Separation and Determination of Bases by Capillary Zone Electrophoresis

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Chiral Separation of Esmolol and Terazosin by Cyclodextrinmodified Capillary Zone Electrophoresis

IntroductionTheincreasingnecessity0fpharmacologicalresearchforchiraldrugshasrenderedchiralseparationanactiveareaincaPillaryelectroph0resis(CE)today.Variousmodes0fCE,includingcaPillaryzoneelectroph0resis(CZE),micellarelectrokineticchromatography(MEKC),caPillarygelelectrophoresis(CGE)andcapillaryisotachophoresis(CITP),havebeenappliedforthispurp0se',amongwhichCZEisthemostpopularoneforitshighefficiency,lowexpenseandeaseofoperation.Forsuccessfulchiralseparati0ninCZE,theselectortypeisofcr…     

Determination of Size Distribution of Nano-particles by Capillary Zone Electrophoresis

A new method was developed for the determination of the size distribution of nano-particles by capillary zone electrophoresis (CZE). Scattering effect of nanoparticles was studied. This method for the determination of size distribution was statistical.     

Enhancement of Selectivity in Capillary Zone Electrophoresis by Adding Ethanolamines or Ethylamines in the Running Buffer

IntroduCtionTheseleedvityandresolutionmCaPilwizoneeleCtrophoresis(CZE)areialluencedbymanyfacors,mcludingbuffercomposition,concentrationandpH,vanousadditives,eldricStrength,temperature,etc.ModulwitheseparametersInayleadtOtheenhancementofseleCtivityandresolution[l].However,optimhationoftheseparameterslsoftentime-consunung.onlydritedpapersinCZEhavedealtwiththeialluenceofcationicspeciesinthebuffertoWarseParationsofar.IsSaqetalI2,3]StUdiedtheeffeCtofvariousalkalineionsinaCetatebufferonthe…     

Evaluation of The Interaction Between Netropsin and Double Stranded DNA by Capillary Zone Electrophoresis

DNAmoleculesarepolymorphic,andexistinavarietyofstructuralformsthatmightprovideuniquebindingsitesforsmallmolecules1.ManysmallmoleculesthatinteractwithDNAhavepotentialtherapeuticapplicationsininhibitingtumorgrowth,andviral,bacterial,fungalandparasiticinfections.Thesedrugscanbedividedintotwocategories:intercalatorandminorgroovebinder2-3.SoDNA-druginteractionsareaparticularlyimportantclassofintermolecularinteractions.ManymethodshavebeenperformedtostudythebasisofDNA-druginteractions4.However,i…     

Enantiomeric Separation and Determination of R,S-Salsolinol by Capillary Electrophoresis

A new method for the quantitative determination of the enantiomers of salsolinol, an endogenous neurotoxin, was reported. Enantiomeric separation of salsolinol was obtained by capillary zone electrophoresis using hydroxypropyl-β-cyclodextrin （HP-β-CD） as chiral selector. Coupled with UV detection, this separation was applied to the determination of R,S-salsolinol in dried banana chips, and both R- and S-salsolinol were found at 40 ktg/g level.     

Chiral Separation of N-Benzoyl Phenylalanine Methyl Ester by Nonaqueous Capillary Electrophoresis

Theseparationofopticalisomersisarapidlygrowingareaincapillaryelectrophoresis(CE),andmostchiralseparationbyCEhavebeenperformedinaqueousbackgroundelectrolyte.Organicsolventssuchasmethanolandacetonitrilehavebeenappliedasbuffermodifiersatconcentrationtypicallynothigherthan40%inordertoincreasehydrophobicanalytesolubilityandtoimproveselectivity,resolution,ortoaltertheelectroosmoticflow(EOF)andelectrophoreticmobilityofanalyte.Buttherehavebeenonlyafewreportsonthechiralseparationusingpurenonaqueousme…     

Characterization of the Interaction between Bovine Serum Albumin and Lomefloxacin by Capillary Zone Electrophoresis

Three capillary zone electrophoresis (CZE) methods of the frontal analysis (FA), vacancypeak (VP) and simplified Hummel-Dreyer (SHD) were applied to investigate interaction betweenbovine serum albumin (BSA) and lomefloxacin, the experimental condition was established after alarge number of tests. Based on the site-binding model, the binding parameters were measuredaccording to the site model by Scatchard.     

Assay of Histamine in Single Mast Cells by Capillary Zone Electrophoresis with Electrochemical Detection

Histamine is one of the chemical mediators in connection with allergies. Only Pihel et al.1 reported a method for detection of histamine in isolated mast cells by high- performance liquid chromatography with electrochemical detection. In this method, single cells were removed and transferred to 300-nL microvials. A part of the supernatant from individual vials was injected into the chromatography column. The analysis of histamine in individual rat peritoneal mast cells using CZE with amp…     

Improvement of Selectivity with Boric Acid in Separating Phenolic Acids by Capillary Zone Electrophoresis

