Terpenoids from Eupatorium fortunei Turcz

Four new terpenoids, namely, rel‐(1R,2S,3R,4R,6S)‐p‐menthane‐1,2,3,6‐tetrol ( 1 ), rel‐(1R,2R,3R,4S,6S)‐p‐menthane‐1,2,3,6‐tetrol ( 2 ), 9‐hydroxythymol 3‐O‐angelate ( 3 ), and (3β,20R)‐20‐hydroxylanost‐25‐en‐3‐yl palmitate ( 4 ), together with fourteen known compounds, were isolated from the AcOEt part of the MeOH extracts of Eupatorium fortunei. In addition, two other monoterpenoids, ‘acetone thymol‐8,9‐diyl ketal’ ( 19 ) and 8‐methoxy‐9‐hydroxythymol 3‐O‐angelate ( 20 ) were also obtained which were probably artifacts but have never been reported in the literature. The structures of the new compounds, including their relative configurations, were established by an extensive study of their spectral data, especially 1D‐ and 2D‐NMR. The cytotoxic activity of the new compounds against human hepatoma (SMMC‐7721), human leukemia (HL‐60), and human hepatocyte (LO₂) cells was investigated.     

Highly Oxygenated Monoterpenes from Eupatorium fortunei

A pair of epimers of highly-oxygenated monoterpenes were isolated from the traditional Chinese medicine Eupatorium fortunei. Their structures were elucidated on the basis of the spectral analysis as （1R＊, 2S＊, 3R＊, 4R＊, 6S＊）-1, 2, 3, 6-tetrehydroxy-p-menthane （1） and （1S＊, 2S＊, 3S＊, 4R＊, 6R＊）-1, 2, 3, 6-tetrehydroxy-p-menthane （2）.     

A new furan derivative from an endophytic Aspergillus flavus of Cephalotaxus fortunei

A new furan derivative named 5-acetoxymethylfuran-3-carboxylic acid (2), together with a known furan compound, 5-hydroxymethylfuran-3-carboxylic acid (1), were isolated from the fermentation of Aspergillus flavus, endophytic fungi in Cephalotaxus fortunei. The structures of 1 and 2 were elucidated by NMR, IR, UV and MS data, as well as compared with literature data. The compounds 1 and 2 exhibited potent antibacterial activity against Staphylococcus aureus with MIC values of 31.3 and 15.6 μg/mL, respectively. The compound 2 showed moderate antioxidant activity.     

Triglyceride accumulation inhibitory effects of new chromone glycosides from Drynaria fortunei

Two new chromone glycosides, drynachromosides C (1) and D (2), along with five known chromones (3–7), were isolated from the rhizomes of Drynaria fortunei. The structures of the two new compounds were elucidated on the basis of physico-chemical property and spectroscopic data. Triglyceride (TG) accumulation inhibitory effects of the obtained chromones on 3T3-L1 cells were investigated. The results showed that 1, 2 and 5 exhibited inhibitory activity on TG accumulation. Effects of compounds 1 and 2 on mRNA expression of PPARγ, C/EBPα and aP2 in 3T3-L1 cells were also investigated.     

Anticyanobacterial phenolic constituents from the aerial parts of Eupatorium fortunei Turcz

Four thymol derivatives and two phenolic compounds were isolated from the aerial parts of Eupatorium fortunei. The new structures were elucidated to be 7,8,9-trihydroxythymol (1), and 8,10-didehydro-7,9-dihydroxythymol (2) by means of MS and NMR analysis. The known compounds were identified as 8,9,10-trihydroxythymol (3), 10-acetoxy-8,9-dihydroxythymol (4), o-coumaric acid (5) and 4-(2-hydroxyethyl)benzaldehyde (6). Compound 3 showed strongest inhibitory effect on the growth of Microcystis aeruginosa in comparison with CuSO₄.     

Three New Sesquiterpene Pyridine Alkaloids from Euonymus fortunei

Three new macrocyclic β‐dihydroagarofuran‐type sesquiterpene pyridine alkaloids, fortuneines A ( 1 ), B ( 2 ), and C ( 3 ), together with the four known alkaloids wilfornine E ( 4 ), aquifoliunine E‐I ( 5 ), euoverrine B ( 6 ), and euojaponine I ( 7 ), were isolated from the aerial parts of Euonymus fortunei. Their structures were elucidated by spectroscopic methods, including HR‐ESI‐MS, ¹H‐ and ¹³C‐NMR, DEPT, ¹H,¹H‐COSY, HSQC, HMBC, and ROESY. This is the first isolation of the above sesquiterpene pyridine alkaloids from this plant, except for compound 6 .     

佩兰挥发性化学成分的固相微萃取研究

采用固相微萃取-气相色谱-质谱法分析佩兰中挥发性化学成分,共分离出84个组分,并鉴定了67个组分,用归一化法测定其质量分数,占总挥发性组分峰面积的98.05%。主要成分是对-伞花烃(5.19%)、芳樟醇(3.72%)、β-石竹烯(12.35%)、α-律草烯(13.39%)、α-姜黄烯(2.11%)、(-)-石竹烯氧化物(8.25%)、. /-.-4-乙酰基-1-甲基环己烯(8.91%)。     

