共查询到20条相似文献,搜索用时 0 毫秒
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设计合成了香豆素衍生物7-(二叔丁基(对硝基苯醚)硅醚)-4-甲基-香豆素(DTBPC),研究了DTBPC在水中对氟离子的响应.在表面活性剂十六烷基溴化铵的存在下,氟离子断裂DTBPC分子中的Si—O键释放出香豆素阴离子,从而"turn-on"体系的荧光,响应时间为3min,检测限为60ppb,表明DTBPC可以在水相中高灵敏、快速、专一性地识别氟离子,有望应用于氟离子荧光传感器. 相似文献
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Construction of a Near‐Infrared Fluorescent Turn‐On Probe for Selenol and Its Bioimaging Application in Living Animals 下载免费PDF全文
Hua Chen Baoli Dong Yonghe Tang Prof. Weiying Lin 《Chemistry (Weinheim an der Bergstrasse, Germany)》2015,21(33):11696-11700
As selenocysteine (Sec) carries out the majority of the functions of the various Se‐containing species in vivo, it is of high importance to develop reliable and rapid assays with biocompatibility to detect Sec. Herein, an NIR fluorescent turn‐on probe for highly selective detection of selenol was designed and synthesized. The probe exhibits large turn‐on signal upon treatment with selenocysteine (R‐SeH), and it was further demonstrated that the new NIR fluorescent probe can be employed to image selenol in living animals. 相似文献
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Lin Yuan Prof. Weiying Lin Yinan Xie Bin Chen Jizeng Song 《Chemistry (Weinheim an der Bergstrasse, Germany)》2012,18(9):2700-2706
Hypochlorous acid (HOCl), a reactive oxygen species (ROS), plays a significant biological role in living systems. However, abnormal levels of HOCl are implicated in many inflammation‐associated diseases. Therefore, the detection of HOCl is of great importance. In this work, we describe the HOCl‐promoted cyclization of rhodamine‐thiosemicarbazides to rhodamine‐oxadiazoles, which is then exploited as a novel design strategy for the development of a new fluorescence turn‐on HOCl probe 2 . On the basis of the fluorescence resonance energy transfer (FRET) signaling mechanism, 2 was further converted into 1 a and 1 b , which represent the first paradigm of FRET‐based ratiometric fluorescent HOCl probes. The outstanding features of 1 a and 1 b include well‐resolved emission peaks, high sensitivity, high selectivity, good functionality at physiological pH, rapid response, low cytotoxicity, and good cell‐membrane permeability. Furthermore, these excellent attributes enable us to demonstrate, for the first time, the ratiometric imaging of endogenously produced HOCl in living cells by using these novel ratiometric probes. We expect that 1 a and 1 b will be useful molecular tools for studies of HOCl biology. In addition, the HOCl‐promoted cyclization reaction of rhodamine‐thiosemicarbazides to rhodamine‐oxadiazoles should be widely applicable for the development of different types of fluorescent HOCl probes. 相似文献
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An Ultrasensitive Cyclization‐Based Fluorescent Probe for Imaging Native HOBr in Live Cells and Zebrafish 下载免费PDF全文
Prof. Kehua Xu Dr. Dongrui Luan Dr. Xiaoting Wang Dr. Bo Hu Dr. Xiaojun Liu Dr. Fanpeng Kong Prof. Bo Tang 《Angewandte Chemie (International ed. in English)》2016,55(41):12751-12754
Bromine has been reported recently as being the 28th essential element for human health. HOBr, which is generated in vivo from bromide, is a required factor in the formation of sulfilimine crosslinks in collagen IV. However, to date, no method for the specific detection of native HOBr in vivo has been reported. Herein, we develop a simple small molecular probe for imaging HOBr based on a specific cyclization catalyzed by HOBr. The probe can be easily synthesized in high yield through a Suzuki cross‐coupling reaction. The probe exhibits ultrahigh sensitivity at the picomole level, in addition to specificity for HOBr and real‐time response. Importantly, without Br? stimulation, this probe reports native HOBr levels in HepG2 cells. Thus, the probe is a promising new tool for imaging endogenous HOBr and may provide a means for finding new physiological functions of HOBr in living organisms. 相似文献
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Alkaline phosphatase (ALP) is associated with many diseases, and its accurate detection is of great significance. Fluorescent compounds with aggregation‐induced emission (AIE) feature show beneficial advantages for serving as fluorescent probes. Herein, an AIE‐active “turn on” probe for ALP detection was synthesized through incorporating a strong electron‐withdrawing group (cyano) in the middle and the recognition moiety phosphate group at the end, thereby rendering a D–A–D structure with a relatively high conjugation degree and good water solubility. It was found that the probe TPE‐CN‐pho is highly sensitive to ALP in aqueous solution. In the presence of ALP, the hydrophilic phosphate group on the probe is rapidly removed, resulting in a decrease in water solubility and subsequent formation of aggregates, thereby achieving aggregation‐induced emission. Moreover, the probe TPE‐CN‐pho has also been successfully applied to imaging ALP in living cells. 相似文献
