不同环境条件对土壤-巴戟天系统重金属分布的影响

采用AA-6800型石墨炉原子吸收光谱仪研究了土壤-巴戟天系统中铅的分布情况,包括不同地段、不同土壤类型、不同土壤pH值对土壤-巴戟天入药部位根和果实中铅的含量影响。实验结果表明,环境条件变化对土壤-巴戟天系统铅含量有一定的影响,其中土壤的pH值对土壤中的有效铅浓度及巴戟天的铅含量影响较大。     

南药巴戟天中十二种无机元素与药效关系的进一步研究

在初步研究的基础上，进一步研究了不同产地巴戟天的无机元素含量差异，通过对比各元素含量、相关系数及TE图谱，说明地道药材与非地道药材的巴戟天，其特征元素的含量存在非常明显的差异，要使大面积扩种的药材提高药效，建议根据当地土壤含Mn、Fe、Cr、Co、Ni的含量，以旋微量元素肥料的方法来补充这些元素在土壤中的不足。但在微量元素肥料配方中，应注意巴戟天吸收土壤中的元素时的协同作用和拮抗作用。     

五种中药中微量元素及重金属元素的含量分析

用微波消解-原子吸收分光光度法对巴戟天、大黄、陈皮、板蓝根、茯苓等5种中药材中的7种金属微量元素进行了含量分析。结果表明,该法的线性良好,重现性在3.3%~11%之间,精密度在2.6%以内,回收率满足定量分析要求;5种药材中铅均未超标,茯苓中镉超出国家标准;5种药材里的铜含量均有超标,其中,巴戟天超标最严重;铁在5种药材中的含量最高,其中以巴戟天含量最为丰富,达到了746.750 mg/kg,其次是锌和锰,铬的含量最少。结论:对5种药材中铜均出现超标,虽然在边缘位置,但应该予以重视;巴戟天中丰富的铁元素,可能在防止癌变发生和发展中起到一定功效。     

负载氧化铁大孔树脂及其对巴戟天多糖平衡吸附的性质

采用XRD、FTTR以及ASAP2010表面分析仪对负载氧化铁颗粒的AB-8吸附树脂进行了表征,发现负载的氧化铁主要以α-Fe2O3形式存在;负载氧化铁的AB-8树脂孔径在5～25nm之间,比表面积达到550m2/g,较原AB-8树脂略有增加.采用静态吸附实验考察了负载氧化铁颗粒的AB-8吸附树脂在不同离子强度条件下对巴戟天多糖的吸附性能.结果表明,负载氧化铁后,AB-8树脂对巴戟天多糖的吸附量有显著增加,对分子质量范围在2000u左右的巴戟天多糖有明显选择性;较高的离子强度有利于巴戟天多糖在负载氧化铁颗粒的AB-8树脂上的吸附.     

巴戟天中蒽醌类成分的提取工艺研究

本文对巴戟天中蒽醌类成分的提取工艺进行了研究,实验表明:粉碎粒度为200目;100mL乙醇回流3h;100mL乙酸乙酯溶解回流稠膏物;碳酸氢钠、氢氧化钠和碳酸钠等体积混和且用量为80mL作为提取液,氯仿萃取液体积为100mL时10g巴戟天提取工艺效果较好。     

中药材巴戟天及其水煎液微量元素的分布模式

模拟传统煎药方式,制备巴戟天水煎液。用电感耦合等离子体质谱法(ICP-MS)测定了药材及其水煎液中11种微量元素Cr、Mn、Co、Ni、Cu、Zn、As、Se、Cd、Sn、Pb含量,用火焰原子吸收光谱法(FAAS)测定了微量元素Fe含量。根据微量元素含量,得到了药材及水煎液微量元素的分布模式。结果表明,巴戟天药材样品富含人体必需元素Fe、Mn、Zn,含量分别是1086.3、393.8、15.1μg.g-1;巴戟天药材与水煎液微量元素的分布模式图清晰地显示了其微量元素的分布特征,且药材与水煎液微量元素的分布模式以及不同煎制时间下水煎液微量元素的分布模式均存在明显差异,进而表明这种微量元素分布模式对中药微量元素的研究和临床应用具有参考价值。     

