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1.
A novel molecularly imprinted polymer solid-phase extraction (MISPE) with flow-injection chemiluminescence (CL) was developed
for the determination of pazufloxacin mesilate (PZFX). The molecularly imprinted polymer (MIP) was synthesized by using PZFX
as the imprinting molecule. A glass tube packed the particles of the MIP was employed as MISPE micro-column, which was connected
into the sampling loop of the eight-way injection valve for on-line selective preconcentration and extraction of PZFX. The
eluent of acetonitrile:acetic acid (9:1, v:v) was used as carrier for eluting the adsorbed PZFX to react with the mixture
of cerium(IV) and sodium sulfite in the flow cell to produce strong CL. The relative intensity of CL was linear to PZFX concentration
in the range from 2.5 × 10−9 to 2.5 × 10−7 g mL−1. The limit of detection was 7 × 10−10 g mL−1 (3 σ) and the relative standard deviation for 5 × 10−8 g mL−1of PZFX solution was 3.7% (n = 7). This method has been applied to the determination of PZFX in human urine. 相似文献
2.
M. A. Campanero A. M. Zamarreño M. Simón M. C. Dios J. R. Azanza 《Chromatographia》1997,46(7-8):374-380
Summary A simple and rapid liquid chromatographic method has been developed for the determination of therapeutic levels of piperacillin
(I) and ceftazidime (II) in human plasma. Plasma and p-propionamidophenol (internal standard) were precipitated with methanol
(I) or 20% trichloroacetic acid (II). The supernatant was analysed on a 5 μm Spherisorb ODS C18 column with acetonitrile-0.05 M phosphate buffer pH 3.8 as mobile phase and ultraviolet detection at 254 nm. The calibration
graph was linear from 10 to 250 μg mL−1, for (I), and from 5 to 200 μg mL−1 for (II). Intra and inter-day CV did no exceed 2.29% for (I), and were 10.76–11.13%–2.00–5.62 for (II) at concentrations
of 10 μg mL−1 and 250 μg mL−1. 相似文献
3.
Yan Liang Jie Sun Lin Xie An Kang Yuan Xie Wei-Dong Chen Hua Lv Guang-Ji Wang 《Chromatographia》2007,66(3-4):165-170
A sensitive and specific liquid chromatographic method with electrospray ionization mass spectrometry (LC–ESI-MS) has been
developed and validated for identification and quantification of mitiglinide in human urine. A simple liquid–liquid extraction
procedure was followed by separation on a C18 column with gradient elution, and detection using a single-quadrupole mass spectrometer in selected-ion-monitoring (SIM)
mode. The method was tested using six different batches of urine. Linearity was established for the mitiglinide concentrations
in the range 0.005–1.0 μg mL−1, with a coefficient of determination (r) of 0.9998 and good back-calculated accuracy and precision. Intra- and inter-day precision (as RSD, %) was below 10% and
accuracy for mitiglinide ranged from 85 to 115%. The lower limit of quantification was reproducible at 0.002 μg mL−1 for 500 μL urine. The proposed method enables unambiguous identification and quantification of mitiglinide in pre-clinical
and clinical studies. 相似文献
4.
Summary A simple, rapid and accurate, routine-HPLC method is described for simultaneous determination of acetaminophen, caffeine and
chlorpheniramine maleate in a new tablet formulation Chromatographic separation of the three pharmaceuticals was achieved
on a Hypersil CN column (150×5.0 mm, 5 μm) using a mobile phase comprising a mixture of acetonitrile, an ion-pair solution
and tetrahydrofuran (13:14:87, v/v,pH4.5). The flow-rate was changed from 1.0 mL min−1 (in 0≈7.5 min) to 1.8 mL min−1 (after 3.5 min). was complete in <10 min. The method was validated for system suitability, linearity, accuracy, precision,
limits of detection and quantitation, and robustness. Linearity, accuracy and precision were found to be acceptable over the
ranges 31.6≈315.8 μg mL−1 for acetaminophen, 9.5≈94.6 μg mL−1 for caffeine and 1.4≈13.8 μg mL−1 for chlorpheniramine maleate. 相似文献
5.
