共查询到17条相似文献,搜索用时 125 毫秒
1.
四磺基铝酞菁与爱尔新蓝的缔合作用在核酸定量中的应用 总被引:1,自引:0,他引:1
阴离子荧光染料四磺基铝酞菁与阳离子荧光染料爱尔新蓝的缔合作用 ,使四磺基铝酞菁发生荧光猝灭 ,而当核酸存在时 ,染料缔合平衡受到影响而导致四磺基铝酞菁的荧光恢复。根据这一原理 ,建立了核酸定量测定的新方法。方法具有很高的灵敏度和较好的选择性 ,其线性范围为 0~ 2 0 0 μg/L ;检测限分别为 1.8μg/L(SMDNA)、2 .0 μg/L(CTDNA)、5 .4μg/L(酵母RNA)。将方法用于实际样品金黄色葡萄球菌中DNA含量的测定 ,获得满意结果 相似文献
2.
四氨基铝酞菁作为过氧化物酶模拟酶新型红区荧光底物的研究 总被引:6,自引:0,他引:6
本文按照文献[1]合成了一种优良的红区荧光探针-四氨基铝酞菁(TAAIPc,在强酸性介质中,它的最大激发与最大发射波长分别在610nm和678nm处),并将它作为过氧化物酶模拟酶的红区荧光底物用于痕量过氧化氢的测定。由于在长波区具有荧光的天然物质很少,且溶液体系的散射光强度与1/λ^4成正比,因而在长波处进行样品测定时可以很好地避开背景荧光和散射光的干扰,检测限可得到较大的改善。 相似文献
3.
新型四羧基铝酞菁-二氧化硅荧光纳米粒子的合成及在酸度测定中的应用 总被引:3,自引:0,他引:3
首次制备出红区荧光染料四羧基铝酞菁掺杂的二氧化硅纳米粒子,并对其进行了表征。将环己烷、正己醇和表面活性剂Triton X-100按一定体积比(12.3∶1.04∶1)混合均匀,形成清澈透明的溶液;将适量的四羧基铝酞菁溶解到浓氨水中,加入到上述混合溶液中,形成反向胶束。搅拌10 m in后加入一定量的四乙氧基硅烷,加快搅拌速度,促使四乙氧基硅烷进入反相胶束中的“纳米水池”,在碱性条件下,四乙氧基硅烷水解形成二氧化硅纳米粒子。采用该方法制备的核壳荧光纳米颗粒荧光稳定性强,生物相容性高,抗干扰能力强。将一定量四羧基铝酞菁掺杂的二氧化硅纳米粒子溶于水溶液中,随溶液pH值的增加,荧光强度增强,并在pH 5.02~11.98的范围内,荧光强度与溶液酸度有良好的线性关系。该法已成功地用于自来水和模拟生物体系中pH的测定。预期该技术有望用于细胞内H 的实时监测。 相似文献
4.
甲磺基铝酞菁与爱尔新蓝的缔合作用在核酸定量中的应用 总被引:4,自引:0,他引:4
阴离子荧光染料四磺基铝酞菁与阳离子荧光染料爱尔新蓝的缔合作用,使四磺基铝酞菁发生荧光猝灭,而当核酸存在时,染料缔合平衡受到影响而导致四磺基铝酞菁的荧光恢复。根据这一原理,建立了核酸定量测定的新方法。方法具有很高的灵敏度和较好的选择性,其线性范围为0-200цg/L;检测限分别为1.8цg/L(SMDNA)、2.0цg/L(CTDNA)、5.4цg/L(酵母RNA)。将方法用于实际样品金黄色葡萄球菌中DNA含量的测定,获得满意结果。 相似文献
5.
6.
二吡啶四硝基酞菁钴的合成及光谱—电化学性质研究 总被引:1,自引:0,他引:1
本文报道了以钼酸铵为催化剂,将4—硝基邻苯二甲酸酐、脲和氯化铵混合,于220℃加热合成四硝基酞菁钴,将此配合物溶于吡啶,于115℃加热反应6天合成了二吡啶四硝基酞菁钻配合物。并对二吡啶四硝基酞菁钴的氧化还原半波电势、电解氧化还原产物的电子吸收光谱进行了研究。 相似文献
7.
8.
分子光谱法研究铝酞菁与牛血红蛋白的相互作用 总被引:5,自引:0,他引:5
利用紫外可见吸收光谱和荧光光谱研究了在生理pH条件下铝酞菁与牛血红蛋白(BHb)的相互作用.实验结果表明:铝酞菁分子与BHb发生反应生成基态复合物,导致BHb内源荧光的猝灭,该猝灭属于静态猝灭.测定了不同温度下该反应的表观结合常数、结合位点数及结合热力学参数,热力学参数的变化表明铝酞菁与BHb之间以静电和疏水作用力为主;根据Frster能量转移理论,测得供体与受体间结合距离r和能量转移效率E;并用同步荧光光谱法探讨了铝酞菁对BHb构象的影响. 相似文献
9.
10.
