Synthesis,structural, spectroscopic,biological and catalytic activity of Co(II), Ni(II) and Cu(II) complexes of benzilic hydrazide (BH)

Co(II), Ni(II) and Cu(II) chloro complexes of benzilic hydrazide (BH) have been synthesized. Also, reaction of the ligand (BH) with several copper(II) salts, including NO₃ ^?, AcO^?, and SO₄ ^? afforded metal complexes of the general formula [CuLX(H₂O) _n ]·nH₂O, where X is the anion and n = 0, 1 or 2. The newly synthesized complexes were characterized by elemental analysis, mass spectra, molar conductance, UV–vis, IR spectra, magnetic moment, and thermal analysis (TG/DTG). The physico-chemical studies support that the ligand acts as monobasic bidentate towards metal ion through the carbonyl and hydroxyl oxygen atoms. The spectral data revealed that the geometrical structure of the complexes is square planar for Cu (II) complexes and tetrahedral for Co(II) and Ni(II) complexes. Structural parameters of the ligand and its complexes have been calculated. The ligand and its metal complexes are screened for their antimicrobial activity. The catalytic activities of the metal chelates have been studied towards the oxidative decolorization of AB25, IC and AB92 dyes using H₂O₂. The catalytic activity is strongly dependent on the type of the metal ion and the anion of Cu(II) complexes.     

Synthesis,characterization, molecular modeling and antimicrobial activity of 2-(2-(ethylcarbamothioyl)hydrazinyl)-2-oxo-N-phenylacetamide copper complexes

Six Cu(II) complexes of 2-(2-(ethylcarbamothioyl)hydrazinyl)-2-oxo-N-phenylacetamide (H₃APET) have been prepared and characterized by elemental analyses, spectral (IR, UV–vis, ¹H NMR and ESR) as well as magnetic and thermal measurements. The data revealed that the ligand acts as ON bidentate, ONS tridentate or ONNS tetradentate forming structure in which each copper atom is a tetrahedral or tetragonal environment. The bond length, bond angle, HOMO, LUMO, dipole moment and charges on the atoms have been calculated to confirm the geometry of the ligand and the investigated complexes. Kinetic parameters were determined for each thermal degradation stage of the Cu(II) complexes using Coats–Redfern and Horowitz–Metzger methods. Moreover, the ligand and its complexes were screened against bacteria Staphylococcus aureus, Escherichia coli, Candida and fungi, Albicans and Aspergillus flavus using the inhibitory zone diameter.     

Synthesis of several cyclopalladated biacetylmonoxime arylhydrazones

Palladium(II) complexes of biacetylmonoxime arylhydrazones (HL, aryl = phenyl, o-, m-, and p-tolyl, p-chloro- and p-nitrophenyl) and biacetylmonoxime N′-methyl-phenylhydrazone (HL′) have been prepared and characterized. The hydrazomines, HL and HL′, are coordinated through oxime- and hydrazone-nitrogen atoms as a bidentate ligand in the complexes [PdX₂(HL or HL′)] (X = halogen atom) formed under neutral conditions. Under basic conditions the deprotonated complexes [PdX(L or L′)] are obtained. Deprotonation occurs at the oxime group of HL′ and the ligand remains coordinated through oxime- and hydrazone-nitrogen atoms. The other hydrazoximes (HL) are cyclopalladated at the ortho position of the benzene rings and act as an NNC terdentate ligand in the deprotonated complexes [PdXL].     

Synthesis,characterization and biological studies of Co(II), Ni(II), Cu(II) and Zn(II) complexes with pyrral-l-histidinate

The Schiff base ligand, pyrral-l-histidinate(L) and its Co(II), Ni(II), Cu(II) and Zn(II) complexes were synthesized and characterized by elemental analysis, mass, molar conductance, IR, electronic, magnetic measurements, EPR, redox properties, thermal studies, XRD and SEM. Conductance measurements indicate that the above complexes are 1:1 electrolytes. IR data show that the ligand is tridentate and the binding sites are azomethine nitrogen, imidazole nitrogen and carboxylato oxygen atoms. Electronic spectral and magnetic measurements indicate tetrahedral geometry for Co(II) and octahedral geometry for Ni(II) and Cu(II) complexes, respectively. The observed anisotropic g values indicate the presence of Cu(II) in a tetragonally distorted octahedral environment. The redox properties of the ligand and its complexes have been investigated by cyclic voltammetry. Thermal decomposition profiles are consistent with the proposed formulations. The powder XRD and SEM studies show that all the complexes are nanocrystalline. The in vitro biological screening effects of the synthesized compounds were tested against the bacterial species, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus; fungal species, Aspergillus niger, Aspergillus flavus and Candida albicans by the disc diffusion method. The results indicate that complexes exhibit more activity than the ligand. The nuclease activity of the ligand and its complexes were assayed on CT DNA using gel electrophoresis in the presence and absence of H₂O₂.     

