Visualisation of mitochondria in living neurons with single- and two-photon fluorescence laser microscopy

In this work we investigated the relative merits of conventional single-photon confocal laser scanning fluorescence microscopy (CLSM) and two-photon laser scanning fluorescence microscopy (2p-LSM) for the study of mitochondria in living neurons. Dorsal root ganglion neurons were loaded with the mitochondrion-specific fluorescent dye JC-1, the ratio between red (J-aggregates) and green (monomer) fluorescence of which reflects mitochondrial membrane potential. Cells were illuminated at 488 nm for single-photon excitation or at 870 nm for two-photon excitation. In both modalities we found that mitochondria showed: (i) similar appearance; (ii) similar fluorescence ratio values over both whole cell bodies and individual mitochondria; and (iii) similar responses to mitochondrial uncoupler, which dropped the ratio values by 50%. However, 2p-LSM exhibited several advantages over CLSM: (i) better signal/noise ratio in the green emission channel; (ii) less phototoxicity upon repetitive scanning in the focal plane; and (iii) no significant loss of image quality upon repetitive scans in the z direction. We conclude that, while both techniques enable visualisation of individual mitochondria in living cells, 2p-LSM has significant advantages for physiological work requiring time-lapse experiments or four-dimensional reconstructions of mitochondria.     

Measurement of the branching fraction of Gamma(4S) --> B0B0

We report the first measurement of the branching fraction f(00) for Gamma(4S) --> B(0)B(0). The data sample consists of 81.7 fb(-1) collected at the Gamma(4S) resonance with the BABAR detector at the SLAC PEP-II asymmetric-energy e(+)e(-) storage ring. Using partial reconstruction of the decay B(0) --> D(*+) l(-)nu(l) in which only the charged lepton and the soft pion from the decay D(*+) --> D(0)pi(+) are reconstructed, we obtain f(00) = 0.487 +/- 0.010(stat) +/- 0.008(syst). Our result does not depend on the branching fractions of B(0) --> D(*+)l(-)nu(l) and D(*+) --> D(0)pi(+) decays, on the ratio of the charged and neutral B meson lifetimes, nor on the assumption of isospin symmetry.     

Fundamentals of high power pulsed magnetron sputtering: Visualization of mechanisms for rate reduction and increased ion fraction

High power pulsed magnetron sputtering (HPPMS) has attracted a great deal of interest because of its potential to provide a highly ionized flux of target material to the substrate. However, practical experience with HPPMS has shown deposition rates typically lower than direct current sputtering (DC sputtering) rates at the same average power, and ionization fractions of target material flux to the substrate often lower than anticipated. A mathematical model explaining the source of these observed process characteristics has been proposed by the author previously. Here, a graphical means of visualizing the mechanism for deposition rate reduction and lower than expected ionization fractions is presented.     

The fully entangled fraction as an inclusive measure of entanglement applications

Characterizing entanglement in all but the simplest case of a two qubit pure state is a hard problem, even understanding the relevant experimental quantities that are related to entanglement is difficult. It may not be necessary, however, to quantify the entanglement of a state in order to quantify the quantum information processing significance of a state. It is known that the fully entangled fraction has a direct relationship to the fidelity of teleportation maximized under the actions of local unitary operations. In the case of two qubits we point out that the fully entangled fraction can also be related to the fidelities, maximized under the actions of local unitary operations, of other important quantum information tasks such as dense coding, entanglement swapping and quantum cryptography in such a way as to provide an inclusive measure of these entanglement applications. For two qubit systems the fully entangled fraction has a simple known closed-form expression and we establish lower and upper bounds of this quantity with the concurrence. This approach is readily extendable to more complicated systems.     