Qing Li Liang Xu Ting-Ting Wang Ying Jia Zhi-Wei Wang Kai-Shun Bi 《Chromatographia》2008,67(7-8):627-631
Aidi injection is a clinical medicine used in China for the treatment of cancer. Calycosin-7-O-β-d-glucoside is the main effective components of the formulas. In this study, a high performance liquid chromatographic (LC)
method was developed to quantify calycosin-7-O-β-d-glucoside in rat plasma using a liquid–liquid extraction and ultraviolet (UV) absorbance detection. LC analysis was performed
on a Diamonsil C18 column (200 × 4.6 mm i.d., 5 μm particle size) with isocratic mobile phase consisting of acetonitrile–0.05% phosphoric acid
(19.5:80.5, v/v) of a flow rate of 1.0 mL min−1. The linear range was 0.11–17.6 μg mL−1 and the low quantification limit was 0.11 μg mL−1 (S/N = 10). The intra- and inter-day relative standard deviations (RSD) in the measurement of quality control (QC) samples
0.11, 0.22, 1.32 and 8.80 μg mL−1 ranged from 4.1 to 6.3 and 4.3 to 6.2%, respectively. The accuracy was from −6.7 to 4.3% in terms of relative error (RE).
Calycosin-7-O-β-d-glucoside was stable in storage at −20 °C for 2 weeks and stable after three freeze–thaw cycles in rat plasma. This method
was validated for specificity, accuracy, precision and was successfully applied to pharmacokinetic study of calycosin-7-O-β-d-glucoside in rat plasma after intravenous administration of Aidi lyophilizer. 相似文献
6.
Determination of Aldehydes and Ketones in Fuel Ethanol by High-Performance Liquid Chromatography with Electrochemical Detection 总被引:1,自引:0,他引:1
A. A. Saczk L. L. Okumura M. F. de Oliveira M. V. B. Zanoni N. R. Stradiotto 《Chromatographia》2006,63(1-2):45-51
A new methodology was developed for analysis of aldehydes and ketones in fuel ethanol by high-performance liquid chromatography
(HPLC) coupled to electrochemical detection. The electrochemical oxidation of 5-hydroxymethylfurfural, 2-furfuraldehyde, butyraldehyde,
acetone and methyl ethyl ketone derivatized with 2,4-dinitrophenylhydrazine (DNPH) at glassy carbon electrode present a well
defined wave at +0.94 V; +0.99 V; +1.29 V; +1.15 V and +1.18 V, respectively which are the basis for its determination on
electrochemical detector. The carbonyl compounds derivatized were separated by a reverse-phase column under isocratic conditions
with a mobile phase containing a binary mixture of methanol / LiClO4(aq) at a concentration of 1.0 × 10−3 mol L−1 (80:20 v/v) and a flow-rate of 1.1mL min−1 . The optimum potential for the electrochemical detection of aldehydes-DNPH and ketones-DNPH was +1.0 V vs. Ag/AgCl. The
analytical curve of aldehydes-DNPH and ketones-DNPH presented linearity over the range 5.0 to 400.0 ng mL−1, with detection limits of 1.7 to 2.0 ng mL−1 and quantification limits from 5.0 to 6.2 ng mL−1, using injection volume of 20 μL. The proposed methodology was simple, low time-consuming (15 min/analysis) and presented
analytical recovery higher than 95%. 相似文献
7.
Summary A rapid and accurate HPLC method is described for the simultaneous determination of acetaminophen, dextromethorphen hydrobromide
and pseudoephedrine hydrochloride in a new cold formulation. Chromatographic separation of the three pharmaceuticals was performed
on a Hypersil CN column (150×5.0 mm, 5 μm) with a mobile phase mixture of an ion-pairing solution, methanol and acetonitrile
(25:57:18, v/v), at a flow rate of 1.0 mL min−1, with detection at 220 nm. Separation was complete in less than 10 min. The method was validated for linearity, accuracy,
precision, limit of quantitation and robustness. Linearity, accuracy, and precision were found to be acceptable over the ranges
of 2.06∼20.6 μg·mL−1 for acetaminophen, 0.202∼2.02 mg·mL−1 for pseudoephedrine hydrochloride and 0.042∼1.06 mg·mL−1 for dextromethorphen hydrobromide. 相似文献
8.