不同提取方法对巴戟天中金属元素含量影响的初步探讨

采用了水提、醇提及模拟胃酸浓度的酸提法对巴戟天药材进行提取,并用火焰原子吸收分光光度法分别对其中的金属元素含量进行了测定。实验结果表明,三种提取方法中,醇提法金属溶出量最少,对于有害重金属含量较高的中药,可以在中药提取的工艺当中,利用不同的提取方法除去有害重金属。通过对三种提取方法所得的提取液中Pb、Cd的存在形态研究表明,Cd在工艺过程除去相对较容易,而Pb较困难,所以应在种植巴戟天时注意避免土壤及水源的Pb污染。     

巴戟天中一种多糖的分离与结构表征

以巴戟天的根为原料, 经热水浸提、Sevag法除蛋白、乙醇沉淀和DEAE-Sepharose CL-6B离子交换柱层析, 得到一种水溶性的巴戟天多糖(MOPI-3). 通过UV、IR、NMR、GC-MS、高碘酸氧化、Smith降解和甲基化等物理化学方法对MOPI-3的纯度、理化性质和组成结构进行表征. 结果表明, MOPI-3分子量为36061, 是一种由阿拉伯糖、半乳糖和葡萄糖组成的杂多糖, 以α-1,3-吡喃葡萄糖和α-1,4-吡喃半乳糖为主链, 平均每5个葡萄糖连接一个半乳糖, 每个重复单元具有一个支链, 支链由3个呋喃阿拉伯糖以α-1,3-键型组成, 连接在主链葡萄糖的6位碳上, MOPI-3含有乙酰基, 连接在主链半乳糖的2位碳原子上.     

氢化物发生-原子荧光光谱法测定中药材中汞和砷

用氢化物发生-原子荧光光谱法测定了产于海南省的5种中药材,即槟榔、益智、砂仁、巴戟天及沉香中汞含量及砷含量.对试样的预处理和消解方法以及仪器的工作条件作了详述,还对各种对测定有影响的因素,包括试样消解所用的酸的种类及其浓度,硼氢化钾的加入浓度,五价砷的预还原和共存金属离子的干扰等也作了研究并予以优化选择.方法的检出限分别为1.5 ng·g-1(汞)和7.0 ng·g-1(砷).在对5种药材的实样进行分析的基础上作了回收率试验,所得结果为90%～93%(汞)和90%～91%(砷).     

高效液相色谱-原子荧光光谱联用技术分析6种植物源性中药中砷的形态

建立了酸热浸提-高效液相色谱-原子荧光光谱联用技术对植物源性中药的砷形态分析的方法,同时建立了微波消解-原子荧光光谱法对植物源性中药总砷的分析方法。对砷形态分析技术的工作条件进行了优化,优化条件下,As(Ⅲ),As(Ⅴ),一甲基砷(M M A),二甲基砷(DM A)等4种砷形态的检出限在13.7~30.7μg/kg之间;形态砷标准溶液6次连续测定的相对标准偏差小于5%。方法应用于牛膝、油桂、菟丝子、巴戟天、补骨脂和大云等6种中药中的砷形态分析中,4种砷形态含量占砷总量比例80.8%~96.0%之间;牛膝样品各形态砷平均加标回收率在91.0%~104.6%之间。     

