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电化学分析的发展及应用 总被引:4,自引:0,他引:4
评述了从2001年1月-2002年12月间我国电化学分析发展的概况。内容分极谱与伏安法,微电极、超微电极和修饰电极,离子选择性电极与传感器,示波分析法,电泳及色谱电化学,光谱电化学、电致发光,扫描电化学显微镜及石英晶体微天平,化学计量学方法,电位分析及其它和仪器装置及实验技术等。引用文献591篇。 相似文献
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蛋白质的翻译后修饰对于生命体执行正常的生理功能具有十分重要的作用,是蛋白质科学研究的重要内容.目前研究蛋白质修饰的方法主要有质谱法、亲和层析等,然而由于蛋白质修饰研究的复杂性,迫切需要发掘新的技术手段.电化学方法理论成熟、应用广泛,在生命科学许多领域发挥着越来越重要的作用.蛋白质的体外修饰必将导致蛋白质特定位点基团的变化,可以利用巧妙设计的电化学方法予以表征和分析,以期探明修饰对蛋白质结构和功能的影响.此外,又可以利用电化学定量分析的独特优势快速准确地测定蛋白质修饰中涉及的相关酶活.正因为如此,蛋白质体外修饰的电化学研究已引起越来越多的关注.本文以作者课题组近期研究工作为主,结合国内外同行的相关代表性工作,介绍电化学方法在蛋白质修饰方面的近期研究进展,并探讨了今后的发展方向和趋势. 相似文献
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氧化还原蛋白质在模拟生物膜修饰电极上的直接电化学 总被引:8,自引:0,他引:8
评述了氧化还原蛋白在模拟生物膜这种新型的化学修饰电极上的直接电化学研究的进展。对蛋白质在表面活性剂薄膜电极和多层复合薄膜电极上的电化学行为、模拟生物膜的超分子结构以及蛋白质在该类薄膜修饰电极上对不同底物的电催化性质进行了较详细的介绍。 相似文献
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纳米材料修饰电极在电化学分析中的应用研究进展 总被引:1,自引:0,他引:1
综述了纳米材料修饰电极在电化学分析中的应用研究.主要总结了国内外纳米金属材料、纳米金属氧化物材料、碳纳米管与碳纳米管复合物以及其他纳米材料在电化学分析中的应用研究,并指出了纳米材料修饰电极在电化学分析应用中存在的问题. 相似文献
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蛋白质的磷酸化作用是一种可逆的翻译后修饰方式,几乎参与生物体内所有关键生命活动,在核内基因的特定表达、细胞的分裂分化以及各种生命活动的转导中发挥重要意义。基于磷酸化前后电化学信号的改变,利用电化学传感器的方法能快速简便地检测蛋白质的磷酸化作用,且具有较高的选择性和灵敏度。本文回顾了几种常用的电化学检测磷酸化作用的方法,基于电极材料的不同进行了分类与总结,汇总了每种方法中用到的修饰电极的纳米材料及生物分子,最后总结了电化学方法检测蛋白质磷酸化的优缺点,并对有效检测磷酸化作用进行了前景展望。 相似文献
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This paper reviews the recent progress in the electron transfer and interfacial behavior of redox proteins. Significant achievements in the relevant fields are summarized including the direct electron transfer between proteins and electrodes, the thermodynamic and kinetic properties, catalytic activities and activity regulation of the redox proteins. It has been demonstrated that the electrochemical technique is an effective tool for protein studies, especially for probing into the electron transfer and int... 相似文献
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This review overviews recent reports on the electroanalytical applications of boron-doped diamond (BDD) electrodes. Because BDD electrodes have excellent features for electroanalysis, such as wide potential window, low background current, electrochemical stability, and fouling resistance, they can be useful for sensitive and stable detection of various substances, including drugs, bio-related substances, metal ions, and organic pollutants. Many articles have reported high-sensitivity detection of real samples, demonstrating that this electrode material is practically applicable. Surface modification of the BDD electrodes using metal nanoparticles, nanocarbons, and polymers can increase the sensitivity of the electrochemical detection. Furthermore, research on the electroanalytical device equipped with BDD electrodes will be expanded by combining peripheral technologies related to the device fabrication. 相似文献
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A simple and effective glucose biosensor based on immobilization of glucose oxidase (GOD) in graphene (GR)/Nafion film was constructed. The results indicated that the immobilized GOD can maintain its native structure and bioactivity, and the GR/Nafion film provides a favorable microenvironment for GOD immobilization and promotes the direct electron transfer between the electrode substrate and the redox center of GOD. The electrode reaction of the immobilized GOD shows a reversible and surface‐controlled process with the large electron transfer rate constant (ks) of 3.42±0.08 s?1. Based on the oxygen consumption during the oxidation process of glucose catalyzed by the immobilized GOD, the as‐prepared GOD/GR/Nafion/GCE electrode exhibits a linear range from 0.5 to 14 mmol·L?1 with a detection limit of 0.03 mmol·L?1. Moreover, it displays a good reproducibility and long‐term stability. 相似文献
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Christine M Welch Olga Nekrassova Xuan Dai Michael E Hyde Richard G Compton 《Chemphyschem》2004,5(9):1405-1410
