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1.
Matioli Graciette Zanin Gisella M. de Moraes Flávio F. 《Applied biochemistry and biotechnology》2000,84(1-9):955-962
The production of cyclodextrins (CDs) by cyclodextrin-glycosyl-transferase (CGTase) from Bacillus firmus was studied, with respect to the effect of the source of starch upon CD yield and on the selectivity for producing γ-CD.
Cyclodextrin production tests were run for 24 h at 50°C, pH 8.0, and 1 mg/L of CGTase, and substrates were maltodextrin or
the starches of rice, potato, cassava, and corn hydrolyzed up to D. E. 10. Cornstarch was the best substrate for producing
γ-CD. Later, glycyrrhizin (2.5% [w/v]), which forms a stable complex with γ-CD, was added to the cornstarch reaction medium
and increased the yield of γ-CD to about four times that produced with only maltodextrin, but the total yield of CDs remained
practically unchanged. Therefore, the results showed that the studied CGTase is capable of giving relatively high yield of
γ-CD in the presence of glycyrrhizin as complexant and cornstarch as substrate. 相似文献
2.
Matioli Graciette Zanin Gisella M. De Moraes Flávio F. 《Applied biochemistry and biotechnology》2001,91(1-9):643-654
The enzyme cyclod extringly cosyltransferase (CGTase), EC2.4.1.19, which produces cyclodextrins (CDs) from starch, was obtained
from Bacillus firmus strain no. 37 isolated from Brazilian soil and characterized in the soluble form using as substrate 100 g/L of maltodextrin
in 0.05 M Tris-HCl buffer, 5 mM CaCl2, and appropriate buffers. Enzymatic activity and its activation energy were determined as a function of temperature and pH.
The activation energy for the production of β- and γ-CD was 7.5 and 9.9 kcal/mol, respectively. The energy of deactivation
was 39 kcal/mol. The enzyme showed little thermal deactivation in the temperature range of 35–60°C, and Arrhenius-type equations
were obtained for calculating the activity, deactivation, and half-life as a function of temperature. The molecular weight
of the enzyme was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis, giving 77.6k Da. Results for CGTase
activity as a function of temperature gave maximal activity for the production of β-CD at 65°C, pH 6.0, and 7 1.5 mmol of
β-CD/(min·mg of protein), whereas for γ-CD it was 9.1 m mol of γ-CD/(min·mg of protein) at 70°C and pH 8.0. For long contact
times, the bestuse of the enzymatic activity occurs at 60°C oratalower temperature, and the reaction pH may be selected to
increase the vield of a desired CD. 相似文献
3.
Arpansiree Wongmekiat Satoko Yoshimatsu Yuichi Tozuka Kunikazu Moribe Keiji Yamamoto 《Journal of inclusion phenomena and macrocyclic chemistry》2006,56(1-2):29-32
Drugs with poor water solubility were co-ground with cyclodextrins (CDs) to create nanoparticles with improved solubility characteristics. Indomethacin (IDM), furosemide (FRM) and naproxen (NAP) were co-ground with β-CD at the molar ratio of 2:1 (CD:drug). Co-grinding of a drug with CD resulted in not only the formation of drug nanoparticles but also the solubilization of the drug by inclusion complex formation with CD in aqueous media. The nanoparticle fraction of IDM, and FRM from ground mixtures prepared with β-CD was as high as 60–70% while the solubilization fraction was less than 10%. In contrast, β-CD–NAP ground mixture showed a large fraction, 48%, for drug solubilization and only 4% for nanoparticle formation. Furosemide ground mixtures prepared with α-CD, β-CD and γ-CD showed comparatively high nanoparticle fraction while the solubilization fraction was around 10%. Both the nanoparticle fraction and the solubilization fraction were greater in the IDM–β-CD system than those in γ-CD and α-CD systems. The nanoparticle formation of NAP depended on the types of CD used as a co-grinding additive. Naproxen nanoparticles could be prepared by co-grinding NAP and α-CD, while the solubilization of NAP tended to improve when β-CD or γ-CD was used. 相似文献
4.