不同预处理方法对巴戟中砷限量检查的影响

采用５种不同预处理方法对巴戟药材进行处理，并用古蔡氏砷斑法检测其含量，发现不同处理方法的检测结果相差甚大，为中药砷限量检查中正确选择样品预处理方法提供了参考。     

Antiviral Activities of Officinaloside C against Herpes Simplex Virus-1

The iridoid compounds in traditional Chinese medicine play a prominent role in their antiviral effects. We previously reported the anti-inflammatory effect of new iridoids from the aerial parts of Morinda officinalis. Nevertheless, several open questions remain to explore the other biological functions of these new iridoid compounds. Herpes simplex virus-1 (HSV-1) is one of the most prevalent pathogens in human beings worldwide and due to limited therapies, mainly with the guanosine analog aciclovir (ACV) and other analogs, the search for new drugs with different modes of action and low toxicity becomes particularly urgent for public health. This study aimed to explore the anti-HSV-1 effects of iridoids from the aerial parts of Morinda officinalis. The dried aerial parts of Morinda officinalis were extracted with 95% ethanol and systematic separation and purification were then carried out by modern column chromatography methods such as silica gel column, RP-ODS column, Sephadex LH-20 gel column, and semi-preparative liquid phase, and the structure of these compounds were identified through the physical and chemical properties and a variety of spectral techniques. The obtained seven new iridoid compounds were screened for antiviral activity on HSV-1 through CCK8 and the cytopathic effect, and then the plaque reduction assay, the anti-fluorescence reporter virus strain replication, and RT-qPCR experiments were carried out to further evaluate the antiviral effect. Seven new iridoid compounds (officinaloside A–G) were identified from the aerial parts of Morinda officinalis, and officinaloside C showed anti-HSV-1 activity. Further functional experiments confirmed that officinaloside C has a significant inhibiting effect on HSV-1 virus plaque formation, viral gene, and protein expression, and fluorescent virus replication. Our findings suggest that officinaloside C has significant inhibitory effects on viral plaque formation, genome replication, and viral protein expression of HSV-1 which implies that officinaloside C exhibits viral activity and may be a promising treatment for HSV-1 infection.     

Discovery of New Iridoids as Farnesoid X Receptor Agonists from Morinda officinalis:Agonistic Potentials and Molecular Stimulation

Main observation and conclusion The investigation of Morinda officinalis led to the isolation of twelve compounds (1-12),including three new iridoid glycosides ...     

New anthraquinone and iridoid from the fruits of Morinda citrifolia

From the fruits of Morinda citrifolia L., one new anthraquinone, 5,15-O-dimethylmorindol, together with five known anthraquinones and one new iridoid, morindacin, together with two known iridoids, were isolated. Their structures were elucidated by analysis of spectroscopic data.     

New constituents from the leaves of Morinda citrifolia

A new iridoid glycoside, citrifoside (1), and a new anthraquinone, 1,5,15-trimethylmorindol (2), together with 24 known compounds, were isolated from the leaves of Morinda citrifolia. The structures of the new compounds were elucidated by spectral data. 1,5,15-Trimethylmorindol (2) did not show significant cytotoxic activity by itself but showed cytotoxicity when combined with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), while citrifoside (1) did not show any activity even with TRAIL.     

Chemical Fingerprint Analysis and Quantitative Analysis of Saccharides in Morindae Officinalis Radix by HPLC-ELSD

A method based on high performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) was developed for the quantitative analysis of three active compounds and chemical fingerprint analyses of saccharides in Morindae officinalis radix. Ten batches of Morindae officinalis radix were collected from different plantations in the Guangdong region of China and used to establish the fingerprint. The samples were separated with a COSMOIL Sugar-D column (4.6 mm × 250 mm, 5 μm) by using gradient elution with water (A) and acetonitrile (B). In addition, Trapped-Ion-Mobility (tims) Time-Of-Flight (tims TOF) was used to identify saccharides of Morindae officinalis radix. Fingerprint chromatogram presented 26 common characteristic peaks in the roots of Morinda officinalis How, and the similarities were more than 0.926. In quantitative analysis, the three compounds showed good regression (r = 0.9995–0.9998) within the test ranges, and the recoveries of the method were in the range of 96.7–101.7%. The contents of sucrose, kestose and nystose in all samples were determined as 1.21–7.92%, 1.02–3.37%, and 2.38–6.55%, respectively. The developed HPLC fingerprint method is reliable and was validated for the quality control and identification of Morindae officinalis radix and can be successfully used to assess the quality of Morindae officinalis radix.     

New anthraquinones from the stem of Morinda citrifolia Linn

Studies on the chemical constituents of the stem of Morinda citrifolia, Linn. have led to the isolation of two new compounds, morindicinone (=2-hydroxy-1,8-dimethoxy-7-methoxymethylanthraquinone, 1) and morindicininone (=4-hydroxymethyl-1,3-dimethoxyanthraquinone, 2), as well as two known constituents, 2-hydroxyanthraquinone (3) and 2-methoxyanthraquinone (4). Their structures were elucidated by spectral analysis including 2D-NMR techniques.