Conventional and microwave synthesis,spectral, thermal and antimicrobial studies of some transition metal complexes containing 2-amino-5-methylthiazole moiety

Schiff base metal complexes of Cr(III), Co(II), Ni(II) and Cu(II) derived from 5-chlorosalicylidene-2-amino-5-methylthiazole (HL¹) and 2-hydroxy-1-naphthylidene-2-amino-5-methylthiazole (HL²) have been synthesized by conventional as well as microwave methods. These compounds have been characterized by elemental analysis, FT-IR, FAB-mass, molar conductance, electronic spectra, ¹H-NMR, ESR, magnetic susceptibility, thermal, electrical conductivity and XRD analyses. The complexes exhibit coordination number 4 or 6. The complexes are coloured and stable in air. Analytical data reveal that all the complexes exhibit 1:2 (metal:ligand) ratio. IR data show that the ligand coordinates with the metal ions in a bidentate manner through the phenolic oxygen and azomethine nitrogen. FAB-mass and thermal data show degradation pattern of the complexes. The thermal behaviour of metal complexes shows that the hydrated complexes lose water molecules of hydration in the first step; followed by decomposition of ligand molecules in the subsequent steps. XRD patterns indicate crystalline nature for the complexes. The Schiff bases and metal complexes show good activity against the Gram-positive bacteria; Staphylococcus aureus and Gram-negative bacteria; Escherichia coli and fungi Aspergillus niger and Candida albicans. The antimicrobial results also indicate that the metal complexes are better antimicrobial agents as compared to the Schiff bases.     

Mixed ligand complexes of ruthenium(III), rhodium(III) and iridium(III) with dipicolinic acid and some monobasic bidentate nitrogen,oxygen donor ligands

The trivalent ruthenium, rhodium and iridium complexes of dipicolinic acid and its mixed ligand complexes with several nitrogen, oxygen donor molecules, of types: Na[M(dipic)₂]·2H₂O and [M(dipic)(N-O)]·nH₂O (where M = Ru(III), Rh(III) or Ir(III); dipicH₂ = dipicolinic acid; NOH represents different nitrogen, oxygen donor molecules, viz., picolinic acid, nicotinic acid, isonicotinic acid, glycine, aminophenol, o- or p-aminobenzoic acid), have been synthesized and characterised on the basis of elemental analyses, electrical conductance, magnetic susceptibility measurements and spectral (electronic and infrared) data. The parent dipicolinic acid complexes are found to have a six-coordinate pseudooctahedral structure, whereas for mixed ligand complexes, a polymeric six-coordinate structure has been assigned. Various ligand field and nephelauxetic parameters have also been evaluated.     

Xantphos as cis- and trans-chelating ligand in square-planar platinum(II) complexes. Hydroformylation of styrene with platinum-xantphos-tin(II)chloride system

Platinum(II) complexes of a diphosphine ligand xantphos (4,5-bis(diphenylphosphino)-9,9-dimethyl-xanthene) have been synthesised and characterised by NMR, conductivity and catalytic investigations. In addition to the parent complex cis-PtCl₂(xantphos), trans-platinum(II) complexes of square-planar geometry containing xantphos as a trans-chelating ligand can be obtained due to the large bite angle of the ligand. The platinum-xantphos-tin(II)chloride system acts as active hydroformylation catalyst in the hydroformylation of styrene resulting in high chemo- and regio-selectivities of up to 99.8% and 88%, respectively.     