Pekka Mattssoin Tlmo Korpela Sari Paavilainen Mauri Mäkelä 《Applied biochemistry and biotechnology》1991,30(1):17-28
Improved formation of cyclodextrins (CDs) from starch in ethanolic solutions byBacillus circulans var alkalophilus cyclomaltodextrin glucanotransferase was studied. The β- and γ-CD yields increased and α-CD yield gradually decreased as
the ethanol concentration was raised. The ethanol concentration required for maximal CD yield depended essentially on starch
concentration. The ethanol's effect was pronounced at high starch concentrations. For example, with 30% (w/v) starch, the
CD yield was 2.4-fold (146.5 g/L) in the presence of 15% (v/v) ethanol. The effect of dimethylsulfoxide on the formation of
CDs was similar to that of ethanol. The disintegration of β- and γ-CDs were narrowly interdependent on the formation of a
α-CD and malto-sugars. The amount of reducing sugars decreased from a dextrose equivalent value of roughly 7.5 to 4.5 in the
presence of ethanol at starch concentrations 1-30% (w/v). The effect of ethanol on starchy materials from various sources
was similar. It was concluded that ethanol retards the decomposition of β-CD by a general mechanism involving a decreased
activity of water. 相似文献
5.
H. Krajian N. Mofaddel D. Villemin P. L. Desbène 《Analytical and bioanalytical chemistry》2009,394(8):2193-2201
Enantioseparation of 6,6′-dibromo-1,1′-binaphthyl-2,2′-diol (DBBD) by cyclodextrin-modified capillary zone electrophoresis
(CD-CZE) was studied using the three native α, β, and γ cyclodextrins, the three hydroxypropylated cyclodextrins (2-hydroxypropyl-α,
β, and γ), heptakis-2,6-di-O-methyl-β-CD (DM-β-CD), and heptakis-2,3,6-tri-O-methyl-β-cyclodextrin (TM-β-CD). First, the acidity constants of DBBD were determined using capillary electrophoresis, before
performing enantioseparation. The influence of the concentrations of the studied cyclodextrins on the enantioseparation was
explored and the experimental optimal concentrations were determined and compared to the theoretical optimal concentrations.
Moreover, the apparent complexation constants between each studied cyclodextrin and the two DBBD enantiomers were evaluated
using a non-linear curve fitting method and three linear plotting methods (x-reciprocal, y-reciprocal and double reciprocal). For TM-β-CD, the order of migration of the enantiomers of DBBD reversed as a function
of TM-β-CD concentration. The influence of the nature of methylated cyclodextrin derivatives (methyl-β-CD (M-β-CD) and DM-β-CD)
was then studied. Inversion of the order of migration of the enantiomers of DBBD was observed for DM-β-CD, whereas the S enantiomer of DBBD always migrated first for M-β-CD. 相似文献
6.
Binary Systems of Nifedipine And Various Cyclodextrins in The Solid State. Thermal,FTIR, XRD studies
Brown M. E. Glass B. D. Worthington M. S. 《Journal of Thermal Analysis and Calorimetry》2002,68(2):631-646
Nifedipine complexes with β-cyclodextrin (β-CD), γ-cyclodextrin (γ-CD), 2-hydroxypropyl-β-cyclodextrin (2HP-β-CD), randomly
methylated-β-cyclodextrin (RM-β-CD) and heptakis(2,6-O-dimethyl)-β-cyclodextrin (DM-β-CD) have been prepared by both kneading and heating methods and their behaviour studied by
differential scanning calorimetry (DSC), diffuse reflectance mid-infrared spectroscopy (FTIR) and X-ray diffractometry (XRD).
DSC revealed the nifedipine melting endotherm with onset at approximately 171°C for the kneaded mixtures with β-CD, γ-CD and
2HP-β-CD, thus confirming the presence of nifedipine in the crystalline state, while some decrease in crystallinity was observed
in the DM-β-CD kneaded mixture. With RM-β-CD, however, broadening and shifting of the nifedipine endotherm and reduction in
its intensity suggested that the kneading could have produced an amorphous inclusion complex. These differing extents of interaction
of nifedipine with the cyclodextrins were confirmed by FTIR and XRD studies.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
7.
Xiao-Xiang Li Jun-Wen Wang Yu-Jing Guo Ling-Hong Kong Jing-Hao Pan 《Journal of inclusion phenomena and macrocyclic chemistry》2007,58(3-4):307-315
In phosphate buffer solution of pH5.4, the interaction of meso-tetrakis(2-thienyl)porphyrin(H2TTP) and Cu-meso-tetrakis(2-thienyl)porphyrin(Cu-TTP) with α-cyclodextrin(α-CD), β-CD, γ-CD, heptakis(2,3,6-tri-O-methyl)-β-CD(TM-β-CD)
has been studied by means of UV-vis, fluorescence and 1HNMR spectroscopy, respectively. The H2TTP and Cu-TTP can form 1:2 inclusion complexes with TM-β-CD and 1:1 inclusion complexes with the other three cyclodextrins.