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A Naphthalimide‐Based Fluorescence “Turn‐On” Probe for the Detection of Pb2+ in Aqueous Solution and Living Cells 下载免费PDF全文
Hio‐Ieng Un Chang‐Bo Huang Dr. Junhai Huang Dr. Chusen Huang Ti Jia Prof. Dr. Lin Xu 《化学:亚洲杂志》2014,9(12):3397-3402
An easily available naphthalimide‐based fluorescent probe NPA for Pb2+ detection was successfully developed. NPA exhibited an obvious fluorescence turn‐on response toward Pb2+ in aqueous solution and in living cells. Moreover, a series of model compounds were rationally designed and synthesized in order to explore the sensing mechanism and binding mode of NPA with Pb2+. 相似文献
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Test‐Strip‐Based Fluorometric Detection of Fluoride in Aqueous Media with a BODIPY‐Linked Hydrogen‐Bonding Receptor 下载免费PDF全文
Dr. Pichandi Ashokkumar Dr. Hardy Weißhoff Dr. Werner Kraus Dr. Knut Rurack 《Angewandte Chemie (International ed. in English)》2014,53(8):2225-2229
The measurement of biologically relevant anions, such as fluoride, is an important task in analytical chemistry, in particular, for dental health and osteoporosis. Although a large number of fluoride probes are known, the applicability under relevant conditions is limited to a few examples. To improve this situation, BODIPY‐amidothiourea dyes with varying hydrogen‐bond donating strengths were developed, the most H‐acidic of which ( 1 c ) could detect F? from an inorganic source (NaF) in 50 % aqueous solution (DMSO/water 1:1, v/v) with 0.01 ppm sensitivity through selective fluorescence quenching by a photoinduced electron‐transfer (PET) process. Use of the probe and a reference dye with a test‐strip assay and a portable and rapidly recording lateral‐flow fluorescence reader made determination of F? in neat aqueous solutions, such as spiked water samples and toothpaste extracts, possible in a self‐referenced manner, achieving a detection limit of 0.2 ppm. 相似文献
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A Pyrene Derivative for Hg2+‐Selective Fluorescent Sensing and Its Application in In Vivo Imaging 下载免费PDF全文
Guang Ke Wang Qi Li Mi Li Yun Zhao Jun Jie Hu Lin E Guo Xiao Ju Zou Bo Liu Xiao Guang Xie Prof. Jun Feng Zhang Prof. Qi Hua Zhao Prof. Ying Zhou 《化学:亚洲杂志》2014,9(3):744-748
An Hg2+‐selective fluorescent sensor ( 1 ) bearing pyrene as a fluorophore was synthesized. A sandwich‐stacking binding mode was formed during the binding process, which increased the excimer fluorescence 22‐fold at 490 nm. Compound 1 was successfully applied in in vivo imaging to trace the enrichment and distribution of mercury in the nervous system, digestive system, and reproductive system of Caenorhabditis elegans, as well as the organs of zebrafish. 相似文献
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The detection and imaging of Zn2+ in biological samples are of paramount interest owing to the role of this cation in physiological functions. This is possible only with molecular probes that specifically bind to Zn2+ and result in changes in emission properties. A “turn‐on” emission or shift in the emission color upon binding to Zn2+ should be ideal for in vivo imaging. In this context, ratiometric and near‐IR probes are of particular interest. Therefore, in the area of chemosensors or molecular probes, the design of fluorophores that allow ratiometric sensing or imaging in the near‐IR region is attracting the attention of chemists. The purpose of this Focus Review is to highlight recent developments in this area and stress the importance of further research for future applications. 相似文献
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Shu‐Li Yao Xue‐Mei Tian Le‐Qian Li Sui‐Jun Liu Teng‐Fei Zheng Yong‐Qiang Chen Da‐Shuai Zhang Jing‐Lin Chen He‐Rui Wen Tong‐Liang Hu 《化学:亚洲杂志》2019,14(20):3648-3654
A new metal‐organic framework (MOF) {[Cd2(bbib)2(ndc)2]?2DMF}n ( JXUST‐1 ) (bbib=1,3‐bis(benzimidazolyl)benzene, H2ndc=1,4‐naphthalenedicarboxylic acid, DMF=N,N‐dimethylformamide) has been solvothermally synthesized and characterized by single‐crystal X‐ray diffraction, PXRD, TGA, IR and elemental analysis. JXUST‐1 exhibits a three‐dimensional 6‐connected pcu topology with a Schläfli symbol {412.63} constructed by [Cd2(CO2)3] secondary building units. Fluorescence studies show that this MOF can sensitively and selectively recognize Al3+ via a fluorescence enhancement effect, and the detection limit is 0.048 ppm. Furthermore, JXUST‐1 displays relatively good thermal and chemical stabilities as well as reusability. All these results suggest JXUST‐1 to be a highly selective and recyclable luminescent sensing material for the detection of Al3+. 相似文献
