A new daunorubicin has been synthesized and structurally characterized. The interaction of native calf thymus DNA (ctDNA) with 3′-azido-daunorubicin thiosemicarbazone (ADNRT) was investigated under simulated physiological conditions by multi-spectroscopic techniques, viscometric measurements and molecular modeling study. It concluded that ADNRT could intercalate into the base pairs of ctDNA, and the fluorescence quenching by ctDNA was static quenching type. Thermodynamic parameters calculated suggested that the binding of ADNRT to ctDNA was mainly driven by hydrophobic interactions. The relative viscosity of ctDNA increased with the addition of ADNRT, which confirmed the intercalation mode. Furthermore, molecular modeling studies corroborate the above experimental results. 相似文献
The Raman and infrared spectra of 2,6-diphenyl pyridine, m-terphenyl and 2,2′:6′,2″-1erpyridine have been analysed as metadisubstituted benzene or pyridine molecules. Complete vibrational assignment shows that some modes in the case of internal vibrations of substituents appear in pairs. The splitting of these modes have been attributed to the in-and out-of-phase motions arising out of interaction between phenyl or pyridine rings, as the case may be. In the case of meta terphenyl and terpyridine, two probable cases of Fermi resonance have been observed. 相似文献
3,6-diaryl-2,5-dihydro-1,4-diketopyrrolo[3,4-c]pyrrole-1,4-dione (DPPD) derivatives are used commercially as red pigments,
because of their color strength, brightness, and very low solubility in most common solvents. These products, although highly
colored, appear to be of limited value as dyes, since they show a tendency to form an aggregate when incorporated into a solvent.
Monoalkyl and dialkyl derivatives of DPPD were synthesized and then characterized with IR, 1HNMR, UV-Vis absorption and emission spectroscopy. Molar absorption coefficient, singlet energy level and Stokes’ shift values
of DPPD derivatives were declared. Monoalkyl and dialkyl derivatives of DPPD’s solubilities were measured from saturated solutions
of dimethylformamide (DMF), tetrahydrofuran (THF), dichloromethane (DCM), acetonitrile (ACN) and toluene and they are compared
with each other. 相似文献
Five novel lanthanide (Eu3+, Tb3+, Gd3+, Sm3+ and Dy3+) complexes with 2, 2'-bipyridine-N, N'-dioxide (bipyO2) were synthesized and characterized by elemental analysis, IR spectrum. The triplet state energy of bipy O2 was determined to be 22275 cm(-1) with the phosphorescence spectrum of bipy O2 and its gadolinium complex. The photophysical properties of these complexes indicated that the triplet state energy of the ligand is suitable for the sensitization of the luminescence of Eu3+, Tb3+, Sm3+ and Dy3+, especially of Tb3+. 相似文献
Charge-transfer interactions of perhydroisoquinoline (PHIQ) with 2,3-dichloro-5,6-dicyano-1,4- benzoquinone (DDQ), p-chloranil
(CHL), and 7,7′,8,8′-tetracyanoquinodimethane (TCNQ) in chloroform as a solvent have resulted in stable complexes with a general
formula [(PHIQ)(acceptor)] with a molar ratio of 1:1 (donor:acceptor). Elemental (C, H, N) and thermogravimetric (TGA/DTG)
analyses, photometric titration, and electronic, infrared, and 1H NMR spectra were used to give an idea of the charge-transfer interaction between donating and accepting sites. The Benesi–Hildebrand
method and its modification were used to determine an association constant (K) and a molar extinction coefficient (ε). 相似文献
Abstract In his communication on the detection limits for thermal emission spectroscopy of metallic species in flame media, Fassel (1) attacks our previous statement that detection limits for Al, Be and Mo were available only for turbulent oxygen-supported hydrogen or acetylene flames. It should be borne in mind that he was referring to a preliminary communication (2) which naturally does not carry an extensive bibliography. It should also be noted that we were concerned, in context, with a comparison of the separated flame with conventional flames, not with highly unconventional flames such as those to which he refers. Whilst the point with which he takes issue was a very minor one, incorporated in our preliminary communication mainly as an “aside”, we firmly disagree with his observation. The flame he refers to (3) (4) is, in fact, a diluted oxy-acetylene flame which is supported by venting some air (and therefore nitrogen) to dilute the fuel-oxygen mixture. Fassel's communication (1) erroneously refers to this as a 'premixed oxy-acetylene flame', whereas elsewhere (5) it is described more correctly as an oxygen-nitrogen-acetylene flame. The burner he long-pathlength carbonaceous flames (10). They also allow more sensitive molecular emission spectroscopy of sulphur in an air-hydrogen flame (11), etc. We hope that this unfortunate correspondence will not distract attention from the main issue of our previous or previous preliminary communication on the separated nitrous oxide-acetylene flime. 相似文献