K. Watanabe H. Hattori M. Nishikawa A. Ishii T. Kumazawa H. Seno O. Suzuki 《Chromatographia》1997,44(1-2):55-58
Summary Cocaethylene together with cocaine spiked in human whole blood has been found measurable at high sensitivities by capillary
gas chromatography with surface ionization detection. The drugs could be rapidly extracted by Sep-Pak C18 cartridges with recovery of more than 60%. The calibration curves for both cocaethylene and cocaine using cocapropylene as
internal standard were linear in the range 50–300 pmol mL−1 of whole blood. The detection limits of cocaethylene and cocaine were 5–10 pmol mL−1 (0.1–0.2 pmol on column if recovery is 100%). Cocaethylene could be determined for whole blood obtained from rats (ca. 200
g body wt.), which had received subcutaneous injection of 10 mg cocaine hydrochloride and 2.0 mL of 30% (v/v) ethanol 3 h
before sampling; the mean levels of cocaethylene and cocaine were 101 and 1230 pmol mL−1, respectively. 相似文献
9.
Schettgen T Tings A Brodowsky C Müller-Lux A Musiol A Kraus T 《Analytical and bioanalytical chemistry》2007,387(8):2783-2791
Analysis of biomarkers in exhaled breath condensate (EBC) is a non-invasive method for investigating the effects of different
diseases or exposures, on the lungs and airways. N
ɛ-carboxymethyllysine (CML) is an important biomarker of advanced glycation end products (AGEs). A method has been developed
for simultaneous determination of CML and its precursor, the amino acid lysine, in exhaled breath condensate (EBC). After
addition of labelled internal standards (d-4-CML; d-4-lysine), the EBC was concentrated by freeze-drying. Separation and detection
of the analytes were performed by hydrophilic-ion liquid chromatography coupled with tandem mass-spectrometric detection (HILIC–MS–MS).
The limits of quantification were 10 pg mL−1 EBC and 0.5 ng mL−1 EBC for CML and lysine, respectively. The relative standard deviation of the within-series precision was between 2.8 and
7.8% at spiked concentrations between 40 and 200 pg mL−1 for CML and between 6 and 20 ng mL−1 for lysine. Accuracy for the analytes ranged between 89.5 and 133%. The method was used for the analysis of EBC samples from
ten healthy persons from the general population and ten persons receiving dialysis. CML and lysine were detected in all EBC
samples with median values of 19 pg mL−1 CML and 11.9 ng mL−1 lysine in EBC of healthy persons and 25 pg mL−1 CML and 9.5 ng mL−1 lysine in EBC of dialysis patients. 相似文献
10.
Neng Zhou Yi-Zeng Liang Ben-Mei Chen Ping Wang Xian Chen Feng-Ping Liu 《Chromatographia》2007,66(7-8):481-486
A rapid, simple and specific liquid chromatography-electrospray ionization mass spectrometry method has been developed and
validated for the determination of hydroxyzine hydrochloride in human plasma. Samples were separated using a Thermo Hypersil-HyPURITYC18
reversed-phase column (150 mm × 2.1 mm i.d., 5 μm). The mobile phase consisted of 50 mM ammonium acetate (pH 4.0)–methanol–acetonitrile
(45:36:19, v/v). Hydroxyzine and its internal standard were measured by electrospray ion source in positive selective ion monitoring mode.
The method was validated with a linear range of 1.56–200.0 ng mL−1 and the lowest limit of quantification was 1.56 ng mL−1 for hydroxyzine hydrochloride (r
2= 0.9991). The extraction efficiencies were about 70% and recoveries of the method were in the range of 93.5–104.4%. The intra-day
relative standard deviation (RSD) was less than 8.0% and inter-day RSD was within 7.4%. QC samples were stable when kept at
ambient temperature for 12 h at −20 °C for 30 days and after four freeze–thaw cycles. The method has been successfully applied
to the evaluation of pharmacokinetics and bioequivalence of two hydroxyzine hydrochloride formulations in 12 healthy Chinese
volunteers after an oral dose of 25 mg. 相似文献
11.
