共查询到20条相似文献,搜索用时 15 毫秒
1.
Bouafsoun A Helali S Mebarek S Zeiller C Prigent AF Othmane A Kerkeni A Jaffrézic-Renault N Ponsonnet L 《Bioelectrochemistry (Amsterdam, Netherlands)》2007,70(2):401-407
The electrochemical impedance spectroscopy (EIS) technique has been shown to be an effective tool for monitoring endothelial cell behaviour on a multilayer functionalised gold electrode. Polystyrene, a reproducible model substrate, is deposited as a thin layer on a thiol functionalised gold electrode. Fibronectin, a protein promoting endothelial cell adhesion, is then adsorbed on the polystyrene surface. The different steps of this multilayer assembly are characterized by Faradaic impedance. The charge transfer resistance and the capacitance for the total layer are modified at each step according to the electrical properties of each layer. This gives the endothelial cells' electrical state in terms of its resistive and capacitive properties. In this study, the endothelial cell layer presents a specific charge transfer resistance equal to 1.55 kOmega cm(2) with no large defects in the cell layer, and a specific capacitance equal to few microF cm(-2) explained by the existence of pseudopods. These electrical properties are correlated to the endothelial cell viability, adhesion and cytoskeleton organization. 相似文献
2.
Ameur S Martelet C Jaffrezic-Renault N Chovelon JM 《Applied biochemistry and biotechnology》2000,89(2-3):161-170
This article deals with a direct electrochemical method of detecting antigens using new methods of functionalization of gold
electrodes. Based on the reacting ability of gold with sulfhydryl groups, three protocols for the fixation of antibodies have
been explored. They are based on either the self-assembling properties of functional thiols bearing long alkyl chains or the
possibility of a direct coupling of antibody moieties. Coverage rates as high as 97% can be reached. The analysis of the electrochemical
impedance behavior of such layers can lead to a sensitive method for the direct detection of the antibody/antigen interaction.
The addition of a redox couple in the tested solution, acting as an amplifier, allowed detection limits for the antigens as
low as a few picograms/milliliter to be reached. 相似文献
3.
Matsumura H Ortiz R Ludwig R Igarashi K Samejima M Gorton L 《Langmuir : the ACS journal of surfaces and colloids》2012,28(29):10925-10933
Achieving efficient electrochemical communication between redox enzymes and various electrode materials is one of the main challenges in bioelectrochemistry and is of great importance for developing electronic applications. Cellobiose dehydrogenase (CDH) is an extracellular flavocytochrome composed of a catalytic FAD containing dehydrogenase domain (DH(CDH)), a heme b containing cytochrome domain (CYT(CDH)), and a flexible linker region connecting the two domains. Efficient direct electron transfer (DET) of CDH from the basidiomycete Phanerochaete chrysosporium (PcCDH) covalently attached to mixed self-assembled monolayer (SAM) modified gold nanoparticle (AuNP) electrode is presented. The thiols used were as follows: 4-aminothiophenol (4-ATP), 4-mercaptobenzoic acid (4-MBA), 4-mercaptophenol (4-MP), 11-mercapto-1-undecanamine (MUNH(2)), 11-mercapto-1-undecanoic acid (MUCOOH), and 11-mercapto-1-undecanol (MUOH). A covalent linkage between PcCDH and 4-ATP or MUNH(2) in the mixed SAMs was formed using glutaraldehyde as cross-linker. The covalent immobilization and the surface coverage of PcCDH were confirmed with surface plasmon resonance (SPR). To improve current density, AuNPs were cast on the top of polycrystalline gold electrodes. For all the immobilized PcCDH modified AuNPs electrodes, cyclic voltammetry exhibited clear electrochemical responses of the CYT(CDH) with fast electron transfer (ET) rates in the absence of substrate (lactose), and the formal potential was evaluated to be +162 mV vs NHE at pH 4.50. The standard ET rate constant (k(s)) was estimated for the first time for CDH and was found to be 52.1, 59.8, 112, and 154 s(-1) for 4-ATP/4-MBA, 4-ATP/4-MP, MUNH(2)/MUCOOH, and MUNH(2)/MUOH modified electrodes, respectively. At all the mixed SAM modified AuNP electrodes, PcCDH showed DET only via the CYT(CDH). No DET communication between the DH(CDH) domain and the electrode was found. The current density for lactose oxidation was remarkably increased by introduction of the AuNPs. The 4-ATP/4-MBA modified AuNPs exhibited a current density up to 30 μA cm(-2), which is ~70 times higher than that obtained for a 4-ATP/4-MBA modified polycrystalline gold electrode. The results provide insight into fundamental electrochemical properties of CDH covalently immobilized on gold electrodes and promote further applications of CDHs for biosensors, biofuel cells, and bioelectrocatalysis. 相似文献
4.