The tabrication, characterisation, and electroanalytical application of gold and gold amalgam nanoparticles on glassy carbon electrodes is examined. Once the deposition parameters for gold nanoparticle electrodes were optimised, the analytical utility of the electrodes was examined in CrIII electroanalysis. It was found that gold nanoparticle modified (Au-NM) electrodes possess higher sensitivity than gold macroelectrodes. In addition, gold amalgam nanoparticle modified (AuHg-NM) electrodes were fabricated and characterised. The response of those electrodes was recorded in the presence of important environmental analytes (heavy metal cations). It was found AuHg-NM electrodes demonstrate a unique voltammetric behaviour and can be applied for electroanalysis when enhanced sensitivity is crucial. 相似文献
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This review covers recent advances in the development of new designs of electrochemical sensors and biosensors that make use of electrode surfaces modification with carbon nanotubes. Applications based on carbon nanotubes-driven electrocatalytic effects, and the construction and analytical usefulness of new hybrid materials with polymers or other nanomaterials will be treated. Moreover, electrochemical detection using carbon nanotubes-modified electrodes as detecting systems in separation techniques such as high performance liquid chromatography (HPLC) or capillary electrophoresis (CE) will be also considered. Finally, the preparation of electrochemical biosensors, including enzyme electrodes, immunosensors and DNA biosensors, in which carbon nanotubes play a significant role in their sensing performance will be separately considered. 相似文献
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Vesna Vukojević Sladjana Djurdjić Sonja Jevtić Marija V. Pergal Aleksandar Marković Jelena Mutić 《International journal of environmental analytical chemistry》2013,93(13):1175-1185
ABSTRACTIn this work, the electrochemical behaviour and the subsequent development of an analytical procedure for quantification of pesticide azamethiphos, using boron-doped diamond (BDD) electrode are reported for the first time. It was found that azamethiphos electrochemical behaviour is irreversible oxidation at the potential of around 1.70 V, in 1 M nitric acid (pH 0). Also, it was found that potential of this oxidation was not pH dependent which can be attributed to the no proton involvement in electrochemical reaction on the electrode surface. The square wave voltammetric method was most appropriate for azamethiphos quantification. Under optimised experimental conditions, linear working range from 2 to 100 µM was estimated with the detection limit of 0.45 µM. Negligible effect of the possible interfering compound was observed. The obtained results show that the developed analytical methodology can be an adequate replacement for the, up to date, used methods for detection of organophosphorous pesticide. 相似文献
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Yuling Wang Rong Jin Neso Sojic Dechen Jiang Hong‐Yuan Chen 《Angewandte Chemie (International ed. in English)》2020,59(26):10416-10420
The inside walls of a nanopipette tip are decorated by a Pt deposit that is used as an open bipolar electrochemiluminescence (ECL) device to achieve intracellular wireless electroanalysis. The synergetic actions of nanopipette and of bipolar ECL lead to the spatial confinement of the voltage drop at the level of the Pt deposit, which generates ECL emission from luminol. The porous structure of Pt deposit permits the electrochemical transport of intracellular molecules into the nanopipette that is coupled with enzymatic reactions. Thus, the intracellular concentrations of hydrogen peroxide or glucose are measured in vivo as well as the intracellular sphingomyelinase activity. In comparison with the classic bipolar ECL, the remarkably low potential applied in our approach is restricted inside the nanopipette and it minimizes the potential bias of the voltage on the cellular activity. Accordingly, this wireless ECL approach provides a new direction for analysis of single living cells. 相似文献