M. A. Campanero B. Calahorra E. García-Quetglás M. Escolar J. Honorato 《Chromatographia》1998,48(7-8):555-560
Summary A sensitive liquid chromatographic assay for the quantitative determination of the opioid analgesic tramadol and its active
metabolite is described. Fluconazole was used as internal standard. The assay involved a singletert-butyl methyl ether extraction and LC analysis with fluorescence detection. Chromatography was at 30°C pumping an isocratic
mobile phase of acetonitrile-water (19∶81, v/v) containing 0.06M NaH2PO4 and 0.05M triethylamine, adjusted to pH 7.90, at 1 mL min−1 through a reversed-phase, 250×4 mm base-stable column. The limit of quantitation of tramadol and its active metabolite was
1 ng mL−1, only 0.5 mL plasma sample was required for the determination. The calibration curve was linear from 1–1000 ng mL−1. Intra and inter-day precision (C.V.) did not exceed 10%. Mean recoveries of 96.38% for tramadol and 96.62% forO-demethyltramadol with CVs of 0.43% and 1.46% were obtained. Applicability of the method was demonstrated by a pharmacokinetic
study on normal volunteers who received 100 mg tramadol intravenously. 相似文献
12.
Summary A rapid, sensitive and selective high-performance liquid chromatographic method has been developed for the determination of
sphingosine in human serum. After precipitation with methanol, the samples were extracted using Carbopack B disposable columns;
the sphingosine was eluted with 0.05 M hydrochloric acid in methanol-dichloromethane (20∶80, v/v) and the extract evaporated
to dryness at 40°C. The sample residue was then reconstituted with methanol and reacted with o-phthaldialdehyde reagent to
produce a fluorescent compound. Separation was performed using an LC-18 column with 0.05 M phosphate buffer (pH 7)-methanol-acetonitrile
(15∶80∶5, v/v) as mobile phase. Fluorescence detection was performed with excitation and emission wavelengths of 340 and 455
nm, respectively. The serum extract was re-analyzed with a cyano LC column to minimize the possibility of false positive results.
The possible interference of compounds having a structure similar to that of sphingosine was evaluated. The mean recovery
of sphingosine was >94.5%. The limit of detection of the assay was 1 ng mL−1. The between-run and within-run coefficients of variation for replicate analyses were <4.0% and <3.4%, respectively. The
levels of free sphingosine in the serum of 40 normal subjects (20 male and 20 female) was investigated; the average level
was 81.6±41.1 ng mL−1 (mean ±S.D.) for males and 85.5±33.7 ng mL−1 for females. 相似文献
13.
Artem U. Kulikov 《Chromatographia》2007,66(5-6):303-309
A simple micellar liquid chromatographic technique for deltamethrin determination was developed and validated. The method
provided to be suitable for deltamethrin determination in pediculicide shampoo. Kromasil C18 column (150 mm×4.6 mm, 5 μm)
and mobile phase −0.12 M sodium dodecyl sulfate with 9% (v/v) 1-butanol were used for deltamethrin separation. Detection wavelength was 265 nm. The retention time was about 15 min. Different
validation parameters were evaluated. The specificity of the method was demonstrated. Linearity was established in the range
10–40 μg L−1. The limits of detection and quantitation were 1.06 and 3.22 μg mL−1, respectively. The method showed excellent accuracy (100.6%) and precision (repeatability) gave a relative standard deviation
of less than 1%. The influence of the various method parameters (robustness study) was also studied. 相似文献
14.
C. Flores-Pérez H. Juárez-Olguín J. Flores-Pérez B. Ramírez-Mendiola J. Bobadilla Chávez 《Chromatographia》2005,62(7-8):373-377
The aim of the present study was to develop a simple method to measure plasma levels of propafenone by liquid chromatography
with a C18 reverse-phase column and fluorescence detection, without previous derivatization of the sample. Linearity was assessed
in the range from 50 to 1000 ng mL−1 and had a correlation coefficient of 0.999. The inter- and intra-day coefficients of variation were below 5%. The limits
of detection and quantification were 15 ng mL−1 and 50 ng mL−1, respectively. Drug levels were determined satisfactorily in two patients. A simple and reliable method was developed, especially
useful in children with heart failure under treatment with propafenone. 相似文献
15.
A simple reversed-phase high-performance liquid chromatographic (HPLC) method has been developed for the simultaneous determination
of the antiepileptic drugs (AEDs) zonisamide (ZNS), primidone (PRI), lamotrigine (LTG), phenobarbital (PB), phenytoin (PHT),
oxcarbazepine (OXC), and carbamazepine (CBZ) and two of their active metabolites, monohydroxycarbamazepine (MHD) and carbamazepine
10,11-epoxide (CBZE) in human plasma. Plasma (100 μL) was pretreated by deproteinization with 300 μL methanol containing 20 μg mL−1 propranolol hydrochloride as internal standard. HPLC was performed on a C8 column (4.6 mm × 250 mm; particle size 5 μm) with methanol–acetonitrile–0.1% trifluoroacetic acid, 235:120:645 (v/v), as mobile phase at a flow rate of 1.5 mL min−1. ZNS, OXC, and CBZ were monitored by UV detection at 235 nm, and PRI, LTG, MHD, PB, PHT, and CBZE by UV detection at 215 nm.