Metal tetra-amino phthalocyanine complexes (MTAPc; where M is Co or Mn) were immobilized on screen-printed gold electrodes pre-modified with monolayers of benzylamino groups. The functionalized electrodes were then activated using benzene-1,4-dicarbaldehyde as a linker before MTAPc complexes were immobilized. The surface coverages for the modified electrodes confirmed the perpendicular orientation of the MTAPcs. The apparent electron transfer constant (kapp) for the electrodes is 2.2?×?10?5 cm.s?1 for both CoTAPc and MnTAPc modified electrodes as calculated with data from impedance measurements. The kapp values for the bare and benzylamino modified electrodes were found to be 1.2?×?10?4 cm.s?1 and 4.9?×?10?6 cm.s?1, respectively. The electrocatalysis of the modified electrodes towards detection of H2O2 gave significant peak current densities and electrocatalytic potentials at ?0.28 V and ?0.31 V for the MnTAPc and CoTAPc modified electrodes, respectively. 相似文献
5.
Valiokas R Vaitekonis S Klenkar G Trinkūnas G Liedberg B 《Langmuir : the ACS journal of surfaces and colloids》2006,22(8):3456-3460
Dip-pen nanolithography (DPN) is employed to develop a generic array platform for the selective recruitment of membrane protein complexes. An atomic force microscope tip inked with HS(CH2)16NH2 is used to generate amino-terminated domains on gold. These domains can be arranged into microscopic and submicroscopic patterns, and the untreated gold substrate is subsequently blocked with HS(CH2)2CONH(CH2CH2O)15CH3, a compound known to resist the unspecific binding of proteins and cells. The patterned gold substrate is exposed to an enriched membrane fraction from mutant Rhodobacter sphaeroides, which contains photosynthetic core complexes consisting of the reaction center and the light-harvesting complex LH1. The selective recruitment to the patterned domains, governed primarily by electrostatic interactions, is confirmed by contact mode atomic force microscopy. 相似文献
6.
The bio‐compatibility of ion implanted polymers has been studied by means of in vitro attachment measurements of bovine aorta endothelial cells. The specimens used were polystyrene (PS), polyethylene (PE), polypropylene (PP) and expanded polytetrafluoroethylene (ePTFE). He+ and Ne+ ion implantation were performed at an energy of 150 keV with fluences between 1 × 10 13 to 1 × 10 17 ions/cm 2 at room temperature. Wettability was estimated by means of a sessile drop method. The chemical and physical structures of ion implanted polymers were investigated by contact angle measurements, atomic force microscopy and X‐ray photoelectron spectroscopic analysis in relation to cell attachment behavior. The strength of cell attachment on ion implanted specimens at static and under flow conditions was also measured. Ion implanted PP and ePTFE were found to exhibit remarkably higher adhesion and spreading of endothelial cells than non‐implanted specimens. In contrast to these findings, ion implanted PS and PE only demonstrated a little improvement of cell adhesion in this assay. Copyright © 2001 John Wiley & Sons, Ltd. 相似文献
7.
