meso—四（4—溴苯基）卟啉与Cd^2＋的显色反应的研究

研究了Tween—80存在时,非水溶性meso—四(—4—溴苯基)卟啉(TBrPP)与镉络合显色反应的条件以及对络合物光敏性的机理做了一些初步探讨。络合物摩尔组成比为1:1,ε′为4.5×10~5,镉量在0～0.36μg·ml~(-1)有较好的线性关系,线性回归方程A=0.00034+0.161C,相关系数为0.9997,稳定常数为2.6×10~7,用于烟草、茶叶、环境分析标准水样中痕量镉的测定,结果满意。     

儿童常用蜡笔中可溶性铅、镉的测定

采用火焰原子吸收光谱法测定了儿童常用蜡笔中可溶性铅、镉的含量。铅在0.05～10.00μg·mL~(-1)之间,镉在0.02～4.00μg·mL~(-1)之间具有良好的线性关系。方法的RSD≤4.13％。回收率在93.7％～104.5％之间,测定快速简便,结果准确。可用于蜡笔中可溶性铅、镉含量的监测。     

流动注射化学发光法测定食品中叔丁基茴香醚与2,6-二叔丁基对甲酚

基于叔丁基茴香醚(BHA)与2,6 二叔丁基对甲酚(BHT)与铬(Ⅵ)的还原反应,产生的铬(Ⅲ)催化Luminol(鲁米诺) 过氧化氢化学发光体系的研究,结合流动注射技术,建立了一种高灵敏度的快速测定BHA与BHT的新方法。方法的线性范围为2.0×10-9～4.0×10-5g·ml-1和2.8×10-9～1.0×10-5g·ml-1,检出限为6.0×10-10g·ml-1和8.0×10-10g·ml-1,相对标准偏差为1.2%和1.4%(1.0×10-7g·ml-1BHA和BHT,n=11)。方法应用于食品中痕量BHA和BHT的测定,结果满意。     

土壤中水溶性NH4^＋的吸光光度测定

推荐了一个测定土壤中水溶性NH_4~+的灵敏的吸光光度方法。方法基于NH_4~+与水杨酸在次氯酸钠和亚硝基铁氰化钠存在下形成一种有色配合物的反应。最大吸收波长为698nm,体系中NH_4~+浓度在0～1μg·ml~(-1)范围内符合比耳定律,测得摩尔吸光系数ε=1.4×10~4,Sandell灵敏度为1.0×10~(-3)μg·cm~(-2)。可见方法灵敏度高,因此可应用于土壤中痕量NH_4~+的直接吸光光度法测定。     

meso-四(4-磺酸基-1-萘基)卟林的合成及其与Cd(Ⅱ)显色反应研究

合成了meso-四(4-磺酸基-1-萘基)卟啉.用元素分析和红外光谱证明了产物的结构,以光度法测定了试剂在水溶液中的离解常数.在pH=7.0的六次甲基四胺的缓冲溶液中.痕量镉与试剂形成1:2的黄绿色配合物,λ_(max)=440nm,表观摩尔吸光系数ε=4.51×10~5L·mol~(-1)·cm~(-1).Cd(Ⅱ)的浓度在0～0.14μg·ml~(-1)内符合比耳定律.     

二溴羧基苯基重氮氨基偶氮苯的合成及其与镉显色反应的研究

本文首次合成了二溴邻羧基苯基重氮氨基偶氮苯(DB-o-CDAA)和二溴对羧基苯基重氮氨基偶氮苯(DB-p-CDAA)。在pH10.2的硼砂-氢氧化钠缓冲介质中,以TritonX-100为增溶增敏剂,两种试剂均可与Cd(Ⅰ)生成稳定的橙红色络合物。其DB-o-CDAA-Cd,λ_(max)=520nm,ε_(520)=2.21×10~5L·mol~(-1)·cm~(-1);DB-p-CDAA-Cd,λ_(max)=508nm,ε_(508)=1.12×10~5L·mol~(-1)·cm~(-1)。镉量在0～10μg/25ml范围内遵守比耳定律。用这两种试剂测定了环标水样及工业废水中的痕量镉,结果满意。     

硫代米蚩酮-十六烷基三甲基溴化铵胶束增溶分光光度法测定痕量金

本文就十六烷基三甲基溴化铵(CTMAB)对金(Ⅰ)-硫代米蚩酮(TMK)络合物胶束增溶作用进行了试验,发现在pH 2.2—2.8条件下,络合物的灵敏度均比Au(Ⅱ)-TMK在水相中提高一倍左右。测得摩尔吸光系数为1.32×10~5,检出限为0.01μg·mL~(-1):在540—550mn吸收峰最大:0—20μg/25mL符合比尔定律。两年来的生产实践证明,方法具有简便、快速、灵敏度高、重视性好等优点。     