Palladium(II) complexes of N-[(2-pyridyl)methyliden]-α(or β)-aminonaphthalene: Single crystal X-ray structure of di-chloro-N-[{(2-pyridyl)methyliden}-β-aminonaphthalene]palladium(II), Pd(β-NaiPy)Cl2, spectra and DFT,TD-DFT study

Palladium(II) complexes of N-[(2-pyridyl)methyliden]-α(or β)-aminonaphthalene (α or β-NaiPy) are reported in this work. They are spectroscopically characterized along with some mixed ligand complexes, using diimine and azoimine functions. The single crystal X-ray structure of [Pd(N-(2-pyridyl)methyliden-β-aminonaphthalene)Cl₂] has been determined. Luminescence properties of the complexes exhibit a ligand centered π–π^∗ emission. Quantum yields (?) have been calculated and it has been observed that the complexes of α-NaiPy show higher ? values than the complexes of β-NaiPy. Lifetime measurements suggest bi-exponential decay and the average fluorescence lifetime varies from 1.4 to 6.8 ns. The spectroscopic properties are correlated with DFT and TD-DFT calculations in two complexes, Pd(β-NaiPy)X₂ (X = Cl, I) and they are compared with the free ligand results.     

Potentiometric and conductometric studies of malonyl bis(salicyloylhydrazone) and divalent metal complexes

A series of complexes of divalent transition metal ions with malonyl bis(salicyloylhydrazone) (H₄MSH) have been prepared and characterized with the help of conductometric, potentiometric methods. The proton–ligand and metal–ligand stability constants were obtained pH-metrically. The electrical conductivity of solid complexes was measured at 289 K. The low molar conductance values observed for these complexes indicate that, they are non-electrolytes. They are soluble to a limited extent in DMF and DMSO. The elemental analyses of the complexes indicate that the complexes have 1:1 and 2:1 (M:L) stoichiometry with the existence of water, chloride, acetone molecules inside the coordination sphere as evidence from the IR spectral studies. Further, the complexes have been formulated by comparing C, H, N & metal analysis data, and UV–visible spectra of the complexes have been discussed. The protonation constants of the ligand and the stability constants of their metal complexes will be evaluated potentiometrically. The stoichiometric ratios of the complexes formed in solution will be evaluated applying the molar ratio (spectrophotometric) method and confirmed conductometrically.     

Synthesis,spectroscopic, DNA cleavage and antibacterial activity of binuclear schiff base complexes

The binuclear Schiff base complexes are formed newly using different transition metals at their stable oxidation state as Cu(II), Ni(II), and VO(II). 3,3′,4,4′-tetraminobiphenyl and 2-aminobenzaldehyde were condensed to form a new Schiff base ligand having an two N₄ group responsible for better chelating to the metal centers. The ligand and their complexes have been established by analytical, spectral and electrochemical data. The interaction studies of the complexes with CT-DNA were carried out using cyclic voltammetry, viscosity measurements and fluorescence spectroscopy. The free ligand and their metal complexes were screened for their antimicrobial activities against the following species: Klebsiella pneumoniae, Escherichia coli and Staphylococcus aureus. A comparative study of minimum inhibitory concentration (MIC) values of the Schiff base and its complexes indicate that the metal complexes exhibit higher antibacterial activity than the free ligand.     

Synthesis and crystal structures of copper(II) and silver(I) complexes of a biphenyl-bridged bipyrazolyl ligand

A semirigid bipyrazolyl ligand, 4,4??-bis[(3??,5??-diethyl-1H-pyrazol-4??-yl)methylene)]-1,1??-biphenyl (H₂L), and four of its Ag(I) and Cu(II) complexes have been prepared and structurally characterized. X-ray analysis demonstrates that the Ag(I) complexes are dinuclear molecular rectangle, while the Cu(II) complexes display a twisted rectangular structure. Two different conformations, namely cis and trans, have been observed for this bipyrazolyl ligand.     

Synthesis,spectral, thermal,potentiometric and antimicrobial studies of transition metal complexes of tridentate ligand

