Alkaloids ofFumaria parviflora

The total alkaloids of the plantFumaria parviflora Lam., collected in Ashkhabad Province (Turkmen SSR), in the flowering and incipient fruit-bearing period have been studied. The methanolic extraction of 10.5 kg of the plant yielded 0.39% of total alkaloids, from which cheilanthifoline, scoulerine, parfumine, norjusiphine, isoboldine, coclaurine, dihydrosanguinarine, stylopine, sanguinarine, oxysanguinarine, adlumine, adlumidine, hydrastine, bicuculline, protopine, cryptopine, adlumidiceine, N-methyladlumine, a base with mp 281–282°C, and a new alkaloid — d-fumaricine — have been isolated from the plant for the first time.     

Alkaloids ofCorydalis paniculigera

The alkaloid composition of the roots ofCorydalis paniculigera Rgl., collected in the flowering phase in the Alai range, has been studied. Chloroform extraction yielded 0.39% of total alkaloids, from which were isolated wilsonirine, thalicmidine, coclaurine, stylopine, dihydrosanguinarine, sanguinarine, oxosanguinarine, adlumine, adlumidine, bicucculine, sibiricine, protopine, pancorine, and corunnine, and new alkaloids which have been called pancoridine (I) and pancorinine (II). The structures of (I) and (II) have been established on the basis of spectral characteristics and also the production of wilsonirine on their reduction in sulfuric acid.     

Alkaloids ofCorydalis stricta

The alkaloids of the epigeal part and roots ofCorydalis stricta have been studied. Of the 23 alkaloids isolated, N-methylcorypalline proved to be new, and its structure has been determined. Stilopine hydroxymethylate and pycnarrhine have been isolated from the genusCorydalis for the first time, and cheilanthiofoline isocorypalmine, scoulerine, isoboldine, reticuline, N-methylcoclaurine, coreximine, juziphine, pancorydine, corypalline, wilsonirine, stilopine, adlumidine, dihydrosanguinarine, adlumine, and bicucculine have been isolated from this species of plant for the first time.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 490–493, July–August, 1983.     

Alkaloids ofCorydalis gortschakovii

Summary Isocorydine, corydine, protopine,l-adlumine, d-bicuculline,l-adlumidine, gortschakoine, juziphine, domesticine, isoboldine, corytuberine, stylopine, sendaverine, and the new alkaloids sendaverine N-oxide and juziphine N-oxide have been isolated from the epigeal part ofCorydalis gortschakovii growing in the upper regions of the R. Pskem.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 834–836, November–December, 1977.     

Alkaloids ofGlaucium fimbrilligerum

From the epigeal part and roots ofGlaucium fimbrilligerum 22 alkaloids have been isolated, of which three have proved to be new — d-isocorytuberine, dehydrocorydine, and corydine N-oxide.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 224–228, March–April, 1980.     

Alkaloids of Papaver fugax

Papaver fugax growing on the shore of Lake Kazenoi Am in the Checheno-Ingushskii ASSR at the boundary with the Dagestan ASSR has yielded 0.39% of alkaloids. Seven alkaloids have been isolated, of which cheilanthifoline, scoulerine, and reticuline have been detected in this species for the first time, while it is the first time that d-remeroline has been found in a plant of thePapaver genus.Pyatigorsk Pharmaceutical Institute. Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 559–562, July–August, 1988.     

Alkaloids ofDelphinium tamarae

The roots ofDelphinium tamarae Kem. Nath., collected in the period of the withering of the epigeal part of the environs of the village Bakuriani (Georgian SSR) have yielded 2.02% of combined alkaloids. When the combined material was separated, methyllycaconitine, lycoctonine, and anthranoyllycoctonine were isolated, together with a new base having mp 286°C which has been called norsongoramine. The structure of norsongoramine has been shown on the basis of its spectral characteristics and conversion to songoramine.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 199–201, March–April, 1981.     

A study of the alkaloids of Thermopsis alterniflora

Summary 1. The epigeal part ofThermopsis alterniflora at the beginning of flowering contains 3.35% of combined alkaloids. The separation of the combined bases yielded cytisine, N-methylcytisine, pachycarpine, thermopsine, and the new alkaloid alteramine.2. The structure of 11-allyl-N-methylcytisine has been established for alteramine.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 169–174, March, 1971.     