蘑菇组织催化氧化-流动注射荧光法测定去甲肾上腺素和左旋多巴

研究发现富含多酚氧化酶的蘑菇组织可催化溶解氧氧化去甲肾上腺素和左旋多巴在碱性条件下生成具有强荧光的三羟基和二羟基吲哚类物质 ,建立了以蘑菇组织柱为酶反应器的去甲肾上腺素和左旋多巴的流通式荧光分析法 .该法对去甲肾上腺素和左旋多巴响应的线性范围分别为 6× 10 -8～ 1× 10 -5g·mL-1和 3× 10 -8～ 1× 10 -5g·mL-1,检测限分别为 2×10 -8g·mL-1和 1× 10 -8g·mL-1( 3S/k) .该组织反应器可连续使用 14d ,对 1× 10 -7g·mL-1去甲肾上腺素和 1× 10 -7g·mL-1左旋多巴测定的相对标准偏差 (R .S .D .)均小于 3 % (n =11) .详细研究了常见离子和抗氧化剂对本体系的干扰情况 .将该体系用于药物制剂中去甲肾上腺素和左旋多巴含量的测定 ,结果与药典标准方法测得值一致 .实验结果证明了方法的可行性和可靠性     

微波消解-AAS法测定果蔬罐头中镉铅铜锌

采用微波制样技术,用石墨炉和火焰原子吸收法测定果蔬罐头中的镉铅铜锌的四种微量元素.镉、铅、铜、锌的线性范围分别为0～8μg·L~(-1)、0～100μg·L~(-1)、0～4mg·L~(-1)、0～5mg·L~(-1),检出限分别为0.19μg·L~(-1)、2.80μg·L~(-1)、20μg·L~(-1)、60μg·L~(-1),相对标准偏差均小于5%,方法简便、快速,结果准确可靠.     

锗-玫瑰红酸钠-钒(Ⅳ)极谱催化波测定矿石中的锗

本文报导了环己烯二醇钠四酮(玫瑰红酸钠)与锗(Ⅳ)形成的络合物,在钒的存在下,于-0.57伏处(相对于S.C.E)显示灵敏的催化波。当锗浓度为2.4×10~(-(?))—3.2×10~(-1)μg·ml~(-1)时,催化电流与锗量呈     

Furyl- and thienyl-silatranes and germatranes

We review our research on the synthesis and study of the physical and biological properties of furyl- and thienylgermatranes and -silatranes.Translated from Khimiya Geterotsiklicheskikh Soedinenii, No. 6, pp. 725–732, June, 1992.     

Review and patents and literature

The use of the insect cell/baculovirus expression system for producing recombinant proteins of bacterial, plant, insect, and mammalian origin has become widespread. The popularity of this eukaryotic expression system is due to many factors, including (1) potentially high protein expression levels, (2) ease and speed of genetic engineering, (3) ability to accommodate large DNA inserts, (4) protein processing similar to higher eukaryotic cells (e.g., mammalian cells), and (5) ease of insect cell growth (e.g., suspension growth). The following review of the literature discusses two engineering aspects of recombinant protein synthesis by insect cell cultures: bioreactor scale-up and insect cell line selection. Following this review patent abstracts and additional literature pertaining to expression of recombinant proteins in insect cell culture are listed.     

多环芳二酐型聚酯亚胺膜的透气性能

多环芳二酐型聚酯亚胺膜的透气性能李悦生，丁孟贤，徐纪平（浙江大学高分子科学与工程研究所，杭州，３１００２７）（中国科学院长春应用化学研究所）关键词聚醚酰亚胺，聚酯酰亚胺，膜，透气性通常的聚酰亚胺加工性能较差，在芳环二酐的苯环间引入醚键等柔性基团后，其...     

Ground- and excited-state aromaticity and antiaromaticity in benzene and cyclobutadiene

The aromaticity and antiaromaticity of the ground state (S 0), lowest triplet state (T 1), and first singlet excited state (S 1) of benzene, and the ground states (S 0), lowest triplet states (T 1), and the first and second singlet excited states (S 1 and S 2) of square and rectangular cyclobutadiene are assessed using various magnetic criteria including nucleus-independent chemical shifts (NICS), proton shieldings, and magnetic susceptibilities calculated using complete-active-space self-consistent field (CASSCF) wave functions constructed from gauge-including atomic orbitals (GIAOs). These magnetic criteria strongly suggest that, in contrast to the well-known aromaticity of the S 0 state of benzene, the T 1 and S 1 states of this molecule are antiaromatic. In square cyclobutadiene, which is shown to be considerably more antiaromatic than rectangular cyclobutadiene, the magnetic properties of the T 1 and S 1 states allow these to be classified as aromatic. According to the computed magnetic criteria, the T 1 state of rectangular cyclobutadiene is still aromatic, but the S 1 state is antiaromatic, just as the S 2 state of square cyclobutadiene; the S 2 state of rectangular cyclobutadiene is nonaromatic. The results demonstrate that the well-known "triplet aromaticity" of cyclic conjugated hydrocarbons represents a particular case of a broader concept of excited-state aromaticity and antiaromaticity. It is shown that while electronic excitation may lead to increased nuclear shieldings in certain low-lying electronic states, in general its main effect can be expected to be nuclear deshielding, which can be substantial for heavier nuclei.     

微量元素与疾病诊断治疗的研究现状及展望

对国内外微量元素与疾病的关系及其诊断治疗的研究现状及研究动向进行了综述，包括：微量元素与地方病、癌肿、心血管病、糖尿病、眼病、神经系统病等。参考文献72篇。