In this paper, the inclusion constants (K) of H2TTP and Cu-TTP for the formation of the inclusion complexes have been estimated from the changes of absorbance and fluorescence
intensity in phosphate buffer solution. The inclusive capabilities of different kinds of cyclodextrins are compared. The result
shows that the inclusion ability of α-CD with H2TTP and Cu-TTP is the strongest among the three native CDs. The inclusion ability of modified β-CD with H2TTP and Cu-TTP is stronger, compared to the native β-CD, which indicates that the capacity matching plays a crucial role in
the inclusion procedure except for the hydrophobic effect. In addition 1HNMR spectra supports the inclusion conformation of the TM-β-CD-Cu-TTP inclusion complex, indicating the interaction mechanism
of inclusion processes. 相似文献
8.
Tardioli Paulo W. Zanin Gisella M. de Moraes Flávio F. 《Applied biochemistry and biotechnology》2000,84(1-9):1003-1019
Cyclodextrin-glycosyl-transferase (EC2.4.1.19), produced by Wacker (Munich, Germany), was purified by biospecific affinity
chromatography with β-cyclodextrin (β-CD) as ligand, and immobilized into controlled pore silica particles (0.42 mm). This
immobilized enzyme (IE) had 4.7 mg of protein/g of support and a specific activity of 8.6 μmol of β-CD/(min·gIF) at 50°C, pH 8.0. It was used in a fluidized-bed reactor (FBR) at the same conditions for producing cyclodextrins (CDs) with
10% (w/v) maltodextrin solution as substrate. Bed expansion was modeled by the Richardson and Zaki equation, giving a good
fit in two distin ctranges of bed porosities. The minimum fluidization velocity was 0.045 cm/s, the bed expansion coefficient
was 3.98, and the particle terminal velocity was 2.4 cm/s. The FBR achieved high productivity, reaching in only 4 min of residence
time the same amount of CDs normally achieved in a batch reactor with free enzyme after 24h of reaction, namely, 10.4 mM β-CD
and 2.3 mM γ-CD. 相似文献
9.
Matioli Graciette Zanin Gisella M. De Moraes Flávio F. 《Applied biochemistry and biotechnology》2002,98(1-9):947-961
The influence of substrate or product level on the initial velocity of cyclodextrin (CD) production by cyclodextringlycosyltransferase
from a Brazilian isolate of Bacillus firmus was studied. Our results indicate that the product γ-CD is a stronger inhibitor to the reaction than β-CD. Small saccharides
could also inhibit CD production, although to a lesser extent than the products, and maltose was the strongest inhibitor among
small saccharides. Increasing substrate concentration resulted in greater reduction on enzyme activity for the formation of
β-CD than for γ-CD. We modeled the kinetics of CD production with a set of four reversible reactions including the cyclization/coupling
reaction that forms/opens CDs, and three disproportionation reactions. Our model on the initial velocity data explained well
the substrate inhibition phenomenon. Kinetic parameters were determined by fitting the initial velocity data into our model. 相似文献
10.
Grafting of cyclodextrins onto filter paper 总被引:1,自引:0,他引:1
Rita C. de Bergamasco Gisella M. Zanin Flavio F. de Moraes 《Journal of inclusion phenomena and macrocyclic chemistry》2007,57(1-4):75-78
Grafting of cyclodextrins and cyclodextrins derivatives on cellulosic surface, such as paper or filter paper, provides hosting
cavities that can include a large variety of chemicals for specific cellulose finishing. In this study grafting of monocholorotriazinyl-β-cyclodextrin
(MCT-β-CD) and β-cyclodextrin (β-CD) to filter paper has been performed. β-cyclodextrin has been bonded to filter paper using
1,4-butanediol diglycidyl ether as the crosslinking agent. The untreated and treated filter papers were characterized by differential
scanning calorimetry (DSC) and thermogravimetric analysis (TGA), demonstrating the covalent binding of cyclodextrins to filter
paper. The quantification of β-CD and MCT-β-CD grafted to filter paper was determined by the dye extinction method with the
inclusion of phenolphthalein. The final β-CD content amounted to 15.9 μmol per gram of support (1.8% by weight), and 72.8 μmol
per gram of support (11.3% by weight) for MCT-β-CD. 相似文献
11.