Alkaloids ofPapaver arenarium

Continuing the separation of the total alkaloids of the plantPapaver arenarium M.B., collected in the region of Lake Sevan in the flowering period, we have isolated macrostomine, glycomarine, cheilanthifoline, sevanine, arenine, and two isomeric macrostomine N-oxides not previously described in the literature.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 76–79, January–February, 1984.     

Alkaloids from Papaver coreanum

The alkaloid pattern of the endemic plant Papaver coreanum Nakai (Papaveraceae) was determined for the first time. Eight alkaloids could be identified by LC/ESI-MS/MS and high-resolution mass spectrometry. Among them, protopine and allocryptopine represent the main components. Besides norsanguinarine, sanguinarine, dihydrosanguinarine, oxysanguinarine, lincangenine, and cryptopine, some other trace alkaloids were found whose structures remain unknown.     

The alkaloids of Dipthychocarpus strictus

Summary 1. A study of the dynamics of the accumulation of the alkaloids ofD. strictus according to the vegetation period showed the maximum accumulation of alkaloids in the period before budding with 0.2% in the epigeal part and 0.72% in the roots, and in the period of full fruit-bearing with 0.05–0.3% of combined alkaloids in the seeds.2. The separation of a mixture of bases of the epigeal part and of the seeds yielded seven alkaloids, six of which contained sulfur: diphthocarpamine, diphthocarpidine, diphthocarpaine, diphthocarpamidine, dipthocarpine, diphthocarpinine, and base No. 5.3. A study of the chemical properties and spectral characteristics of diphthocarpamidine has shown that it is N,N-diisopropylurea, and we are the first to have isolated it from a plant.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 762–765, November–December, 1975.     

Corumdephine — A new alkaloid fromDelphinium corumbosum

The epigeal part ofDelphinium corumbosum Rgl., collected in the flowering phase in the environs of Pokatilovka (Dzhungarian Ala-Tau) has yielded — in addition to the known alkaloids methyllycaconitine, lycoctonine, delcorine, deoxydelcorine, delcoridine, dehydrodelcorine, dephatine, browniine, dictysine, and dictysine acetonide — a new diterpene alkaloid with the composition C₂₅H₃₉NO₆ (I), which has been called corumdephine. The structure of corumdephine has been established on the basis of a passage from (I) to deoxydelcorine and spectral characteristics.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 95–98, January–February, 1985.     

Alkaloids ofRhinopetalum stenantherum. Structure of stenanzidine

Three new alkaloids have been isolated from the combined alkaloids of the epigeal part ofRhinopetalum stenantherum: stenanzidine, stenanzidinine, and stenanzamine.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 341–344, May–June, 1984.     

Alkaloids ofPapaver orientale

Summary From the combined alkaloids ofPapaver orientale we have isolated isothebaine, oripavine, thebaine, mecambridine, orientalidine, alpinigenine, protopine, bracteoline, oripavidine, and the new alkaloid isothebaidine, the structure of which has been established.Institute of the Chemistry of Plant Substances, Tashkent. Pyatigorsk Pharmaceutical Institute. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 474–475, July–August, 1978.     

二氢苯骈[c]菲啶类生物碱的制备及其抗乙肝病毒的活性

二氢苯骈[c]菲啶类生物碱是自然界中一类重要的生物活性成分,但含量很低.利用硼氢化还原方法,对博落回粗提物中的血根碱和白屈菜红碱混合物进行还原、分离,得到了二氢血根碱和二氢白屈菜红碱;并初步评价了其抗乙肝病毒活性.结果表明,利用硼氢化还原方法制备二氢苯骈[c]菲啶类生物碱的产率较高,还原产物具有一定的抗乙肝病毒活性.     

