石英晶体微天平实时监测低密度脂蛋白在胆固醇修饰葡聚糖上的吸附及其动力学研究

利用石英晶体微天平实时监测低密度脂蛋白(LDL)在胆固醇修饰葡聚糖(CMD)上的吸附,并对其吸附动力学进行研究.结果表明,CMD与LDL之间的相互作用符合Langmuir吸附方程,当LDL浓度(ng/μL)为9.9,12.38和14.14时,其吸附平衡常数[K/(mol·L-1·s-1)]分别为0.0477,0.0536和0.0628,表面吸附量(ng/cm2)分别为107.6,139.6和167.9.最大吸附量达到284.8ng/cm2,吸附率为72.91%.     

Adsorption/Desorption of Low-density Lipoprotein on a Heparinized Surface of Gold Sensors

Heparin has been considered to be a potentially useful ligand for low-density lipoprotein(LDL)detection and analysis in a clinical context.In order to construct an affinity surface for preferential ads...     

Ellipsometry studies of fibronectin adsorption

The adsorption of fibronectin on a series of different surfaces was investigated with in situ ellipsometry. For silica and methylated silica, the adsorbed amount (Γ), the adsorbed layer thickness (δ_el) and the mean adsorbed layer refractive index (n_f) were obtained by a procedure involving studies of the bare substrate at two different ambient refractive indices, as well as four-zone averaging. It was found that the adsorbed amount of fibronectin was the same (1.9 ± 0.1 mg m⁻²) on both silica and methylated silica surfaces. However, the adsorbed layers formed on methylated silica were more extended and had a lower average protein concentration than those formed on silica. Furthermore, on both silica and methylated silica, an increasing adsorbed amount is achieved both by a denser packing of the fibronectin molecules and by a growth of the adsorbed layer normal to the surface. Furthermore, the adsorption of fibronectin on lipid surfaces was investigated. It was found that the adsorption of fibronectin on phosphatidic acid was quite significant (2.2 ± 0.2 mg m⁻²), while that on phosphatidylcholine, phosphatidylinositol and phosphatidylserine was much smaller (all 0.1 ± 0.05 mg m⁻²). These results are correlated to findings on the adsorption of fibrinogen on these surfaces, as well as on the opsonization of lipid-stabilized colloidal particles.     

吸附电位溶出分析法研究

本文以CysH为络合吸附剂,对Cu(Ⅱ)、Sn(Ⅳ)进行吸附电位溶出分析,获得了较高的灵敏度。着重研究了该体系的吸附性质、反应机理;进一步分析了几个体系的电位溶出行为,对吸附机理作了初步探讨。在此基础上提出了吸附电位溶出分析的必要条件,拟定了用电位溶出法测定半胱氨酸的新方法。     

Facile Synthesis and Adsorption Properties of Phosphonated Cellulose Beads for Selective Removal of Low-density Lipoprotein

Hyperlipemia, which induced by the abnormally elevated level of lipids in plasma such as cholesterol and triglyceride (TG), now has been widely recognized as a major risk factor of coronary heart disease1. Low-density lipoprotein (LDL) particles are the m…     

脱铝丝光沸石的吸附性能研究

丝光沸石是优良的吸附剂和工业生产对二甲苯的实用催化剂。脱铝是使丝光沸石改性的有效方法。Chen报道了脱铝丝光沸石对环己烷的吸附量在硅铝比为15～50范围内随硅铝比增加而减少,而在50～90范围内变化很小。张红浪等用高温水蒸气处理结合酸洗的方法脱铝,得到不同硅铝比的丝光沸石,并研究了脱铝对其结构、酸性和催化性能的影响。本     

蛋白质在羟基磷灰石界面吸附的研究

考察了酪蛋白酸钠(sodium caseinate,SC)和乳清分离蛋白(whey protein isolate,WPI)在表面性质不同的3种羟基磷灰石(hydroxyapatite,HA)颗粒上的界面吸附,分析了蛋白质的分子构型和HA颗粒的表面性质等因素对蛋白质在HA界面吸附的影响,重点讨论了SC和WPI肽链上磷酸化丝氨酸基团(phosphorylated serine residues,Ser-P)的数量和分布对吸附差异的影响.通过傅里叶变换红外光谱和表面电位分析发现SC和WPI无法被比表面积较小的HA颗粒有效吸附,但是在有效吸附面积较高的球状纳米HA和棒状微米HA上能够被吸附.Ser-P的存在使得SC在HA界面的吸附量更高、吸附能力更强.Ser-P数量和分布的不同则导致了SC中不同的蛋白组分在HA界面的竞争性吸附:β-酪蛋白在2μmHA界面始终存在优先吸附性;当纳米HA的浓度低于15 mg/mL时,纳米HA界面会优先吸附αs-酪蛋白.     

Adsorption Isotherm Studies on Titanium Carbide Powders

We have used adsorption isotherms to perform a comparative study of the substrate quality of five groups of titanium carbide powder, manufactured following different procedures. The isotherms were measured in an automated setup at 77.3 K using methane and argon as the adsorbates. We determined the specific surface area of each of the powders studied. We also determined whether or not there was evidence of steps (indicative of layer-by-layer adsorption) in each set of adsorption data. The isothermal compressibilities of the adsorbed films were determined from the data measured for each sample. Adsorption measurements were also conducted to determine the effect that heating the powders under vacuum had on the resulting substrate quality.     