Silvia Simeoni Santo Scalia Rosanna Tursilli Heather Benson 《Journal of inclusion phenomena and macrocyclic chemistry》2006,54(3-4):275-282
The objective of this study was to investigate the influence of cyclodextrins on the cutaneous availability of the sunscreen oxybenzone. The interaction between oxybenzone and hydrophilic α-, β- and γ-cyclodextrin derivatives was studied in water by phase-solubility analysis. Among the available cyclodextrins, hydroxypropyl-β-cyclodextrin (HP-β-CD) and especially sulfobutylether-β-cyclodextrin (SBE-β-CD) had the greatest solubilizing activity. Ethanol–water solutions containing oxybenzone free or complexed with HP-β-CD or SBE-β-CD were applied to human skin in Franz diffusion cells and the amount of sunscreen permeated into the different cutaneous compartments was determined by HPLC. As much as 20.5% of the oxybenzone applied dose diffused within the skin tissue after 6 h application. Between 39.4% and 54.9% of the penetrated UV filter was localized in the stratum corneum, with no significant difference between uncomplexed oxybenzone or its complex with HP-β-CD. Conversely, the amount retained in the stratum corneum was markedly decreased (ca. 50%) by complexation with SBE-β-CD. Considerable quantities of oxybenzone accumulated into the viable epidermis (5.7% of the applied dose) and dermis (6.2% of the applied dose) from the preparation containing the free UV filter. The sunscreen penetration to the deeper living layers of the skin was remarkably lower (1.0% and 2.0% of applied dose for epidermis and dermis, respectively) upon application of the sunscreen complexed with SBE-β-CD, whereas HP-β-CD had no effect. In addition, photostability experiments demonstrated that SBE-β-CD complexation did not alter the sunscreen photochemical properties. 相似文献
12.
Production of Cyclodextrins by CGTase from Bacillus clausii Using Different Starches as Substrates 总被引:1,自引:0,他引:1
Alves-Prado HF Carneiro AA Pavezzi FC Gomes E Boscolo M Franco CM da Silva R 《Applied biochemistry and biotechnology》2008,146(1-3):3-13
Cyclodextrins (CDs) are cyclic oligasaccharides composed by d-glucose monomers joined by α-1,4-d glicosidic linkages. The main types of CDs are α-, β- and γ-CDs consisting of cycles of six, seven, and eight glucose monomers,
respectively. Their ability to form inclusion complexes is the most important characteristic, allowing their wide industrial
application. The physical property of the CD-complexed compound can be altered to improve stability, volatility, solubility,
or bio-availability. The cyclomaltodextrin glucanotransferase (CGTase, EC 2.4.1.19) is an enzyme capable of converting starch
into CD molecules. In this work, the CGTase produced by Bacillus clausii strain E16 was used to produce CD from maltodextrin and different starches (commercial soluble starch, corn, cassava, sweet
potato, and waxy corn starches) as substrates. It was observed that the substrate sources influence the kind of CD obtained
and that this CGTase displays a β-CGTase action, presenting a better conversion of soluble starch at 1.0%, of which 80% was
converted in CDs. The ratio of total CD produced was 0:0.89:0.11 for α/β/γ. It was also observed that root and tuber starches
were more accessible to CGTase action than seed starch under the studied conditions. 相似文献
13.
A. A. Abdoh M. B. Zughul J. Eric. D. Davies A. A. Badwan 《Journal of inclusion phenomena and macrocyclic chemistry》2007,57(1-4):503-510
Guest–host interactions were examined for neutral diclofenac (Diclo) and Diclofenac sodium (Diclo sodium) with each of the
cyclodextrin (CD) derivatives: α-CD, β-CD, γ-CD and 2-hydroxypropyl-β-cyclodextrin (HP-β-CD), all in 0.05 M aqueous phosphate
buffer solution adjusted to 0.2 M ionic strength with NaCl at 20 °C, and with β-CD at different pHs and temperatures. The
pH solubility profiles were measured to obtain the acid–base ionization constants (pK
as) for Diclo in the presence and absence of β-CD. Phase solubility diagrams (PSDs) were also measured and analyzed through
rigorous procedures to obtain estimates of the complex formation constants for Diclo/CD and Diclo sodium/CD complexation in
aqueous solutions. The results indicate that both Diclo and Diclo sodium form soluble 1:1 complexes with α-, β-, and HP-β-CD.