Chirea M García-Morales V Manzanares JA Pereira C Gulaboski R Silva F 《The journal of physical chemistry. B》2005,109(46):21808-21817
Polyelectrolyte/gold nanoparticle multilayers composed of poly(l-lysine) (pLys) and mercaptosuccinic acid (MSA) stabilized gold nanoparticles (Au NPs) were built up using the electrostatic layer-by-layer self-assembly technique upon a gold electrode modified with a first layer of MSA. The assemblies were characterized using UV-vis absorption spectroscopy, cyclic and square-wave voltammetry, electrochemical impedance spectroscopy, and atomic force microscopy. Charge transport through the multilayer was studied experimentally as well as theoretically by using two different redox pairs [Fe(CN)(6)](3-/4-) and [Ru(NH(3))(6)](3+/2+). This paper reports a large sensitivity to the charge of the outermost layer for the permeability of these assemblies to the probe ions. With the former redox pair, dramatic changes in the impedance response were obtained for thin multilayers each time a new layer was deposited. In the latter case, the multilayer behaves as a conductor exhibiting a strikingly lower impedance response, the electric current being enhanced as more layers are added for Au NP terminated multilayers. These results are interpreted quite satisfactorily by means of a capillary membrane model that encompasses the wide variety of behaviors observed. It is concluded that nonlinear slow diffusion through defects (pinholes) in the multilayer is the governing mechanism for the [Fe(CN)(6)](3-/4-) species, whereas electron transfer through the Au NPs is the dominant mechanism in the case of the [Ru(NH(3))(6)](3+/2+) pair. 相似文献
8.
We have developed a three-step method to graft molecularly imprinted polymer (MIP) thin films onto Au electrodes. In the first step, propargyl acrylate is clicked onto an azidoundecanethiol (N(3)(CH(2))(11)SH)/decanethiol mixed self-assembled monolayer (SAM). Then, by applying UV light (365 nm) in the presence of N,N'-methylenebis(acrylamide) (MAAM) and azobisisobutyronitrile (AIBN) as the radical initiator, polymerization was carried out directly on the electrode surface in the presence of an electroactive template molecule, hydroquinone (HQ). Detection of HQ using the clicked-on MIP sensor was studied using chronoamperometry and its behavior was compared to that of a sensor prepared by drop-coating MIPs onto Au. The detection limit of the clicked-on MIP sensor for HQ was found to be 1.21±0.56 μM, about four times lower than what was observed using the coated-on MIP sensor. In addition, the sensitivity of the clicked-on MIP sensor was found to be approximately three times greater than the coated-on MIP sensor. Apparent diffusion coefficients determined using chronoamperometry suggest that the improved performance is likely due to the favorable mass transfer characteristics of the clicked-on MIP sensing membrane. 相似文献
9.
The influence of extracellular polymeric substances (EPSs) on bacterial cell electrokinetic properties and on cell adhesion onto glass beads in connection with bacterial cell electrokinetic properties was investigated using 12 heterotrophic bacterial strains. Bacterial cell surface properties such as the softness 1/lambda and charge density ZN were determined by Ohshima's soft-particle analysis using the measured electrophoretic mobility as a function of ionic strength. In 10 of 12 strains, when EPSs covering the cell surface were removed, the softness of the cell decreased, indicating that EPS adsorption enhanced the ease of liquid fluid in the ion-penetrable layer on the cell surface. On the other hand, the negative charge density of the cell surface increased for 9 of 12 strains, suggesting that EPSs covering the cell surface decreased the negative charge density of the cell surface layer. In addition, the characteristics of bacterial cell adhesion onto glass beads were evaluated by the packed-bed method and the data were interpreted to indicate cell adhesiveness. As a result, the efficiency of cell adhesion onto glass beads increased as negative cell surface potential psi0 decreased, whereas there seemed to be no correlation between zeta potential and cell adhesiveness. Cell surface potential psi0, which was derived by taking the bacterial polymer layer with EPSs into consideration, provided a more detailed understanding of the electrokinetic properties of bacterial cells. 相似文献
10.