三尖杉悬浮培养细胞中hinokiol的定量分析

建立了高效液相色谱分析三尖杉悬浮培养细胞中次生代谢产物的方法,实现了次生代谢产物的分离以及hinokiol的定量分析。样品经甲醇提取后,再用氨水-氯仿萃取。采用Apollo C18色谱柱(250 mm×4.6 mm, 5 μm)进行分离,流动相为甲醇-水,梯度洗脱,柱温为30 ℃,检测波长为290 nm,流速为1 mL/min。在0.0125~0.2 g/L范围内,hinokiol的色谱峰面积与质量浓度之间具有良好的线性关系。采用该方法测定了实际样品中hinokiol的含量,并进行了3个水平的加标回收试验,其回收率为87.2%～94.7%,相对标准偏差(n=3)为0.9%~4.2%。该方法可靠、重现性好,适合对植物培养细胞中的hinokiol进行分析。     

Four New Cycloartane (=9,19‐Cyclolanostane) Saponins from the Aerial Parts of Thalictrum fortunei

The four new cycloartane (=9,19‐cyclolanostane) glycosides 1 – 4 were isolated from the aerial parts of Thalictrum fortunei (Ranunculaceae). The structures of these new glycosides were elucidated as (3β,16β,24S)‐cycloartane‐3,16,24,25,30‐pentol 3,25‐di‐β‐D ‐glucopyranoside ( 1 ), (3β,16β,24S)‐24‐(acetyloxy)cycloartane‐3,16,25,30‐tetrol 3,25‐di‐β‐D ‐glucopyranoside ( 2 ), (3β,16β,24S)‐24‐(acetyloxy)‐3‐(β‐D ‐glucopyranosyloxy)cycloartane‐16,25,30‐triol 25‐[β‐D ‐glucopyranosyl‐(1→6)‐β‐D ‐glucopyranoside] ( 3 ), and (3β,16β,24S)‐24‐(acetyloxy)‐3‐(β‐D ‐glucopyranosyloxy)cycloartane‐16,25,30‐triol 25‐[β‐D ‐glucopyranosyl‐(1→4)‐β‐D ‐glucopyranoside] ( 4 ). The structure elucidations were accomplished by 1D ‐ and 2D‐NMR methods, HR‐ESI‐MS, and hydrolysis.     

A Novel Alkaloid from Stemona parviflora

A novel alkaloid was isolated from the stems and leaves of Stemona parviflora Wright Based on the spectral methods,its structure was elucidated as parvineostemonine.     

A Novel Prenylchalcone from Humulus lupulus

From hops of Humulus lupulus, a novel prenylchalcone was isolated, which showed inhibition against BGC-823 cells. The structure was elucidated as 5“-(2“‘-hydroxyisopropyl-)-dihydrofurano-[2“, 3“-B]-4, 4‘-dihydroxy-6‘-methoxychalcone, by HRFABMS and NMR spectra.     

A Novel Triterpenoid from Petasites tricholobus

A novel triterpenoid, D:B-friedoursane-3α; 16α-dihydroxy-7α, 8α-epoxy-5(10)-ene, named petatrichol A, was isolated from the roots of Petasites tricholobus Franch.. Its structure was elucidated by spectroscopic methods, especially 2DNMR techniques.     

A Novel Ursane Triterpene from Rubus swinhoei

As a part of investigation on the plant of Rubus, we studied the chemical constituents of the roots of R. swinhoei. A novel ursane triterpene, named swinhoeic acid, was isolated and converted into its methyl-ester with CH2N2. This paper deals with its structure elucidation.Figure 1 The structure of 1 and 1a Figure 2 The key HMBC correlationsCompound 1a, was obtained as white powder, [?] - 66 (c 0.1, CHCl3). Its molecular formula was assigned as C31H48O5 by HR-EIMS ([M]+ m/z 500.3487…     

A Novel Terpenoid from Lappula anocarpa

A novel terpenoid, named lappulanocarpine A 1 was isolated from the alcoholic extract of the whole plant of Lappula anocarpa. Its structure was characterized by I D-, 2D-NMR and HR-ESIMS.     

A Novel Triterpenic Acid from Gymnema sylvestre

A novel oleanane-type triterpenic acid was isolated from the leaves of Gymnema sylvestre (Asclepiadaceae). The structure was characterized as 3β, 16β, 22β, 28-tetrahydroxy- olean-12-en30-oic acid on the basis of spectral evidence, including 1D- and 2D-NMR (HMQC, HMBC,^1H-^1H COSY and NOESY).     

A Novel Polypeptide from Cervus elaphus Linnaeus

A novel polypeptide having stimulant effect on some cell proliferation was isolated from the velvet antler (Cervus elaphus Linnaeus). The velvet antler polypeptide consists of a single chain of 32 amino acid residues. Amino acid sequence of the polypeptide was identified as:VLSAADKSNVKAAWGKVGGNAPAFGAEALLRM.     

A Novel Pregnane Glycoside from Biondia chinensis

A novel pregnane glycoside, biondianoside E, was isolated from the roots of Biondia chinensis. By the spectroscopic and chemical methods, this structure was elucidated as 3β, 5β,14β, 20S, 21-pentahydroxypregnane 3-O-β-D-glucopyranosyl-(1→4)-β-D-cymaropyranoside.     

A Novel Peptide from Buthus martensii Karch

A novel peptide was purified and characterized from Buthus martensii Karch.The peptide,named BmK M6,is a single-chain polypeptide cross-linked by four intramolecular disulfide bridges.The molecular weight of the peptide was determined by MOLDI-TOF-MS as 7034 Da.The partial amino acid sequence of BmK M6 from N-terminal is VRDAYIAKPEN CVYECGITQDCNKLCTENG.     

A Novel Cardiac Glycoside from Parepigynum funingesis

From the dried aerial part ofParepigynumfuningesis Tsiang et P. T. Li (Apocynaceae),a new cardiac glucoside, named parefuningoside (1) had been isolated. Its structure was determined by means of hydrolysis and spectral analysis.