Alkaloids of Delphinium biternatum

Summary The combined alkaloids of the epigeal part and roots ofDelphinium biternatum have been investigated. From the epigeal part, in addition to the iliensine detected previously, acomonine, browniine, dehydrobrowniine, delphatine, anthranoyllycoctonine, and three new bases for which the structures of 10-dehydroiliensine, 10-benzoyliliensine, and 10-benzoylbrowniine have been established, have been isolated.From the roots have been isolated browniine, delphatine, and a new base, delbiterine. A lycoctonine skeleton has been established for delbiterine and the positions of the substituting groups have been determined.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 106–111, January–February, 1978.     

Analysis of alkaloids in Macleaya cordata (Willd.) R. Br. using high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry

A method for the analysis of alkaloids in Macleaya cordata (Willd.) R. Br. using high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI/MS) was developed. Using protopine (PRO), allocryptopine (ALL), sanguinarine (SA), and chelerythrine (CHE) as the model components, different columns for the separation and different mobile phases for the signal intensities of alkaloids in ESI/MS were investigated, respectively. The results showed that good separation and high signal intensities can be obtained on a high carbon loading (17%) reversed-phase C(18) column with 30 mM formic acid in mobile phase for the analysis of alkaloids. Under the optimal separation condition and UV detection (284 nm), linearity of the six alkaloids was obtained over concentration range from 0.05 to 100.00 microg/ml. The limit of detection (LOD) was 1.62, 1.87, 1.79, 1.76, 1.10, and 0.94 ng/ml for SA, CHE, PRO, ALL, dihydrosanguinarine (DHSA), and dihydrochelerythrine (DHCHE), respectively. The LODs with ESI/MS detection were lower three orders of magnitude than those obtained with UV detection. The proposed method could be used to control quality of the raw materials of the herb more comprehensively.     

Polybuffer separation of the alkaloids ofNitraria schoberi

Summary It has been established that the epigeal part of the plantNitraria schoberi L. in the budding stage contains 0.5% of alkaloids.By a combination of the fractionation of the mixture of alkaloids by polybuffer separation with other methods of separation, nine alkaloids have been isolated and studied.Fractions with a high pH value yielded the following strong bases: nitramine (pH 9 and 8), schoberidine and base A (pH 8), and nitrarine, isonitrarine, and nitramidine (pH 7). The following alkaloids were also isolated: nitraramine (pH 6.5 and 6) and schoberine and base B (pH 4 and the combined chloroform phases of the fractions with pH 7-2).Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 84–87, January–February, 1977.     

Alkaloids ofGlaucium fimbrilligerum. II

The alkaloids of the epigeal part and roots of the plantGlaucium fimbrilligerum have been studied. A total of 18 alkaloids has been isolated, of which epiglaufidine proved to be new, and its structure has been established. Columbamine has been isolated from the genusGlaucium for the first time, and magnoflorine and stilopine -hydroxymethylate from this species of plant for the first time.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Purkinje University, Brno, Czechoslovakia. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 493–496, July–August, 1983.     

Simultaneous determination of the content of isoquinoline alkaloids in Dicranostigma leptopodum (Maxim) Fedde and the effective fractionation of the alkaloids by high‐performance liquid chromatography with diode array detection

A simple and efficient method was developed for the simultaneous determination of eight isoquinoline alkaloids in methanol extracts of Dicranostigma leptopodum (Maxim) Fedde and the effective fractionation of the alkaloids of D. leptopodum by high‐performance liquid chromatography with diode array detection. The chromatographic conditions were optimized on a SinoChrom ODS‐BP column to obtain a good separation of the four types of alkaloid analytes, including two aporphines (isocorydine, corydine), two protopines (protopine and allocryptopine), a morphine (sinoacutine), and three quaternary protoberberine alkaloids (berberrubine, 5‐hydroxycoptisine, and berberine). The separation of these alkaloids was significantly affected by the composition of the mobile phase, and particularly by its pH value. Acetonitrile (A) and 0.2% phosphoric acid solution adjusted to pH 6.32 with triethylamine (B) were selected as the mobile phase with a gradient elution. With this method, a new quaternary protoberberine alkaloid was isolated and the two structural isomers (isocorydine and corydine) were baseline separated. The appropriate harvest period for D. leptopodum was also recommended based on our analysis. The method for the effective fraction of the alkaloids of D. leptopodum was optimized under this method with regard to the varying significant pharmacological activities of the alkaloids.