计算机模拟内毒素吸附剂吸附机理的研究

应用计算机模拟的方法研究了内毒素吸附剂的吸附机理. 模拟结果显示, 以二甲胺为配体的吸附剂, 当β位存在羟基时, 此羟基可与内毒素分子间形成氢键, 并形成一个八元环的稳定结构. 此时吸附剂与内毒素之间存在静电、 氢键、 疏水相互作用和八元环的协同作用. 同时模拟了羟基位于配体不同位置的吸附剂与内毒素的相互作用. 结果表明, 静电作用为主要的相互作用力, 羟基的位置对吸附剂的吸附能力影响显著.     

血液灌流用胆红素吸附剂的吸附性能研究

胆红素是一种内源性毒素.急性肝损伤时,体内胆红素的排泄受到阻碍.如何能有效地排除高浓度的胆红素,引起了各国学者的高度重视^[1].文献^[2,3]报道,在血液净化疗法治疗高胆红素血症中,当胆红素吸附剂上含有氨基阳离子时,可以提高其对胆红素的吸附量.     

络合吸附体系吸附性的研究

本文论述了研究络合吸附体系吸附性的重要意义,较详细地讨论了各种方法,包括微分电容曲线法、交流极谱法和计时电量法等在吸附性研究和吸附量测定中的应用。     

气体超临界吸附研究进展

综述了近年来国内外学者对超临界气体在固体表面吸附的研究情况。指出了气体在临界温度以上吸附与临界温度以下吸附的不同之处;对现行的关于超临界气体的吸附理论研究进行了讨论,并在此基础上提出了研究中存在的总是及进一步的研究方向。     

椭圆偏振光谱技术的腐蚀研究应用

椭圆偏振光谱技术是一种高度灵敏、非破坏性的原位实时表征技术,可获取腐蚀过程“电极材料-介质”界面的动态变化信息,因此在腐蚀研究中得到了广泛应用. 本文结合解析椭圆偏振光谱数据所采用的光学模型的发展,介绍了近年来椭圆偏振光谱技术在腐蚀研究中的典型应用,并分析了该技术的发展趋势.     

Physicochemical Studies on the Adsorption Properties of Asbestos

Toxic asbestos fibers are known to be carriers of carcinogenic polyaromatic hydrocarbons (PAH) when ingested by human lungs. To clarify the asbestos-PAH interactions, the adsorption properties of four different asbestos, namely amosite, anthofillite, crocidolite, and chrysotile (termed AMOS, ANTHO, CROX, and CHRYS, respectively), toward pyrene (py) were investigated by electron paramagnetic resonance (EPR) and fluorescence spectroscopy, both steady state and time resolved. Pyrene was labeled with a nitroxide TEMPO radical (py-T) for the EPR study. EPR provided information on the adsorption ability of the surface toward py-T and on the interactions between py-T and surface sites, mainly cooperative in nature. Pyrene molecules organize on AMOS samples in a packed layer; the radical works as a separator and prevents the attaching of the py-T molecules at the surface, but the N-O groups of the few adsorbed molecules directly interact with the surface. The interacting sites at the ANTHO surface are quite far from each other; therefore py-T instead of py may form a monolayer at the asbestos surface. The surface of CROX strongly adsorbs both excimers of py and monomers of py-T, orienting the nitroxide groups toward the polar sites at the surface. The surface of CHRYS is the most adsorbing toward py and py-T, but the nitroxide group does not directly interact with the surface. By increasing concentration, py-T saturates the CROX and the CHRYS surface interacting sites, and then forms a disordered liquid-like multilayer. The close average distance calculated for py-T adsorbed at the CROX surface (12 ?) suggests that the py-T molecules intercalate in an "up-and-down" conformation. The chemical composition of the different asbestos and their different structural properties affect the distribution of the polar sites at the external surface, modifying the adsorption properties. This study confirms the synergetic effect between the toxicity of the asbestos fibers and the adsorption of polyaromatic hydro carbons at their surface, which mainly depends on the surface properties of the asbestos. Copyright 2001 Academic Press.     

Interdiffusion in Polymer Film by Ellipsometry

The long-time approximation for the time-dependence concentration of solvent into film is derived. The interdiffusion coefficients for different temperatures and the different polystyrene molecular weights were calculated by using asymptotic formulae and time-dependence concentrations of solvent into polymer films. The apparent activation energies of interdiffusion were estimated. Copyright 1999 Academic Press.     

红外椭圆偏振光谱及其应用

阐述了红外椭圆偏振光谱的原理，技术的发展，对国外ＳＩＲＥ的应用做了介绍并给出部分实例，共收集文献２９篇。     

吸附方波伏库仑法研究 Ⅱ络合吸附体系

对络合吸附体系分别推导了前行化学反应平衡常数Ｋ１和Ｋ１两种情况的方波伏库仑法的电量方程及作了实验验证，获得判别这两种络合吸附体系的理论依据。讨论了各种因素对伏库仑曲线的影响，并由实验比较了方波伏库仑法和方波伏安法的检测限。