Condensed fraction of an atomic Bose gas induced by critical correlations

We study the condensed fraction of a harmonically trapped atomic Bose gas at the critical point predicted by mean-field theory. The nonzero condensed fraction f(0) is induced by critical correlations which increase the transition temperature T(c) above T(c) (MF). Unlike the T(c) shift in a trapped gas, f(0) is sensitive only to the critical behavior in the quasiuniform part of the cloud near the trap center. To leading order in the interaction parameter a/λ(0), where a is the s-wave scattering length and λ(0) the thermal wavelength, we expect a universal scaling f(0) proportionally (a/λ(0))(4). We experimentally verify this scaling using a Feshbach resonance to tune a/λ(0). Further, using the local density approximation, we compare our measurements with the universal result obtained from Monte Carlo simulations for a uniform system, and find excellent quantitative agreement.     

Measurement of the B0-->J/psipi+pi- branching fraction

We present a measurement of the branching fraction for the decay of the neutral B meson into the final state J/psipi(+)pi(-). The data set contains approximately 56 x 10(6) BB pairs produced at the Upsilon(4S) resonance and recorded with the BABAR detector at the PEP-II asymmetric-energy e(+)e(-) storage ring. The result of this analysis is B(B0-->J/psipi(+)pi(-))=(4.6+/-0.7+/-0.6) x 10(-5), where the first error is statistical and the second is systematic. In addition, we measure B(B0-->J/psirho(0))=(1.6+/-0.6+/-0.4) x 10(-5).     

Critical temperature and condensed fraction of Bose-Einstein condensation in an external potential

Based on Bose-Einstein condensation at minimized momentum state, we get the expressions for the critical temperature and condensed fraction of Bose-Einstein condensation (BEC) in an external potential in the three-dimensional (3D) case. For the 1D and 2D cases, we present not only the critical temperature and corresponding particles but also the condition of BEC occurrence.     

Branching fraction measurements of B+-->rho+gamma, B0-->rho0gamma, and B0-->omegagamma

We present a study of the decays B+-->rho+gamma, B0-->rho0gamma, and B0-->omegagamma. The analysis is based on data containing 347 x 10(6) BB[over ] events recorded with the BABAR detector at the PEP-II asymmetric B factory. We measure the branching fractions B(B+-->rho+)gamma)=(1.10_(-0.33)(+0.37)+/-0.09)x10(-6) and B(B0-->rho0gamma)=(0.79(-0.20)(+0.22)+/-0.06)x10(-6), and set a 90% C.L. upper limit B(B0-->omegagamma)<0.78 x 10(-6). We also measure the isospin-averaged branching fraction B(B-->(rho/omega)gamma)=(1.25(-0.24)(+0.25)+/-0.09)x10(-6), from which we determine |Vtd/Vts|=0.200(-0.020)(+0.021)+/-0.015, where the first uncertainty is experimental and the second is theoretical.     

Sudden spreading of infections in an epidemic model with a finite seed fraction

We study a simple case of the susceptible-weakened-infected-removed model in regular random graphs in a situation where an epidemic starts from a finite fraction of initially infected nodes (seeds). Previous studies have shown that, assuming a single seed, this model exhibits a kind of discontinuous transition at a certain value of infection rate. Performing Monte Carlo simulations and evaluating approximate master equations, we find that the present model has two critical infection rates for the case with a finite seed fraction. At the first critical rate the system shows a percolation transition of clusters composed of removed nodes, and at the second critical rate, which is larger than the first one, a giant cluster suddenly grows and the order parameter jumps even though it has been already rising. Numerical evaluation of the master equations shows that such sudden epidemic spreading does occur if the degree of the underlying network is large and the seed fraction is small.     

Measurement of the CP asymmetry and branching fraction of B0-->rho0K0

We present a measurement of the branching fraction and time-dependent CP asymmetry of B(0)-->rho0K0. The results are obtained from a data sample of 227x10(6) Upsilon(4S)-->BB decays collected with the BABAR detector at the PEP-II asymmetric-energy B factory at Stanford Linear Accelerator Center. From a time-dependent maximum likelihood fit yielding 111+/-19 signal events, we find B(B(0)-->rho0K0)=(4.9+/-0.8+/-0.9)x10(-6), where the first error is statistical and the second systematic. We report the measurement of the CP parameters S(rho)(0)K(0)S=0.20+/-0.52+/-0.24 and C(rho)(0)K(0)S=0.64+/-0.41+/-0.20.     

