共查询到3条相似文献,搜索用时 0 毫秒
1.
S. R. K. Vedula T. S. Lim P. J. Kausalya E. B. Lane G. Rajagopal W. Hunziker C. T. Lim 《Experimental Mechanics》2009,49(1):3-9
Cellular adhesion and barriers formed by intercellular adhesion proteins [tight junctions (occludin and claudins) and adherens
junction (E-cadherin)] are important in maintaining tissue homeostasis. However, disruption of these junction proteins is
associated with diseases in the organ systems such as multiple sclerosis, diarrhea, asthma, and gastro-intestinal tract carcinomas
among others. In this paper, the separation force needed to separate two cells expressing some of these proteins was measured
using the dual micropipette assay. Results show that L-fibroblasts transfected with claudin-1 and claudin-2 exhibit higher
separation force (~2.8 nN and 2.3 nN, respectively) as compared to control cells or cells transfected with occludin (~1 nN).
Furthermore, the separation force was not affected on addition of calcium chelating agent (ethylene diamine tetra acetic acid,
EDTA). The separation force was, however, significantly decreased on treating cells with the actin disrupting agent Cytochalasin-D.
These results show that the dual micropipette assay is a simple and useful experimental technique for quantifying cell–cell
adhesion. 相似文献
2.
L.W. He S.P. Yan B.Q. Li G. Zhao J.R. Chu 《International Journal of Solids and Structures》2013,50(16-17):2659-2666
For accurately predicted adhesion laws of fibrillar structures contribute to the rational design of high-performance biomimetic adhesives, an adhesion model is proposed to study the directional adhesion behavior of an extensible elastic fiber that contacts a rigid smooth surface with its side surface under the coupling effect of normal and shear forces, based on the extensible Euler Bernoulli beam theory and the surface energy concept. The deformed configuration of the fiber is obtained analytically, and on the basis of this result, the detachment mode and the normal pull-off force of the fiber for a given shear force are predicted directly. It is also found that, due to the extensibility of the fiber, there exists a maximum normal pull-off force (MNPF) when an optimal shear force is applied. The MNPF will be enhanced by increasing the axial stiffness, and reduced by increasing the bending stiffness. In addition, generating an optimal pre-tension in the adhered part of the fiber will maximize the MNPF. The derived adhesion law is expected to contribute to the optimal design and applications of single-level fibrillar adhesives. 相似文献
3.
A new elastic–plastic impact–contact model is proposed in this paper. By adopting the principle of minimum acceleration for
elastic–plastic continue at finite deformation, and with the aid of finite difference method, the proposed model is applied
in the problem of dynamic response of a clamped thin circular plate subjected to a projectile impact centrally. The impact
force history and response characteristics of the target plate is studied in detail. The theoretical predictions of the impact
force and plate deflection are in good agreements with those of LDA experimental data. Linear expressions of the maximum impact
force/transverse deflection versus impact velocity are given on the basis of the theoretical results.
The project supported by the National Natural Science Foundation of China (10532020). 相似文献