The vibrational frequencies of N‐acetyl‐L‐alanine (NAAL), its potassium salt (NAALK) and its free anionic form (NAAL?) are calculated using density functional theory (B3LYP) combined with the 6‐311 + + G(d,p) basis set. The experimental Raman spectrum of solid NAALK and the scaling factors for calculated values are discussed as well. The three species are characterized by intramolecular NH…O hydrogen bonds leading to the formation of a five‐membered ring. As indicated by the intramolecular (N)H…O distances and by the ν(NH) frequencies, the strength of the intramolecular hydrogen bond is ordered as follows: NAAL? < NAALK < NAAL?. Owing to their difference in the coupling with other vibrational modes, the in‐plane and out‐of‐plane vibrations do not reflect the strength of the hydrogen bond. 相似文献
Novel ESIPT inspired benzimidazole, benzoxazole and benzothiazole were synthesized from 2,4-dihydroxy benzoic acid and 1,2-phenelenediamine, 2-aminophenol, and 2-aminothiophenol respectively. The synthesized 2-(2′,4′-dihydroxyphenyl) benzimidazole, benzoxazole and benzothiazole are fluorescent and the emission characteristic are very sensitive to the micro-environment. They show a single absorption and dual emission with large Stokes shift originating from excited state intramolecular proton transfer. The absorption-emission characteristics of all these compounds are studied as a function of pH. The change in the electronic transition, energy levels, and orbital diagrams of synthesized compounds were investigated by the molecular orbital calculation and were correlated with the experimental spectral emission. Experimental absorption and emission wavelengths are in good agreement with those predicted using the Density Functional Theory (DFT) and Time-Dependent Density Functional Theory (TD-DFT) [B3LYP/6-31G(d)].
Figure
Novel ESIPT inspired benzimidazole, benzoxazole and benzothiazole were synthesized from 2,4-dihydroxy benzoic acid and o- amino aromatics. Single absorption and dual emission are the interesting properties of the synthesized compounds. 相似文献
We have studied1 the protonation behaviour of several cardiotonic polyazaheterocycles and recently shown that the 2-arylimidazo[l,2-a]pyrimidine (3a) undergoes protonation at the imidazo nitrogen (Nl). These investigations have utilised the shielding of acarbon nuclei and increases in 13C-1H or 1H-1H couplings that occur on protonation of a heterocyclic nitrogen. We now report unequivocal protonation studies of the parent heterocycle, imidazo[1,2-a]pyrimidine (l), and its 2-aryl derivative (3b) which have allowed the effect of 2-aryl substitution on protonation to be determined. 15N NMR spectroscopy was employed to determine the protonation site of (1) in a titration study with H2SO4. In addition the N-methyl quaternary salts (21, (4) and (5) were prepared and 15N, 13C and 1H chemical shifts measured so as to provide unambiguous substituent effects uncomplicated by possible proton transfers. 相似文献
Blue emitting cyano substituted isoquinoline dyes were synthesized by one-pot multicomponent reactions (MCRs) of aldehydes, malononitrile, 6-methoxy-1,2,3,4-tetrahydro-naphthalin-1-one and ammonium acetate. Results obtained from spectroscopic (FT-IR, 1H-NMR, 13C-NMR, EI-MS) and elemental analysis of synthesized compounds was in good agreement with their chemical structures. UV–vis and fluorescence spectroscopy measurements proved that all compounds are good absorbent and fluorescent. Fluorescence polarity study demonstrated that these compounds were sensitive to the polarity of the microenvironment provided by different solvents. In addition, spectroscopic and physicochemical parameters, including electronic absorption, excitation coefficient, stokes shift, oscillator strength, transition dipole moment and fluorescence quantum yield were investigated in order to explore the analytical potential of synthesized compounds. The anti-bacterial activity of these compounds were first studied in vitro by the disk diffusion assay against two Gram-positive and two Gram-negative bacteria then the minimum inhibitory concentration (MIC) was determined with the reference of standard drug chloramphenicol. The results displayed that compound 3 was better inhibitors of both types of the bacteria (Gram-positive and Gram-negative) than chloramphenicol.