在弱酸性介质中,具有红区发射特性的强荧光化合物阳离子铝酞菁(TTMAAlPc)在带磺酸基团的低浓度阴离子黏多糖(肝素,HP)的存在下,发生诱导聚集,导致酞菁荧光几乎完全猝灭。此聚集缔合物可作为溶菌酶的荧光底物,在溶菌酶的水解作用下,HP降解为小分子片段,破坏了TTMAAlPc的诱导聚集行为而使其释放,体系荧光显著恢复。据此现象,建立了测定溶菌酶的新方法。结合荧光光谱与荧光各向异性技术对反应机理进行了探讨。确定了最佳反应条件(醋酸缓冲体系,pH4.0、反应温度70$C、反应时间30 min),考察了共存物质的影响。在最佳条件下,方法的线性回归方程为y=#30.12121+214.65772x,r=0.99871,线性范围为0.2~2 mg/L,检出限0.015 mg/L。本研究操作简便且有较好的选择性和灵敏度。本方法用于溶菌酶实际样品的测定,并与常规的比浊法进行了比较,结果符合良好。本研究将阳离子金属酞菁荧光探针用于酶分析,开拓了酞菁荧光探针的应用范围。 相似文献
11.
This is the first report of the determination of aniline with tetra-substituted amino aluminium phthalocyanine (TAAlPc) by a fluorimetric method. In KBr-HCl solution, nitrite ion diazotizes TAAlPc, thus, the fluorescence of TAAlPc is dramatically quenched. However, there is less quenching in the presence of aniline and the recovery in fluorescence intensity is linear with the concentration of aniline. Based on this, a novel method has been developed for the determination of aniline in aqueous solutions. Under the optimal conditions, the calibration graph for aniline is from 5 to 300 ng ml−1 with a 3σ limit of detection of 1.8 ng ml−1. The relative standard deviation for nine replicate measurements of 100 ng ml−1 aniline is 1.7%. The method was applied to the analysis of water samples with satisfactory results. 相似文献
12.
This is the first report on the determination of proteins with tetra-substituted sulphonated aluminum phthalocyanine (AlS4Pc) by resonance light-scattering (RLS). At pH 3.0, the weak RLS of AlS4Pc can be enhanced by the addition of proteins. Based on this, a novel quantitative method has been developed for the determination of proteins in aqueous solutions. Under optimal conditions, the linear ranges of the calibration curves were 0.050–2.0 μg ml−1 for both human serum albumin (HSA) and human r-IgG. The detection limits were 12.7 ng ml−1 for HSA and 16.1 ng ml−1 for human r-IgG. The method has been applied to the analysis of total protein in human serum samples collected from the hospital and the results were in good agreement with those reported by the hospital, which indicates that the method presented here is not only sensitive, simple, but also reliable and suitable for practical applications. 相似文献
13.
14.
15.
Metal(II) tetranitro phthalocyanines of cobalt, nickel and copper are synthesized in pure state by a novel modified method. The complexes are characterized using elemental, electronic and IR spectral studies. Pure metal(II) tetra-amino phthalocyanines of cobalt, nickel and copper are synthesized by reducing the nitro groups of the above complexes using sodium sulphide. These complexes are also characterized by elemental, electronic, IR spectral and magnetic susceptibility measurements. The nature of the electrical conductivities of the above three metal(II) tetra-amino phthalocyanine derivatives are studied in the temperature range 303-473 K and the data are presented. Among the effect of various substituent groups on the electrical conducting property of phthalocyanine, amine group substituent on the peripheral benzene ring of the phthalocyanine molecule has been found to increase electrical conductivity to a greatest extent. These complexes showed about 105-106 times higher electrical conductivities compared to their parent phthalocyanine compounds. 相似文献
16.
Jian-li Zou 《Microchemical Journal》2007,86(1):42-47
The preparation and utilization of silica nanoparticles as a carrier of tetra-substituted carboxyl iron phthalocyanine (TCFePc, a novel mimetic peroxidase) is reported in this article. Compared with free TCFePc, the experimental results indicated that the TCFePc entrapped in silica nanoparticles exhibited an improved catalytic activity and good reusability. By using tetra-substituted carboxyl iron phthalocyanine-silica nanoparticles (TCFePc-SiO2 Nps) to catalyze the oxidation reaction of thiamine by hydrogen peroxide, a new fluorimetric method was developed for the quantitative analysis of thiamine in pharmaceutical tablets. The influences of different conditions, such as the medium acidity, the reaction time and temperature, the concentrations of reagents and foreign substances, were all investigated. Under the optimum conditions, the calibration graph for thiamine was linear over the range of 5.0 × 10− 9-1.0 × 10− 6 mol L− 1, with a detection limit of 2.0 × 10− 9 mol L− 1. The proposed method was successfully applied to the direct analysis of thiamine in two kinds of pharmaceutical tablets, and offered the advantages of simple pretreatment, rapid determination, high sensitivity and good reusability. Hence, as a carrier of the mimetic enzyme, the silica nanoparticles are effective for enzymatic reaction processes. This method is supposed to be hopeful for the determination of thiamine in other complex raw materials. 相似文献
17.
Heteroaggregation between porphyrin and phthalocyanine bearing oppositely charged substituents 总被引:2,自引:0,他引:2
MAO Hai-Fang TIAN Hong-Jian XU Hui-JunInstitute of Photographic Chemistry Chinese Academy of Science Beijing China 《中国化学》1995,13(3):223-230
Heteroaggregates composed of cationic porphyrin, meso-tetra(p-trimethylamino)-porphyrin iodide, its zinc complex and anionic phthalocyanine sodium hydroxygallium 4,4',4",4"'-tetrasulfonated phthalocyanine have been investigated by absorption and fluorescence spectroscopy. It has been found that both free base cationic porphyrin and its zinc complex can form very stable heterodimers with anionic phthalocyanine in water, methanol and dhnethylfonnamide. The stability of the aggregate depends on the polarity as well as the ligation ability of the solvent. No evidence of higher aggregates was detected. Besides axial coordination, steric hindrance which influence the relative orientation of the macrocycles are considered. 相似文献