共查询到17条相似文献,搜索用时 125 毫秒
1.
左金丸及类方HPLC指纹图谱与生物热活性的“谱-效”关系研究 总被引:7,自引:0,他引:7
HPLC法建立左金丸及类方水提液的特征指纹图谱, 计算各特征峰的相对峰面积, 并采用中药色谱指纹图谱相似度评价系统A版(2004 A)计算类方间的相似度; 微量热法测定大肠杆菌在左金丸及类方水提液作用下的热谱曲线, 得到相应的热动力学参数, 如生长速率常数k, 最大产热功率Pm, 最高峰的出峰时间tm和总产热量Qt等, 并对这些热动力学参数进行主成份分析. 典型相关分析法对左金丸及类方HPLC 指纹图谱中特征峰的相对峰面积与其类方作用下大肠杆菌生长代谢的主要热动力学参数相关联, 研究“谱-效”相关性. 结果表明, HPLC 指纹图谱与生物热活性存在很好的相关性, 类方中吴茱萸次碱、盐酸巴马汀、盐酸小檗碱的含量差异是导致其生物热活性不同的主要原因. 相似文献
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用微热量计对亚甲基蓝与溴酸根在水中的氧化还原反应进行了热动力学研究.根据在微量热计上测得的数据和算得该反应的热力学参数(活化焓、活化熵及活化自由能)、数率常数和动力学参数(活化能、指前因子及反应级数),对此反应的机理也作了探讨. 相似文献
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β-环糊精和十二烷基硫酸钠包合作用的微量热法研究 总被引:5,自引:0,他引:5
环糊精(CD)和客体分子的选择性包合作用是现代化学研究中的一个重要课题,化学和药剂学应用中的分离分析、稳定作用、增溶作用及利用分子识别释放活性物质等都和它有着密切的关系.β-CD和十二烷基硫酸钠(SDS)在毛细管电泳中有着广泛的应用[1],β-CD+... 相似文献
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微量热法研究黄嘌呤氧化酶反应 总被引:2,自引:0,他引:2
在热导工热量计双参数理论模型的基础上,建立了较快酶仲反应研究的双参数初始速度法的热动力学模型,用微量热法研究了黄嘌呤氧化酶催化氧化黄嘌呤的热动力学,该较快酶促反应遵循Michaelis-Menten这,在298.15K和PH=7.5时,其米氏常数为1.04×10^-3mol.L^-1,与文献结果相符。 相似文献
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微量热法研究超氧化物歧化酶反应 总被引:2,自引:0,他引:2
用微量热法研究了以过氧化氢酶反应为氧产生体系、以邻苯三酚自氧化反应为底物产生体系的超氧化物歧化酶 (SOD)催化超氧阴离子歧化反应的热动力学 ,测得 2 98 1 5K和pH 8 0时SOD反应和邻苯三酚自氧化反应的摩尔反应焓分别为- 1 6 0 .1和 - 2 1 8kJ·mol-1,并建立了一种新的SOD活力测定方法———微量热测活法 .实验结果表明 ,SOD对邻苯三酚自氧化反应的动力学参数及反应机理没有影响 ,在无SOD存在和有SOD存在时该自氧化反应在限量氧气条件下均遵循二级反应动力学 (对邻苯三酚和氧气各为一级 ) ,2 98 1 5K和 pH 8 0时其二级速率常数分别为 1 2 5和 1 30L·mol-1·s-1,同时提出了SOD抑制邻苯三酚自氧化反应的可能机理 . 相似文献
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采用LKB-2277生物活性检测系统, 测定了37 ℃时白色念珠菌在巴马汀作用下生长代谢的热谱曲线, 并获得9个相应的定量热动力学参数, 经主成分分析综合评价巴马汀的抗白色念珠菌作用. 结果表明巴马汀的抗白色念珠菌作用主要受热谱曲线第二指数生长期的生长速率常数k2和最大产热功率 的影响, 通过分析这两个主要参数的数值变化, 能更方便、快捷和准确地评价巴马汀的抗白色念珠菌作用, 为进一步评价其它药物和化合物的抗菌作用提供了有用的方法和基础. 相似文献
8.