IntroductionTilersarevariousorgamcacidsintheextractsfromChineseadditionalplantmedicines.1Manyphenolicacids,suchasferulicacid,clllorogenicacid.vanillicacid,phydroxybenzoicacid,gallicacid,andprotocatechulcacidarefrequentlymet.AnalysisoftheseacidscanhelpinevaluatingalequalityoftileInedicine.CEisarecentlydevelopedtechniqueclmcterizedwithhighseparahonefficiency,shortanalysistime,lesssampleconsumption,minimalsamplepretrChtlnent,lowcost,andeaseofmodechange-overandcolumnregenefation.Studiesintillsar…     

A Novel Method for Quantification of Circulating DNA in Serum by Capillary Zone Electrophoresis

In this paper, we first presented a novel method for quantification of circulating DNA in human serum based on capillary zone electrophoresis with laser-induced fluorescence detection (CZE-LIF). The serum was digested by proteinase to release free DNA, and then CZE-LIF system was used for the quantification of total circulating DNA. This method was successfully used to quantify the circulating DNA levels in sera from healthy individuals and certain cancer patients.We found the significantly elevated circulating DNA levels in certain prostate cancer patients. Our results demonstrated that CZE-LIF system has good linearity, excellent sensitivity (0.5 ng/mL DNA),satisfactory reproducibility (RSDs in one day and between days were both less than 5%) and reliability, and is well suitable to the quantification of the circulating DNA in human serum or plasma.     

On-line Separation and Detection of Peptides by Capillary Electrophoresis / Electrospray FT-ICR-MS

Recently, capillary electrophoresis/electrospray ionization mass spectrometry (CE/ESI- MS) has been rapidly developed as a powerful analytical tool for charged species ranged from small molecules such as carboxylic acids1, phenolic compounds2, metal species3, tetramines4, herbicides5, drugs and drug metabolites6 to peptides and proteins7, 8. The ESI mode has proven to be sensitive, versatile and relatively easy to use in combination with CE. CE confers rapid analysis and efficient separ…     

Optimized Separation of Pharmacologically Active Xanthones from Securidaca inappendiculata by Capillary Electrophoresis

Securidaca inappendiculata Hassk. is a traditional Chinese herbal medicine. Pharma- cological investigations have shown that the xanthones, as main components accumulated in S. inappendiculata have many bioactivities, such as monoamine oxidase inhibition, antitumor activity, cytotoxicity, antibacterial activity, antifungal activity, anti-inflammatory properties, antioxidant activity and tuberculoatatic activity, etc1. The contents of xanthones are very important and key factors for quality …     

On-line Separation and Detection of Peptides by Capillary Electrophoresis/Electrospary FT-ICR-MS

The coupling of capillary electrophoresis to electrospray Fourier transfrom ion cyclotron resonance mass spectrometry (FT-ICR-MS) was presented for the on-line separation and detection of mixture of peptides.With the ultra-high resolution and unmatchable mass measurement accuracy FT-ICR-MS provides,the separation and identification of angiotension Ⅲ,octreotide and elastin chemotactic were successfully achieved using this fabricated device,which showed its great application potential in detection and structural identification for multicomponent sample.     

Optimized Separation of Pharmacologically Active Xanthones from Secuidaca inappendiculata by Capillary Electrophoresis

A capillary electrophoresis (CE) method has been firstly used for the separation of the therapeutically important xanthones from Securidaca inappendiculata. The separation of the nine xanthones was systematically optimized with respect to pH, concentration of running buffers,addition of sulfated β-CD,applied voltage and column temperature.Baseline separation was achieved for the nine xanthones in less than 15 minutes using a background electrolyte consisting of 200 mmol/L borate (pH9.5) and 10 mmol/L sulfated β-CD.     

Studies on Immunoglobulin G in Human Sera from Guillain Barre Syndrome Patients (Ⅰ)——The Separation of Immunoglobulin G by Capillary Zone Electrophoresis

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Analysis of Phenolic Compounds in Coke Plant Wastewater by Capillary Zone Electrophoresis with Inhibited Chemiluminescence Detection

Phenol and benzenediols are often present together in raw materials in various chemical industries, biological and environmental samples1. It is therefore important to develop a highly sensitive and selective analytical method for the determination of the…     

Molecularly Imprinted Polymer Monolithic Column Separation of Isomers and Analogues of Vanillin by Capillary Electrochromatography

A vanillin imprinted capillary monolithic column was synthesized by in situ polymerization reaction using ethylene-glycol dimethacrylate as cross-linking monomer and methacrylic acid as functional monomer. Under the optimum conditions of capillary electrochromatography, this molecularly imprinted polymer （MIP）-based column showed high selectivity and could recognize not only template molecule vanillin but also positional isomer o-vanillin from their structural analogues.     

Analysis of Chlorogenic Acid in Herbal Medicines by Capillary Electrophoresis

Atpresent,thecommonlyusedmethodforanalyzingherbalmedicinesishighperformanceliquidchromatography(HPLC).However,theuseofHPLCisrestrictedformanyreasons.Themostseriousproblemisthatthechromatographiccolumniseasilycontaminated.ComparedwithHPLC,capillaryelectrophoresis(CE)ischaracterizedwithhighefficiency,rapidity,lowcostandmultiplemodestobechosen.Inaddition,thecapillarycanbeeasilyregenerated.SoCEisidealforanalyzing"dirty"samples,suchasChineseherbalmedicines.Infact,studiesintheareaarebecoming…