Relationships between response and concentration were linear over the concentration ranges 1–80 μg mL−1 for ZNS, 5–50 μg mL−1 for PRI, 1–25 μg mL−1 for LTG, 1–50 μg mL−1 for MHD, 5–100 μg mL−1 for PB, 1–10 μg mL−1 for CBZE, 0.5–25 μg mL−1 for OXC, 1–50 μg mL−1 for PHT, and 1–25 μg mL−1 for CBZ. Intra-day and inter-day reproducibility were adequate (coefficients of variation were ≤11.6%) and absolute recovery
ranged from 95.2 ± 6.13 to 107.7 ± 7.76% for all the analytes; for the IS recovery was 98.69 ± 1.12%. The method was proved
to be accurate, reproducible, convenient, and suitable for therapeutic monitoring of the nine analytes. 相似文献
16.
A sensitive and rapid liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) method has been developed
and validated for the determination of mizolastine in human plasma using dipyridamole as the internal standard (I.S.). Plasma
samples were simply pretreated with methanol for deproteinization. Chromatographic separation was performed on an Agilent
Zorbax C18 column with a mobile phase of 10 mM ammonium acetate buffer containing 0.1% formic acid–methanol (20:80, v/v) at a flow rate of 1 mL min−1. The electrospray ionization (ESI) interface was employed in a single quadrupole mass spectrometer. The analytes were protonated
in the positive ESI interface and detected in single ion monitoring (SIM) mode. Chromatographic separation was achieved in
less than 3.5 min. The linearity was established over the range of 0.5–600 ng mL−1. The lower limited of quantification (LLOQ) of the method was 0.5 ng mL−1. The intra- and inter-run standard deviations were both less than 11.2%. The method was applied to study the pharmacokinetics
of the mizolastine sustained-release tablets in healthy volunteers. 相似文献
17.
Viñas P López-García I Bravo-Bravo M Briceño M Hernández-Córdoba M 《Analytical and bioanalytical chemistry》2012,403(4):1059-1066
A miniaturized dispersive liquid–liquid microextraction (DLLME) procedure coupled to liquid chromatography (LC) with fluorimetric
detection was evaluated for the preconcentration and determination of thiamine (vitamin B1). Derivatization was carried out by chemical oxidation of thiamine with 5 × 10−5 M ferricyanide at pH 13 to form fluorescent thiochrome. For DLLME, 0.5 mL of acetonitrile (dispersing solvent) containing
90 μL of tetrachloroethane (extraction solvent) was rapidly injected into 10 mL of sample solution containing the derivatized
thiochrome and 24% (w/v) sodium chloride, thereby forming a cloudy solution. Phase separation was carried out by centrifugation, and a volume of
20 μL of the sedimented phase was submitted to LC. The mobile phase was a mixture of a 90% (v/v) 10 mM KH2PO4 (pH 7) solution and 10% (v/v) acetonitrile at 1 mL min−1. An amide-based stationary phase involving a ligand with amide groups and the endcapping of trimethylsilyl was used. Specificity,
linearity, precision, recovery, and sensitivity were satisfactory. Calibration graph was carried out by the standard additions
method and was linear between 1 and 10 ng mL−1. The detection limit was 0.09 ng mL−1. The selectivity of the method was judged from the absence of interfering peaks at the thiamine elution time for blank chromatograms
of unspiked samples. A relative standard deviation of 3.2% was obtained for a standard solution containing thiamine at 5 ng mL−1. The esters thiamine monophosphate and thiamine pyrophosphate can also be determined by submitting the sample to successive
acid and enzymatic treatments. The method was applied to the determination of thiamine in different foods such as beer, brewer’s
yeast, honey, and baby foods including infant formulas, fermented milk, cereals, and purees. For the analysis of solid samples,
a previous extraction step was applied based on an acid hydrolysis with trichloroacetic acid. The reliability of the procedure
was checked by analyzing a certified reference material, pig’s liver (CRM 487). The value obtained was 8.76 ± 0.2 μg g−1 thiamine, which is in excellent agreement with the certified value, 8.6 ± 1.1 μg g−1. 相似文献
18.
