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Investigation of the Interaction between Isoflavonoids and Bovine Serum Albumin by Fluorescence Spectroscopy 总被引:1,自引:0,他引:1
The interactions of bovine serum albumin (BSA) with three structurally related isoflavonoids, genistein, puerarin and daidzein, were studied under physiological conditions by fluorescence spectroscopic technique. The quenching mechanism of these compounds with BSA was suggested as static quenching and the binding constants were determined at different temperatures based on the fluorescence quenching results. The transfer efficiency of energy and distance between the acceptor and BSA were investigated on the basis of the mechanism of the Forster energy transference. According to the thermodynamic parameters it has been suggested that the acting force be mainly hydrophobic force. The comparison of binding potency of the three isoflavonoids to BSA showed that the substitution by 5-OH and 8-Glc could enhance the binding affinity. All these obtained in the work can make us better understand the mode of the action and pharmacological activities of the isoflavonoids. 相似文献
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荧光光谱法研究木犀草素与牛血清白蛋白的相互作用 总被引:3,自引:0,他引:3
在pH为7.40的Tris-HCl缓冲体系中,采用荧光光谱技术研究了木犀草素与牛血清白蛋白(BSA)的相互作用。根据测定292K、299K、311K温度下的猝灭常数,证实了木犀草素对BSA的荧光猝灭为静态猝灭过程,根据Frster非辐射能量转移理论计算出木犀草素与BSA间的结合距离r=2.75nm,由热力学参数焓变(△H)小于零和熵变(△S)大于零,推断出木犀草素与BSA之间主要靠静电引力相结合,生成自由能变(△G)为负值,表明木犀草素与BSA的作用过程是一个自发过程;同时,应用同步荧光光谱考察了木犀草素对BSA构象的影响。 相似文献
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TongKuanXU XingHalSHEN NaLI HongChengGAO 《中国化学快报》2005,16(7):943-946
The binding between three surface-active substituted 3H-indole fluorescence probes and bovine serum albumin (BSA) in aqueous solution was studied using fluorescence quenching. The binding constants of 3H-indole molecules with BSA were obtained. According to the Foerster resonance energy transfer theory, the distances between 3H-indole molecules and tryptophan of BSA were calculated. The results show that the oligoethyloxyethylene chain of 3H-indole molecules is longer, the binding between them is stronger, the energy transfer efficiency is higher, and the distance between tryptophan and 3H-indole is nearer. 相似文献
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采用荧光光谱法和紫外可见光谱法研究了硫唑嘌呤(AZP)和巯嘌呤(6-MP)与牛血清白蛋白(BSA)的结合反应特性.测定了不同温度下的结合常数KA及结合位点数n,研究证明AZP和6-MP对BSA内源荧光的猝灭机理均为静态猝灭,且主要以疏水作用力与BSA作用;利用同步荧光技术发现AZP和6-MP对BSA的构象均有影响;AZP和6-MP与BSA摩尔比为1:l时,根据Forster偶极-偶极非辐射能量转移理论,计算出作用距离rAZP-2.94,r6-MP-4.10,说明AZP和6-MP与BSA的猝灭过程中都存在能量转移效应. 相似文献