共查询到16条相似文献,搜索用时 584 毫秒
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水相识别分子印迹技术 总被引:1,自引:0,他引:1
在各种基于超分子方法的仿生识别体系中,分子印迹聚合物已经证明是一种有潜力的合成受体,受到了广泛的关注。传统的分子印迹技术通常是在有机溶剂中制备对小分子具有选择性的印迹聚合物,而在水相中制备及识别生物大分子的研究仍具有相当的挑战性。从小分子到生物大分子、从有机相到水相,反映了分子印迹技术的发展趋势。本文对最近几年分子印迹在水相制备与识别方面的最新进展进行了总结与评述,探讨了水相识别印迹聚合物的设计策略与制备方法;着重介绍了水相识别技术在固相萃取、色谱固定相、药物控释、中药有效成份提取以及生物分子识别等方面的应用;指出了提高水相识别选择性的途径并对其将来的发展进行了建议与展望。 相似文献
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分子印迹技术是制备对特定目标分子具有特异性识别能力的高分子材料的技术,所制备的高分子材料被称为分子印迹聚合物.分子印迹聚合物因具有预定性、识别性和实用性三大优点已广泛应用于分离、模拟抗体与受体、催化剂以及仿生传感器等方面和领域,显示出了广泛的应用前景.作者对分子印迹技术的发展历史、基本原理、分类、应用现状以及一些新的研究热点进行了综述. 相似文献
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分子印迹技术是一种制备具有分子识别能力的聚合物的有效技术,已经广泛应用于制备对小分子具有选择性的分子印迹聚合物,但制备能够特异性识别生物大分子--蛋白质的分子印迹聚合物的研究仍然具有挑战性。本文讨论了制备蛋白质分子印迹聚合物的难点,评述了目前印迹蛋白质的方法及各自的优缺点,展望了蛋白质印迹技术的发展趋势。 相似文献
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Alaa A. Hasseb Nourel din T. Abdel Ghani Ola R. Shehab Rasha M. El Nashar 《Current Opinion in Electrochemistry》2022
The determination of biomedical markers and pathogens using electrochemical sensors is a well-established technique in which the transducer and the recognition element are used to detect the target molecule. There is a growing interest in molecularly imprinted polymer (MIPs) applications as promising recognition elements. The use of MIPs as recognition elements in electrochemical sensors offers the advantages of being fast, low cost, and, at the same time, provides accurate and selective results compared with other commonly applied routine methods for biomedical markers and pathogen detection. Compared with other nanomaterials and aptamer-based biosensors, MIP-based sensors offered excellent selectivity for low-priced reagents to be used. The aim of the current review is to discuss the most recent applications of MIP-based electrochemical sensors (2019–2021) as promising detection devices for some important biomarkers, enzymes, and pathogens, such as viruses, bacteria, and toxins. 相似文献
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Recent advances in molecular imprinting technology: current status, challenges and highlighted applications 总被引:5,自引:0,他引:5
Molecular imprinting technology (MIT) concerns formation of selective sites in a polymer matrix with the memory of a template. Recently, molecularly imprinted polymers (MIPs) have aroused extensive attention and been widely applied in many fields, such as solid-phase extraction, chemical sensors and artificial antibodies owing to their desired selectivity, physical robustness, thermal stability, as well as low cost and easy preparation. With the rapid development of MIT as a research hotspot, it faces a number of challenges, involving biological macromolecule imprinting, heterogeneous binding sites, template leakage, incompatibility with aqueous media, low binding capacity and slow mass transfer, which restricts its applications in various aspects. This critical review briefly reviews the current status of MIT, particular emphasis on significant progresses of novel imprinting methods, some challenges and effective strategies for MIT, and highlighted applications of MIPs. Finally, some significant attempts in further developing MIT are also proposed (236 references). 相似文献
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Recent Advances in Electrochemical Sensors Based on Molecularly Imprinted Polymers and Nanomaterials
This review focuses on the recent achievement during period of 2013–2018 related to the electrochemical sensors based on molecularly imprinted polymers (MIPs) combined with nanomaterials for various kinds of applications. MIPs based electrochemical sensors have found a great interest due to their high stability, short time required for electropolymerization, and high specificity towards the target analyte. The sensitivity is considered as one of the important parameter in electrochemical sensing strategies that should be improved by the combination of highly conductive nanomaterials with selective MIPs. In general, the most employed nanomaterials are magnetic nanoparticles, gold nanoparticles (AuNPs), carbon nanotubes and graphene. This review discusses the main current achievement as well as the current challenges regarding the development of biomimetic sensors in electroanalysis. 相似文献
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Molecularly imprinted polymers (MIPs) are tailor-made synthetic materials capable of selectively rebinding a target analyte, or a group of structurally related compounds based on a combination of recognition mechanisms including size, shape, and functionality. Among the advantageous properties of MIPs are the achievable specific affinity, the relative ease of preparation, and their mechanical and chemical robustness, which renders them ideal materials for applications as stationary phase (e. g., affinity chromatography or SPE), or as antibody mimics (e. g., biomimetic assays). Here, we review recent advancements on the application of MIPs in affinity separations and biomimetic assays, which have focused on the synthesis of size- and shape-uniform particles facilitating reproducibility, improved binding site accessibility, and enhanced affinity. While MIPs certainly offer promising potential as selective separation phase in a variety of applications, deeper understanding of the fundamental interactions governing imprinting, and rational understanding of the imprinting mechanism has yet to be achieved for providing rational guidelines in deliberately designing next-generation MIP materials. 相似文献
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Bisphenol A (BPA) is a well-known endocrine-disrupting industrial compound that is found throughout many aspects of our daily life; from the water we drink and the food we eat to the babies’ bottles and children’s plastic toys. Chronic exposure to BPA may result in some severe medical issues which account for the great importance of its monitoring and removal from everyday products. The use of molecularly imprinted polymers (MIPs) for that purpose has acquired a lot of traction in recent decades. MIPs are artificial antibodies with selective recognition cavities for specifically targeted substances. They are created using a variety of synthetic methods and employed in numerous types of sensors to be used in a wide range of applications. In this review, we focus on the different production methods of MIPs and the varied types of electrochemical and optical sensors that employed MIPs to detect and analyze BPA. Finally, the broad variety of applications of MIPs in environmental, foodstuff, and biological samples are thoroughly examined. Future expected trends and prospective developments are also assessed. 相似文献