In contrast, Diclo forms soluble 1:1 Diclo/γ-CD complexes, while Diclo sodium forms 1:1 and 2:1 Diclo/γ-CD, but the 1:1 complex
saturates at 5.8 mM γ-CD with a solubility product constant (pK
sp = 5.5). Therefore, though overall complex stabilities were found to follow the decreasing order: γ-CD > HP-β-CD > β-CD > α-CD,
some complex precipitation problems may be faced with aqueous formulations of Diclo sodium with γ-CD, where the overall concentration
of the latter exceeds 5.8 mM γ-CD. Both 1H-NMR spectroscopic and molecular mechanical modeling (MM+) studies of Diclo/β-CD indicate the possible formation of soluble isomeric 1:1 complexes in water. 相似文献
14.
Lima Heron O. S. De Moraes Flavio F. Zanin Gisella M. 《Applied biochemistry and biotechnology》1998,(1):789-804
Production of β-cyclodextrin (CD) with high-dextrose equivalent (DE) starch hydrolysates by simultaneous fermentation and
cyclization (SFC) gives higher yields than using only the enzyme CGTase, because fermentation eliminates glucose and maltose
that inhibit CD production, while at the same time, produces ethanol that increases yield. A 10% (w/v) solution of cassava
starch, liquefied with α-amylase, was incubated with CGTase using: only the enzyme, added ethanol (from 1 to 5%), and added
yeast,S. cerevisiae (12% w/v), plus nutrients, the latter being the SFC process. Reaction conditions were: 38αC, pH 6.0, DE from 2 to 25, and
3.3 mL of CGTase/L. The yield of β-CD has decreased with an increase in DE, and maximum reaction yields were found for DE
equal to 3.54, reaching 5.6, 14.7, and 11.5 mM β-CD, respectively. For an increase of DE, of approx 6 times (from 3.54 to
23.79), β-CD yield decreased 6 times for the first, and second reaction media with 3% (v/v) ethanol, and only approx 3 times
for SFC (from 11.5 to 3.73 mM), showing that this process is less sensitive to variations in the DE 相似文献
15.
Mahmoud M. Al Omari Musa I. El-Barghouthi Mohammad B. Zughul J. Eric D. Davies Adnan A. Badwan 《Journal of solution chemistry》2008,37(2):249-264
The interaction of two benzocycloheptanes namely, pizotifen (Pizo) and ketotifen (Keto), with cyclodextrins (CDs: α-, β-, γ-, and HP-β-CDs) has been investigated by several techniques including phase solubility, X-ray powder diffractometry, 1H-nuclear magnetic resonance and molecular mechanical modeling. The effects of CD type, pH, ionic strength and temperature
on complex stability were also explored. The complex formation constant (K
11) values for the Pizo/CD system follows the decreasing order β-CD > γ-CD > HP-β-CD > α-CD. However, for the Keto/CD system it follows the decreasing order γ-CD > β-CD > HP-β-CD > α-CD. The tendency of Pizo and Keto to complex with β-CD is driven to the extent of 70% by the hydrophobic effect. Complex formation of Keto and Pizo was substantially driven
by entropy (>100 J⋅mol−1⋅K−1) but slightly retarded by enthalpy (3–8 kJ⋅mol−1). 1H-NMR and MM+ studies indicate multimodal inclusion of the methylpiperadine, thiophene and phenyl moieties into the β-CD cavity. 相似文献
16.
Summary
Bacillus circulans E 192 Cyclodextrin-glucosyltransferase was purified 40 fold to homogeneity on a large scale, by affinity chromatogrpahy on
a β-CD copolymer cross-linked with epichlorhydrin. A preliminary step was necessary to remove maltooligosaccharides and cyclomaltooligosaccharides
from the crude extract. The simplest method consists in performing the affinity chromatography directly from a redissolved
65% saturation ammonium sulfate precipitate of the crude extract. Five successive affinity runs of Cetavlon — treated concentrated
extract gave about one gram of pure CGTase with a 70% yield, using 50 g of a 750 U/g β-CD copolymer. This rapid purification
is described and the advantages of the method are discussed. 相似文献
17.