Anamelechi CC Clermont EE Brown MA Truskey GA Reichert WM 《Langmuir : the ACS journal of surfaces and colloids》2007,23(25):12583-12588
A dual ligand (DL) system that combines high affinity streptavidin-biotin binding with lower affinity fibronectin-integrin ligand binding was developed to augment endothelial cell adhesion to polymers. In this study, we examined the utility of biotinylated fibronectin (bFN) as an enhancement to the previously developed DL approach. The goal was to make the system more amenable to clinical studies by eliminating xenogenic bovine serum albumin (bBSA). Fibronectin (FN) biotinylation was achieved with Sulfo-NHS-LC-Biotin. The affinity of conjugated biotin for wild-type streptavidin (WT-SA) and a mutant strain streptavidin (RGD-SA) was measured using surface plasmon resonance (SPR) spectroscopy. Enzyme-Linked ImmunoSorbent Assay (ELISA) absorbance values confirmed the accessibility of the cell binding domain on mildly biotinylated bFN when compared to unmodified native protein. SPR binding analysis confirmed similar binding behavior to bFN with WT-SA and RGD-SA. Kinetic analysis, however, showed no increase in affinity due to increased biotins per FN, an indication of the absence of positive cooperativity in the system. We verified the essential utility of bFN in affinity binding by SPR and confirmed the potential for integrin-FN linkages by ELISA. Finally, Vinculin immunostaining was used to determine focal adhesion formation using bFN in the DL system. Significantly greater focal adhesion density was achieved with the bFN in the DL system than with FN alone. 相似文献
11.
Richter H McCarthy K Nevin KP Johnson JP Rotello VM Lovley DR 《Langmuir : the ACS journal of surfaces and colloids》2008,24(8):4376-4379
The versatility of gold for electrode manufacture suggests that it could be an ideal material for some microbial fuel cell applications. However, previous studies have suggested that microorganisms that readily transfer electrons to graphite do not transfer electrons to gold. Investigations with Geobacter sulfurreducens demonstrated that it could grow on gold anodes producing current nearly as effectively as with graphite anodes. Current production was associated with the development of G. sulfurreducens biofilms up to 40 microm thick. No current was produced if pilA, the gene for the structural protein of the conductive pili of G. sulfurreducens, was deleted. The finding that gold is a suitable anode material for microbial fuel cells offers expanded possibilities for the construction of microbial fuel cells and the electrochemical analysis of microbe-electrode interactions. 相似文献
12.
Sema ASLAN 《Turkish Journal of Chemistry》2021,45(3):661
Electrocatalytic effect of the untreated and TiO2+polyacrylonitrile (PAN) modified discarded battery coal (DBC) and pencil graphite electrodes (PGE) were evaluated in fuel cell (FC) applications. TiO2+PAN solution is coated on PGE and DBC electrodes by electrospinning. According to the FESEM and EDS characterizations, TiO2 and PAN nanofibers are found to be approximately 40 and 240 nm in size. TiO2+PAN/PGE showed the best FC performances with 2.00 A cm–2 current density and 5.05 W cm–2 power density values, whereas TiO2+PAN/DBC showed 0.68 A cm–2 current density and 0.62 W cm–2 power density values. Electrochemical characterizations of PGE and TiO2+PAN/PGE electrodes were investigated by cyclic voltammetry and electrochemical impedance spectroscopy. Finally, long-term FC measurement results of developed electrodes exhibited very reasonable recovery values. Along with the comparison of the electrode performances, the recovery of DBCs as electrodes for renewable energy production has been achieved. 相似文献
13.
