共查询到20条相似文献,搜索用时 0 毫秒
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Dr. Michael Georg Hoesl M. Sc. Stefan Oehm Dr. Patrick Durkin Dr. Elise Darmon Dr. Lauri Peil Dr. Hans‐Rudolf Aerni Prof. Dr. Juri Rappsilber Prof. Dr. Jesse Rinehart Prof. Dr. David Leach Prof. Dr. Dieter Söll Prof. Dr. Nediljko Budisa 《Angewandte Chemie (International ed. in English)》2015,54(34):9726-9726
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Cover Picture: Debugging Eukaryotic Genetic Code Expansion for Site‐Specific Click‐PAINT Super‐Resolution Microscopy (Angew. Chem. Int. Ed. 52/2016) 下载免费PDF全文
Dr. Ivana Nikić Gemma Estrada Girona Jun Hee Kang Giulia Paci Sofya Mikhaleva Christine Koehler Dr. Nataliia V. Shymanska Camilla Ventura Santos Daniel Spitz Dr. Edward A. Lemke 《Angewandte Chemie (International ed. in English)》2016,55(52):15931-15931
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Dr. Takahito Mukai Dr. Anastasia Sevostyanova Dr. Tateki Suzuki Dr. Xian Fu Prof. Dieter Söll 《Angewandte Chemie (International ed. in English)》2018,57(24):7215-7219
Selenocysteine (Sec, U) confers new chemical properties on proteins. Improved tools are thus required that enable Sec insertion into any desired position of a protein. We report a facile method for synthesizing selenoproteins with multiple Sec residues by expanding the genetic code of Escherichia coli. We recently discovered allo‐tRNAs, tRNA species with unusual structure, that are as efficient serine acceptors as E. coli tRNASer. Ser‐allo‐tRNA was converted into Sec‐allo‐tRNA by Aeromonas salmonicida selenocysteine synthase (SelA). Sec‐allo‐tRNA variants were able to read through five UAG codons in the fdhF mRNA coding for E. coli formate dehydrogenase H, and produced active FDHH with five Sec residues in E. coli. Engineering of the E. coli selenium metabolism along with mutational changes in allo‐tRNA and SelA improved the yield and purity of recombinant human glutathione peroxidase 1 (to over 80 %). Thus, our allo‐tRNAUTu system offers a new selenoprotein engineering platform. 相似文献
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