微量热法研究黄连及其主要组分配伍的抑菌作用 总被引:4,自引:0,他引:4
基于微量热法,研究黄连、黄连的主要组分小檗碱、药根碱、巴马汀及其配伍模拟方的抑菌作用.以HPLC法测定黄连中小檗碱、药根碱和巴马汀的含量,并根据其含量比值配伍模拟方;微量热法测定黄连、小檗碱、药根碱、巴马汀及其模拟方对痢疾杆菌的生长代谢曲线,得出相应的热动力学参数,并进行对应分析.结果表明黄连、小檗碱、药根碱、巴马汀及其模拟方对痢疾杆菌的生长代谢均有不同程度的抑制作用,黄连作用最强,单体生物碱作用弱,配伍模拟方作用增强,但并未显现明显协同作用,黄连的抑菌作用可能为多种活性成分的综合作用. 相似文献
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微量热法研究PCMB对精氨酸酶的抑制作用 总被引:2,自引:2,他引:2
利用微量热法研究了对-氯汞苯甲酸(PCMB)对精氨酸的酶促水解反应的抑制作用,确定它于竞争性不可逆抑制剂,在298.15K和PH为9.4时PCMB与精氨酸酶作用的二级速度常数k+0=92.17L/(mol.s)。同时用PCMB作为修饰剂探讨了精氨酸酶的活性中心性质,推测该水解酶至多含有3个与酶活性有关的半胱氨酸残基,但主些残基不属于精氨酸酶的活性中心。 相似文献
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叶琳化合物以其对肿瘤组织的特殊亲和性和光动力学效应受到广泛的重视,国内外研究报导甚多·自D79年nel等人山证实水溶性四一K甲基毗陡基)叶咐及其金属配合物能嵌入**A的碱基之间后,人们以这类水溶性叶琳为模型利用各种物理和化学手段研究它们与*NA相互作用[2,3].但用微量热法进行研究尚未见报导.我们曾报导用共振拉曼光谱研究图1所示的二个水溶性金属叶琳ru(*A仰)」和卜i(N**刊I同**A的作用*‘],本文进一步报导用微量热法和紫外可见光谱研究的结果.1实验部分1.1试剂将小牛胸腺DN以华美生物工程公司产品)直接… 相似文献
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A microcalorimetric technique based on the metabolic heat-output was explored to evaluate the inhibition of cyanide on the mitochondrial metabolism of aquatic animal, Cyprinus carpio. The power-time curves could be divided into four parts: lag phase, active recovery phase, stationary phase, and decline phase, and the corresponding thermokinetic parameters were obtained. The maximum heat production rate Pmax decreased in a linear manner with the increase of concentration of cyanide, however, such mitochondria of aquatic animal were still metabolized actively even under the action of high concentration of cyanide. All the observations suggested that the mitochondria of this aquatic animal should exhibit considerable ability of cyanide-resistant respiration. 相似文献
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SrMoO4 nanoplates were synthesized by a facile reverse microemulsion method at room temperature. Energy evolution of this in situ growth process was monitored by means of a microcalorimeter. A sharp exothermic peak for the initial reaction and two discontinuous relatively weak exothermic peaks for the subsequent crystal growth emerged on the microcalorimetric heat flow curve. Based on the in situ thermokinetic data, the rate constants of the nucleation process and crystallization process at 298.15 K were calculated to be 4.078×10-3 and 5.033×10-4 s-1, respectively. The growth mechanism and energy evolution were investigated. 相似文献
13.
Yan Fang Shengli L. Gao Sanping P. Chen Rongzu Z. Hu Qizhen Z. Shi 《Monatshefte für Chemie / Chemical Monthly》2003,134(7):983-989
Summary. The crystal growth process of aqua methioninezinc (II) sulfate (Zn(Met)SO4·H2O) from water and acetone was investigated using a Calvet microcalorimeter. The heat produced and the rate of heat production
during the crystal growth process at 293.15, 295.15, 298.15, and 300.15 K were measured. On the basis of these results the
rate constant, the Eyring parameters (the apparent activation enthalpy, the activation entropy, and the activation free energies), and the Arrhenius parameters (the activation energy, the pre-exponential factor) of the crystal growth process have been obtained. The results
have shown that this crystal growth process accords with the Burton-Cabrera-Frank dislocation theory.