Disruption of HepG2 cell adhesion by gold nanoparticle and Paclitaxel disclosed by in situ QCM measurement 总被引:1,自引:0,他引:1
Cell adhesion is a crucial issue for cytotoxicity or anticancer effectiveness for tumor cells. However, how both nanoparticles and drugs affect cell adhesion has not yet been defined. Herein, we report for the first time that gold nanoparticles and Paclitaxel can disrupt adhesion, as well as enhance apoptosis of HepG2 cell individually and synergistically, as observed by in situ measurement using quartz crystal microbalance (QCM). It was also found by MTT assay that gold nanoparticles of low cellular cytotoxicity enhance the antiproliferation and apoptosis of HepG2 cell induced by Paclitaxel. Those findings would be of great potential for biomedical application of nanoparticles. 相似文献
14.
David Sarauli Johannes Tanne Daniel Schäfer Ivo W. Schubart Fred Lisdat 《Electrochemistry communications》2009,11(12):2288-2291
The construction of new mediator-free and fully electroactive protein multilayer electrodes based on DNA and cyt c is demonstrated. The assembly is characterized by a rather high stability, effective electron transfer, and a tunability of the electroactive protein amount by the number of deposited cyt c layers. 相似文献
15.
Cecilia Lete Daniela Berger Cristian Matei Stelian Lupu 《Journal of Solid State Electrochemistry》2016,20(11):3043-3051
The electrodeposition of poly(3,4-ethylenedioxythiophene)-tyrosinase (PEDOT-Ty) biocomposite material onto gold electrode has been achieved by means of a sinusoidal voltages (SV) method. The SV method consists in the superimposition of a sinusoidal voltage (sin wave) with fixed frequency and amplitude onto a d.c. potential. The influence of electrochemical parameters like frequency and amplitude of SV signal, d.c. potential value, deposition time, on the electrodeposition process has been investigated. The biocomposite material has been prepared as a thin layer onto quartz crystals coated with gold. The frequency change of the quartz crystal during the electrodeposition of the biocomposite material was recorded simultaneously with the measured current response. The morphology of the deposited PEDOT-Ty coatings was investigated by scanning electron microscopy. The electrochemical behavior of the biocomposite material in aqueous solution was studied by cyclic voltammetry and electrochemical impedance spectroscopy. The use of d.c. potential value of 0.6 V revealed the contribution of the SV component to the electrodeposition process. The PEDOT-Ty modified electrode was used as an electrochemical biosensor in the voltammetric determination of dopamine in the presence of hydroquinone. The analytical performances of the prepared biosensors were also investigated. 相似文献
16.
Primiceri E Chiriacò MS Dioguardi F Monteduro AG D'Amone E Rinaldi R Giannelli G Maruccio G 《Lab on a chip》2011,11(23):4081-4086
Here an EIS (electrochemical impedance spectroscopy) biochip to detect cell migration is demonstrated. This biochip has been inspired by a traditional transwell assay/modified Boyden chamber and consists of two compartments separated by a porous membrane. This structure (PDMS-based) is aligned to EIS sensors. Cells are seeded in the upper chamber through microfluidic channels. During migration cells go through the pores of the membrane and get in touch with the electrodes that detect migrated cells. The performance of our cell-chip was tested by investigating the migratory ability of hepatocellular carcinoma (HCC) cells as a function of microenvironment. For this purpose we challenged HCC cells to migrate on different extra-cellular matrix (ECM) components including laminin 1, collagen IV and laminin 5. The results reveal that our cell chip provides reliable results that consistently overlap with those obtained with traditional standardized Boyden chambers. Thus, we demonstrate a new, easy tool to study cell migration and to perform automatic assays. This approach is easier and faster than traditional transwell assays and can be suitable for high-throughput studies in drug discovery applications. 相似文献
17.
