共查询到19条相似文献,搜索用时 57 毫秒
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肝素亲和层析是利用生物大分子与层析介质表面的肝素特异性结合而进行选择性分离的一种技术,使用条件温和,纯化过程中能较好保持目标蛋白的生物活性。本文在阐述肝素与层析介质化学反应机理的基础上,系统论述了溴化氰活化及偶联法、环氧活化及偶联法、席夫碱法和碳二亚胺缩合法四种肝素亲和层析介质制备方法,深入分析了各制备方法的优缺点,总结了肝素亲和层析在血液制品、病毒纯化方面的应用。最后,进一步分析了肝素亲和层析在蛋白分离纯化领域应用存在的问题,并展望了其未来发展趋势,以期为肝素亲和层析介质的制备方法提供新思路。 相似文献
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甲基化聚胺型硅基强阴离子交换剂用于生物大分子的分离 总被引:1,自引:0,他引:1
用聚胺键合法合成了聚胺型碱性硅胶基质,进一步甲基化后制得了强阴离子交换剂,该强阴离子交换剂将硅工礅子交换剂和树脂型离子交换剂的优点有机地结合起来,具有稳定性好,使用PH值范围宽(PH1-12),柱渗透性好和优点,可理想地用于生物大分子和蛋白南,多肽和酸等的分离。型碱性硅胶 相似文献
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疏水膜色谱法对生物大分子的快速纯化 总被引:2,自引:0,他引:2
首次采用自制的分别级合了辛基、丁基、苯基及聚乙二醇-4000的4种常用疏水基团的纤维素疏水膜色谱柱,以键合了辛基、苯基的SepharoseCL-4B凝胶柱为对照,考察了疏水膜色谱柱对牛血清白蛋白(BSA)的动态吸附容量及流速对吸附容量的影响。疏水膜色谱柱对蛋白及酶具有较好的疏水吸附及纯化作用,但吸附容量比相应的琼脂糖凝胶柱低得多。增大流速及降低蛋白溶液质量浓度对疏水膜色谱柱的吸附容量影响较小,这些性能使膜色谱柱非常适合于大体积低质量浓度蛋白溶液(如基团工程培养液)的分离纯化。 相似文献
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壳聚糖涂层亲和层析介质合成的研究 总被引:1,自引:0,他引:1
研究了壳聚糖涂层亲和层析介质的制备工艺。采用合适的涂层条件,壳聚糖在玻璃珠上一次涂层量可达34mg/g介质。采用二氯化物交联剂可提高壳聚糖涂层介质的稳定性。当pH>4.3时,交联壳聚糖涂层介质对Cu2+的吸附容量达13.6mgCu2+/g壳聚糖。在本介质的膨胀床中,最大液体流速可达1m/h。 相似文献
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1NTRODUCTIONImmobilizedmetalionaffinitychromatography(IMAC)hasattractedconsiderableattentioninthepurificationofproteinssincePorathfirstlyuseditin1975Llj.IMAChassomeadvantagescomparingtootheraffinitychromatography.Forexample,IMACcanbeoperatedatanhighionstrengthandtheligandsuchasC.2 ischeapandstable.TheconventionalIMACadsorbentswereproducedbyreactingiminodiaceticacid(IDA)withpolysaccharidesuchasagaroseandthentheaffinityligandC.2 waschelatedtothe.d...b..t[2].Thepreparationwasexpensi… 相似文献
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Immobilized triazine dye affinity chromatography has been widely used for protein purification. In this paper, cibacron Blue F3G-A was immobilized,through a spacer arm, onto a rigid hydrophilic porous polymer by reacting an epoxy-group-containing poly(vinyl alcohol) with 6-aminohexyl-N'-Cibacron Blue F3G-A,which was obtained by reacting Cibacron Blue F3G-A with excess of 1,6-diaminohexane, in a pH 8.6 buffer. The epoxy-group-containing poly(vinyl alcohol) was prepared by hydrolysis of macroporous crosslinded poly(vinyl acetate),which was synthesized by suspension copolymerization of vinyl acetate and triallyl isocyanurate in the presence of butyl acetate and n-heptane as diluents. The cibacron Blue F3G-A-immobilized poly(vinyl alcohol)was packed in a stainless steel column (250×5 mm I. D.) and the chromatographic behaviors of several proteins (cytochrome c, lysozyme, bovine serum albumin, insulin, and lactate dehydrogenase) were determined. 相似文献
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A review on the principles and applications of boronic acids as affinity ligands for the chromatographic separation of carbohydrates, nucleic acid components, glycoproteins, and other small biomolecules. The mechanisms of interactions between boronate ligands and analytes are described. Various boronate ligands and supports are discussed. Examples of the use of boronate affinity chromatography for separation of each class of analytes are presented. 相似文献
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本文介绍五个核苷和六个核苷酸混合物的反相柱层析。柱上用涂有N263固定相(它与聚三氟氯乙烯担体的重量比为1:1)的交换剂装填。以含有KCl的咪唑·盐酸缓冲液(pH7.0)分三级洗脱,除了3'-T_p和3'-A_p外,每一对洗脱成份的分离度都大于1。 相似文献
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