Sanyo Hamai 《Journal of inclusion phenomena and macrocyclic chemistry》2007,58(3-4):241-247
In pH 7.3 buffers, the interactions of a cationic porphyrin, tetrakis(4-N-methylpyridyl)porphyrin (TMPyP), with cyclodextrins (CDs) and disodium phthalate (DSP) have been examined by means of absorption,
fluorescence, and induced circular dichroism spectroscopy. α-CD, β-CD, and γ-CD form a 1:1 inclusion complex with a TMPyP
monomer, which dimerizes in solution without CD. TMPyP also forms a 1:1 organic cation–organic anion complex with DSP. The
1:1 TMPyP–DSP complex forms a ternary CD–TMPyP–DSP inclusion complex with α-, β-, and γ-CD, in which a DSP molecule is not
incorporated into the CD cavity. From the fluorescence intensity change, the␣equilibrium constants have been evaluated for
the formation of the inclusion complexes and the organic cation–anion complexes. 相似文献
18.
De-zhi Sun Ling Li Xiao-mei Qiu Min Liu Bao-lin Yin 《Journal of solution chemistry》2006,35(11):1537-1549
Interactions between CDs with three substituted phenols, paeonol (Pae), acetovanillone (Ace) and 2-hydroxyl-5-methoxy-acetophone (Hma), which are isomers, have been determined by isothermal titration calorimetry (ITC) and 1H NMR in aqueous solution at 298.2 K. Both the binding thermodynamics and 1H NMR spectra show that the interaction between α-cyclodextrin (α-CD) molecule and each guest molecule is extremely weak. The thermodynamic parameters indicate that the binding processes of β-cyclodextrin (β-CD) with the isomers are mainly entropy driven and that β-CD binds with Pae or Ace in 1:1 stoichiometry, whereas with Hma binds in 1:1 and 2:1 stoichiometries. The thermodynamic parameters also suggest that γ-cyclodextrin (γ-CD) binds each isomer in the same 1:1 stoichiometry. The binding processes of Pae and Hma with γ-CD are enthalpy driven whereas Ace with γ-CD is predominantly driven by entropy. The 1H NMR spectra reveal that the three isomers were trapped into the torus cavity of the β-CD molecule from the narrow side during the binding process. Pae penetrates into the γ-CD cavity from the primary rim of the macrocycle whereas Ace does so from the secondary rim, but Hma appears not interact with the internal cavity of γ-CD at all. 相似文献
19.
Yingjin liu Naoto Horiuchi Yoshimi Sueishi Shunzo Yamamoto 《Journal of inclusion phenomena and macrocyclic chemistry》2006,54(3-4):233-239
The kinetics of the hydride transfer reaction between Methylene Blue (MB+) and 1-benzyl-1,4-dihydronicotinamide (BNAH) were studied in 10% ethanol-90% water mixed solvents containing β- and γ-cyclodextrins (β-CD and γ-CD). The pseudo-first order rate constant shows kinetic saturation at high initial concentration of BNAH. This indicates the formation of a complex between MB+ and BNAH. The reaction was suppressed by addition of β-CD, but enhanced by addition of γ-CD. MB+ and BNAH were separately accommodated within the β-CD cavity and the cavity walls may protect the activity site of the reactants. On the other hand, in the MB+-BNAH-γ-CD system, the inclusion of the complex between MB+ and BNAH with γ-CD occurred. This effect of γ-CD can distinguish between the productive and non-productive nature of the complex. 相似文献
20.
Several host–guest inclusion compounds of eugenol as a guest molecule and cyclodextrins (α-,β-,γ-CD) and heptakis (2,6-di-O-methyl)-β-cyclodextrin (DMβ-CD) as hosts were investigated in the solid state and in aqueous solution. The one-to-one solid inclusion compounds of eugenol
and β-CD or γ-CD were prepared, but those of eugenol with α- or DMβ-CD were not obtained under the same condition. However, the UV-visible absorption spectroscopy data indicated that the liquid
guest could form a 1:1 inclusion compound with all four hosts respectively in aqueous solution. The two solid inclusion compounds
were characterized by powder X-ray diffraction (XRD), infrared spectroscopy (IR), thermogravimetric analysis (TG), differential
scanning calorimetry (DSC) and nuclear magnetic resonance (NMR). The association constants (K), calculated from the modified Benesi–Hidebrand equation, of eugenol with α-, β-, γ- and DMβ-CD is 4.95 × 104, 3.96 × 105, 1.47 × 105 and 9.33 × 104 mol−1 dm3, respectively. 相似文献