Guerrette JP Percival SJ Zhang B 《Langmuir : the ACS journal of surfaces and colloids》2011,27(19):12218-12225
We report the fabrication and electrochemical response of a gold nanoband electrode located at the bottom of a glass/epoxy nanotrench, hereafter referred to as a gold nanotrench electrode. Gold nanotrench electrodes of 12.5 and 40 nm in width with various depths from a few tens of nanometers to approximately 4 μm are fabricated and further characterized by cyclic voltammetry. The fabrication of a Au nanotrench electrode follows a simple electrochemical etching process in which a small AC signal is applied to an inlaid Au nanoband electrode submersed in a NaCl solution. The voltammetric behavior of a Au nanotrench electrode is characterized by a quasi-steady-state response at lower scan rates (e.g., <1 V/s for a 12.5-nm-wide electrode). We present an analytical expression for the quasi-steady-state diffusion-limited current of the nanotrench electrode based upon the analysis of the mass-transport resistance. Finite-element simulation of steady-state and transient voltammetric responses of the nanotrench electrodes provides additional insights for the analytical model. Peak-shaped transient voltammetric responses were observed at scan rates as low as 5 V/s for both inlaid and nanotrench electrodes. This result may suggest that the exposed area of the nanoband electrode is much greater than that expected from the fabrication of the inlaid bands. However, the extent to which this is seen is greatly decreased in the nanotrench electrode by a smoothing effect during etching. Our results confirm previous reports of excess overhanging metal and delamination crack contributing significantly to the shape and magnitude of the voltammetric response. 相似文献
18.
Claude Gabrielli Patrick Hemery Pierre Letellier Michele Masure Hubert Perrot Marie-Isabelle Rahmi Mireille Turmine 《Journal of Electroanalytical Chemistry》2004,570(2):275-289
Models of an ion selective electrode involving an ionophore and mobile sites in a membrane are proposed. The first model, called the phase boundary potential model, supposed thermodynamic equilibrium; it allows the concentrations of the various species to be calculated. Then, a kinetic model, which takes into account the ionic transfer at the membrane|solution interfaces, was derived. The impedance of the membrane was calculated. It shows that a membrane with nernstian behavior shows only one capacitive loop in the impedance diagram, which is related to the conductivity and dielectric properties of the material of the membrane. Non-nernstian behavior is related to slow ionic transfer at the membrane|solution interfaces or/and transport limitation of the species in the membrane. Finite rate constants of the ionic transfer lead to a capacitive loop in the middle frequency range, whereas finite rate transport leads to a diffusional impedance in the low frequency range. 相似文献
19.
We considered the role of the superficial atomic structure of polycrystalline gold electrodes on the electrochemical oxidation and the reduction of NO32? photogenerated by trapping of electrons emitted from the electrode. The perturbation of the superficial structure of the electrode by mechanical polishing slows down the oxidation of NO32?. We observed only an activated process in this case. The superficial atomic reorganization of the surface by cyclic polarization makes this reaction faster without perturbation of the short-range diffusion process of reactive species. The production of highly dispersed surfaces (black gold) gives slightly more active electrodes than the preceding one, but influences the short-distance diffusion mode in solution. The last two electrode types exhibit barrier-less and activated processes for the charge-transfer step. 相似文献
20.
A sensitive amperometric sensor for determination of l-histidine was developed using gold electrode modified with Fe(III)-porphyrin bearing three 2,6-di-tert-butylphenol groups and one palmitoyl chain. Two methods of electrode modification were applied: direct chemisorption and embedment into dodecanethiol monolayer. Both types of electrodes were used for detection of l-histidine using Osteryoung square-wave voltammetry. The sensitivity of sensors presented towards l-histidine depends on the method of electrode modification. The detection limits observed for the electrodes incorporating with Fe(III)-porphyrin host by embedment and chemisorption were in 1 and 100 nM ranges, respectively. In addition, the determination of l-histidine with electrode modified by embedment technique was more precise, in comparison to that obtained by the direct chemisorption.Applicability of gold electrodes modified with Fe(III)-porphyrin for the direct electrochemical determination of l-histidine was demonstrated using the artificial matrix mimicking human serum. 相似文献