Lei Zhang Liang Xu Xiao-Jie Tan Qiong-Feng Liao Wei Guo Xiao-Hui Chen Kai-Shun Bi 《Chromatographia》2007,66(1-2):115-120
A sensitive and reliable ion-paired high-performance liquid chromatographic method has been established for the simultaneous
quantification of six major active ingredients, namely baicalin, baicalein, wogonin, oxysophocarpine, oxymatrine and matrine
in the Chinese herbal preparation, Sanwu-Huangqin-Tang. HPLC analyses were performed on a Phenomenex luna C18 column with mobile phase of methanol–acetonitrile–aqueous phosphoric acid at a flow rate of 0.9 mL min−1. The complete separation was achieved within 35 min for the six target constituents. A good linear regression relationship
between peak-areas and concentrations was obtained over the range of 12.10–242.0 μg*mL−1 for baicalin, 5.05–101.0 μg*mL−1 for baicalein, 0.95–19.0 μg*mL−1 for wogonin, 2.75–55.0 μg*mL−1 for oxysophocarpin, 2.75–55.0 μg*mL−1 for oxymatrine and 4.90–98.0 μg*mL−1 for matrine, respectively. The repeatability was evaluated by intra- and inter-day assays with relative standard deviation
(RSD) being less than 5.1%. The recoveries, measured at three concentration levels, varied from 93.8 to 102.1%. The assay
was successfully applied for determination of six bioactive compounds in Sanwu-Huangqin-Tang. The interaction of chemical
constituents was observed when the herbs were used in compatibility. The results indicated that the developed assay method
was rapid, accurate and could be readily utilized as a quality control method for Sanwu-Huangqin-Tang. 相似文献
19.
Toma Galaon Medeea Radulescu Victor David Andrei Medvedovici 《Central European Journal of Chemistry》2012,10(4):1360-1368
Injectable solutions used in treatment of intense pain are based on combinations of active ingredients such as metamizole sodium (MTZ), pitofenone hydrochloride (PTF) and fenpiverine bromide (FPB). The simultaneous chromatographic assay of such combinations poses difficulties due to their structural variety, highly polar character, and wide concentration ranges (500 mg mL−1 for MTZ, 2 mg mL−1 for PTF and 0.02 mg mL−1 for FPB). Fast hydrolysis of MTZ on aqueous dilution causes additional problems due to impurity (MTC) formation. Sodium hexane sulphonate (10 mM) was used as ion pairing agent for PTF, FPB and MTC in a mobile phase consisting of 48/52 (v/v) methanol and aqueous 0.2% triethylamine at pH=3. The ionic liquid 1-butyl-1-methyl-pyrrolidinium tetrafluoroborate (10 mM) was used as mobile phase additive to preserve the MTZ peak symmetry. The minor active ingredient FPB was selectively extracted into 1-octanol by ion pair formation with picric acid. A 20 μL aliquot of the organic layer was directly injected into the column. 相似文献
20.
Li X Wang G Xie H Wang R Xu M Wang W Tao J Sun J 《Analytical and bioanalytical chemistry》2006,384(4):958-963
A rapid, reproducible high-performance liquid chromatographic (HPLC) method with fluorescence detection for the simultaneous
determination of 3(or 8)-(1-methoxyethyl)-8(or 3)-(1-hydroxyethyl)-deuteroporphyrin IX (MHD) and 3,8-di-(1-methoxyethyl)-deuteroporphyrin
IX (DMD) in dog plasma was described. Fluorescein was used as an internal standard. A simple extraction step with ethyl acetate
was performed before chromatography on a Diamonsil C18 column (5 μm, 4.6 mm×150 mm). The chromatography used 0.02 mol L−1 sodium acetate/tetrahydrofuran (66:34 v/v). The analytical curve was linear over the concentration range 0.025– 2.5 μg mL−1. For a 100 μL dog plasma sample, the limit of determination for both MHD and DMD was 0.025 μg mL−1. The recoveries of MHD and DMD were more than 76% and 89%, respectively. The intra-assay (within-run) and interassay (between-run)
coefficients of variation (precisions) for MHD and DMD were less than 15%. This method was found to be suitable for the analysis
of biosamples and was successfully applied to pharmacokinetic studies of Deuxemether in dogs. 相似文献