The modified tris(2,2-bipyridine)ruthenium(II) complex 2 was anchored via a sol-gel process to different polysiloxane matrices to give a series of novel inorganic–organic hybrid polymers. One of the bipyridine ligands of 2 was provided with a long-chain spacer carrying a triethoxysilyl function (T group) at the end, which enables almost free mobility of the transition metal center. The polymers were swollen in several organic solvents of different polarity to investigate the luminescence behavior in the presence of quencher molecules. The luminescence of 2 was quenched using anthracene and atmospheric oxygen in appropriate concentrations of 5 · 10–4 to 5 · 10–3 and 3 · 10–3 M, respectively. The luminescence behavior of 2 was determined by steady-state and time-resolved luminescence experiments. Translational mobilities of molecular species dissolved in the liquid phase were investigated by the kinetics of luminescence quenching after laser excitation. Both the translational mobility of anthracene and atmospheric oxygen, specified with the rate constant k2 and the percentage of accessible luminescent centers were determined. Translational mobilities and the accessibility for anthracene and atmospheric oxygen in hybrid materials are significantly higher than in conventional Q type polysiloxanes. 相似文献
The reactions of π-electron acceptors such as 1-chloro-2,4,6-trinitrobenzene (picryl chloride), 7,7′,8,8′-tetracyanoquino-dimethane (TCNQ), tetrachloro-p-benzoquinone (chloranil) and tetra-cyanoethylene (TCNE) with the cyclic polyamine base 1,4,8,11-tetraazacyclotetradecane (TACTD) have been investigated in CHCl3 solvent. The data indicate the formation of the CT-complexes with the general formula [(TACTD) (acceptor)2]. The 1: 2 stoichiometry of the (TACTD)-acceptor was based on elemental analysis and infrared spectra of the solid CT-complexes along with the photometric titration curves for the reactions in CHCl3. The formation constants (K) for the CT-complexes are shown to be strongly dependent on the type and structure of the acceptor. 相似文献
Four new Hofmanntype complexes, M(DMTF)2Ni(CN)4, (where DMTF is dimethylthioformamide, M=Mn, Cd, Co, or Ni) were synthesized and their structure was determined by an elemental analysis and infrared spectroscopy. The IR spectra of DMTF and its nickel tetracyanine complexes with Mn(II), Cd(II), Co(II), and Ni(II) have been investigated within the range 4000–400 cm–1. The frequency shifts in the metal complexes agree with the assignment of the CS and CN frequencies. The complexes consist of infinite planar polymer layers of |MNi(CN)4|. Ndimethylthioformamide is coordinated to this layer from above and below; it is a monodentate ligand and is Sbonded through the metal atom in these complexes. 相似文献
An increasingly wide variety of fluorescent compounds is used in biotechnology, genomics, immunoassays, array technologies, imaging, and drug discovery. Therefore, synthesis of fluorophores with novel structural features can be interesting and useful in various fields. In this paper, four new fluorescent heterocyclic compounds with high quantum yields are introduced. These new fluorophores are synthesized in moderate to high yields via regioselective nitration of 3-alkyl-8-(4-chlorophenyl)-3 H-imidazo[4′,5′:3,4]benzo[c]isoxazoles. The latter compounds are obtained from the reaction of 1-alkyl-5-nitro-1 H-benzoimidazoles with (4-chlorophenyl)acetonitrile in basic MeOH solution. Physical spectral (UV-vis, IR, 1HNMR, 13C NMR, NOESY and fluorescence) and analytical data have established the structures of synthesized compounds. The fluorescence properties of new fluorescent heterocyclic compounds are studied. The fluorescence of all compounds is very intense and fluorescence quantum yields are high (> 0.52). Density functional theory (DFT) calculations are performed to provide the optimized geometries, relevant frontier orbitals and the prediction of 1H NMR chemical shifts for confirming the exact structure of fluorescent compounds. Calculated electronic absorption spectra were also obtained by time-dependent density functional theory (TD-DFT) method.
4′-O-(α-L-Oleandrosyl)daunorubicin (ODNR) is a disaccharide analogue of daunorubicin with potent antitumor activity against leukemia
cell line K562 cells and colon cancer cell line SW620 cells. In this paper, the binding interaction of ODNR with human serum
albumin (HSA) was investigated under simulative physiological conditions by fluorescence spectroscopy in combination with
UV absorption spectroscopy and molecular modeling method. A strong fluorescence quenching reaction of ODNR to HSA was observed
and the quenching mechanism was suggested as static quenching according to the Stern-Volmer equation. The binding constants
(K) at different temperatures as well as thermodynamic parameters, enthalpy change (ΔH) and entropy change (ΔS), were calculated according to relevant fluorescent data and Van’t Hoff equation. The hydrophobic interaction was a predominant
intermolecular force in order to stabilize the complex, which was in agreement with the results of molecular modeling study.
In addition, the effects of other ions on the binding constants were also studied. Moreover, the synchronous fluorescence
technique was successfully employed to determine the total proteins in serum, urine and saliva samples at room temperature
under the optimum conditions with a wide linear range and satisfactory results. 相似文献
The structures of Ga_3N, GaN_3, Ga_3N_2 and Ga_2N_3 clusters are studied using the full-potential linear-muffin-tin-orbital molecular dynamics (FP-LMTO MD) method. Four structures for Ga_3 N, five structures for GaN_3, nine structures for Ga_3N_2 and nine structures for Ga_2N_3 have been obtained. The most stable structures of these clusters are planar ones. A strong dominance of the N--N bond over the Ga--N and Ga--Ga bonds appears to control the structural skeletons, supporting the previous result obtained by Kandalam and co-workers. The most stable structures of these small GaN clusters displayed semiconductor-like properties through the calculation of the HOMO-LUMO gaps. 相似文献