Corresponding author. E-mail: weizhang@nwu.edu.cn
Received August 16, 2002; accepted (revised) December 3, 2002
Published online May 15, 2003 相似文献
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The microcalorimetric method was used to study the antibacterial activity of two newly synthesized Schiff basecompounds(H_2L~(3')and H_2L~3)on Escherichia coli,trying to obtain the action on both of multiplying bacteria andnon-multiplying bacteria at one experiment.The metabolic power-time curves of the bacteria treated with the com-pounds were obtained,and the thermokinetic parameters were analyzed,from which the antibacterial activities ofthese compounds were evaluated.The results showed that both of the two compounds have good activity on aerobicmultiplying metabolism of E.coli,with the value of IC_(50)75.8 and 168.8 mg/L respectively,but have not effectiveaction on fermentation metabolism of E.coli.The action of the compounds on the non-multiplying metabolism wasinvestigated by taking the heat output of E.coli in the stationary phase as the guideline of the activity.The value ofMSC_(50)(minimum stationary-cidal concentration 50)of them is 118 and 187.5 mg/L,respectively.So,H_2L~(3')hasstronger antibacterial action on E.coli than H_2L~3 either for multiplying bacteria or non-multiplying bacteria,andtheir activity on the aerobic multiplying bacteria of E.coli is mainly shown.It does strongly suggest that the calo-rimetric method should play an important role in the fight against the drug-resistant bacteria. 相似文献
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The inhibitory effects of four organic acids (OAs) in Radix Isatidis, a traditional Chinese medicinal herb, on Escherichia coli (E. coli) growth were investigated by microcalorimetry. The power‐time curves of E. coli growth with and without OAs were acquired, meanwhile the extent and duration of inhibitory effects on the metabolism were evaluated by growth rate constants (k1, k2), half inhibitory ratio (IC50), maximum heat output (Pmax) and peak time (tp). The values of k1 and k2 of E. coli growth in the presence of the four OAs decreased with the increasing concentrations of OAs. Moreover, Pmax was reduced and the value of tp increased with increasing concentrations of the four drugs. The sequence of anti‐microbial activity of the four OAs was: syringic acid>2‐amino‐benzoic acid>salicylic acid>benzoic acid. IC50 of the four OAs was respectively 56 µg/mL for syringic acid, 75 µg/mL for 2‐amino‐benzoic acid, 86 µg/mL for salicylic acid and 224 µg/mL for benzoic acid. The existence of the functional groups on phenyl ring improves the anti‐microbial activity compared to benzoic acid. The functional groups methoxyl at C(3) and C(5) improve anti‐microbial activity more strongly than the other functional groups, and the functional group amino at C(2) improve anti‐microbial activity more strongly than hydroxyl at C(2) on phenyl ring. 相似文献
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The microcalorimetric method was used to study the antibacterial activity of two newly synthesized Schiff base compounds (H2L3' and H2L3) on Escherichia coli, trying to obtain the action on both of multiplying bacteria and non-multiplying bacteria at one experiment. The metabolic power-time curves of the bacteria treated with the compounds were obtained, and the thermokinetic parameters were analyzed, from which the antibacterial activities of these compounds were evaluated. The results showed that both of the two compounds have good activity on aerobic multiplying metabolism of E. coli, with the value of ICso 75.8 and 168.8 mg/L respectively, but have not effective action on fermentation metabolism of E. coli. The action of the compounds on the non-multiplying metabolism was investigated by taking the heat output of E. coli in the stationary phase as the guideline of the activity. The value of MSCso (minimum stationary-cidal concentration 50) of them is 118 and 187.5 mg/L, respectively. So, H2L^3 has stronger antibacterial action on E. coli than H2L^3 either for multiplying bacteria or non-multiplying bacteria, and their activity on the aerobic multiplying bacteria of E. coil is mainly shown. It does strongly suggest that the calorimetric method should play an important role in the fight against the drug-resistant bacteria. 相似文献
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用微量热法研究漆酶和3,4-二羟基苯甲醛的反应望天志*吴鼎泉万洪文**屈松生(武汉大学化学系,武汉430072,**华中师范大学化学系武汉)关键词漆树漆酶,微量热法,3,4-二羟基苯甲醛1996-08-08收稿,1996-10-17修回国家自然科学基... 相似文献