A series of metal complexes of Cu(II), Ni(II), Co(II), Fe(III) and Mn(II) have been synthesized with newly synthesized biologically active tridentate ligand. The ligand was synthesized by condensation of dehydroacetic acid (3-acetyl-6-methyl-(2H) pyran-2,4(3H)-dione or DHA), o-phenylene diamine and fluoro benzaldehyde and characterized by elemental analysis, molar conductivity, magnetic susceptibility, thermal analysis, X-ray diffraction, IR, ¹H-NMR, UV–Vis spectroscopy and mass spectra. From the analytical data, the stoichiometry of the complexes was found to be 1:2 (metal:ligand) with octahedral geometry. The molar conductance values suggest the non-electrolyte nature of metal complexes. The IR spectral data suggest that the ligand behaves as a dibasic tridentate ligand with ONN donor atoms sequence towards central metal ion. Thermal behaviour (TG/DTA) and kinetic parameters calculated by the Coats–Redfern and Horowitz–Metzger method suggest more ordered activated state in complex formation. To investigate the relationship between stability constants of metal complexes and antimicrobial activity, the dissociation constants of Schiff bases and stability constants of their binary metal complexes have been determined potentiometrically in THF–water (60:40%) solution at 25 ± 1 °C and at 0.1 M NaClO₄ ionic strength. The potentiometric study suggests 1:1 and 1:2 complexation. Antibacterial and antifungal activities in vitro were performed against Staphylococcus aureus, Escherichia coli and Aspergillus niger, Trichoderma, respectively. The stability constants of the metal complexes were calculated by the Irving–Rosotti method. A relation between the stability constant and antimicrobial activity of complexes has been discussed. It is observed that the activity enhances upon complexation and the order of antifungal activity is in accordance with stability order of metal ions.     

Synthesis, spectroscopic, and thermal studies of some Hg(II) and Cd(II) coordination compounds of N,N-bis[(E)-3-(phenylprop)-2-enylidene]propanediamine

Some new coordination compounds of cadmium(II) and mercury(II) with N,N-bis[(E)-3-(phenylprop)-2-enylidene]propanediamine (L) as a new bidentate Schiff base ligand with general formula MLX₂ (X = Cl^?, Br^?, I^?, SCN^?, and N₃ ^?) have been prepared. They were characterized by elemental analysis, FT-infrared (FT-IR) and Ultraviolet–Visible spectra, ¹H- and ¹³C-NMR spectra. The reasonable shifts of FT-IR and NMR spectral signals of the complexes with respect to the free ligand confirm well coordination of ligand and anions(X-) in inner sphere coordination mode. The thermal behavior of the complexes from room temperature to 800 °C shows weight loss by decomposition of the anions and ligand segments in the subsequent steps. The results showed that cadmium complexes have no water molecules (neither as lattice nor as coordinated water) and are decomposed in two temperature steps except about cadmium thiocyanate complex that is decomposed in three steps. Final residual contents of cadmium complexes are suggested to be cadmium oxide or sulfide. Mercury complexes were decomposed in three to four temperature steps. Mercury bromide and azide complexes leave out a little amount of mercury oxide in final, while mercury chloride, iodide, and thiocyanate complexes were found to be completely decomposed without any residual matter.     

Metal complexes of pyrimidine derived ligands – Syntheses,characterization and X-ray crystal structures of Ni(II), Co(III) and Fe(III) complexes of Schiff base ligands derived from S-methyl/S-benzyl dithiocarbazate and 2-S-methylmercapto-6-methylpyrimidine-4-carbaldehyde

Two new pyrimidine based NNS tridentate Schiff base ligands S-methyl-3-((2-S-methyl-6-methyl-4-pyrimidyl)methyl)dithiocarbazate [HL₁] and S-benzyl-3-((2-S-methyl-6-methyl-4-pyrimidyl)methyl)dithiocarbazate [HL₂] have been synthesised by the 1:1 condensation of 2-S-methylmercapto-6-methylpyrimidine-4-carbaldehyde and S-methyl/S-benzyl dithiocarbazate. A Ni(II) complex of HL₁ and Co(III) and Fe(III) complexes of HL₂ have been prepared and characterized by elemental analyses, molar conductivities, magnetic susceptibilities and spectroscopic studies. All the bis-chelate complexes have a distorted octahedral arrangement with an N₄S₂ chromophore around the central metal ion. Each ligand molecule binds the metal ion using the pyrimidyl and azomethine nitrogen and thiolato sulfur atoms (except in the nickel complex, one ligand molecule uses the thione sulfur in lieu of thiolato sulfur atom). In the Ni(II) complex, one of the ligand molecules behaves as a neutral tridentate and the other molecule functions as a uninegative tridentate, whereas in the Co(III) and Fe(III) complexes, the ligand molecules behave as monoanionic tridentate. All the complexes were analyzed by single crystal X-ray diffraction and significant differences concerning the distortion from an octahedral geometry of the coordination environment were observed.     

