditions of harsh competition. The presence of two structurally different photoactivatable functions allowed the selective alkylation of G‐quadruplex structures at specific nucleobases and irreversible G4 binding. The topology and sequence of the quadruplex matrix appear to influence strongly the alkylation profile, which differs for the telomeric and c‐myc quadruplexes. The new compounds are photoactive in cells and thus provide new tools for studying G4 biology. 相似文献
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Dr. Els Pardon Dr. Cecilia Betti Dr. Toon Laeremans Dr. Florent Chevillard Dr. Karel Guillemyn Prof. Dr. Peter Kolb Prof. Dr. Steven Ballet Prof. Dr. Jan Steyaert 《Angewandte Chemie (International ed. in English)》2018,57(19):5292-5295
The conformational complexity of transmembrane signaling of G‐protein‐coupled receptors (GPCRs) is a central hurdle for the design of screens for receptor agonists. In their basal states, GPCRs have lower affinities for agonists compared to their G‐protein‐bound active state conformations. Moreover, different agonists can stabilize distinct active receptor conformations and do not uniformly activate all cellular signaling pathways linked to a given receptor (agonist bias). Comparative fragment screens were performed on a β2‐adrenoreceptor–nanobody fusion locked in its active‐state conformation by a G‐protein‐mimicking nanobody, and the same receptor in its basal‐state conformation. This simple biophysical assay allowed the identification and ranking of multiple novel agonists and permitted classification of the efficacy of each hit in agonist, antagonist, or inverse agonist categories, thereby opening doors to nanobody‐enabled reverse pharmacology. 相似文献
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Thi Hong Anh Truong Fernaldo Richtia Winnerdy Anh Tun Phan 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(39):13972-13977
A knot‐like G‐quadruplex peripheral structure is formed by a 7‐nt DNA sequence DL7 (TGTTGGT), in which six out of its seven nucleobases participate in compact base‐pairing interactions. Here, the solution NMR structure of a 24‐nt DNA oligonucleotide containing the DL7 sequence shows the interaction between a two‐layer anti‐parallel G‐quadruplex core and the peripheral knot‐like structure, including the construction of two sharp turns in the DNA backbone. The formation of this novel structural element highlights the intricate properties of single‐stranded DNA folding in presence of G‐quadruplex‐forming motifs. We demonstrated the compatibility of the DL7 knot‐like structure with various G‐quadruplexes, which could have implications in drug design and DNA engineering. 相似文献