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A “Distorted‐BODIPY”‐Based Fluorescent Probe for Imaging of Cellular Viscosity in Live Cells 下载免费PDF全文
Hao Zhu Dr. Jiangli Fan Miao Li Jianfang Cao Dr. Jingyun Wang Prof. Dr. Xiaojun Peng 《Chemistry (Weinheim an der Bergstrasse, Germany)》2014,20(16):4691-4696
Cellular viscosity is a critical factor in governing diffusion‐mediated cellular processes and is linked to a number of diseases and pathologies. Fluorescent molecular rotors (FMRs) have recently been developed to determine viscosity in solutions or biological fluid. Herein, we report a “distorted‐BODIPY”‐based probe BV‐1 for cellular viscosity, which is different from the conventional “pure rotors”. In BV‐1 , the internal steric hindrance between the meso‐CHO group and the 1,7‐dimethyl group forced the boron–dipyrrin framework to be distorted, which mainly caused nonradiative deactivation in low‐viscosity environment. BV‐1 gave high sensitivity (x=0.62) together with stringent selectivity to viscosity, thus enabling viscosity mapping in live cells. Significantly, the increase of cytoplasmic viscosity during apoptosis was observed by BV‐1 in real time. 相似文献
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Development of a Small Molecule Probe Capable of Discriminating Cysteine,Homocysteine, and Glutathione with Three Distinct Turn‐On Fluorescent Outputs 下载免费PDF全文
Feiyi Wang Prof. Dr. Zhiqian Guo Xia Li Xiuai Li Prof. Dr. Chunchang Zhao 《Chemistry (Weinheim an der Bergstrasse, Germany)》2014,20(36):11471-11478
The simultaneous discrimination of Cys, Hcy, and GSH by a single probe is still an unmet challenge. The design and synthesis of a small molecule probe MeO‐BODIPY‐Cl (BODIPY=boron dipyrromethene) is presented, which can allow Cys, Hcy, and GSH to be simultaneously discriminated on the basis of three distinct fluorescence turn‐on responses. The probe reacts with these thiols to form sulfenyl‐substituted BODIPY, which is followed by intramolecular displacement to yield amino‐substituted BODIPY. The kinetic rate of the intramolecular displacement reaction determines the observed different sensing behavior. Therefore, the probe responds to Cys, Hcy, and GSH with fluorescence turn‐on colors of yellow, yellow and red, and red, respectively. With this promising feature in hand, the probe was successfully used in imaging of Cys, Hcy and GSH in living cells. 相似文献
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Dr. Binglin Sui Xinglei Liu Dr. Mengyuan Wang Prof. Kevin D. Belfield 《Chemistry (Weinheim an der Bergstrasse, Germany)》2016,22(30):10351-10354
A highly water‐soluble, fluorescence turn‐on sensor for Ca2+ is reported. The sensor affords high selectivity in sensing Ca2+ over other biologically important metal cations. The dissociation constant of the sensor in binding Ca2+ is 0.92 mm . Fluorescence microscopy experiments demonstrate that the sensor is cell‐impermeable and capable of detecting extracellular Ca2+. 相似文献
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A Multisite‐Binding Switchable Fluorescent Probe for Monitoring Mitochondrial ATP Level Fluctuation in Live Cells 下载免费PDF全文
Lu Wang Prof. Dr. Lin Yuan Xian Zeng Dr. Juanjuan Peng Dr. Yong Ni Jun Cheng Er Wang Xu Bikram Keshari Agrawalla Dr. Dongdong Su Dr. Beomsue Kim Prof. Dr. Young‐Tae Chang 《Angewandte Chemie (International ed. in English)》2016,55(5):1773-1776
Adenosine triphosphate (ATP), commonly produced in mitochondria, is required by almost all the living organisms; thus fluorescent probes for monitoring mitochondrial ATP levels fluctuation are essential and highly desired. Herein, we report a multisite‐binding switchable fluorescent probe, ATP‐Red 1 , which selectively and rapidly responds to intracellular concentrations of ATP. Live‐cell imaging indicated that ATP‐Red 1 mainly localized to mitochondria with good biocompatibility and membrane penetration. In particular, with the help of ATP‐Red 1 , we successfully observed not only the decreased mitochondrial ATP levels in the presence of KCN and starvation state, but also the increased mitochondrial ATP levels in the early stage of cell apoptosis. These results indicate that ATP‐Red 1 is a useful tool for investigating ATP‐relevant biological processes. 相似文献