Synthesis,characterization, antimicrobial and diuretic study of Mg(II), Mn(II), Fe(II) and VO(II) complexes of chemotherapeutic importance

The synthesis, characterization and diuretic activity of four new biologically active complexes of Mg(II) and VO(II) with bidentate Schiff base ligand acetazolamide–salicylaldimine (L) obtained from the inserted condensation of 5-acetamido-1,3,4-thiadiazole-2-sulphonamide (acetazolamide) with salicylaldehyde in a 1:1 molar ratio have been reported. Using this bidentate ligand complexes of Mg(II), Mn(II), Fe(II) and VO(II) with general formula ML₂ have been synthesized. The synthesized complexes were characterized by several techniques using elemental analysis, FT-IR, electronic spectra, TGA, mass, particle size analysis and molar conductance measurements. The elemental analysis data suggest the stoichiometry to be 1:2 [M:L]. The molar conductance measurements suggest non-electrolytic nature of the complexes. Infrared spectral data agreed with the coordination to the central metal ion through deprotonated phenolic oxygen and azomethine nitrogen atoms. On the basis of spectral studies, octahedral geometry is suggested for Mg(II), Mn(II), Fe(II) and square pyramidal geometry is suggested for VO(II) complexes. The pure drug, synthesized ligand and metal(II) complexes were screened for their antimicrobial activities against Eschericia coli, Bacillus subtilis, Aspergillus niger and Aspergillus flavous. The results show that the metal complexes were more active than the ligand and pure drug against these microbial species as expected. The ligand and its Mg(II) complexes was screened for their diuretic activity also.     

Transition metal chelates: Synthesis,physicochemical, thermal,and biological studies

The transition metal complex of Mn(II), Co(II), Ni(II), Cu(II), Ti(III), Cr(III), Fe(III), Zr(IV), and UO₂(VI) ion with a Schiff’s base ligand derived from 2-hydroxy-[2-oxo-1,2-dihydro-3H-indol-3-ylidene]-benzohydrazide have been prepared. The complexes have been characterized by elemental analysis data, IR and electronic absorption spectra, magnetic moments, and thermogravimetric analysis data. The complexes of the 1: 1 metal-to-ligand stoichiometry have been formed. The physico-chemical data have suggested the octahedral geometry for all the complexes except for Cu(II); the Cu(II) complex has been square planar. Thermal analysis data of the ligand and its complexes have been analyzed, and the kinetic parameters have been determined using the Horowitz–Metzger method. According to the solid-state electrical conductivity measurements, the ligand and its complexes are semiconducting in nature. The antimicrobial activity of the ligand and the complexes towards E. coli, S. typhi, P. aeruginosa, and S. aureus has been tested by the disc diffusion method.     

Titanocene complexes of the N-methyl and N-phenyl-1,3-thiazoline-2-thione-4,5-dithiolates and 4,5-diselenolate ligands

Cp₂Ti(dithiolene) and Cp₂Ti(diselenolene) complexes containing the N-methyl-1,3-thiazoline-2-thione-4,5-dithiolate ligand (Me-thiazdt), the N-phenyl-1,3-thiazoline-2-thione-4,5-dithiolate ligand (Ph-thiazdt) and the N-methyl-1,3-thiazoline-2-thione-4,5-diselenolate ligand (Me-thiazds) have been synthesized. Three approaches have been developed in order to generate the dithiolene or the diselenolene ligands which were reacted with Cp₂TiCl₂ to form the corresponding heteroleptic complexes. Their X-ray crystal structures, UV-Vis absorption spectra as well as their redox properties, determined by cyclic voltammetry have been investigated and discussed. Variable-temperature ¹H NMR experiments have been performed in order to determine the activation energies of the chelate ring inversion.     

Synthesis, electrochemical and structural characterization of novel azacrown ether containing macrocyclic redox-active vic-dioxime ligand and its mononuclear transition metal complexes: Application of DEPT, HSQC, HMBC-NMR and cyclic voltammetry