Measurement of the branching fraction and CP asymmetry in B+ --> rho+pi0

We report a measurement of the branching fraction for the decay B+ --> rho(+) pi(0) based on a 140 fb(-1) data sample collected with the Belle detector at the KEKB asymmetric e(+)e(-) collider. We measure the branching fraction B(B(+) --> rho(+)pi(0)) = (13.2 +/- 2.3(stat)(+1.4)(-1.9)(syst)) x 10(-6), and the CP-violating asymmetry A(CP)(B-/+ -->rho(-/+)pi(0))=0.06 +/- 0.17(stat)(+0.04)(-0.05)(syst).     

Spike-burst synchronization in an ensemble of electrically coupled discrete model neurons

In this paper, we study the dynamics of a system of two model neurons interacting via the electrical synapse. Each neuron is described by a two-dimensional discontinuous map. A chaotic relaxational-type attractor, which corresponds to the spiking-bursting chaotic oscillations of neurons is shown to exist in a four-dimensional phase space. It is found that the dynamical mechanism of formation of chaotic bursts is based on a new phenomenon of generation of transient chaotic oscillations. It is demonstrated that transition from the chaotic-burst generation to the state of relative rest occurs with a certain time delay. A new characteristic which estimates the degree of synchronization of the spiking-bursting oscillations is introduced. The dependence of the synchronization degree on the strength of coupling of the ensemble elements is studied.     

The role of the cytoskeleton in cell body enlargement,increased nuclear eccentricity and chromatolysis in axotomized spinal motor neurons

Background  

When spinal motor axons are injured, the nucleolus, nucleus and cell body of the injured cell transiently increase in size, the nucleus becomes more eccentrically placed, and the organization of polyribosomes into Nissl bodies is temporarily disrupted. The mechanisms for these classical morphological responses to axotomy have not been satisfactorily explained.     

Measurements of the branching fraction and polarization in B+ --> rho+ K*0 decays

We present the results of a study of the charmless vector-vector decay B+ --> rho+ K*0, based on 253 fb(-1) of data collected with the Belle detector at the KEKB asymmetric-energy e+ e- collider. We obtain the branching fraction B(B+ --> rho+ K*0) = [8.9 +/- 1.7(stat) +/- 1.2(syst)] x 10(-6). We also perform a helicity analysis of the rho and K* vector mesons, and obtain the longitudinal polarization fraction f(L)(B+ --> rho+ K*0) = 0.43 +/- 0.11(stat)(-0.02)(+0.05) (syst).     

Adaptive synchronization control of coupled chaotic neurons in an external electrical stimulation

In this paper we present a combined algorithm for the synchronization control of two gap junction coupled chaotic FitzHugh-Nagumo (FHN) neurons in an external electrical stimulation. The controller consists of a combination of dynamical sliding mode control and adaptive backstepping control. The combined algorithm yields an adaptive dynamical sliding mode control law which has the advantage over static sliding mode-based controllers of being chattering-free, i.e., a sufficiently smooth control input signal is generated. It is shown that the proposed control scheme can not only compensate for the system uncertainty, but also guarantee the stability of the synchronized error system. In addition, numerical simulations are also performed to demonstrate the effectiveness of the proposed adaptive controller.     

Observation of subcellular structures of neurons by an illumination mode near-field optical microscope under an optical feedback control

We propose an illumination mode near-field optical microscope operated under an optical feedback for imaging biological specimens in their natural environment. For feedback control, rapidly varying evanescent signal has been generated over the sample surface. It has been found that evanescent signal can be profitably used as a control signal by utilizing its sample feature dependent discrimination sensitivity. Neurons have been successfully observed both in air and in liquid with a resolution well beyond the diffraction limit.