This paper presents a new azacrown containing vic-dioxime; anti-N-(4-aminophenyl)aza-15-crown-5-glyoxime (LH₂), and its mononuclear nickel(II), copper(II), cobalt(II), cadmium(II) and zinc(II) complexes. The azacrown moieties appended at the periphery of the oxime provide solubility for the vic-dioxime ligand and complexes in common organic solvents. The mononuclear M(LH)₂ (M = Ni and Cu), M(LH)₂(H₂O)₂ (M = Co) and [M(LH)(H₂O)(Cl)] (M = Cd and Zn) complexes have been obtained with the metal:ligand ratios of 1:2 and 1:1. The structure of the ligand is confirmed by elemental analysis, Fourier transform infrared (FT-IR), ultraviolet-visible (UV-Vis), mass spectrometry (MS), one-dimensional (1D) ¹H, ¹³C NMR, distortionless enhancement by polarization transfer (DEPT) and two-dimensional (2D) heteronuclear single quantum coherence (HSQC) and heteronuclear multiple bond correlation (HMBC) techniques. The structures of the complexes are confirmed by elemental analyses, MS, UV-Vis, FT-IR and ¹H, ¹³C NMR techniques. Redox behaviors of the ligand and its complexes have been investigated by cyclic voltammetry at the glassy carbon electrode in 0.1 M TBATFB in DMSO. The antibacterial activity was studied against Staphylococcus aureus ATCC 29213, Streptococcus mutans RSHM 676, Enterococcus faecalis ATCC 29212, Lactobacillus acidophilus RSHM 06029, Escherichia coli ATCC 25922, Pseudomonasaeruginosa ATCC 27853. The antimicrobial test results indicate that all the complexes have low levels of antibacterial activity against both Gram negative and Gram positive bacterial species.     

Synthesis,spectroscopic characterization and thermal studies of some rare earth metal complexes of unsymmetrical tetradentate Schiff base ligand

The solid complexes of La(III), Ce(III), Pr(III), Nd(III), Sm(III) and Gd(III) with 4-hydroxy-3-(1-{2-(2-hydroxy-benzylidene)-aminophenylimino}-thyl)-6-methy-pyran-2-one (H₂L) derived from o-phenylenediamine, 3-acetyl-6-methyl-(2H)pyran,2,4(3H)-dione (dehydroacetic acid or DHA) and salicylic aldehyde have been synthesized and characterized by elemental analysis, conductometry, magnetic susceptibility, UV–visible, FTIR, ¹H NMR spectra, X-ray diffraction, and thermal analysis and screened for antimicrobial activity. The FTIR spectral data suggest that the ligand behaves as a dibasic tetradentate ligand with ONNO donar atoms sequence towards central metal ion. From the microanalytical data, the stoichiometry of the complexes has been found to be 1:1 (metal:ligand). The physico-chemical data suggest distorted octahedral geometry for La(III), Ce(III), Pr(III), Nd(III), Sm(III) and Gd(III) complexes. The X-ray diffraction data suggest monoclinic crystal system for La(III) and Ce(III) and orthorhombic crystal system for Pr(III) and Nd(III) complexes. Thermal behaviour (TGA/DTA) of the complexes was studied and kinetic parameters were determined by Horowitz–Metzger and Coats–Redfern methods. The ligand and their metal complexes were screened for antibacterial activity against Staphylococcus aureus, Escherichia coli and Bacillus sp. Fungicidal activity against Aspergillus niger, Trichoderma and Fusarium oxysporum.     

Synthesis,spectral characterization,theoretical, antimicrobial,DNA interaction and in vitro anticancer studies of Cu(II) and Zn(II) complexes with pyrimidine-morpholine based Schiff base ligand

Novel Cu(II) (1) and Zn(II) (2) complexes with 4-(1-(4-morpholinophenyl)ethylideneamino)pyrimidine-5-carbonitrile) (L) have been synthesized and characterized by various spectroscopic and analytical techniques. DFT (density functional theory) studies result confirms that, LMCT mechanism have been done between L and M(II) ions. The antimicrobial studies indicate that the ligand L and complexes 1 & 2 exhibit higher activity against the E. coli bacteria and C. albicans fungi. The groove binding mode of ligand L and complexes 1 & 2 with CT-DNA have been confirmed by electronic absorption, competitive binding, viscometric and cyclic voltammetric studies. The electronic absorption titration of ligand L and complexes 1 & 2 with DNA have been carried out in different buffer solutions (pH 4.0, 7.0 & 10.0). The K_b values of ligand L and complexes 1 & 2 are higher in acidic buffer at pH 4.0 (K_b = 2.42 × 10⁵, L; 2.8 × 10⁵, 1; 2.65 × 10⁵, 2) and the results revealed that, the interaction of synthesized compounds with DNA were higher in the acidic medium than basic and neutral medium. Furthermore, CT-DNA cleavage studies of ligand L and complexes 1 & 2 have been studied. The in vitro anticancer activities results show that complexes 1 & 2 have moderate cytotoxicity against cancer cell lines and low toxicity on normal cell line than ligand L.